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1. 发明申请

WO2013102091A1 NUCLEIC ACID LIGATION SYSTEMS AND METHODS 审中-公开
标题翻译：核酸配位体系及方法
公开(公告)号：WO2013102091A1
公开(公告)日：2013-07-04
申请号：PCT/US2012/072089
申请日：2012-12-28
申请人： IBIS BIOSCIENCES, INC. , ESHOO, Mark W. , PICURI, John , MOTLEY, Stanley , PHILLIPSON, Curtis
发明人： ESHOO, Mark W. , PICURI, John , MOTLEY, Stanley , PHILLIPSON, Curtis
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6855 , C12N15/10 , C12N15/11 , C12N15/66 , C12P19/34 , C12Q1/6869 , C12Q2525/191 , C12Q2525/301 , C12Q2537/143
摘要： The present disclosure relates to systems and methods for nucleic acid ligation. In particular, the present disclosure provides oligonucleotide adaptors for use in nucleic acid ligation reactions.
摘要翻译：本公开涉及用于核酸连接的系统和方法。特别地，本公开提供用于核酸连接反应的寡核苷酸衔接子。

2. 发明申请

WO2012092433A1 RAPID WHOLE GENOME AMPLIFICATION 审中-公开
标题翻译：快速全基因扩增
公开(公告)号：WO2012092433A1
公开(公告)日：2012-07-05
申请号：PCT/US2011/067739
申请日：2011-12-29
申请人： IBIS BIOSCIENCES, INC. , ESHOO, Mark W. , MOTLEY, Stanley , PICURI, John
发明人： ESHOO, Mark W. , MOTLEY, Stanley , PICURI, John
IPC分类号： C12P19/34
CPC分类号： C12Q1/686 , C12P19/34 , C12Q1/6846 , C12Q2521/525 , C12Q2525/179 , C12Q2527/125
摘要： The present invention provides compositions and methods for rapidly amplifying target nucleic acid (e.g., using whole genome amplification) that allows small amounts of starting nucleic acid to be employed. In certain embodiments, the methods employ compositions that comprise: phi29 polymerase, exo- Klenow polymerase and/or Klenow polymerase, dNTPs, primers, and a buffering agent. In some embodiments, the target nucleic acid is amplified at a rate that would result in at least 1000-fold amplification in thirty minutes.
摘要翻译：本发明提供用于快速扩增允许使用少量起始核酸的靶核酸（例如，使用全基因组扩增）的组合物和方法。在某些实施方案中，所述方法使用包含以下的组合物：phi29聚合酶，ex-Klenow聚合酶和/或Klenow聚合酶，dNTP，引物和缓冲剂。在一些实施方案中，以在30分钟内至少产生1000倍扩增的速率扩增靶核酸。

3. 发明申请

WO2012100194A1 MICROFLUIDIC TRANSDUCER 审中-公开
标题翻译：微流量传感器
公开(公告)号：WO2012100194A1
公开(公告)日：2012-07-26
申请号：PCT/US2012/022077
申请日：2012-01-20
申请人： IBIS BIOSCIENCES, INC. , ESHOO, Mark W. , GUTIERREZ, Jose R. , DRADER, Jared J. , PICURI, John , CABRERA, Karl M. , MOTLEY, Stanley
发明人： ESHOO, Mark W. , GUTIERREZ, Jose R. , DRADER, Jared J. , PICURI, John , CABRERA, Karl M. , MOTLEY, Stanley
IPC分类号： C12N13/00 , C12M1/33
CPC分类号： G01N1/31 , B01L3/502715 , B01L2200/027 , B01L2300/06 , B01L2300/0816 , C12M47/06 , C12N1/066 , C12N13/00 , C12Q1/6806 , G01N1/30 , Y10T436/25
摘要： Methods and apparatus for fragmenting nucleic acids or disrupting cells in a disposable microfluidic device are disclosed. The apparatus is designed to position a sample to be in direct contact with a high frequency vibrating element that emits an ultrasonic frequency into the sample such that the vibrational energy transduced into the sample results in fragmenting nucleic acids or disrupting cells.
摘要翻译：公开了在一次性微流体装置中分裂核酸或破坏细胞的方法和装置。该设备被设计成将样品定位成与向发射超声波频率的高频振动元件直接接触，使得转换到样品中的振动能量导致碎裂的核酸或破坏细胞。

4. 发明申请

WO2011140489A2 INTEGRATED SAMPLE PREPARATION SYSTEMS AND STABILIZED ENZYME MIXTURES 审中-公开
标题翻译：集成样品制备系统和稳定的酶混合物
公开(公告)号：WO2011140489A2
公开(公告)日：2011-11-10
申请号：PCT/US2011/035597
申请日：2011-05-06
申请人： IBIS BIOSCIENCES, INC. , ESHOO, Mark, W. , PICURI, John
发明人： ESHOO, Mark, W. , PICURI, John
IPC分类号： C40B60/14 , C12N9/96
CPC分类号： B01L3/5027 , B01L3/502753 , B01L7/52 , B01L9/527 , B01L2200/10 , B01L2200/16 , B01L2300/0672 , B01L2300/0681 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2300/087 , B01L2300/0887 , B01L2400/0481 , B01L2400/0487 , B01L2400/0655 , B01L2400/0683 , C12N9/96 , C12Q1/6806 , C12Q1/6874
摘要： The present invention provides integrated sample preparation systems and stabilized enzyme mixtures. In particular, the present invention provides microfluidic cards configured for processing a sample and generating DNA libraries that are suitable for use in sequencing methods (e.g., next generation sequencing methods) or other suitable nucleic acid analysis methods. The present invention also provides stabilized enzyme mixtures containing an enzyme (e.g., an enzyme used in whole genome amplification), BSA, and a sugar. Such enzyme mixtures may be lyophilized and stored at room temperature without significant loss of enzyme activity for months.
摘要翻译：本发明提供了综合的样品制备系统和稳定的酶混合物。特别地，本发明提供了配置用于处理样品并生成适合用于测序方法（例如，下一代测序方法）或其他合适的核酸分析方法的DNA文库的微流控卡。本发明还提供了含有酶（例如，用于全基因组扩增的酶），BSA和糖的稳定的酶混合物。这样的酶混合物可以冻干并在室温下储存，而不会显着损失酶活性数月。

5. 发明申请

WO2013102093A1 MULTIPLE- ANALYTE ASSAY DEVICE AND SYSTEM 审中-公开
标题翻译：多分析仪器和系统
公开(公告)号：WO2013102093A1
公开(公告)日：2013-07-04
申请号：PCT/US2012/072091
申请日：2012-12-28
申请人： IBIS BIOSCIENCES, INC. , ESHOO, Mark W. , PICURI, John
发明人： ESHOO, Mark W. , PICURI, John
IPC分类号： C12M1/34 , C12Q1/00 , G01N33/487
CPC分类号： B01L3/502769 , B01L7/00 , B01L2200/06 , B01L2200/10 , G01N33/54366 , G01N2035/00158
摘要： Provided herein are systems and methods for performing complex chemical, physical and biological assays. In particular, provided herein are systems and methods for performing microfluidic assays.
摘要翻译：本文提供了用于进行复杂化学，物理和生物测定的系统和方法。特别地，本文提供了用于进行微流体测定的系统和方法。

6. 发明申请

WO2012092260A1 COMPOSITIONS AND METHODS FOR PRODUCING SINGLE-STRANDED CIRCULAR DNA 审中-公开
标题翻译：用于生产单链圆形DNA的组合物和方法
公开(公告)号：WO2012092260A1
公开(公告)日：2012-07-05
申请号：PCT/US2011/067368
申请日：2011-12-27
申请人： IBIS BIOSCIENCES, INC. , ESHOO, Mark W. , PICURI, John
发明人： ESHOO, Mark W. , PICURI, John
IPC分类号： C07H21/04 , C12Q1/68
CPC分类号： C12Q1/686 , B01L7/52 , C12N15/10 , C12N15/66 , C12P19/34
摘要： The present invention provides methods, kits, and compositions for producing single-stranded circular DNA by PCR. In particular, hairpin primers are provided, and methods of use thereof to produce single-stranded circular DNA molecules.
摘要翻译：本发明提供了通过PCR产生单链环状DNA的方法，试剂盒和组合物。特别地，提供发夹引物及其用于产生单链环状DNA分子的方法。

7. 发明申请

WO2011047307A1 MULTIPLE DISPLACEMENT AMPLIFICATION 审中-公开
标题翻译：多位置放大
公开(公告)号：WO2011047307A1
公开(公告)日：2011-04-21
申请号：PCT/US2010/052904
申请日：2010-10-15
申请人： IBIS BIOSCIENCES, INC. , ESHOO, Mark W. , PICURI, John , PHILLIPSON, Curtis
发明人： ESHOO, Mark W. , PICURI, John , PHILLIPSON, Curtis
IPC分类号： C12P19/34
CPC分类号： C12P19/34 , C12Q1/6844 , C12Q1/686 , C12Q2527/125 , C12Q2531/119 , C12Q2537/143 , C12Q2563/159 , C12Q2527/127 , C12Q2531/113
摘要： The present invention provides methods kits and systems for performing multiple displacement amplification reactions. In one method a sample of nucleic acid is provided. The nucleic acid is contacted with a reaction mixture which includes a set of oligonucleotide primers, a one or more polymerase enzymes and a detergent. The reaction mixture is then subjected to conditions under which the nucleic acid sequence is amplified to produce an amplified product in a multiple displacement reaction. The method may also be carried out by contacting the nucleic acid with the reaction mixture in the form of an emulsion. A kit is also provided for carrying out either the methods described above. The kit includes one or more polymerases, a plurality of primers and a detergent. The kit may also include a hydrophobic polymer and may include instructions for performing a multiple displacement amplification reaction on a nucleic acid sample.
摘要翻译：本发明提供了用于进行多重置换扩增反应的方法试剂盒和系统。在一种方法中，提供了核酸样品。将核酸与包含一组寡核苷酸引物，一种或多种聚合酶和洗涤剂的反应混合物接触。然后将反应混合物进行条件，在该条件下扩增核酸序列以在多重置换反应中产生扩增产物。该方法还可以通过使乳酸与乳液形式的反应混合物接触来进行。还提供了用于执行上述方法的试剂盒。试剂盒包括一种或多种聚合酶，多种引物和洗涤剂。试剂盒还可以包括疏水性聚合物，并且可以包括在核酸样品上进行多重置换扩增反应的说明书。

8. 发明申请

WO2016065299A3 ULTRASONICS FOR MICROFLUIDIC SAMPLE PREPARATION 审中-公开
标题翻译：用于微流体样品制备的超声波
公开(公告)号：WO2016065299A3
公开(公告)日：2016-08-11
申请号：PCT/US2015057184
申请日：2015-10-23
申请人： ESHOO MARK W , PICURI JOHN , HANG ALICE T , SMITH HEATHER L , MEIJERING BERNADET , KRAGT RENE , BRUYNINCKX M , DOBBELAER I
发明人： ESHOO MARK W , PICURI JOHN , HANG ALICE T , SMITH HEATHER L , MEIJERING BERNADET , KRAGT RENE , BRUYNINCKX M , DOBBELAER I
IPC分类号： G01N33/48 , B02C19/18 , G01N33/49
CPC分类号： C12M47/06
摘要： Provided herein is technology relating to microfluidics and particularly, but not exclusively, to devices, apparatuses, methods, and systems for preparing and analyzing biomolecules using an ultrasonic device.
摘要翻译：本文提供了涉及微流体的技术，特别但并非排他地涉及使用超声波装置制备和分析生物分子的装置，装置，方法和系统。

9. 发明申请

WO2012044956A1 TARGETED GENOME AMPLIFICATION METHODS 审中-公开
标题翻译：目标基因扩增方法
公开(公告)号：WO2012044956A1
公开(公告)日：2012-04-05
申请号：PCT/US2011/054262
申请日：2011-09-30
申请人： IBIS BIOSCIENCES, INC. , ESHOO, Mark W. , CROWDER, Christopher , PICURI, John , WHITE, Neill , ROUNDS, Megan A.
发明人： ESHOO, Mark W. , CROWDER, Christopher , PICURI, John , WHITE, Neill , ROUNDS, Megan A.
IPC分类号： C12Q1/68
CPC分类号： C12Q1/686 , C12Q2531/119 , C12Q2537/159
摘要： The methods disclosed herein relate to methods and compositions for amplifying nucleic acid sequences, more specifically, from nucleic acid sequences of pathogens by targeted genome amplification. In certain embodiments, multiple primer pairs are employed that flank a target region and polymerization is conducted with a strand displacing enzyme.
摘要翻译：本文公开的方法涉及用于通过靶向基因组扩增来更好地从核酸序列扩增核酸序列的方法和组合物。在某些实施方案中，使用在靶区域侧面的多个引物对，并且用链置换酶进行聚合。

10. 发明申请

WO2011112718A1 PRODUCTION OF SINGLE-STRANDED CIRCULAR NUCLEIC ACID 审中-公开
标题翻译：生产单条圆形核酸
公开(公告)号：WO2011112718A1
公开(公告)日：2011-09-15
申请号：PCT/US2011/027753
申请日：2011-03-09
申请人： IBIS BIOSCIENCES, INC. , ESHOO, Mark W. , PICURI, John
发明人： ESHOO, Mark W. , PICURI, John
IPC分类号： C12P19/34 , C07H21/04 , C12Q1/68
CPC分类号： C12N15/10 , C12N9/22 , C12N15/66 , C12P19/34 , C12Q2521/501 , C12Q2521/519 , C12Q2525/301 , C12Q2525/307 , C40B40/08 , C40B50/06
摘要： A method is provided for generating single stranded circular nucleic acid from a sample of target nucleic acid. A complex comprising a transposase and a plurality of hairpin polynucleotides is formed with each of the hairpin polynucleotides having a duplex region comprising a transposase recognition sequence. The complex is mixed with the target nucleic acid, thereby fragmenting the target nucleic acid and ligating the hairpin polynucleotides to the target nucleic acid to form hairpin-linked nucleic acid fragments, each having a nucleobase segment gap between each fragment and its corresponding hairpin polynucleotide. The hairpin-linked fragments are contacted with a ligase, thereby ligating the hairpin-linked fragments together to form single-stranded circular nucleic acid comprising a pair of opposing loops and an intervening duplex region comprising a pair of nucleobase segment gaps. The single-single stranded circular nucleic acid is then contacted with a polymerase and nucleotide triphosphates, thereby filling the nucleobase segment gaps.
摘要翻译：提供了用于从靶核酸样品产生单链环状核酸的方法。形成包含转座酶和多个发夹多核苷酸的复合物，每个发夹多核苷酸具有包含转座酶识别序列的双链体区域。将复合物与靶核酸混合，从而使靶核酸分裂，并将发夹多核苷酸连接到靶核酸上以形成发夹连接的核酸片段，每个核酸片段在每个片段与其相应的发夹多核苷酸之间具有核碱基区段间隙。将发夹连接的片段与连接酶接触，从而将发夹连接的片段连接在一起形成包含一对相对环的单链环状核酸和包含一对核碱基区段间隙的中间双链体区域。然后单链单链核酸与聚合酶和核苷酸三磷酸酯接触，由此填充核碱基区段间隙。

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