专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明专利

JP2010181391A Identifying method of animal cell 审中-公开
标题翻译：动物细胞鉴定方法
公开(公告)号：JP2010181391A
公开(公告)日：2010-08-19
申请号：JP2009049889
申请日：2009-02-05
申请人： Mutsumi Takagi , 睦高木
发明人： TAKAGI MUTSUMI
IPC分类号： G01N21/65 , C12Q1/04 , G01N33/48
摘要： PROBLEM TO BE SOLVED: To provide a method of nondestructively and non-invasively identifying the type and specialization of a cell under incubation based on the variation of chemical composition of the cell associated with difference and specialization of cell species when the animal cell is incubated for transplantation.
SOLUTION: The type and specialization of the cell under incubation can be nondestructively and non-invasively identified based on the chemical composition analysis of the cell by a Raman scattering spectrum when the animal cell is incubated.
COPYRIGHT: (C)2010,JPO&INPIT
摘要翻译：要解决的问题：提供一种非侵入性和非侵入性地鉴定孵育期间细胞的类型和专业化的方法，其基于与动物细胞的细胞种类的差异和特异性相关的细胞的化学组成的变化孵育用于移植。解决方案：孵化期间细胞的类型和专业化可以在动物细胞孵育时，通过拉曼散射光谱，基于细胞的化学成分分析，进行非破坏性和非侵入性鉴定。版权所有（C）2010，JPO＆INPIT

2. 发明专利

JP2006115798A Automatic cell-culturing device by utilizing autoclave disinfection and method for using the same 有权
标题翻译：自动细胞培养装置利用自动消毒及其使用方法
公开(公告)号：JP2006115798A
公开(公告)日：2006-05-11
申请号：JP2004309295
申请日：2004-10-25
申请人： Kawasaki Heavy Ind Ltd , Mutsumi Takagi , 川崎重工業株式会社 , 睦高木
发明人： TAKAGI MUTSUMI , NAKAJIMA KATSUMI , DOI TOSHINAO , OGATA TAKAMASA
IPC分类号： C12M1/00 , C12M3/00 , C12N5/06
CPC分类号： A61L2/07 , C12M23/50 , C12M37/00 , C12M41/48 , Y10S435/809
摘要： PROBLEM TO BE SOLVED: To provide an automatic cell-culturing device without generating cross-contamination even on using the cell cultures of a plurality of subjects and also capable of disinfecting including a frozen storage part and normal temperature storage part. SOLUTION: This automatic cell-culturing device equipped with a culture-operating part necessary for culturing the cells of subjects, an incubator part for the cell culture, a frozen storage part for preserving reagents, a normal temperature storage part of culturing tools, an entrance and exit part of the reagents and culturing tools, and a steam-feeding part for an autoclave disinfection is provided by communicating each of the frozen storage part, normal temperature storage part and entrance/exit part with the operating part, installing each hermetically closing door between these and the operating part, also the hermetically closing door between a filtering part performing the removal of dusts and disinfection of air flow supplied to the operating part and the operating part. By opening/closing the hermetically closing doors selectively, the steam from the steam-supplying part is fed to any of the operating part and the incubator part, frozen storage part, normal temperature storage part and entrance/exit part connected with the operating part. COPYRIGHT: (C)2006,JPO&NCIPI
摘要翻译：要解决的问题：为了提供自动细胞培养装置，即使使用多个受试者的细胞培养物也能产生交叉污染，并且还能够消毒，包括冷藏储存部分和常温储存部分。解决方案：该自动细胞培养装置配备有用于培养受试者细胞所需的培养操作部分，用于细胞培养的培养部分，用于保存试剂的冷冻储存部分，培养工具的常温储存部分，试剂和培养工具的入口和出口部分，以及用于高压灭菌消毒的蒸汽供给部分，通过将每个冷冻储存部分，常温储存部分和入口/出口部分与操作部分连通，将每个在这些与操作部分之间的密封关闭门，还有一个过滤部件执行除尘和消毒供给操作部件和操作部件的空气流的过滤部件之间的气密关闭门。通过选择性地打开/关闭气密关闭门，来自蒸汽供应部分的蒸汽被供给到与操作部分连接的操作部分，孵化器部分，冷藏存储部分，常温储存部分和入口/出口部分中的任何一个。版权所有（C）2006，JPO＆NCIPI

3. 发明专利

JP2005237365A Medium for culturing cell 审中-公开
标题翻译：培养细胞培养基
公开(公告)号：JP2005237365A
公开(公告)日：2005-09-08
申请号：JP2004093750
申请日：2004-02-29
申请人： Mutsumi Takagi , 睦高木
发明人： TAKAGI MUTSUMI
IPC分类号： C12N5/02 , C12N5/07 , C12N5/077 , C12N5/06
摘要： PROBLEM TO BE SOLVED: To provide a medium for culturing cells without risk of contamination of a pathogen to human caused by adding mammal serum such as bovine serum to the medium. SOLUTION: The medium for culturing animal cells contains fish serum instead of mammal serum. The medium for culturing mammal cells contains at least fish serum. COPYRIGHT: (C)2005,JPO&NCIPI
摘要翻译：待解决的问题：提供用于培养细胞的培养基，而不会通过向培养基中加入牛血清等哺乳动物血清而污染人的病原体的风险。解决方案：用于培养动物细胞的培养基含有鱼血清而不是哺乳动物血清。用于培养哺乳动物细胞的培养基至少含有鱼血清。版权所有（C）2005，JPO＆NCIPI

4. 发明专利

JP2004049197A Cell-culturing composition containing synthetic polymer 审中-公开
标题翻译：含有合成聚合物的细胞培养组合物
公开(公告)号：JP2004049197A
公开(公告)日：2004-02-19
申请号：JP2002241203
申请日：2002-07-17
申请人： Mutsumi Takagi , 高木睦
发明人： TAKAGI MUTSUMI , YOSHIDA TOSHIOMI , WAKITANI SHIGEYUKI
IPC分类号： C12N1/00 , C12N5/07 , C12N5/071 , C12N5/06
摘要： PROBLEM TO BE SOLVED: To provide a culturing composition in a three-dimensional cell culture using a synthetic polymer as a scaffold, reducing an inhibitory activity against the culture by decomposed materials of the polymer. SOLUTION: This culture composition is characterized with that the volume of a liquid medium is ≥5 times of a polymer bulk volume, in an animal cell culture consisting of at least a solid synthetic polymer and the liquid medium in addition to the cells. COPYRIGHT: (C)2004,JPO
摘要翻译：待解决的问题：为了提供使用合成聚合物作为支架的三维细胞培养物中的培养组合物，通过聚合物的分解材料降低对培养的抑制活性。解决方案：该培养组合物的特征在于，在至少由固体合成聚合物和液体培养基组成的动物细胞培养物中，除了细胞之外，液体培养基的体积是聚合物体积体积的≥5倍。版权所有（C）2004，JPO

5. 发明专利

JP2014200241A 細胞培養リアクター审中-公开
标题翻译：细胞培养物反应器
公开(公告)号：JP2014200241A
公开(公告)日：2014-10-27
申请号：JP2013088819
申请日：2013-04-02
申请人：高木睦 , Mutsumi Takagi , 睦高木
发明人： TAKAGI MUTSUMI
IPC分类号： C12M3/00
摘要：【課題】本発明は、汚染の防止とともに効率的な体外培養を実現した細胞培養リアクター提供する。【解決手段】移植等の再生治療を目的とした生体由来の細胞の体外培養に関する作業効率や品質管理の向上に関するものであり、マルチスケール培養液量対応、シングルユースで、マイクロキャリアーが付着しにくく、コンタミネーションの可能性がない薬液容器接続が可能なマイクロキャリアー法動物細胞培養リアクターを可能とするものである。さらには、マイクロキャリアーへの接着細胞状態の、コンタミネーションの可能性がないin situ細胞観察を可能とするマイクロキャリアー法動物細胞培養リアクターである。【選択図】図4
摘要翻译：要解决的问题：提供一种在防止污染的同时实现有效的外植体的细胞培养反应器。解决方案：本发明涉及与用于再生医学治疗如移植的来自活生物体的细胞的移植相关的工作效率和质量控制的改进。本发明能够实现准备用于多尺度培养溶液量和一次使用的微载体方法的动物细胞培养反应器，并且微载体难以粘附，并且可以连接到液体药物容器而没有污染的可能性。此外，微载体法动物细胞培养反应器能够在将细胞粘附到微载体的状态下进行原位细胞观察，而不会发生污染。

6. 发明专利

JP2010046053A Sheet-shaped animal cell aggregation-cultured composition and method for making the same 审中-公开
标题翻译：片状动物细胞聚集培养的组合物及其制备方法
公开(公告)号：JP2010046053A
公开(公告)日：2010-03-04
申请号：JP2008244903
申请日：2008-08-19
申请人： Mutsumi Takagi , 睦高木
发明人： TAKAGI MUTSUMI
IPC分类号： C12N5/07 , C12M3/04
摘要： PROBLEM TO BE SOLVED: To provide a sheet-shaped animal cell aggregation-cultured composition having a regulated shape and size without using scaffold when culturing animal cells in vitro. SOLUTION: The sheet-shaped animal cell aggregation-cultured composition is obtained by carrying out centrifugal separation after inserting an animal cell suspension into a container including a flat inner face orthogonal to the radial direction of the centrifugal rotation, and starting the culture in the container while forming the sheet-shaped animal cell aggregation. COPYRIGHT: (C)2010,JPO&INPIT
摘要翻译：待解决的问题：在体外培养动物细胞时，提供具有调节形状和大小的片状动物细胞聚集培养的组合物，而不使用支架。解决方案：通过在将动物细胞悬浮液插入到与离心旋转的径向正交的平坦的内表面的容器中进行离心分离而获得片状动物细胞聚集培养的组合物，并开始培养在容器中形成片状动物细胞聚集体。版权所有（C）2010，JPO＆INPIT

7. 发明专利

JP2011156329A Bone/cartilage-like structure 审中-公开
标题翻译：骨/类似结构
公开(公告)号：JP2011156329A
公开(公告)日：2011-08-18
申请号：JP2010036625
申请日：2010-02-01
申请人： Mutsumi Takagi , 睦高木
发明人： TAKAGI MUTSUMI , WAKITANI SHIGEYUKI
IPC分类号： A61L27/00
摘要： PROBLEM TO BE SOLVED: To provide a bone/cartilage-like structure for transplantation wherein a cartilage-like structure prepared without using scaffold and an artificial bone material are firmly bonded, and a method for making the same. SOLUTION: As a result of keen examinations to solve the problem, the invention is completed. That is, in the invention, a sheet-shaped animal cell aggregation obtained by carrying out centrifugal sedimentation is cultured in a container after inserting an animal cell suspension into the container including a flat inner bottom face orthogonal to the radial direction of the centrifugal rotation so as to carry out centrifugal separation, and after starting the culture, a bone-like solid is placed on the cell aggregation on a bottom surface of the culture container so as to make the bone/cartilage-like structure. COPYRIGHT: (C)2011,JPO&INPIT
摘要翻译：要解决的问题：提供用于移植的骨/软骨样结构，其中不使用支架制备的软骨样结构和人造骨材料被牢固地结合，以及制造它的方法。解决方案：作为解决问题的敏锐检查的结果，完成了本发明。也就是说，在本发明中，通过将动物细胞悬浮液插入到与离心旋转的径向正交的平坦的内底面的容器中之后，在容器中培养通过进行离心沉降而获得的片状动物细胞聚集体，进行离心分离，开始培养后，将骨状固体置于培养容器的底面的细胞聚集体上，从而形成骨/软骨状结构体。版权所有（C）2011，JPO＆INPIT

8. 发明专利

JP2009022264A Medium for cell culture containing fish serum 审中-公开
标题翻译：用于含有鱼血清的细胞培养基
公开(公告)号：JP2009022264A
公开(公告)日：2009-02-05
申请号：JP2007216494
申请日：2007-07-23
申请人： Mutsumi Takagi , 睦高木
发明人： TAKAGI MUTSUMI , TSUKADA RYOHEI , FUJIWARA SEIJI
IPC分类号： C12N5/07 , C12N5/071
摘要： PROBLEM TO BE SOLVED: To provide a medium for cell culture, wherein the medium has no risk that humans are contaminated with pathogens on the basis of adding the serum of mammalian animals such as cattle to the medium, and can achieve excellent culture results. SOLUTION: Provided are a medium for animal cell culture containing fish serum instead of mammalian serum, a medium for animal cell culture containing fish serum at low concentration less than 5% V/V, a medium for animal cell culture containing mammalian serum at concentration less than 0.5%, and a medium for animal cell culture containing fish serum heat-treated at a temperature ranging from 40°C to 70°C. COPYRIGHT: (C)2009,JPO&INPIT
摘要翻译：要解决的问题：提供用于细胞培养的培养基，其中基于将哺乳动物如牛的血清添加到培养基中而不存在人被病原体污染的风险，并且可以实现优异的培养结果。提供了含有鱼血清而不是哺乳动物血清的动物细胞培养基，用于含有低于5％V / V的低浓度的鱼血清的动物细胞培养基的培养基，含有哺乳动物血清的动物细胞培养基浓度小于0.5％，以及含有在40℃至70℃的温度下热处理的鱼血清的动物细胞培养基。版权所有（C）2009，JPO＆INPIT

9. 发明专利

JP2012152206A Cartilage defect model 审中-公开
标题翻译：卡具缺陷模型
公开(公告)号：JP2012152206A
公开(公告)日：2012-08-16
申请号：JP2011029150
申请日：2011-01-25
申请人： Mutsumi Takagi , 睦高木
发明人： TAKAGI MUTSUMI , IWAI YOSHISUKE
IPC分类号： C12N5/077 , C12Q1/02
摘要： PROBLEM TO BE SOLVED: To provide a small piece of animal cartilage suitable for evaluating cytokine and MSC (mesenchymal stem cell), and an evaluation system for cartilage defect repair with higher throughput requiring less number of required cells using the same.SOLUTION: There are provided at least the small piece of animal cartilage prepared by hollowing out the cartilage from its surface layer to its lower bone, and the evaluation system for cartilage defect repair using the same.

10. 发明专利

JP2008054690A Automatic cell-culturing device and method for using the same 有权
标题翻译：自动细胞培养装置及其使用方法
公开(公告)号：JP2008054690A
公开(公告)日：2008-03-13
申请号：JP2007265219
申请日：2007-10-11
申请人： Kawasaki Heavy Ind Ltd , Mutsumi Takagi , 川崎重工業株式会社 , 睦高木
发明人： TAKAGI MUTSUMI , NAKAJIMA KATSUMI , DOI TOSHINAO , OGATA TAKAMASA
IPC分类号： C12M1/00 , C12M1/12 , C12N1/00
CPC分类号： C12M41/14 , C12M23/50 , C12M37/00 , C12M41/48
摘要： PROBLEM TO BE SOLVED: To provide an automatic cell-culturing device not causing cross-contamination even if being used for cell culture of a plurality of testees, and enabling the device including a refrigerating and preserving part and a normal-temperature preserving part to be completely sterilized.
SOLUTION: The automatic cell-culturing device is equipped with an operation part 11 for carrying out culture operation required for the cell culture of the testee, an incubator part 14 for culturing the cells, a preserving part for preserving reagents and incubating instruments required for the culture, and a steam-feeding part 22 for carrying out autoclave sterilization. The incubator part and the preserving part are each communicated with the operation part, and have each a sealing door 18a-c between the part and the operation part. The sterilization steam is fed from the steam-feeding part to the operation part and one of the incubator part and the preservation part communicated with the operation part by selectively opening and closing the sealing doors.
COPYRIGHT: (C)2008,JPO&INPIT
摘要翻译：要解决的问题：提供即使用于多个受试者的细胞培养物也不会引起交叉污染的自动细胞培养装置，并且能够实现包括冷藏和保存部分和常温保存的装置部分待完全消毒。解决方案：自动细胞培养装置配备有用于进行受试者的细胞培养所需的培养操作的操作部11，培养细胞的培养部14，保存试剂的保存部和培养器具用于进行高压灭菌的蒸汽供给部22。培养箱部分和保存部分各自与操作部分连通，并且在部件和操作部分之间具有密封门18a-c。灭菌蒸汽从蒸汽供给部供给到操作部，通过选择性地打开和关闭密封门，将培养器部和保存部中的一个与操作部连通。版权所有（C）2008，JPO＆INPIT

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式