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1. 发明申请

US20060257997A1 High throughput synthesis system and synthesizer for automatically performing the system 审中-公开
标题翻译：高吞吐量合成系统和合成器，用于自动执行系统
公开(公告)号：US20060257997A1
公开(公告)日：2006-11-16
申请号：US10554434
申请日：2004-04-23
申请人： Yaeta Endo , Tatsuya Sawasaki , Tomio Ogasawara , Riyo Morishita , Mihoro Saeki , Tomohisa Sato , Aya Kitamoto
发明人： Yaeta Endo , Tatsuya Sawasaki , Tomio Ogasawara , Riyo Morishita , Mihoro Saeki , Tomohisa Sato , Aya Kitamoto
IPC分类号： C12M3/00
CPC分类号： C12P21/02 , C12P19/34
摘要： To develop system technology for a high throughput in vitro synthesis reaction method for biopolymers such as proteins, RNA, and the like. In detail, a cell-free synthesis system wherein a template substance is used as a raw material, and an automatic synthesizer using the system, includes the following systems, control systems therefor, or combinations thereof; 1) to make a template substance, a substrate, and a reaction solution into contact so as to lead to a synthetic reaction, 2) to put the reaction system outside of the synthetic reaction system to perform dilution treatment of the solution approximately around the decrease in the synthesis velocity, approximately around the stop of the synthetic reaction, or in the middle thereof, 3) to perform concentration treatment subsequent to the dilution treatment, and 4) to put the reaction system back into the synthetic reaction system, or 1) to make a template substance, a substrate, and a reaction solution into contact so as to lead to a synthetic reaction, 2) to put the reaction system outside of the synthetic reaction system to perform concentration treatment of the solution approximately around the decrease in the synthesis velocity, approximately around the stop of the synthetic reaction, or in the middle thereof, 3) to perform dilution treatment subsequent to the concentration treatment, and 4) to put the diluted reaction system back into the synthetic reaction system.
摘要翻译：开发用于生物聚合物如蛋白质，RNA等的高通量体外合成反应方法的系统技术。具体而言，其中使用模板物质作为原料的无细胞合成系统和使用该系统的自动合成器包括以下系统，其控制系统或其组合; 1）使模板物质，底物和反应溶液接触以导致合成反应，2）将反应体系置于合成反应体系外部，以进行大约减少的溶液的稀释处理在合成速度约合成反应停止或其中间，3）进行稀释处理后的浓缩处理，4）使反应体系回到合成反应体系中，或1）使模板物质，底物和反应溶液接触以导致合成反应，2）将反应体系置于合成反应体系外部，以在约合成速度大约在合成反应停止时，或在其中间，3）进行浓缩处理后的稀释处理，和4）将稀释的反应体系放回到合成反应体系中。

2. 发明申请

US20050255542A1 Method for producing by cell-free protein synthesis system using thioredoxin-fused protein expression vector 失效
标题翻译：使用硫氧还蛋白融合蛋白表达载体的无细胞蛋白质合成系统生产方法
公开(公告)号：US20050255542A1
公开(公告)日：2005-11-17
申请号：US10992360
申请日：2004-11-19
申请人： Mikako Shirouzu , Goushi Ishihara , Mihoro Saeki , Mie Goto , Kaori Tajima , Takanori Kigawa , Shigeyuki Yokoyama
发明人： Mikako Shirouzu , Goushi Ishihara , Mihoro Saeki , Mie Goto , Kaori Tajima , Takanori Kigawa , Shigeyuki Yokoyama
IPC分类号： C07K14/705 , C12N9/02 , C12N15/62 , C12P21/00 , C12P21/02 , C12P21/06 , C12P21/04
CPC分类号： C12N15/62 , C07K14/705 , C07K2319/00 , C07K2319/033 , C07K2319/21 , C07K2319/23 , C07K2319/35 , C07K2319/50 , C12N9/0036 , C12P21/00 , C12Y108/01008
摘要： A fusion protein of thioredoxin with a target protein is expressed in a cell-free protein synthesis system to thereby synthesize at least a part of the fusion protein as a soluble protein. It is preferable that the target protein is a highly hydrophobic protein such as a membrane protein. In particular, a membrane protein such a G protein-coupled receptor can be produced in a state being highly soluble and capable of forming a biologically active three dimensional structure. Also, provided a recombinant vector for producing such a membrane protein in a cell-free protein synthesis system.
摘要翻译：将硫氧还蛋白与靶蛋白的融合蛋白在无细胞蛋白质合成系统中表达，从而合成至少一部分融合蛋白作为可溶性蛋白质。目标蛋白质优选为高度疏水性的蛋白质，例如膜蛋白质。特别地，可以在高度可溶性且能够形成生物活性三维结构的状态下产生诸如G蛋白偶联受体的膜蛋白。此外，提供了在无细胞蛋白质合成系统中产生这种膜蛋白质的重组载体。

3. 发明授权

US07253144B2 Method for producing by cell-free protein synthesis system using thioredoxin-fused protein expression vector 失效
标题翻译：使用硫氧还蛋白融合蛋白表达载体的无细胞蛋白质合成系统生产方法
公开(公告)号：US07253144B2
公开(公告)日：2007-08-07
申请号：US10992360
申请日：2004-11-19
申请人： Mikako Shirouzu , Goushi Ishihara , Mihoro Saeki , Mie Goto , Kaori Tajima , Takanori Kigawa , Shigeyuki Yokoyama
发明人： Mikako Shirouzu , Goushi Ishihara , Mihoro Saeki , Mie Goto , Kaori Tajima , Takanori Kigawa , Shigeyuki Yokoyama
IPC分类号： C12N15/70 , C12N15/63 , C07K14/705 , C07K21/04 , A61K38/00
CPC分类号： C12N15/62 , C07K14/705 , C07K2319/00 , C07K2319/033 , C07K2319/21 , C07K2319/23 , C07K2319/35 , C07K2319/50 , C12N9/0036 , C12P21/00 , C12Y108/01008
摘要： We claim a method of expression a thioredoxin-fused membrane protein comprising expressing said thioredoxin-fused membrane protein in a cell-free protein synthesis system in the presence of a non-ionic detergent. It is preferable that the fused membrane protein which is a highly hydrophobic protein, e.g., a G protein-coupled receptor, can be produced in a state being highly soluble and capable of forming a biologically active three-dimensional structure. Also, this invention provides a recombinant polynucleotide encoding said thioredoxin-fused membrane protein which is expressed in the cell-free protein synthesis system.
摘要翻译：我们要求一种表达硫氧还蛋白融合膜蛋白的方法，包括在非离子型洗涤剂存在下，在无细胞蛋白质合成系统中表达所述硫氧还蛋白融合的膜蛋白。作为高度疏水性的蛋白质，例如G蛋白偶联受体的融合膜蛋白质，优选可以高度溶解，能够形成生物活性的三维结构体。此外，本发明提供编码在无细胞蛋白质合成系统中表达的硫氧还蛋白融合膜蛋白的重组多核苷酸。

4. 发明授权

US06180602B2 Human novel cDNA, TGF-beta superfamily protein encoded thereby and the use of immunosuppressive agent 失效
标题翻译：人类新型cDNA，由此编码的TGF-β超家族蛋白和使用免疫抑制剂
公开(公告)号：US06180602B2
公开(公告)日：2001-01-30
申请号：US08775882
申请日：1997-01-02
申请人： Seishi Kato , Suwan Oh , Shingo Sekine , Mihoro Saeki , Midori Kobayashi , Mika Yada , Tomoko Tsuji , Hitoshi Ohmori
发明人： Seishi Kato , Suwan Oh , Shingo Sekine , Mihoro Saeki , Midori Kobayashi , Mika Yada , Tomoko Tsuji , Hitoshi Ohmori
IPC分类号： A61K3818
CPC分类号： C12N9/1007 , C07K14/47 , C12N9/14 , Y10S530/868
摘要： Isolated cDNAs derived from mRNAs expressed in human cells are provided, as are DNAs and RNAs comprising their nucleotide sequences, and vectors for expressing the cDNAs. The cDNAs encode proteins which have functions similar to known proteins.
摘要翻译：提供了源自人细胞中表达的mRNA的分离的cDNA，以及包含其核苷酸序列的DNA和RNA以及用于表达cDNA的载体。 cDNA编码具有与已知蛋白质相似功能的蛋白质。

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