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1. 发明申请

WO2011025819A1 DETECTION AND QUANTIFICATION OF HYDROXYMETHYLATED NUCLEOTIDES IN A POLYNUCLEOTIDE PREPARATION 审中-公开
标题翻译：羟基甲基纤维素在多核苷酸制备中的检测和定量
公开(公告)号：WO2011025819A1
公开(公告)日：2011-03-03
申请号：PCT/US2010/046632
申请日：2010-08-25
申请人： NEW ENGLAND BIOLABS, INC. , BITINAITE, Jurate , VAISVILA, Romaldas , PRADHAN, Sriharsa , ZHENG, Yu , ROBERTS, Richard, J. , CHIN, Hang-gyeong , COHEN-KARNI, Devora , NOREN, Christopher , RALEIGH, Elisabeth, A.
发明人： BITINAITE, Jurate , VAISVILA, Romaldas , PRADHAN, Sriharsa , ZHENG, Yu , ROBERTS, Richard, J. , CHIN, Hang-gyeong , COHEN-KARNI, Devora , NOREN, Christopher , RALEIGH, Elisabeth, A.
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6883 , C12Q1/6827 , C12Q2600/154 , C12Q2521/331 , C12Q2525/191 , C12Q2531/113
摘要： Methods and compositions are described for detecting hydroxymethylated nucleotides (hmNs) in a polynucleotide preparation with a view to mapping the location of hmNs in a genome, quantifying the occurrence of hmNs at selected loci and correlating the occurrence of hmNs with gene expression and phenotypic traits. Embodiments describe the use of modifying enzymes together with site-specific endonucleases to detect the hmNs.
摘要翻译：描述了用于检测多核苷酸制备物中的羟甲基化核苷酸（hmN）的方法和组合物，以便映射基因组中hmN的位置，定量选择的位点的hmN的发生，并将hmNs的发生与基因表达和表型性状相关联。实施方案描述了使用将酶修饰酶与位点特异性内切核酸酶一起检测hmN。

2. 发明申请

WO1997001642A1 MODIFIED PROTEINS AND METHODS OF THEIR PRODUCTION 审中-公开
标题翻译：改良蛋白质及其生产方法
公开(公告)号：WO1997001642A1
公开(公告)日：1997-01-16
申请号：PCT/US1996010545
申请日：1996-06-19
申请人： NEW ENGLAND BIOLABS, INC. , COMB, Donald, G. , PERLER, Francine, B. , JACK, William, E. , XU, Ming-Qun , HODGES, Robert, A. , NOREN, Christopher, J. , CHONG, Shaorong, S., C.
发明人： NEW ENGLAND BIOLABS, INC.
IPC分类号： C12P21/00
CPC分类号： C12Y302/01081 , C07K1/1075 , C07K2319/20 , C07K2319/21 , C07K2319/92 , C07K2319/95 , C12N9/1252 , C12N9/14 , C12N9/22 , C12N9/2442 , C12N9/2471 , C12N15/62 , C12P21/02 , C12Y302/01014 , C12Y302/01023
摘要： The present invention is directed to modified proteins and methods of their production. The modified proteins comprise a controllable intervening protein sequence (CIVPS) inserted into a target protein, the CIVPS being capable of excision from or cleavage of the modified protein under predetermined conditions in cis or in trans, i.e., increase in temperature, exposure to light, unblocking of amino acid residues by dephosphorylation, treatment with chemical reagents or deglycosylation. If desired, the modified protein can be subjected to these conditions. The CIVPS may also be inserted into a region that substantially inactivates target protein activity.
摘要翻译：本发明涉及修饰的蛋白质及其生产方法。修饰的蛋白质包含插入到靶蛋白质中的可控中间蛋白质序列（CIVPS），所述CIVPS能够在预定条件下以顺式或反式切割或切割修饰的蛋白质，即温度升高，暴露于光，通过去磷酸化解除氨基酸残基，用化学试剂或去糖基化处理。如果需要，修饰的蛋白质可以经受这些条件。 CIVPS也可以插入基本上灭活靶蛋白活性的区域。

3. 发明申请

WO9911821A9 METHOD FOR SCREENING RESTRICTION ENDONUCLEASES 审中-公开
标题翻译：筛选限制性内切酶的方法
公开(公告)号：WO9911821A9
公开(公告)日：1999-08-05
申请号：PCT/US9818124
申请日：1998-09-01
申请人： NEW ENGLAND BIOLABS INC , ROBERTS RICHARD J , BYRD DEVON R , MORGAN RICHARD D , PATTI JAY , NOREN CHRISTOPHER J
发明人： ROBERTS RICHARD J , BYRD DEVON R , MORGAN RICHARD D , PATTI JAY , NOREN CHRISTOPHER J
IPC分类号： B26D1/00 , B26D1/08 , B42C5/00 , C12N9/22 , C12Q1/68 , C12N1/20 , C12N9/14 , C12N15/00 , C12P21/06
CPC分类号： B26D1/0006 , B26D1/08 , B26D2001/006 , B42C5/00 , C12N9/22 , G01N2333/922
摘要： A method is provided for identifying a restriction endonuclease, which includes the steps of (a) screening a target DNA sequence for the presence of known methylase sequence motifs, (b) identifying any open reading frames which lie close to the methylase sequence motifs screened in step (a), and (c) assaying the protein products of these open reading frames for restriction endonuclease activity. Methods for identifying isoschizomers of known restriction endonucleases, which isoschizomers possess a desired physical property, such as thermostability, are also provided by the present invention, as are several novel restriction endonucleases isolated from M. jannaschii, MjaIII and MjaIV. Additionally, a gene was identified that encoded a previously observed endonuclease activity, designated MjaII. Also provided by the present invention are vectors suitable for cloning a DNA sequence encoding a cytotoxic protein, via independent transcription promotors which may be selectively controlled by several conditions. A method for producing these cytotoxic proteins using such vectors is also provided, as are stable clones of PacI and NlaIII.
摘要翻译：提供了用于鉴定限制性内切核酸酶的方法，其包括以下步骤：（a）筛选靶DNA序列以存在已知的甲基化酶序列基序，（b）鉴定位于接近甲基化酶序列基序的任何开放阅读框，步骤（a）和（c）测定这些开放阅读框的蛋白质产物用于限制性内切核酸酶活性。用于鉴定已知限制性内切核酸酶的异构体的方法，其中同分异构体具有期望的物理性质，例如热稳定性，也由本发明提供，以及从分枝杆菌，MjaIII和MjaIV分离的几种新的限制性内切核酸酶。此外，鉴定了编码先前观察到的内切核酸酶活性的基因，命名为MjaII。本发明还提供了适合于通过独立转录启动子克隆编码细胞毒性蛋白质的DNA序列的载体，其可以通过几种条件选择性地控制。还提供了使用这种载体产生这些细胞毒性蛋白质的方法，以及PacI和NlaIII的稳定克隆。

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