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    • 6. 发明申请
    • METHOD FOR OBTAINING A MONOCOTYLEDON PLANT CONTAINING A GENE OF INTEREST FREE OF FOREIGN ANCILLARY SEQUENCE
    • 获取含有免费外国序列的基因的单克隆植物的方法
    • WO02101061A8
    • 2005-04-28
    • PCT/FR0201656
    • 2002-05-16
    • RHOBIOPEREZ PASCUALGERENTES DENISEPRAUD SEBASTIEN
    • PEREZ PASCUALGERENTES DENISEPRAUD SEBASTIEN
    • C12N15/82A01H5/00A01H5/10C12Q1/68
    • C12N15/8213
    • The invention concerns a method for obtaining a transgenic monocotyledon plant containing a gene of interest free of foreign ancillary sequences, comprising the following steps: a) transforming at least a plant cell free of active transposase, with a vector comprising two expression cassettes, one (i) including a nucleotide sequence of interest, the other (ii) including a nucleotide sequence coding for a selection marker framed by the mobilisable sequences of a transposon; b) selecting plants transformed with the selection marker (ii); cross-breeding a transformed plant with another plant belonging to a line containing in its genome a gene, coding for an endogenous active transposon, and which is in the middle of an excision phenotypic marker (iii) to obtain a F1 or any other individual of subsequent generation; d) selecting cells of individual carriers of the gene of interest free of foreign ancillary sequence, from the F1 generation; e) regenerating plants from the cells or individuals selected in (d).
    • 本发明涉及一种获得含有不含外来辅助序列的目标基因的转基因单子叶植物的方法,其包括以下步骤:a)使用包含两个表达盒的载体转化至少一个没有活性转座酶的植物细胞,一个 i)包括感兴趣的核苷酸序列,另一个(ii)包括编码由转座子的可移动序列构建的选择标记的核苷酸序列; b)选择用选择标记(ii)转化的植物; 将转化的植物与属于其基因组中含有编码内源活性转座子的基因的另一种植物杂交育种,并且其位于切除表型标记的中间(iii)以获得F1或任何其他个体 后代 d)从F1代选择没有外来辅助序列的目的基因的个体载体的细胞; e)从(d)中选择的细胞或个体再生植物。