专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

WO2002100998A3 METHOD FOR GENERATING DIVERSITY 审中-公开
公开(公告)号：WO2002100998A3
公开(公告)日：2002-12-19
申请号：PCT/GB2002/002688
申请日：2002-06-11
申请人： MEDICAL RESEARCH COUNCIL , SALE, Julian, Edward , NEUBERGER, Michael, Samuel , CUMBERS, Sarah, Jane
发明人： SALE, Julian, Edward , NEUBERGER, Michael, Samuel , CUMBERS, Sarah, Jane
IPC分类号： A01K67/027
摘要： The invention relates to a method for preparing an antibody-producing cell line capable of directed constitutive hypermutation of a specific nucleic acid region, comprising the steps of: a) screening a clonal cell population for V gene diversity; b)isolating one or more cells which display V gene diversity and comparing the rate of accumulation of mutations in the V genes and other genes of the selected cells; and c) selecting a cell in which the rate of V gene mutation exceeds that of other gene mutation.

2. 发明申请

WO2003095636A2 ACTIVATION INDUCED DEAMINASE (AID) 审中-公开
标题翻译：激活诱导的杀伤细胞（AID）
公开(公告)号：WO2003095636A2
公开(公告)日：2003-11-20
申请号：PCT/GB2003/002002
申请日：2003-05-09
申请人： MEDICAL RESEARCH COUNCIL , PETESERSEN-MAHRT, Svend, K. , HARRIS, Reuben, S. , NEUBERGER, Michael, Samuel , BEALE, Rupert, Christopher, Lansdowne
发明人： PETESERSEN-MAHRT, Svend, K. , HARRIS, Reuben, S. , NEUBERGER, Michael, Samuel , BEALE, Rupert, Christopher, Lansdowne
IPC分类号： C12N9/00
CPC分类号： C12N9/78 , C07K2319/00 , C12N2510/00
摘要： The present invention identifies that the expression of Activation Induced Deaminase (AID) or its homologues in cells confers a mutator phenotype and thus provides a method for generating diversity in a gene or gene product as well as cell lines capable of generating diversity in defined gene products. The invention also provides methods of modulating a mutator phenotype by modulating AID expression or activity.
摘要翻译：本发明鉴定了激活诱导的脱氨酶（AID）或其同源物在细胞中的表达赋予突变体表型，从而提供了在基因或基因产物以及能够产生定义的基因产物多样性的细胞系中产生多样性的方法。本发明还提供了通过调节AID表达或活性来调节变异体表型的方法。

3. 发明申请

WO1990004036A1 PRODUCTION OF ANTIBODIES FROM TRANSGENIC ANIMALS 审中-公开
标题翻译：从转基因动物生产抗体
公开(公告)号：WO1990004036A1
公开(公告)日：1990-04-19
申请号：PCT/GB1989001207
申请日：1989-10-12
申请人： MEDICAL RESEARCH COUNCIL , AGRICULTURE AND FOOD RESEARCH COUNCIL , BRUGGEMANN, Marianne , SURANI, Azim, M. , NEUBERGER, Michael, Samuel
发明人： MEDICAL RESEARCH COUNCIL , AGRICULTURE AND FOOD RESEARCH COUNCIL
IPC分类号： C12P21/08
CPC分类号： C12N15/8509 , A01K67/0278 , A01K2207/15 , A01K2217/00 , A01K2217/05 , A01K2227/105 , A01K2267/01 , C07K16/461 , C07K2317/24 , C07K2319/00
摘要： Chimaeric or wholly foreign immunoglobulin is obtained from cells or body fluid of a transgenic animal which has had inserted into its germline genetic material that encodes for at least part of an immunoglobulin of foreign origin or that can rearrange to encode a repertoire of immunoglobulins, i.e. derived from a different animal source. For example, wholly human immunoglobulins may be produced from a transgenic mouse, possibly in response to an immunogen subsequently introduced to the mouse.
摘要翻译：嵌合或完全外源的免疫球蛋白是从已经插入其编码至少部分外源免疫球蛋白的种系遗传物质的转基因动物的细胞或体液中获得的，或者可重排编码免疫球蛋白的所有组成成分，即衍生来自不同的动物来源。例如，完全人免疫球蛋白可以从转基因小鼠产生，可能是随后随后引入小鼠的免疫原。

4. 发明申请

WO1986001533A1 PRODUCTION OF CHIMERIC ANTIBODIES 审中-公开
标题翻译：生产阴茎抗体
公开(公告)号：WO1986001533A1
公开(公告)日：1986-03-13
申请号：PCT/GB1985000392
申请日：1985-09-03
申请人： CELLTECH LIMITED , NEUBERGER, Michael, Samuel , RABBITTS, Terence, Howard
发明人： CELLTECH LIMITED
IPC分类号： C12N15/00
CPC分类号： C07K16/44 , C07K16/462 , C07K2317/24 , C07K2319/00 , C07K2319/30 , G01N33/531 , G01N33/563
摘要： A process for the production of a chimeric antibody, comprising: a) preparing a replicable expression vector including a suitable promoter operably linked to a DNA sequence comprising a first part which encodes at least the variable region of the heavy or light chain of an Ig molecule and a second part which encodes at least part of a second protein; b) if necessary, preparing a replicable expression vector including a suitable promoter operably linked to a DNA sequence which encodes at least the variable region of a complementary light or heavy chain respectively of an Ig molecule; c) transforming an immortalised mammalian cell line with the or both prepared vectors; and d) culturing said transformed cell line to produce the chimeric antibody; chimeric antibodies produced by this process; and plasmids and transformed cell lines used in the process.

5. 发明申请

WO02100998A2 METHOD FOR GENERATING DIVERSITY 审中-公开
标题翻译：生成多样性的方法
公开(公告)号：WO02100998A2
公开(公告)日：2002-12-19
申请号：PCT/GB0202688
申请日：2002-06-11
申请人： MEDICAL RES COUNCIL , SALE JULIAN EDWARD , NEUBERGER MICHAEL SAMUEL , CUMBERS SARAH JANE
发明人： SALE JULIAN EDWARD , NEUBERGER MICHAEL SAMUEL , CUMBERS SARAH JANE
IPC分类号： C12N15/00 , A01K67/027 , C07K16/00 , C12N5/08 , C12N5/10 , C12N9/00 , C12N15/09 , C12N15/10 , C12P21/02 , C12G1/68
CPC分类号： C12N15/01 , A01K67/0275 , A01K2217/05 , C07K16/00 , C07K2317/56 , C12N15/1024 , C12N15/1075
摘要： The invention relates to a method for preparing an antibody-producing cell line capable of directed constitutive hypermutation of a specific nucleic acid region, comprising the steps of: a) screening a clonal cell population for V gene diversity; b)isolating one or more cells which display V gene diversity and comparing the rate of accumulation of mutations in the V genes and other genes of the selected cells; and c) selecting a cell in which the rate of V gene mutation exceeds that of other gene mutation.
摘要翻译：本发明涉及一种制备能够引导特异性核酸区域的组成型突变的产生抗体的细胞系的方法，包括以下步骤：a）筛选V基因多样性的克隆细胞群; b）分离显示V基因多样性的一个或多个细胞，并比较V基因和所选细胞的其他基因中突变的积累速率; c）选择V基因突变率超过其他基因突变的细胞。

6. 发明申请

WO1989001975A1 RECOMBINANT ANTIBODIES AND METHODS FOR THEIR PRODUCTION 审中-公开
标题翻译：重组抗体及其生产方法
公开(公告)号：WO1989001975A1
公开(公告)日：1989-03-09
申请号：PCT/GB1988000695
申请日：1988-08-24
申请人： CELLTECH LIMITED , CATTANEO, Antonino , NEUBERGER, Michael, Samuel
发明人： CELLTECH LIMITED
IPC分类号： C12P21/00
CPC分类号： C07K16/00 , A61K38/00 , C07K16/40
摘要： There is provided a process for the production of an assembled polymeric recombinant antibody having its constant regions derived from an IgM, characterised in that the process is carried out in the absence of J-chain or any active gene coding therefor. Preferably, the host cell is an actively secreting cell, such as a glial cell, so that the transformed cell can be transplanted into the brain.
摘要翻译：提供了一种制备其具有源自IgM的恒定区的组装的聚合物重组抗体的方法，其特征在于该方法在不存在J链或任何其编码的活性基因的情况下进行。优选地，宿主细胞是主动分泌的细胞，例如胶质细胞，使得转化的细胞可以移植到脑中。

7. 发明申请

WO2003095636A3 ACTIVATION INDUCED DEAMINASE (AID) 审中-公开
公开(公告)号：WO2003095636A3
公开(公告)日：2003-11-20
申请号：PCT/GB2003/002002
申请日：2003-05-09
申请人： MEDICAL RESEARCH COUNCIL , PETESERSEN-MAHRT, Svend, K. , HARRIS, Reuben, S. , NEUBERGER, Michael, Samuel , BEALE, Rupert, Christopher, Lansdowne
发明人： PETESERSEN-MAHRT, Svend, K. , HARRIS, Reuben, S. , NEUBERGER, Michael, Samuel , BEALE, Rupert, Christopher, Lansdowne
IPC分类号： C12N1/21
摘要： The present invention identifies that the expression of Activation Induced Deaminase (AID) or its homologues in cells confers a mutator phenotype and thus provides a method for generating diversity in a gene or gene product as well as cell lines capable of generating diversity in defined gene products. The invention also provides methods of modulating a mutator phenotype by modulating AID expression or activity.

8. 发明申请

WO1990012878A1 IMPROVEMENTS IN OR RELATING TO ENHANCERS 审中-公开
标题翻译：改善或与增强器相关的改进
公开(公告)号：WO1990012878A1
公开(公告)日：1990-11-01
申请号：PCT/GB1990000604
申请日：1990-04-20
申请人： MEDICAL RESEARCH COUNCIL , NEUBERGER, Michael, Samuel , MEYER, Kerstin, Barbara
发明人： MEDICAL RESEARCH COUNCIL
IPC分类号： C12N15/63
CPC分类号： C12N15/85 , A61K48/00 , C12N2830/30 , C12N2830/60
摘要： A novel enhancer, known as the K3'-enhancer, has been identified in the mouse immunoglobulin K locus, downstream of CK. The K3'-enhancer is B cell specific, is some sevenfold stronger than the K-intron enhancer and the region of the enhancer shows striking sequence homologies to the lymphotopic papovavirus, IgH and K-intron enhancers. The novel enhancer allows high level expression both in transfected cell lines and in transgenic mice, and may thus be used to enhance expression of genes in host cells, in vivo and in vitro. The novel enhancer is also found to increase somatic mutation of antibody genes in transgenic mice. The novel enhancer can also be used in gene therapy. Functionally equivalent enhancers are to be expected to occur downstream of CK of the immunoglobulin K locus of other species.
摘要翻译：已经在CK的下游的小鼠免疫球蛋白K基因座中鉴定了一种称为K3增强子的新型增强子。 K3'增强子是B细胞特异性的，比K-内含子增强子强7倍，增强子区显示与淋巴细胞乳头状瘤病毒，IgH和K-内含子增强子具有惊人的序列同源性。新型增强子允许在转染的细胞系和转基因小鼠中进行高水平表达，因此可用于体内和体外增强宿主细胞中基因的表达。还发现新型增强子增加转基因小鼠中抗体基因的体细胞突变。该新型增强子也可用于基因治疗。预期功能上等同的增强剂将发生在其他物种的免疫球蛋白K基因座的CK下游。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式