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    • 10. 发明申请
    • TRANSGENIC ORGANISM
    • 转基因生物
    • WO1994024292A2
    • 1994-10-27
    • PCT/EP1994001082
    • 1994-04-07
    • DANISCO A/SVILLAND, PerKLECZKOWSKI, LeszekOLSEN, Odd-ArnePOULSEN, PeterOKKELS, FinnMARCUSSEN, Jan
    • DANISCO A/S
    • C12N15/82
    • C12N9/1241C12N15/8245
    • A transgenic organism is described which has an increased starch yield. The preferred embodiment concerns a plant or plant cell containing a recombinant DNA construct containing, in operational relationship to a plant promoter sequence or sequences enabling the expression of the gene by the plant or plant cell thereby to enhance the rate of production and/or yield of starch by the plant or the plant cell, a DNA sequence encoding an exogenous ADP glucose pyrophosphorylase enzyme (AGP) or a sub-unit thereof which retains the enzymatic activity of the AGP enzyme, characterised in that the said DNA sequence is the gene sequence, including non-critical allelic variations thereof, encoding the barley (Hordeum vulgare) endosperm AGP or an active sub-unit thereof, or a variant thereof having non-critical amino acid substitution(s) or deletion(s) at one or more points in the amino acid sequences defining the barley endosperm AGP or either of its sub-units.
    • 描述了具有增加的淀粉产量的转基因生物。 优选实施方案涉及含有重组DNA构建体的植物或植物细胞,所述重组DNA构建体与植物启动子序列或序列具有操作关系,所述重组DNA构建体能够通过植物或植物细胞表达基因,从而提高生产和/或产量 植物或植物细胞的淀粉,编码保留AGP酶的酶活性的外源ADP葡萄糖焦磷酸化酶(AGP)或其亚单位的DNA序列,其特征在于所述DNA序列是基因序列, 包括其非重要等位基因变异,编码大麦(Hordeum vulgare)胚乳AGP或其活性亚单位或其在一个或多个点处具有非关键氨基酸取代或缺失的变体, 限定大麦胚乳AGP或其任何一个子单元的氨基酸序列。