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1. 发明申请

WO1990012089A1 PROCESS FOR CLEANING ENZYME SOLUTIONS 审中-公开
标题翻译：清洁酶解决方法
公开(公告)号：WO1990012089A1
公开(公告)日：1990-10-18
申请号：PCT/EP1990000500
申请日：1990-03-29
申请人： HENKEL KOMMANDITGESELLSCHAFT AUF AKTIEN , BIOCHEMIE GESELLSCHAFT M.B.H. , WÜST, Willi , KÜHNE, Norbert , PALMA, Norbert
发明人： HENKEL KOMMANDITGESELLSCHAFT AUF AKTIEN , BIOCHEMIE GESELLSCHAFT M.B.H.
IPC分类号： C12N09/00
CPC分类号： C12N9/54 , C12N9/00
摘要： In a process for cleaning and deodorising aqueous enzyme solutions obtained by the fermentation of bacteria or fungi, the enzyme yield and the filterability of the deposits to be separated are to be improved. This is achieved by adding to the enzyme solution at least one masking agent selected from the following group: boric acids and/or their water-soluble salts; sulphurous acids and/or their water-soluble salts; hydroxycarboxylic acids and/or their water-soluble salts; multivalent alcohols; and thereafter producing a precipitate to which are added in any order two water-soluble, mutually precipitating ionic compounds with further adsorption agents if desired and then separating the solids from the enzyme solution.

2. 发明申请

WO2005019466A3 PROCESS FOR THE PREPARATION OF RECOMBINANT POLYPEPTIDES 审中-公开
公开(公告)号：WO2005019466A3
公开(公告)日：2005-03-03
申请号：PCT/EP2004/009321
申请日：2004-08-19
申请人： SANDOZ AG , STEMPFER, Günter , PALMA, Norbert
发明人： STEMPFER, Günter , PALMA, Norbert
IPC分类号： C12P21/02
摘要： The present invention relates to the preparation of a recombinant polypeptide, which polypeptide upon expression has been secreted into the periplasm of a transformed host cell. In particular, this invention relates to a process to enhance the extraction yield of said recombinant polypeptide from the periplasm before further downstream processing.

3. 发明申请

WO2005017174A2 PROCESS FOR THE PURIFICATION OF RECOMBINANT POLYPEPTIDES 审中-公开
标题翻译：重组多肽的纯化方法
公开(公告)号：WO2005017174A2
公开(公告)日：2005-02-24
申请号：PCT/EP2004/009055
申请日：2004-08-12
申请人： SANDOZ AG , STEMPFER, Günter , ALLIGER, Peter , PALMA, Norbert
发明人： STEMPFER, Günter , ALLIGER, Peter , PALMA, Norbert
IPC分类号： C12P21/00
CPC分类号： C07K14/56 , C12P21/02
摘要： This invention relates to a method for purification of a recombinant polypeptide of interest, which polypeptide upon expression has been secreted into the periplasm of a transformed host cell.
摘要翻译：本发明涉及用于纯化感兴趣的重组多肽的方法，所述多肽在表达时已经被分泌到转化的宿主细胞的周质中。

4. 发明申请

WO2005017162A3 EXPRESSION VECTORS, TRANSFORMED HOST CELLS AND FERMENTATION PROCESS FOR THE PRODUCTION OF RECOMBINANT POLYPEPTIDES 审中-公开
公开(公告)号：WO2005017162A3
公开(公告)日：2005-02-24
申请号：PCT/EP2004/009067
申请日：2004-08-12
申请人： SANDOZ AG , WINDISCH, Jörg , SCHOERGENDORFER, Kurt , PALMA, Norbert , KNAUSEDER, Franz , BOEHLING, Hans
发明人： WINDISCH, Jörg , SCHOERGENDORFER, Kurt , PALMA, Norbert , KNAUSEDER, Franz , BOEHLING, Hans
IPC分类号： C12N15/63
摘要： The present invention relates to an expression vector, comprising a polynucleotide encoding a fusion protein comprising the signal sequence of the gac gene of Pseudomonas diminuta and a polypeptide of interest, a prokaryotic host cell transformed with such an expression vector and a process for production of a polypeptide of interest using said host cell and said expression vector.

5. 发明申请

WO9913058A3 ESTERASE FREE ENZYMES 审中-公开
标题翻译：酯酶免疫酶
公开(公告)号：WO9913058A3
公开(公告)日：1999-06-10
申请号：PCT/EP9805729
申请日：1998-09-08
申请人： BIOCHEMIE GMBH , REICHERT ARNO , RIETHORST WAANDER , KNAUSEDER FRANZ , PALMA NORBERT
发明人： REICHERT ARNO , RIETHORST WAANDER , KNAUSEDER FRANZ , PALMA NORBERT
IPC分类号： C12N9/98 , C12N9/06 , C12N9/80 , C12N11/00 , C12N11/04 , C12P35/00
CPC分类号： C12N9/0024 , C12N9/80 , C12N11/00 , C12N11/04 , C12P35/00
摘要： A process for decreasing esterase activity present in the presence of D-amino acid oxidase activity or in the presence of glutarylacylase activity in a mixture having esterase activity and D-amino acid oxidase activity and/or having esterase activity and glutarylacylase activity by treatment with phenylmethylsulphonyl fluoride; immobilized biocatalysts in spherical particle form obtainable by treating microorganism cells having enzyme activity with a primary or secondary amine containing polymer, an organic solvent which is able to form a two-phase system with water and a bifunctional agent; and the use of that process and biocatalysts in the production of 7-aminocephalo-sporanic acid, 7-amino-3-hydroxymethyl-3-cephem-4-carboxylic acid and cephalosporin antibiotics.
摘要翻译：在具有酯酶活性和D-氨基酸氧化酶活性和/或具有酯酶活性和戊二酰半胱氨酸酶活性的混合物中，通过用苯甲基磺酰基处理降低在D-氨基酸氧化酶活性存在下或在戊二酰基酶活性存在下存在的酯酶活性的方法氟化物; 通过用含有伯胺或仲胺的聚合物处理具有酶活性的微生物细胞获得的球形颗粒形式的固定化生物催化剂，能够与水和双官能剂形成两相体系的有机溶剂; 以及该方法和生物催化剂在生产7-氨基头孢-3-甲酸，7-氨基-3-羟甲基-3-头孢烯-4-羧酸和头孢菌素抗生素中的用途。

6. 发明申请

WO2005019466A2 PROCESS FOR THE PREPARATION OF RECOMBINANT POLYPEPTIDES 审中-公开
标题翻译：制备重组多肽的方法
公开(公告)号：WO2005019466A2
公开(公告)日：2005-03-03
申请号：PCT/EP2004009321
申请日：2004-08-19
申请人： SANDOZ AG , STEMPFER GUENTER , PALMA NORBERT
发明人： STEMPFER GUENTER , PALMA NORBERT
IPC分类号： C12P21/02 , C12P21/00
CPC分类号： C12P21/02
摘要： The present invention relates to the preparation of a recombinant polypeptide, which polypeptide upon expression has been secreted into the periplasm of a transformed host cell. In particular, this invention relates to a process to enhance the extraction yield of said recombinant polypeptide from the periplasm before further downstream processing.
摘要翻译：本发明涉及重组多肽的制备，所述多肽在表达时已被分泌到转化的宿主细胞的周质中。特别地，本发明涉及在进一步下游处理之前从周质中提高所述重组多肽的提取产率的方法。

7. 发明申请

WO2005017162A2 EXPRESSION VECTORS, TRANSFORMED HOST CELLS AND FERMENTATION PROCESS FOR THE PRODUCTION OF RECOMBINANT POLYPEPTIDES 审中-公开
标题翻译：表达载体，转化的宿主细胞和用于生产重组多肽的发酵方法
公开(公告)号：WO2005017162A2
公开(公告)日：2005-02-24
申请号：PCT/EP2004009067
申请日：2004-08-12
申请人： SANDOZ AG , WINDISCH JOERG , SCHOERGENDORFER KURT , PALMA NORBERT , KNAUSEDER FRANZ , BOEHLING HANS
发明人： WINDISCH JOERG , SCHOERGENDORFER KURT , PALMA NORBERT , KNAUSEDER FRANZ , BOEHLING HANS
IPC分类号： C07K14/56 , C12N15/63
CPC分类号： C12N15/70 , C07K14/56 , C07K2319/02 , C07K2319/034
摘要： The present invention relates to an expression vector, comprising a polynucleotide encoding a fusion protein comprising the signal sequence of the gac gene of Pseudomonas diminuta and a polypeptide of interest, a prokaryotic host cell transformed with such an expression vector and a process for production of a polypeptide of interest using said host cell and said expression vector.
摘要翻译：本发明涉及表达载体，其包含编码融合蛋白的多核苷酸，所述融合蛋白包含缺陷假单胞菌gac基因的信号序列和感兴趣的多肽，用这种表达载体转化的原核宿主细胞和生产使用所述宿主细胞和所述表达载体的目标多肽。

8. 发明申请

WO2003045996A1 CHROMATOGRAPHIC PURIFICATION OF RECOMBINANT HUMAN ERYTHROPOIETIN 审中-公开
标题翻译：重组人ERYTHROPOIETIN的色谱纯化
公开(公告)号：WO2003045996A1
公开(公告)日：2003-06-05
申请号：PCT/EP2002/013299
申请日：2002-11-26
申请人： BIOCHEMIE GESELLSCHAFT M.B.H. , ALLIGER, Peter , PALMA, Norbert
发明人： ALLIGER, Peter , PALMA, Norbert
IPC分类号： C07K14/505
CPC分类号： C07K14/505
摘要： The invention provides a method for recovering and purifying recombinant human erythropoietin (rhEpo) from a cell culture medium comprising host cells, which method comprises the steps of: (a) removing host cells, cellular constituents and debris from the cell culture medium by centrifugation using a disc stack separator followed by a depeth filtration step to obtain a clarified culture medium supernatant; (b) adjusting the conductivity of the supernatant to 5 mS/cm or less, and a pH of between about 7.0 and 8.0; (c) applying the supernatant from step (b) to a column comprising an anion exchange chromatographic medium, washing the column, eluting the rhEpo from the column, and collecting the peak fraction (s) that contain rhEpo; (d) subjecting the combined peak fractions from step (c) to a reverse phase chromatography step using a polystyrene resin that can be run under medium pressure (
摘要翻译：本发明提供了从包含宿主细胞的细胞培养基中回收和纯化重组人促红细胞生成素（rhEpo）的方法，该方法包括以下步骤：（a）通过离心从细胞培养基中除去宿主细胞，细胞成分和碎片，盘叠分离器，然后进行去过滤步骤以获得澄清的培养基上清液; （b）将上清液的电导率调节至5mS / cm或更低，pH值在约7.0至8.0之间; （c）将来自步骤（b）的上清液施加到包含阴离子交换色谱介质的柱中，洗涤柱，从柱洗脱rhEpo，并收集含有rhEpo的峰分数; （d）使用可在中压（<10巴）下运行并且耐高浓度NaOH（例如Source 30RPC）的聚苯乙烯树脂使步骤（c）的组合峰部分进行反相层析步骤，使用有机溶剂的线性梯度洗脱rhEpo; （e）将含有rhEpo的步骤（d）中洗脱的一种或多种级分施加到包含Q-Seph HP阴离子交换色谱介质的柱上，洗涤柱，并使用线性盐梯度洗脱rhEpo; （f）基于rhEpo的唾液酸化度选择在步骤（e）中洗脱的含有rhEpo的一个或多个级分; 和（g）使用Superdex 75制备级使一种或多种尺寸排阻层析步骤使含有rhEpo的步骤（f）中洗脱的一种或多种级分经脱水以除去潜在的二聚体和较高聚集体; 并收集含有rhEpo的洗脱液。

9. 发明申请

WO1989009821A1 GENE AND GENE PRODUCT FOR PREPARING 'beta'-LACTAM COMPOUNDS 审中-公开
标题翻译：用于制备β-LACTAM化合物的基因和基因产物
公开(公告)号：WO1989009821A1
公开(公告)日：1989-10-19
申请号：PCT/EP1989000374
申请日：1989-04-07
申请人： BIOCHEMIE GESELLSCHAFT M.B.H. , KNAUSEDER, Franz , LEITNER, Ernst , PALMA, Norbert , WEBER, Gerhard
发明人： BIOCHEMIE GESELLSCHAFT M.B.H.
IPC分类号： C12N15/00
CPC分类号： C12N9/84 , C12N9/1029
摘要： Gene and gene product of a key enzyme which is novel because of its structure and multivalent functionality, and catalyses the last stage of the biosynthesis of penicillin G and penicillin V.

10. 发明申请

WO2005017174A3 PROCESS FOR THE PURIFICATION OF RECOMBINANT POLYPEPTIDES 审中-公开
标题翻译：重组多糖的纯化方法
公开(公告)号：WO2005017174A3
公开(公告)日：2005-08-18
申请号：PCT/EP2004009055
申请日：2004-08-12
申请人： SANDOZ AG , STEMPFER GUENTER , ALLIGER PETER , PALMA NORBERT
发明人： STEMPFER GUENTER , ALLIGER PETER , PALMA NORBERT
IPC分类号： C07K14/56 , C12N1/06 , C12P21/00 , C12P21/02
CPC分类号： C07K14/56 , C12P21/02
摘要： This invention relates to a method for purification of a recombinant polypeptide of interest, which polypeptide upon expression has been secreted into the periplasm of a transformed host cell.
摘要翻译：本发明涉及一种用于纯化目标重组多肽的方法，该多肽在表达时已经分泌到转化的宿主细胞的周质中。

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