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    • 2. 发明申请
    • ALKALINE PROTEASE
    • 碱性蛋白酶
    • WO2008111628A3
    • 2009-01-08
    • PCT/JP2008054562
    • 2008-03-06
    • KAO CORPOKUDA MITSUYOSHI
    • OKUDA MITSUYOSHI
    • C12N9/54C11D3/386
    • C11D3/386C12N9/54
    • It is an object of the present invention to provide alkaline proteases having industrially sufficient protein productivity and a significant detergency. In the alkaline proteases, the amino acid residues at (a) position 9, (b) position 49, (c) position 194, (d) position 212, (e) position 237, (f) position 245, (g) position 281, (h) position 313, (i) position 379 and (j) position 427 in SEQ ID NO: 2 are selected from the following amino acid residues; Position (a); glutamine, Position (b); glutamine, Position (c); lysine or arginine, Position (d); arginine, asparagine or glutamine, Position (e); asparagines, Position (f ); asparagines. Position (g); arginine, Position (h); asparagines, Position (i); lysine, arginine, glutamic acid or aspartic acid, and Position (j); arginine.
    • 本发明的目的是提供具有工业上足够的蛋白质生产力和显着去污力的碱性蛋白酶。 在碱性蛋白酶中,(a)位置9,(b)位置49,(c)位置194,(d)位置212,(e)位置237,(f)位置245,(g) 281,(h)位置313,(i)SEQ ID NO:2中的位置379和(j)位置427选自以下氨基酸残基; 职位(一); 谷氨酰胺,位置(b); 谷氨酰胺,位置(c); 赖氨酸或精氨酸,位置(d); 精氨酸,天冬酰胺或谷氨酰胺,位置(e); 天竺葵,位置(f); 天冬酰胺。 位置(g); 精氨酸,位置(h); 天竺葵,位置(i); 赖氨酸,精氨酸,谷氨酸或天冬氨酸,和位置(j); 精氨酸。
    • 3. 发明申请
    • ALKALINE PROTEASE
    • 碱性蛋白酶
    • WO2008111628A2
    • 2008-09-18
    • PCT/JP2008/054562
    • 2008-03-06
    • KAO CORPORATIONOKUDA, Mitsuyoshi
    • OKUDA, Mitsuyoshi
    • C12N9/54C11D3/386
    • C11D3/386C12N9/54
    • It is an object of the present invention to provide alkaline proteases having industrially sufficient protein productivity and a significant detergency. In the alkaline proteases, the amino acid residues at (a) position 9, (b) position 49, (c) position 194, (d) position 212, (e) position 237, (f) position 245, (g) position 281, (h) position 313, (i) position 379 and (j) position 427 in SEQ ID NO: 2 are selected from the following amino acid residues; Position (a); glutamine, Position (b); glutamine, Position (c); lysine or arginine, Position (d); arginine, asparagine or glutamine, Position (e); asparagines, Position (f ); asparagines. Position (g); arginine, Position (h); asparagines, Position (i); lysine, arginine, glutamic acid or aspartic acid, and Position (j); arginine.
    • 本发明的目的是提供具有工业上足够的蛋白质生产力和显着去污力的碱性蛋白酶。 在碱性蛋白酶中,(a)位置9,(b)位置49,(c)位置194,(d)位置212,(e)位置237,(f)位置245,(g)位置的氨基酸残基 281,(h)位置313,(i)SEQ ID NO:2中的位置379和(j)位置427选自以下氨基酸残基; 职位(一); 谷氨酰胺,位置(b); 谷氨酰胺,位置(c); 赖氨酸或精氨酸,位置(d); 精氨酸,天冬酰胺或谷氨酰胺,位置(e); 天竺葵,位置(f); 天冬酰胺。 位置(g); 精氨酸,位置(h); 天竺葵,位置(i); 赖氨酸,精氨酸,谷氨酸或天冬氨酸,和位置(j); 精氨酸。
    • 4. 发明申请
    • ALKALINE PROTEASE VARIANTS
    • 碱性蛋白酶变体
    • WO2010126156A2
    • 2010-11-04
    • PCT/JP2010/057838
    • 2010-04-26
    • KAO CORPORATIONTOHATA, MasatoshiOKUDA, MitsuyoshiSATO, TsuyoshiENDO, KeijiSAEKI, Katsuhisa
    • TOHATA, MasatoshiOKUDA, MitsuyoshiSATO, TsuyoshiENDO, KeijiSAEKI, Katsuhisa
    • C12N9/54
    • C11D3/38618C11D3/386C11D3/38681C12N9/54
    • An alkaline protease variant derived from an alkaline protease consisting of an amino acid sequence represented by SEQ ID NO: 2 or consisting of an amino acid sequence having an identity of 90% or more therewith, which variant has mutations wherein one or more amino acid residues at positions selected from (a) position 6, (b) position 15, (c) position 16, (d) position 65, (e) position 66, (f) position 82, (g) position 83, (h) position 204, (i) position 319, and (j) position 337 of the amino acid sequence represented by SEQ ID NO: 2, or at positions corresponding thereto are substituted with the following amino acid residues: (a) or a position corresponding thereto: Typ, Leu, Val, Ile, Met, Tyr, Gln, Lys, Thr, Phe, Arg, Ser, Cys, Ala, or His; (b) or a position corresponding thereto: Glu, Met, Asp, Val, Gln, Arg, Cys, Trp, Ala, or Phe; (c) or a position corresponding thereto: Met, Glu, Arg, Val, Lys, Phe, Tyr, Ile, His, Asp, or Cys; (d) or a position corresponding thereto: Trp; (e) or a position corresponding thereto: His, Trp, Ser, or Leu; (f) or a position corresponding thereto: Ala, Glu, Gln, Ser, Cys, Gly, His, Lys, Arg, Met, or Asn; (g) or a position corresponding thereto: Ala, Ser, or Cys; (h) or a position corresponding thereto: Glu, Asp, Cys, Val, Thr, Pro, His, Ile, Trp, Ser, Asn, Lys, or Arg; (i) or a position corresponding thereto: Trp, Val, Thr, Leu, Ile, Cys, Glu, Lys, Tyr, Arg, Phe, Gln, Met, Pro, Asp, Asn, His, or Ser; and (j) or a position corresponding thereto: Arg, Gly, Ser, Lys, Gln, Thr, His, Ala, Cys, or Val.
    • 由碱性蛋白酶衍生的碱性蛋白酶变体,其由SEQ ID NO:2所示的氨基酸序列组成或由与其同一性为90%以上的氨基酸序列组成,所述变体具有突变,其中一个或多个氨基酸残基 在从(a)位置6,(b)位置15,(c)位置16,(d)位置65,(e)位置66,(f)位置82,(g)位置83,(h) 204,(i)位置319和(j)由SEQ ID NO:2表示的氨基酸序列的位置337或与其相对应的位置被以下氨基酸残基取代:(a)或与其对应的位置: Typ,Leu,Val,Ile,Met,Tyr,Gln,Lys,Thr,Phe,Arg,Ser,Cys,Ala或His; (b)或相应位置:Glu,Met,Asp,Val,Gln,Arg,Cys,Trp,Ala或Phe; (c)或对应的位置:Met,Glu,Arg,Val,Lys,Phe,Tyr,Ile,His,Asp或Cys; (d)或与之对应的位置:Trp; (e)或与之对应的位置:His,Trp,Ser或Leu; (f)或对应的位置:Ala,Glu,Gln,Ser,Cys,Gly,His,Lys,Arg,Met或Asn; (g)或对应的位置:Ala,Ser或Cys; (h)或相应位置:Glu,Asp,Cys,Val,Thr,Pro,His,Ile,Trp,Ser,Asn,Lys或Arg; (i)或对应的位置:Trp,Val,Thr,Leu,Ile,Cys,Glu,Lys,Tyr,Arg,Phe,Gln,Met,Pro,Asp,Asn,His或Ser; 和(j)或对应的位置:Arg,Gly,Ser,Lys,Gln,Thr,His,Ala,Cys或Val。
    • 5. 发明申请
    • ALKALINE PROTEASE VARIANTS
    • 碱性蛋白酶变体
    • WO2010126156A3
    • 2011-01-06
    • PCT/JP2010057838
    • 2010-04-26
    • KAO CORPTOHATA MASATOSHIOKUDA MITSUYOSHISATO TSUYOSHIENDO KEIJISAEKI KATSUHISA
    • TOHATA MASATOSHIOKUDA MITSUYOSHISATO TSUYOSHIENDO KEIJISAEKI KATSUHISA
    • C12N9/54C11D3/386
    • C11D3/38618C11D3/386C11D3/38681C12N9/54
    • An alkaline protease variant derived from an alkaline protease consisting of an amino acid sequence represented by SEQ ID NO: 2 or consisting of an amino acid sequence having an identity of 90% or more therewith, which variant has mutations wherein one or more amino acid residues at positions selected from (a) position 6, (b) position 15, (c) position 16, (d) position 65, (e) position 66, (f) position 82, (g) position 83, (h) position 204, (i) position 319, and (j) position 337 of the amino acid sequence represented by SEQ ID NO: 2, or at positions corresponding thereto are substituted with the following amino acid residues: (a) or a position corresponding thereto: Typ, Leu, Val, Ile, Met, Tyr, Gln, Lys, Thr, Phe, Arg, Ser, Cys, Ala, or His; (b) or a position corresponding thereto: Glu, Met, Asp, Val, Gln, Arg, Cys, Trp, Ala, or Phe; (c) or a position corresponding thereto: Met, Glu, Arg, Val, Lys, Phe, Tyr, Ile, His, Asp, or Cys; (d) or a position corresponding thereto: Trp; (e) or a position corresponding thereto: His, Trp, Ser, or Leu; (f) or a position corresponding thereto: Ala, Glu, Gln, Ser, Cys, Gly, His, Lys, Arg, Met, or Asn; (g) or a position corresponding thereto: Ala, Ser, or Cys; (h) or a position corresponding thereto: Glu, Asp, Cys, Val, Thr, Pro, His, Ile, Trp, Ser, Asn, Lys, or Arg; (i) or a position corresponding thereto: Trp, Val, Thr, Leu, Ile, Cys, Glu, Lys, Tyr, Arg, Phe, Gln, Met, Pro, Asp, Asn, His, or Ser; and (j) or a position corresponding thereto: Arg, Gly, Ser, Lys, Gln, Thr, His, Ala, Cys, or Val.
    • 由碱性蛋白酶衍生的碱性蛋白酶变体,其由SEQ ID NO:2所示的氨基酸序列组成或由与其同一性为90%以上的氨基酸序列组成,所述变体具有突变,其中一个或多个氨基酸残基 在从(a)位置6,(b)位置15,(c)位置16,(d)位置65,(e)位置66,(f)位置82,(g)位置83,(h) 204,(i)位置319和(j)由SEQ ID NO:2表示的氨基酸序列的位置337或与其相对应的位置被以下氨基酸残基取代:(a)或与其对应的位置: Typ,Leu,Val,Ile,Met,Tyr,Gln,Lys,Thr,Phe,Arg,Ser,Cys,Ala或His; (b)或相应位置:Glu,Met,Asp,Val,Gln,Arg,Cys,Trp,Ala或Phe; (c)或对应的位置:Met,Glu,Arg,Val,Lys,Phe,Tyr,Ile,His,Asp或Cys; (d)或与之对应的位置:Trp; (e)或与之对应的位置:His,Trp,Ser或Leu; (f)或对应的位置:Ala,Glu,Gln,Ser,Cys,Gly,His,Lys,Arg,Met或Asn; (g)或对应的位置:Ala,Ser或Cys; (h)或相应位置:Glu,Asp,Cys,Val,Thr,Pro,His,Ile,Trp,Ser,Asn,Lys或Arg; (i)或对应的位置:Trp,Val,Thr,Leu,Ile,Cys,Glu,Lys,Tyr,Arg,Phe,Gln,Met,Pro,Asp,Asn,His或Ser; 和(j)或对应的位置:Arg,Gly,Ser,Lys,Gln,Thr,His,Ala,Cys或Val。