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    • 5. 发明授权
    • Analytical element and method for the determination of an analyte in a liquid
    • 用于测定液体中分析物的分析元素和方法
    • US06506575B1
    • 2003-01-14
    • US09667931
    • 2000-09-22
    • Wolfgang Reinhold KnappeRudolf PachlOtto Gaa
    • Wolfgang Reinhold KnappeRudolf PachlOtto Gaa
    • C12Q126
    • G01N33/54386G01N33/525
    • The invention concerns an analytical element for determining the amount of an analyte in a liquid containing a sample application zone (1) and a detection zone (2) which are in liquid-transferring contact, the latter containing at least one enzyme and an indicator substance, the enzyme catalysing a reaction in which the analyte or a substance derived from the analyte participates and the indicator substance forming a signal when the analyte is present which signal correlates with the amount of analyte, and containing a liquid-permeable interference-reducing layer (3) in direct contact with the detection zone (2) arranged such that liquid does not reach the detection zone until it has passed through the interference-reducing layer, the interference-reducing layer containing at least one enzyme which participates in a reaction of the analyte to be determined or of a substance derived from the analyte, wherein the analyte or the substance derived from the analyte is converted enzymatically in the interference-reducing layer into end products which cannot contribute to the signal formation by the indicator substance, and a method for the determination of an analyte in a liquid by means of such an analytical element.
    • 本发明涉及一种用于确定含有样品施用区(1)和液体转移接触的检测区(2)的液体中的分析物的量的分析元件,后者含有至少一种酶和指示剂物质 催化分析物或来自分析物的物质的反应的酶,当分析物存在时指示物质形成信号,该信号与分析物的量相关,并且含有液体可渗透的干扰减少层( 3)与检测区域(2)直接接触,其布置成使得液体在其通过干扰减少层之前不到达检测区域,干扰减少层含有至少一种参与反应的酶 待测定的分析物或衍生自分析物的物质,其中分析物或衍生自分析物的物质被转化为酶催化剂 y在干涉减少层中转化成不能通过指示剂物质对信号形成有贡献的终端产物,以及通过这种分析元件测定液体中的分析物的方法。