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    • 2. 发明专利
      DE2012189C3  未知
    • DE2012189C3
    • 1974-10-10
    • DE2012189
    • 1970-03-14
    • MCLAUGHLIN GORMLEY KING COMPANY, MINNEAPOLIS, MINN. (V.ST.A.)
    • WELLS, FRANK E., KANSAS CITY, MO. (V.ST.A.)
    • A01N63/00 , C12N1/00 , C12N1/20 , C12N7/00 , C12K7/00 , A01N15/00
    • 1300447 Replicating insect viruses McLAUGHLIN GORMLEY KING CO 16 March 1970 [17 March 1969] 12573/70 Heading C6F [Also in Division A5] In the production of an insecticidal product, an insect infective virus is inoculated into a culture comprising cells or cell material of a host micro-organism, such cells or cell material being capable of further metabolism and being chosen to support replication of the virus, and the virus is replicated in the culture. Any species of yeasts, bacteria, mycobacteria, fungi, algae, protozoa or like which may be readily grown in the laboratory in a synthetic or semi-synthetic medium under reasonable conditions can be considered for replication trials. Typically, the virus is one of the infective viruses for insects of the order Lepidoptera (specified) and the micro-organism is a fungus of the family Saccharomycetaceae or a bacterium of the orders Actimomycetales, Eubacteriales and Pseudomenadales. The micro-organism is first activated in or on a nutrient medium until about completion of its logarithmic growth phase, thereafter the cell walls of the micro-organism are at least partially disrupted (while still maintaining a viable culture of host cells) by specified chemical, mechanical, physical or enzymatic means, but preferably by use of a so-called transformation medium of high osmotic pressure, to permit or to improve infection by the virus of the cytoplasm and /or cytoplasmic membrane of the cells of the micro-organism, and replication of the virus (after inoculation) may be effected in the transformation medium. This consists of 5-20% by weight of sucrose and small amounts of magnesium sulphate, sodium acetate and tryptone. The resulting product may be concentrated or dried for use as a viral insecticide. In commercial use the product may be diluted with a suitable carrier or diluent such as water or other liquid, bentonite, clay or talc, and may be mixed with conventional sticking agents such as carboxymethyl cellulose, stick liquor, gums or acrylic resins. These additives may be incorporated with the virus host-cell mixture prior to concentration and drying. An example is directed to the replication of fall armyworm polyhedrosis virus on a strain of Saccharamyces cerevisiae isolated from the gut of a corn earworm larva. The product was found to be capable of establishing viral infections in fall armyworm, southern armyworm and corn earworm insects found in crops. Other examples relate to the cultivation of viruses from these insects upon Bacillus thuringiensis and Bacillus subtilis.
    • 基本信息 添加关注 加入工作空间 PDF全文 PDF下载 全文下载 相似专利 双屏查看 权利要求书 说明书全文 Espacenet 法律信息 同族专利 专利引用
    • 3. 发明专利
      DE2012189B2  未知
    • DE2012189B2
    • 1974-03-07
    • DE2012189
    • 1970-03-14
    • MCLAUGHLIN GORMLEY KING COMPANY, MINNEAPOLIS, MINN. (V.ST.A.)
    • WELLS, FRANK E., KANSAS CITY, MO. (V.ST.A.)
    • A01N63/00 , C12N1/00 , C12N1/20 , C12N7/00 , C12K7/00
    • 1300447 Replicating insect viruses McLAUGHLIN GORMLEY KING CO 16 March 1970 [17 March 1969] 12573/70 Heading C6F [Also in Division A5] In the production of an insecticidal product, an insect infective virus is inoculated into a culture comprising cells or cell material of a host micro-organism, such cells or cell material being capable of further metabolism and being chosen to support replication of the virus, and the virus is replicated in the culture. Any species of yeasts, bacteria, mycobacteria, fungi, algae, protozoa or like which may be readily grown in the laboratory in a synthetic or semi-synthetic medium under reasonable conditions can be considered for replication trials. Typically, the virus is one of the infective viruses for insects of the order Lepidoptera (specified) and the micro-organism is a fungus of the family Saccharomycetaceae or a bacterium of the orders Actimomycetales, Eubacteriales and Pseudomenadales. The micro-organism is first activated in or on a nutrient medium until about completion of its logarithmic growth phase, thereafter the cell walls of the micro-organism are at least partially disrupted (while still maintaining a viable culture of host cells) by specified chemical, mechanical, physical or enzymatic means, but preferably by use of a so-called transformation medium of high osmotic pressure, to permit or to improve infection by the virus of the cytoplasm and /or cytoplasmic membrane of the cells of the micro-organism, and replication of the virus (after inoculation) may be effected in the transformation medium. This consists of 5-20% by weight of sucrose and small amounts of magnesium sulphate, sodium acetate and tryptone. The resulting product may be concentrated or dried for use as a viral insecticide. In commercial use the product may be diluted with a suitable carrier or diluent such as water or other liquid, bentonite, clay or talc, and may be mixed with conventional sticking agents such as carboxymethyl cellulose, stick liquor, gums or acrylic resins. These additives may be incorporated with the virus host-cell mixture prior to concentration and drying. An example is directed to the replication of fall armyworm polyhedrosis virus on a strain of Saccharamyces cerevisiae isolated from the gut of a corn earworm larva. The product was found to be capable of establishing viral infections in fall armyworm, southern armyworm and corn earworm insects found in crops. Other examples relate to the cultivation of viruses from these insects upon Bacillus thuringiensis and Bacillus subtilis.
    • 基本信息 添加关注 加入工作空间 PDF全文 PDF下载 全文下载 相似专利 双屏查看 权利要求书 说明书全文 Espacenet 法律信息 同族专利 专利引用
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