专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明授权

US09169512B2 Methods and compositions for nucleic acid amplification 有权
标题翻译：用于核酸扩增的方法和组合物
公开(公告)号：US09169512B2
公开(公告)日：2015-10-27
申请号：US13380018
申请日：2010-07-01
申请人： Steven T. Brentano , Dmitry Lyakhov , James D. Carlson , Michael M. Becker , Norman C. Nelson , Lyle J. Arnold, Jr.
发明人： Steven T. Brentano , Dmitry Lyakhov , James D. Carlson , Michael M. Becker , Norman C. Nelson , Lyle J. Arnold, Jr.
IPC分类号： C12P19/34 , C12Q1/68
CPC分类号： C12Q1/6865 , C12Q1/6844 , C12Q1/6848 , C12Q1/6853 , C12Q2549/119 , C12Q2537/143 , C12Q2531/143
摘要： Compositions, reaction mixtures, and methods for performing an amplification reaction, including multiplex amplification reaction, wherein the method comprises using one or more amplification oligomer complexes comprising linked first and second amplification oligomer members. In one aspect, the amplification oligomer complex is hybridized to a target nucleic acid, the target nucleic acid with hybridized amplification oligomer complex is then captured, and other components are washed away. Target sequences of the target nucleic acids are pre-amplified to generate a first amplification product. The first amplification product is amplified in one or more secondary amplification reactions to generate second amplification products.
摘要翻译：用于进行扩增反应的组合物，反应混合物和方法，包括多重扩增反应，其中所述方法包括使用包含连接的第一和第二扩增寡聚体成员的一种或多种扩增寡聚体复合物。一方面，将扩增寡聚物复合物与靶核酸杂交，然后捕获具有杂交扩增寡聚物复合物的靶核酸，并洗涤其它组分。靶核酸的靶序列被预扩增以产生第一扩增产物。第一扩增产物在一个或多个二级扩增反应中扩增以产生第二扩增产物。

2. 发明授权

US08642268B2 Methods and compositions for nucleic acid amplification 有权
标题翻译：用于核酸扩增的方法和组合物
公开(公告)号：US08642268B2
公开(公告)日：2014-02-04
申请号：US13460341
申请日：2012-04-30
申请人： Steven T. Brentano , Dmitry Lyakhov , James D. Carlson , Norman C. Nelson , Lyle J. Arnold, Jr. , Michael M. Becker
发明人： Steven T. Brentano , Dmitry Lyakhov , James D. Carlson , Norman C. Nelson , Lyle J. Arnold, Jr. , Michael M. Becker
IPC分类号： C12Q1/68 , C12P19/34 , C07H19/00
CPC分类号： C12Q1/6881 , C12Q1/6834 , C12Q1/6844 , C12Q1/6865 , C12Q2525/155 , C12Q2525/186 , C12Q2525/197 , C12Q2531/143 , C12Q2565/543
摘要： Compositions that are used in nucleic acid amplification in vitro are disclosed, which include a target specific universal (TSU) promoter primer or promoter provider oligonucleotide that includes a target specific (TS) sequence that hybridizes specifically to a target sequence that is amplified and a universal (U) sequence that is introduced into the sequence that is amplified, by using a primer for the universal sequence. Methods of nucleic acid amplification in vitro are disclosed that use one or more TSU oligonucleotides to attached a U sequence to a target nucleic acid in a target capture step and then use a primer for a U sequence in subsequent amplification steps performed in substantially isothermal conditions to make amplification products that contain a U sequence that indicates the presence of the target nucleic acid in a sample.
摘要翻译：公开了用于体外核酸扩增的组合物，其包括目标特异性通用（TSU）启动子引物或启动子提供者寡核苷酸，其包括与扩增的靶序列特异性杂交的靶特异性（TS）序列和通用（U）序列，通过使用通用序列的引物引入扩增的序列。公开了体外核酸扩增方法，其中使用一种或多种TSU寡核苷酸将U序列连接到目标捕获步骤中的靶核酸，然后在基本上等温条件下进行的随后扩增步骤中使用U序列引物制备含有指示样品中靶核酸存在的U序列的扩增产物。

3. 发明授权

US08368882B2 Systems and methods for detecting a signal and applying thermal energy to a signal transmission element 有权
标题翻译：用于检测信号并向信号传输元件施加热能的系统和方法
公开(公告)号：US08368882B2
公开(公告)日：2013-02-05
申请号：US12696406
申请日：2010-01-29
申请人： David Opalsky , George T. Walker , Norman C. Nelson , Richard S. Lee , Sara H. Fan
发明人： David Opalsky , George T. Walker , Norman C. Nelson , Richard S. Lee , Sara H. Fan
IPC分类号： G01N21/01
CPC分类号： G01N21/03 , B01L3/5027 , B01L3/505 , B01L7/52 , B01L9/527 , B01L2200/04 , B01L2200/146 , B01L2300/021 , B01L2300/044 , B01L2300/0654 , B01L2300/0672 , B01L2300/0816 , B01L2300/087 , B01L2300/1827 , B01L2400/043 , B01L2400/0478 , B01L2400/0481 , B01L2400/0487 , B01L2400/0605 , B01L2400/0666 , G01N21/0332 , G01N2021/0328 , G01N2021/0346 , G01N2035/00346
摘要： A signal detection system configured for detecting a signal emitted by the contents of a reaction receptacle is also configured to apply thermal energy to a portion of the reaction receptacle to affect a reaction occurring within the reaction receptacle. More particularly, a system for detecting electromagnetic radiation emitted by the contents of a reaction receptacle includes a transmission element configured for transmitting electromagnetic radiation from the contents of the receptacle, a thermal element associated with the transmission element and configured to apply thermal energy to at least a portion of the receptacle, and a detector configured to receive electromagnetic radiation from the transmission element and to generate a signal corresponding to a characteristic of the electromagnetic radiation received by the detector.
摘要翻译：被配置为检测由反应容器的内容物发射的信号的信号检测系统还被配置为将热能施加到反应容器的一部分以影响在反应容器内发生的反应。更具体地，用于检测由反应容器的内容物发射的电磁辐射的系统包括传输元件，其被配置用于从容器的内容物传输电磁辐射，与传输元件相关联的热元件，并被配置为将热能施加到至少所述插座的一部分以及被配置为从所述传输元件接收电磁辐射并且产生对应于由所述检测器接收的电磁辐射的特性的信号的检测器。

4. 发明申请

US20090142771A1 Methods and Instruments for Processing a Sample in a Multi-Chambered Receptacle 有权
标题翻译：用于在多声道插座中处理样品的方法和仪器
公开(公告)号：US20090142771A1
公开(公告)日：2009-06-04
申请号：US12333334
申请日：2008-12-12
申请人： Scott S. Breidenthal , Sara H. Fan , Richard S. Lee , Norman C. Nelson , Matthew J. Scott , Jason A. Taylor
发明人： Scott S. Breidenthal , Sara H. Fan , Richard S. Lee , Norman C. Nelson , Matthew J. Scott , Jason A. Taylor
IPC分类号： C12Q1/68 , G01N33/00 , C12M1/00
CPC分类号： B01F11/00 , B01F3/1271 , B01F11/0045 , B01F11/0071 , B01F13/0059 , B01F15/00214 , B01F2215/0037 , B01L3/502 , B01L3/502715 , B01L3/50273 , B01L3/502761 , B01L7/52 , B01L7/525 , B01L2200/0621 , B01L2200/0647 , B01L2200/0673 , B01L2200/0689 , B01L2200/10 , B01L2200/14 , B01L2200/16 , B01L2300/0816 , B01L2300/087 , B01L2300/0887 , B01L2300/123 , B01L2300/1805 , B01L2300/1822 , B01L2300/1883 , B01L2400/0457 , B01L2400/0478 , B01L2400/0481 , B01L2400/0655 , B01L2400/0677 , C12N15/1006 , C12Q1/6806 , C12Q1/6844 , G01N1/44 , G01N21/01 , G01N21/6428 , G01N21/645 , G01N35/0098 , G01N2021/6419 , G01N2021/6421 , G01N2021/6432 , G01N2021/6439 , G01N2021/6463 , G01N2035/00544 , G01N2201/0627 , Y10T137/0391 , Y10T436/143333 , Y10T436/25 , Y10T436/255
摘要： A receptacle having a plurality of interconnected chambers arranged to permit multiple process steps or processes to be performed independently or simultaneously. The receptacles are manufactured to separate liquid from dried reagents and to maintain the stability of the dried reagents. An immiscible liquid, such as an oil, is included to control loading of process materials, facilitate mixing and reconstitution of dried reagents, limit evaporation, control heating of reaction materials, concentrate solid support materials to prevent clogging of fluid connections, provide minimum volumes for fluid transfers, and to prevent process materials from sticking to chamber surfaces. The receptacles can be adapted for use in systems having a processing instrument that includes an actuator system for selectively moving fluid substances between chambers and a detector. The actuator system can be arranged to concentrate an analyte present in a sample. The detector can be used to detect an optical signal emitted by the contents of the receptacle.
摘要翻译：具有多个互连室的容器，其布置成允许独立地或同时地执行多个工艺步骤或工艺。制造容器以将液体与干燥试剂分开并保持干燥试剂的稳定性。包括不混溶的液体，如油，以控制加工材料的加载，促进干燥试剂的混合和重构，限制蒸发，控制反应材料的加热，浓缩固体支持材料以防止流体连接堵塞，为流体转移，并防止工艺材料粘附到室表面。插座可以适用于具有处理器具的系统，该处理器具包括用于选择性地移动腔室和检测器之间的流体物质的致动器系统。致动器系统可以被布置成集中存在于样品中的分析物。检测器可用于检测由容器的内容物发射的光信号。

5. 发明申请

US20080305482A1 METHODS AND COMPOSITIONS FOR NUCLEIC ACID AMPLIFICATION 有权
标题翻译：用于核酸扩增的方法和组合物
公开(公告)号：US20080305482A1
公开(公告)日：2008-12-11
申请号：US11962072
申请日：2007-12-20
申请人： Steven T. BRENTANO , Dmitry LYAKHOV , James D. CARLSON , Norman C. NELSON
发明人： Steven T. BRENTANO , Dmitry LYAKHOV , James D. CARLSON , Norman C. NELSON
IPC分类号： C12Q1/68 , C07H21/04 , C12P19/34
CPC分类号： C12Q1/6881 , C12Q1/6834 , C12Q1/6844 , C12Q1/6865 , C12Q2525/155 , C12Q2525/186 , C12Q2525/197 , C12Q2531/143 , C12Q2565/543
摘要： Compositions that are used in nucleic acid amplification in vitro are disclosed, which include a target specific universal (TSU) promoter primer or promoter provider oligonucleotide that includes a target specific (TS) sequence that hybridizes specifically to a target sequence that is amplified and a universal (U) sequence that is introduced into the sequence that is amplified, by using a primer for the universal sequence. Methods of nucleic acid amplification in vitro are disclosed that use one or more TSU oligonucleotides to attached a U sequence to a target nucleic acid in a target capture step and then use a primer for a U sequence in subsequent amplification steps performed in substantially isothermal conditions to make amplification products that contain a U sequence that indicates the presence of the target nucleic acid in a sample.
摘要翻译：公开了用于体外核酸扩增的组合物，其包括目标特异性通用（TSU）启动子引物或启动子提供者寡核苷酸，其包括与扩增的靶序列特异性杂交的靶特异性（TS）序列和通用（U）序列，通过使用通用序列的引物引入扩增的序列。公开了体外核酸扩增方法，其中使用一种或多种TSU寡核苷酸将U序列连接到目标捕获步骤中的靶核酸，然后在基本上等温条件下进行的随后扩增步骤中使用U序列引物制备含有指示样品中靶核酸存在的U序列的扩增产物。

6. 发明授权

US5639604A Homogeneous protection assay 失效
标题翻译：均质保护试验
公开(公告)号：US5639604A
公开(公告)日：1997-06-17
申请号：US161706
申请日：1993-12-03
申请人： Lyle J. Arnold, Jr. , Norman C. Nelson
发明人： Lyle J. Arnold, Jr. , Norman C. Nelson
IPC分类号： C12Q1/68 , G01N33/53 , G01N33/532 , G01N33/533 , G01N33/542 , G01N33/58 , C07H21/04 , C12Q1/00
CPC分类号： G01N33/58 , C12Q1/6816 , C12Q1/6823 , G01N33/5306 , G01N33/532 , G01N33/533 , G01N33/542
摘要： Improved homogenous diagnostic assay methods and labels for detecting an analyte in a medium when the analyte is a member of a specific binding pair. The methods and labels provide procedures for reducing background and increasing sensitivity. The binding partner of the analyte is labeled with a substance, the stability of which detectably changes whenever said analyte is bound as a member of the specific binding pair. In a closely related system, the analyte is labeled with a substance susceptible to differential degradation depending on whether or not the analyte is bound as a member of its specific binding pair. After incubation and selective degradation or chemical or biochemical alteration, the amount of analyte bound is detected by measuring either the stability change or the extent of degradation of the label. In a particular system, chemiluminescent acridinium ester labeled probes are used in a homogenous hybridization assay format for sensitively detecting the presence of complement any target polynucleotide sequences.
摘要翻译：当分析物是特异性结合对的成员时，改进的同源诊断测定方法和用于检测培养基中分析物的标记。方法和标签提供了降低背景和提高灵敏度的程序。分析物的结合配偶体用物质标记，当所述分析物作为特异性结合对的成员结合时，其稳定性可检测地改变。在紧密相关的系统中，根据分析物是否作为其特异性结合对的成员结合，分析物被易受差异降解敏感的物质标记。孵育和选择性降解或化学或生物化学改变后，通过测量标签的稳定性变化或降解程度来检测分析物的结合量。在特定系统中，化学发光的吖啶酯标记的探针以均质杂交测定形式使用，以敏感地检测补体存在任何靶多核苷酸序列。

7. 发明授权

US5185439A Acridinium ester labelling and purification of nucleotide probes 失效
标题翻译：吖啶酯标记和纯化核苷酸探针
公开(公告)号：US5185439A
公开(公告)日：1993-02-09
申请号：US332939
申请日：1988-12-12
申请人： Lyle J. Arnold, Jr. , Norman C. Nelson
发明人： Lyle J. Arnold, Jr. , Norman C. Nelson
IPC分类号： C07H21/00
CPC分类号： C07H21/00
摘要： Methods for the construction, labelling and subsequent purification of nucleic acid probes containing primary amines with acridinium esters 4-(2-succinimidyloxycarbonyl-ethyl)phenyl-10-methylacridinium-9-carboxylate fluorosulfonate). The method for attaching acridinium esters to probes uses high (0.1 to 50 mM) acridinium ester concentrations achieved using organic solvent in concentrations of 20% to 80% by volume, and may be carried out either in solution, or with one or the other of the acridinium ester or the probe suspended in solution. Purification (the separation of labelled probe from unlabelled probe and free label) involves (1) first removing most of the free acridinium ester label from probe using rapid separation techniques, then (2) removing substantially all remaining free label from the probe and separating labelled probe from unlabelled probe, involves specific applications of ion exchange, reverse phase or hydroxyapatite HPLC.
摘要翻译：含有伯胺与吖啶酯4-（2-琥珀酰亚氨基氧羰基 - 乙基）苯基-10-甲基吖啶-9-甲酸氟代磺酸酯的核酸探针的构建，标记和随后纯化的方法。将吖啶酯连接到探针的方法使用浓度为20％至80％（体积）的有机溶剂获得的高（0.1至50mM）吖啶酯浓度，并且可以在溶液中或与其中的一种或一种吖啶酯或探针悬浮在溶液中。纯化（标记的探针与未标记的探针和游离标记物的分离）包括：（1）首先使用快速分离技术从探针中除去大部分游离的吖啶酯标记物，然后（2）从探针中基本上除去所有剩余的游离标记并分离标记来自未标记探针的探针涉及离子交换，反相或羟基磷灰石HPLC的具体应用。

8. 发明授权

US4581686A Rotating head switch mechanism for flashlight 失效
标题翻译：手电筒旋转头开关机构
公开(公告)号：US4581686A
公开(公告)日：1986-04-08
申请号：US650116
申请日：1984-09-13
申请人： Norman C. Nelson
发明人： Norman C. Nelson
IPC分类号： F21V23/00 , F21V23/04 , F21W111/10 , F21L9/00
CPC分类号： F21L15/06 , F21L4/005 , F21V23/0414 , F21V23/0428
摘要： The present invention relates to a flashlight assembly having a rotatable head which acts as a switch mechanism for turning the light on or off.The flashlight has an elongated metal housing, and the rotatable head is also made of metal. A spacer member made of an electrical insulating material is securely attached to the head member. The spacer member threadedly engages threads formed on the housing. Thus, there is normally no electrical continuity between the metal head member and the metal housing. However, when the head member is rotated in a tightening direction, a flat annular face on the head member comes into mating engagement with a similar face on the housing, thus establishing electrical continuity between those two members and completing the internal electrical circuit of the flashlight.
摘要翻译：本发明涉及一种手电筒组件，其具有旋转头部，其作为用于打开或关闭灯的开关机构。手电筒具有细长的金属外壳，旋转头也由金属制成。由电绝缘材料制成的间隔件牢固地附接到头部构件。间隔构件螺纹接合形成在壳体上的螺纹。因此，金属头部件和金属外壳之间通常不具有电连续性。然而，当头部构件沿紧固方向旋转时，头部构件上的平坦环形面与壳体上的类似面部配合接合，从而在这两个构件之间建立电连续性并完成手电筒的内部电路。

9. 发明授权

US4357648A Rechargeable flashlight 失效
标题翻译：充电手电筒
公开(公告)号：US4357648A
公开(公告)日：1982-11-02
申请号：US237451
申请日：1981-02-23
申请人： Norman C. Nelson
发明人： Norman C. Nelson
IPC分类号： F21L4/08 , F21L7/00
CPC分类号： F21V19/047 , F21L4/085
摘要： A flashlight is equipped with rechargeable batteries inside its main housing. A removable tail cap contains an electronic circuit for recharging the batteries, the circuit being actuated and energized by means of a switch assembly which is accessible through the outer end of the tail cap.A mechanical isolation means is included for protecting the electronic circuit from being damaged by any physical movement of the batteries that may occur.One embodiment of the invention also includes a spare bulb holder assembly which also constitutes the mechanical isolation means.
摘要翻译：手电筒内部装有充电电池。可移除的尾盖包含用于对电池充电的电子电路，电路通过可通过尾盖的外端接近的开关组件致动和激励。包括用于保护电子电路不会被可能发生的电池的任何物理运动损坏的机械隔离装置。本发明的一个实施例还包括也构成机械隔离装置的备用灯泡保持器组件。

10. 发明授权

US4037839A Collapsible baton 失效
标题翻译：可折叠指挥棒
公开(公告)号：US4037839A
公开(公告)日：1977-07-26
申请号：US645667
申请日：1975-12-31
申请人： Norman C. Nelson
发明人： Norman C. Nelson
IPC分类号： F41B15/02
CPC分类号： F41B15/027 , F41B15/025 , Y10T403/32483 , Y10T403/32991 , Y10T403/592
摘要： A police baton or the like of telescoping tubular sections normally carried in a collapsed telescoped condition and extensible by releasing a safety lock in the handle and exerting pressure upon an eject button to release a catch and permit an internal expansive helical spring to telescopically extend the sections of the baton, operation of the safety lock and of the eject button being accomplished by movement of those elements in readily distinguishable directions of operational motion.
摘要翻译：伸缩管状部件的警棍或类似物通常以折叠的伸缩状态运载，并通过释放手柄中的安全锁并在弹出按钮上施加压力以释放卡扣并允许内部膨胀螺旋弹簧将部分的指挥棒，安全锁的操作和弹出按钮的操作通过这些元件在操作运动的容易区分的方向上的移动来实现。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式