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1. 发明申请

US20130244882A1 MULTIPLEX NUCLEIC ACID REACTIONS 有权
公开(公告)号：US20130244882A1
公开(公告)日：2013-09-19
申请号：US13604872
申请日：2012-09-06
申请人： Arnold Oliphant , John R. Stuelpnagel , Mark S. Chee , Scott L. Butler , Jian-Bing Fan , Min-Jui Richard Shen
发明人： Arnold Oliphant , John R. Stuelpnagel , Mark S. Chee , Scott L. Butler , Jian-Bing Fan , Min-Jui Richard Shen
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6876 , C12Q1/6834 , C12Q1/6837 , C12Q2600/16 , Y10T436/143333 , C12Q2525/155 , C12Q2563/131 , C12Q2563/179
摘要： A method for detecting nucleic acids by (a) providing a sample having target nucleic acids, each nucleic acid having contiguous first, second, and third domains; (b) contacting the sample with probe sets to form hybridization complexes, wherein each probe set includes (i) a first probe having a sequence that is complementary to the first domain; and (ii) a second probe having a sequence substantially complementary to the third domain; (c) extending the first probes along the second domains of the complexes while the complexes are immobilized on a solid support; (d) ligating the extended first probes to the second probes to form templates; (e) amplifying the templates with primers that are complementary to the first and second priming sequences to produce amplicons; and (f) detecting the amplicons on the surface of a nucleic acid array.

2. 发明授权

US08999642B2 Methods for selecting and amplifying polynucleotides 有权
标题翻译：选择和扩增多核苷酸的方法
公开(公告)号：US08999642B2
公开(公告)日：2015-04-07
申请号：US13387078
申请日：2009-08-25
申请人： Andrea Sabot , Roberto Rigatti , Min-Jui Richard Shen
发明人： Andrea Sabot , Roberto Rigatti , Min-Jui Richard Shen
IPC分类号： C12Q1/68 , C40B50/06 , C40B50/14 , C07H21/02 , C07H21/04
CPC分类号： C12N15/1068 , C12Q1/6806 , C12Q1/6837 , C12Q1/6874 , C12Q2565/543 , C12Q2565/507 , C12Q2531/107
摘要： The invention provides methods for controlling the density of different molecular species on the surface of a solid support. A first mixture of different molecular species is attached to a solid support under conditions to attach each species at a desired density, thereby producing a derivatized support having attached capture molecules. The derivatized support is treated with a second mixture of different molecular species, wherein different molecular species in the second mixture bind specifically to the different capture molecules attached to the solid support. One or more of the capture molecules can be reversibly modified such that the capture molecules have a different activity before and after the second mixture of molecular species are attached. In particular embodiments, the different molecular species are nucleic acids that are reversibly modified to have different activity in an amplification reaction.
摘要翻译：本发明提供了控制固体支持物表面上不同分子种类的密度的方法。将不同分子种类的第一混合物连接到固体支持物上，以便以期望的密度附着每种物质，从而产生具有附着捕获分子的衍生化载体。衍生的载体用不同分子种类的第二混合物处理，其中第二混合物中的不同分子物质特异性地结合到固相载体上的不同捕获分子。一个或多个捕获分子可以被可逆地修饰，使得捕获分子在分子物质的第二混合物附着之前和之后具有不同的活性。在具体实施方案中，不同的分子种类是可逆修饰以在扩增反应中具有不同活性的核酸。

3. 发明申请

US20100015626A1 MULTIPLEX NUCLEIC ACID REACTIONS 审中-公开
标题翻译：多重核酸反应
公开(公告)号：US20100015626A1
公开(公告)日：2010-01-21
申请号：US12507022
申请日：2009-07-21
申请人： Arnold Oliphant , John R. Stuelpnagel , Mark S. Chee , Scott L. Butler , Jian-Bing Fan , Min-Jui Richard Shen
发明人： Arnold Oliphant , John R. Stuelpnagel , Mark S. Chee , Scott L. Butler , Jian-Bing Fan , Min-Jui Richard Shen
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6876 , C12Q1/6834 , C12Q1/6837 , C12Q2600/16 , Y10T436/143333 , C12Q2525/155 , C12Q2563/131 , C12Q2563/179
摘要： The invention is directed to a variety of multiplexing methods used to amplify and/or genotype a variety of samples simultaneously.
摘要翻译：本发明涉及用于同时扩增和/或基因型多种样品的多种多样化方法。

4. 发明申请

US20080242555A1 MULTIPLEX NUCLEIC ACID REACTIONS 有权
标题翻译：多重核酸反应
公开(公告)号：US20080242555A1
公开(公告)日：2008-10-02
申请号：US12032581
申请日：2008-02-15
申请人： Min-Jui Richard Shen , Arnold Oliphant , Scott L. Butler , John R. Stuelpnagel , Mark S. Chee , Kenneth M. Kuhn , Jian-Bing Fan
发明人： Min-Jui Richard Shen , Arnold Oliphant , Scott L. Butler , John R. Stuelpnagel , Mark S. Chee , Kenneth M. Kuhn , Jian-Bing Fan
IPC分类号： C40B30/04
CPC分类号： C12Q1/6827 , C12Q1/6858 , C12Q2535/125 , C12Q2537/143 , C12Q2561/125 , C12Q2565/515
摘要： The invention is directed to a variety of multiplexing methods used to amplify and/or genotype a variety of samples simultaneously.
摘要翻译：本发明涉及用于同时扩增和/或基因型多种样品的多种多样化方法。

5. 发明申请

US20130071879A1 AMPLIFICATION METHODS TO MINIMISE SEQUENCE SPECIFIC BIAS 有权
标题翻译：最小化序列特异性偏差的放大方法
公开(公告)号：US20130071879A1
公开(公告)日：2013-03-21
申请号：US13580336
申请日：2011-02-23
申请人： Roberto Rigatti , Jonathan Mark Boutell , Min-Jui Richard Shen
发明人： Roberto Rigatti , Jonathan Mark Boutell , Min-Jui Richard Shen
IPC分类号： C12P19/34 , C09K3/00
CPC分类号： C12P19/34 , C09K3/00 , C12Q1/686 , C12Q2565/518 , C12Q2527/137
摘要： Methods for amplifying nucleic acids are provided. The methods can be used to minimise sequence specific bias caused by the preferential amplification of certain nucleic acid sequences. Methods are described which can lower the efficiency of AT rich templates relative to GC rich templates, thereby minimising GC bias during amplification reactions with multiple templates of different sequence. The methods are suited to solid phase amplification, for example, utilising flow cells.
摘要翻译：提供了扩增核酸的方法。这些方法可以用于最小化由某些核酸序列的优先扩增引起的序列特异性偏差。描述了可以降低AT富含模板相对于GC富集模板的效率的方法，由此使具有不同序列的多个模板的扩增反应期间的GC偏差最小化。该方法适用于固相扩增，例如利用流动池。

6. 发明申请

US20120196758A1 METHOD FOR NUCLEOTIDE DETECTION 有权
标题翻译：核子检测方法
公开(公告)号：US20120196758A1
公开(公告)日：2012-08-02
申请号：US13018255
申请日：2011-01-31
申请人： Kay KLAUSING , Vincent Smith , Min-Jui Richard Shen , John Moore , Kevin Hall
发明人： Kay KLAUSING , Vincent Smith , Min-Jui Richard Shen , John Moore , Kevin Hall
IPC分类号： C40B20/04 , C40B30/00 , C12Q1/68
CPC分类号： C12Q1/6874 , C12N15/1003 , C12Q1/6832 , C12Q1/6869 , C40B20/04 , C12Q2523/319 , C12Q2527/125 , C12Q2527/127
摘要： A method of inhibiting light-induced degradation of nucleic acids includes irradiating a portion of the nucleic acids in the presence of a detection solution comprising a polyphenolic compound. A method of detecting a nucleic acid having a fluorescent tag includes irradiating at least a portion of the nucleic acid with light of a suitable wavelength to induce a fluorescence emission and detecting the fluorescence emission. Optionally, the polyphenolic compound is gallic acid, a lower alkyl ester thereof, or mixtures thereof. A kit includes one or more nucleotides, an enzyme capable of catalyzing incorporation of the nucleotides into a nucleic acid strand and a polyphenolic compound suitable for preparing a detection solution.
摘要翻译：抑制光诱导的核酸降解的方法包括在存在包含多酚化合物的检测溶液的情况下照射一部分核酸。检测具有荧光标签的核酸的方法包括用合适波长的光照射核酸的至少一部分以诱导荧光发射并检测荧光发射。任选地，多酚化合物是没食子酸，其低级烷基酯或其混合物。试剂盒包括一个或多个核苷酸，能够催化将核苷酸掺入核酸链的酶和适于制备检测溶液的多酚化合物。

7. 发明申请

US20100311064A1 MULTIPLEX NUCLEIC ACID REACTIONS 有权
公开(公告)号：US20100311064A1
公开(公告)日：2010-12-09
申请号：US12790757
申请日：2010-05-28
申请人： Arnold Oliphant , John R. Stuelpnagel , Mark S. Chee , Scott L. Butler , Jian-Bing Fan , Min-Jui Richard Shen
发明人： Arnold Oliphant , John R. Stuelpnagel , Mark S. Chee , Scott L. Butler , Jian-Bing Fan , Min-Jui Richard Shen
IPC分类号： C12Q1/68 , C12P19/34 , G01N33/00
CPC分类号： C12Q1/6876 , C12Q1/6834 , C12Q1/6837 , C12Q2600/16 , Y10T436/143333 , C12Q2525/155 , C12Q2563/131 , C12Q2563/179
摘要： The invention is directed to a variety of multiplexing methods used to amplify and/or genotype a variety of samples simultaneously.

8. 发明申请

US20100151473A1 METHODS AND COMPOSITIONS FOR HYBRIDIZING NUCLEIC ACIDS 审中-公开
标题翻译：用于杂交核酸的方法和组合物
公开(公告)号：US20100151473A1
公开(公告)日：2010-06-17
申请号：US12634531
申请日：2009-12-09
申请人： Joanne M. Yeakley , Ross Edward Lenta , Min-Jui Richard Shen
发明人： Joanne M. Yeakley , Ross Edward Lenta , Min-Jui Richard Shen
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6832 , C12Q2527/125
摘要： Methods and compositions for hybridizing nucleic acids are disclosed herein. Also disclosed herein are methods and compositions to detect a nucleic acid provided to an array.
摘要翻译：本文公开了用于杂交核酸的方法和组合物。本文还公开了检测提供给阵列的核酸的方法和组合物。

9. 发明授权

US07582420B2 Multiplex nucleic acid reactions 有权
标题翻译：多重核酸反应
公开(公告)号：US07582420B2
公开(公告)日：2009-09-01
申请号：US10194958
申请日：2002-07-12
申请人： Arnold Oliphant , John R. Stuelpnagel , Mark S. Chee , Scott L. Butler , Jian-Bing Fan , Min-Jui Richard Shen
发明人： Arnold Oliphant , John R. Stuelpnagel , Mark S. Chee , Scott L. Butler , Jian-Bing Fan , Min-Jui Richard Shen
IPC分类号： C12Q1/68 , C12P19/34 , C07H21/04
CPC分类号： C12Q1/6876 , C12Q1/6834 , C12Q1/6837 , C12Q2600/16 , Y10T436/143333 , C12Q2525/155 , C12Q2563/131 , C12Q2563/179
摘要： The invention is directed to a variety of multiplexing methods used to amplify and/or genotype a variety of samples simultaneously.
摘要翻译：本发明涉及用于同时扩增和/或基因型多种样品的多种多样化方法。

10. 发明授权

US08906626B2 Multiplex nucleic acid reactions 有权
标题翻译：多重核酸反应
公开(公告)号：US08906626B2
公开(公告)日：2014-12-09
申请号：US13604872
申请日：2012-09-06
申请人： Arnold Oliphant , John R. Stuelpnagel , Mark S. Chee , Scott L. Butler , Jian-Bing Fan , Min-Jui Richard Shen
发明人： Arnold Oliphant , John R. Stuelpnagel , Mark S. Chee , Scott L. Butler , Jian-Bing Fan , Min-Jui Richard Shen
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6876 , C12Q1/6834 , C12Q1/6837 , C12Q2600/16 , Y10T436/143333 , C12Q2525/155 , C12Q2563/131 , C12Q2563/179
摘要： A method for detecting nucleic acids by (a) providing a sample having target nucleic acids, each nucleic acid having contiguous first, second, and third domains; (b) contacting the sample with probe sets to form hybridization complexes, wherein each probe set includes (i) a first probe having a sequence that is complementary to the first domain; and (ii) a second probe having a sequence substantially complementary to the third domain; (c) extending the first probes along the second domains of the complexes while the complexes are immobilized on a solid support; (d) ligating the extended first probes to the second probes to form templates; (e) amplifying the templates with primers that are complementary to the first and second priming sequences to produce amplicons; and (f) detecting the amplicons on the surface of a nucleic acid array.
摘要翻译：一种用于通过（a）提供具有靶核酸的样品来检测核酸的方法，每个核酸具有连续的第一，第二和第三结构域; （b）使样品与探针组接触以形成杂交复合物，其中每个探针组包括（i）具有与第一结构域互补的序列的第一探针; 和（ii）具有与第三结构域基本上互补的序列的第二探针; （c）沿络合物的第二结构域扩展第一探针，同时将复合物固定在固体支持物上; （d）将扩展的第一探针连接到第二探针以形成模板; （e）用与第一和第二引物序列互补的引物扩增模板以产生扩增子; 和（f）检测核酸阵列表面上的扩增子。

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