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1. 发明申请

WO2013019411A1 METHODS AND COMPOSITIONS FOR TARGETED INTEGRATION IN A PLANT 审中-公开
标题翻译：方法和组合在工厂中进行集中整合
公开(公告)号：WO2013019411A1
公开(公告)日：2013-02-07
申请号：PCT/US2012/047202
申请日：2012-07-18
申请人： E. I. DU PONT DE NEMOURS AND COMPANY , PIONEER HI-BRED INTERNATIONAL , CIGAN, Andrew Mark , FALCO, Saverio Carl , GAO, Huirong , LASSNER, Michael W. , LI, Zhongsen , LIU, Zhan-Bin , LYZNIK, Leszek A. , PETERSON, David J. , SCELONGE, Christopher Jay
发明人： CIGAN, Andrew Mark , FALCO, Saverio Carl , GAO, Huirong , LASSNER, Michael W. , LI, Zhongsen , LIU, Zhan-Bin , LYZNIK, Leszek A. , PETERSON, David J. , SCELONGE, Christopher Jay
IPC分类号： C12N15/82 , A01H5/00
CPC分类号： C12N15/8213
摘要： Compositions and methods are provided for the targeted integration of a polynucleotide sequence of interest into the genome of a plant or plant cell. The methods and compositions employ recognition sites for endonucleases and endonucleases in combination with site-specific recombination sites/recombinases to provide an effective system for establishing target sites within the genome of a plant, plant cell or seed. Once such target sites are established, a variety of methods can be employed to further modify the target sites such that they contain a variety of polynucleotides of interest.
摘要翻译：提供组合物和方法用于将目的多核苷酸序列靶向整合到植物或植物细胞的基因组中。方法和组合物使用核酸内切酶和内切核酸酶的识别位点与位点特异性重组位点/重组酶组合，以提供用于在植物，植物细胞或种子的基因组内建立靶位点的有效系统。一旦建立了这样的靶位点，就可以采用多种方法来进一步修饰靶位点，使得它们含有多种感兴趣的多核苷酸。

2. 发明申请

WO2005057422A3 INTEGRATED MOBILE RESOURCE SYSTEM 审中-公开
公开(公告)号：WO2005057422A3
公开(公告)日：2005-06-23
申请号：PCT/US2004/041123
申请日：2004-12-08
申请人： ERBUS, INC. , YUNGNER, Deborah , MENDEZ, Frank, G. , PETERSON, David, J.
发明人： YUNGNER, Deborah , MENDEZ, Frank, G. , PETERSON, David, J.
IPC分类号： B60P3/00
摘要： An integrated and modular infrastructure resource system (IRS) is herein described. The IRS includes one or more infrastructure modules adapted to fulfill a given infrastructure needs such as electrical power, communications, water purification, and air filtration. The IRS may be controlled locally by a control module coupled to the infrastructure modules by a power bus and a control bus or remotely by a remote control-panel that communicates with the IRS by means of a communications module that includes a two-way communications device. The power bus and control bus act to network all of the provided infrastructure modules into an integrated system. Maintenance and logistics for a plurality of IRS's may be managed remotely by a single remote control panel.

3. 发明申请

WO2011082310A3 METHODS AND COMPOSITIONS FOR TARGETED POLYNUCLEOTIDE MODIFICATION 审中-公开
标题翻译：目标多核苷酸修饰的方法和组合物
公开(公告)号：WO2011082310A3
公开(公告)日：2011-09-01
申请号：PCT/US2010062518
申请日：2010-12-30
申请人： PIONEER HI BRED INT , GORDON-KAMM WILLIAM J , LOWE KEITH S , PETERSON DAVID J , SCELONGE CHRISTOPHER J , ST CLAIR GRACE M , WANG BING-BING
发明人： GORDON-KAMM WILLIAM J , LOWE KEITH S , PETERSON DAVID J , SCELONGE CHRISTOPHER J , ST CLAIR GRACE M , WANG BING-BING
IPC分类号： C12N15/82 , A01H5/00 , C07K14/415
CPC分类号： C12N15/8216 , C07K14/415 , C12N15/8213
摘要： A variety of methods and compostions are provided, including methods and compositions for targeted modification of a specific target site in a cell or organism, methods for integrating polynucleotides of interest, methods to assess promoter activity, directly select transformed organisms, minimize or eliminate expression resulting from random integration into the genome of an organism, such as a plant, remove polynucleotides of interest, combine multiple transfer cassettes, invert or excise a polynucleotide, silence a gene, and identify and/or characterize transcriptional regulating regions. The methods involve the introduction of a cell proliferation factor and a double-strand break-inducing enzyme into an organism.
摘要翻译：提供了多种方法和组合物，包括用于靶向修饰细胞或生物体中特定靶位点的方法和组合物，整合目标多核苷酸的方法，评估启动子活性的方法，直接选择转化的生物体，最小化或消除表达产物从随机整合到生物体（例如植物）的基因组中，去除感兴趣的多核苷酸，组合多个转移盒，转化或切除多核苷酸，沉默基因，以及鉴定和/或表征转录调控区。这些方法涉及将细胞增殖因子和双链断裂诱导酶引入生物体中。

4. 发明申请

WO2011082310A2 METHODS AND COMPOSITIONS FOR TARGETED POLYNUCLEOTIDE MODIFICATION 审中-公开
标题翻译：用于靶向多核苷酸修饰的方法和组合物
公开(公告)号：WO2011082310A2
公开(公告)日：2011-07-07
申请号：PCT/US2010/062518
申请日：2010-12-30
申请人： PIONEER HI-BRED INTERNATIONAL, INC. , GORDON-KAMM, William, J. , LOWE, Keith, S. , PETERSON, David, J. , SCELONGE, Christopher, J. , ST. CLAIR, Grace, M. , WANG, Bing-Bing
发明人： GORDON-KAMM, William, J. , LOWE, Keith, S. , PETERSON, David, J. , SCELONGE, Christopher, J. , ST. CLAIR, Grace, M. , WANG, Bing-Bing
IPC分类号： C12N15/82
CPC分类号： C12N15/8216 , C07K14/415 , C12N15/8213
摘要： A variety of methods and compostions are provided, including methods and compositions for targeted modification of a specific target site in a cell or organism, methods for integrating polynucleotides of interest, methods to assess promoter activity, directly select transformed organisms, minimize or eliminate expression resulting from random integration into the genome of an organism, such as a plant, remove polynucleotides of interest, combine multiple transfer cassettes, invert or excise a polynucleotide, silence a gene, and identify and/or characterize transcriptional regulating regions. The methods involve the introduction of a cell proliferation factor and a double-strand break-inducing enzyme into an organism.
摘要翻译：提供了各种方法和组合物，包括用于靶向修饰细胞或生物体中特定靶位点的方法和组合物，用于整合感兴趣的多核苷酸的方法，评估启动子活性的方法，直接选择转化的生物体，最小化或消除产生的表达从随机整合到诸如植物的生物体的基因组中，去除感兴趣的多核苷酸，组合多个转移盒，反转或切割多核苷酸，使基因沉默，并鉴定和/或表征转录调节区。该方法涉及将细胞增殖因子和双链断裂诱导酶引入生物体。

5. 发明申请

WO2005057422A2 INTEGRATED MOBILE RESOURCE SYSTEM 审中-公开
标题翻译：集成的移动资源系统
公开(公告)号：WO2005057422A2
公开(公告)日：2005-06-23
申请号：PCT/US2004041123
申请日：2004-12-08
申请人： ERBUS INC , YUNGNER DEBORAH , MENDEZ FRANK G , PETERSON DAVID J
发明人： YUNGNER DEBORAH , MENDEZ FRANK G , PETERSON DAVID J
IPC分类号： B60P3/00 , B60P3/14 , G06F17/00
CPC分类号： B60P3/14 , Y02W10/37
摘要： An integrated and modular infrastructure resource system (IRS) is herein described. The IRS includes one or more infrastructure modules adapted to fulfill a given infrastructure needs such as electrical power, communications, water purification, and air filtration. The IRS may be controlled locally by a control module coupled to the infrastructure modules by a power bus and a control bus or remotely by a remote control-panel that communicates with the IRS by means of a communications module that includes a two-way communications device. The power bus and control bus act to network all of the provided infrastructure modules into an integrated system. Maintenance and logistics for a plurality of IRS's may be managed remotely by a single remote control panel.
摘要翻译：这里描述了一个集成和模块化的基础设施资源系统（IRS）。 IRS包括一个或多个适用于满足给定基础设施需求的基础设施模块，例如电力，通信，水净化和空气过滤。 IRS可由通过电力总线和控制总线耦合到基础设施模块的控制模块进行本地控制，或者通过远程控制面板远程控制，所述远程控制面板通过通信模块与IRS通信，所述通信模块包括双向通信设备。电力总线和控制总线将所有提供的基础设施模块联网成一个集成系统。多个IRS的维护和后勤可通过单个远程控制面板远程管理。

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