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1. 发明授权

US08084212B2 Assay for Mycobacterium avium/intracellulare nucleic acid 有权
标题翻译：分析鸟分枝杆菌/胞内分枝杆菌核酸
公开(公告)号：US08084212B2
公开(公告)日：2011-12-27
申请号：US12887403
申请日：2010-09-21
申请人： Edgar Ong , Maurice Exner
发明人： Edgar Ong , Maurice Exner
IPC分类号： C12Q1/68 , C12P19/34 , C07H19/34
CPC分类号： C12Q1/689 , C12Q2600/16
摘要： Disclosed is a method for determining the presence of Mycobacterium avium complex nucleic acids in a biological sample. In particular, the IS1245 gene of M. avium and the DT1 gene of M. intracellulare are detected, preferably following amplification. In addition, the method distinguishes between species of M. avium and M. intracellulare. Also described are oligonucleotides that can be used as primers to amplify target genes such as IS1245 and DT1 genes and as probes as well as kits containing the oligonucleotides.
摘要翻译：公开了一种用于确定生物样品中鸟分枝杆菌复合物核酸的存在的方法。特别地，优选在扩增后检测鸟分枝杆菌的IS1245基因和胞内分枝杆菌的DT1基因。此外，该方法区分了鸟分枝杆菌和胞内分枝杆菌的种类。还描述了可用作引物扩增靶基因如IS1245和DT1基因以及作为探针的寡核苷酸以及含有寡核苷酸的试剂盒。

2. 发明申请

US20070141559A1 Methods for detecting and typing herpes simplex virus 审中-公开
标题翻译：检测和分类单纯疱疹病毒的方法
公开(公告)号：US20070141559A1
公开(公告)日：2007-06-21
申请号：US11314202
申请日：2005-12-20
申请人： Maurice Exner
发明人： Maurice Exner
IPC分类号： C12Q1/70 , C12Q1/68
CPC分类号： C12Q1/705
摘要： Detailed herein are methods of detecting herpes simplex virus and differentiating between types 1 and 2 by simultaneous detection of type-specific gene sequences. In particular aspects, individuals infected with HSV-1 can be distinguished from those infected with HSV-2 by amplification and detection of the HSV-1 glycoprotein B gene or the HSV-2 UL-8 gene. Primers and probes for the differential detection of HSV-1 and HSV-2 are provided.
摘要翻译：本文详细描述了通过同时检测类型特异性基因序列来检测单纯疱疹病毒并区分类型1和2之间的方法。在特定方面，通过HSV-1糖蛋白B基因或HSV-2 UL-8基因的扩增和检测可以将感染HSV-1的个体与感染HSV-2的个体区分开。提供用于HSV-1和HSV-2的差异检测的引物和探针。

3. 发明授权

US09168523B2 Systems and methods for detecting the presence of a selected volume of material in a sample processing device 有权
标题翻译：用于检测样品处理装置中选定体积的材料的存在的系统和方法
公开(公告)号：US09168523B2
公开(公告)日：2015-10-27
申请号：US13474903
申请日：2012-05-18
申请人： Peter D. Ludowise , David A. Whitman , Kyle C. Armantrout , Maurice Exner , Lucien A. E. Jacky , Michelle Tabb
发明人： Peter D. Ludowise , David A. Whitman , Kyle C. Armantrout , Maurice Exner , Lucien A. E. Jacky , Michelle Tabb
IPC分类号： G01N21/00 , B01L3/00
CPC分类号： C12Q1/686 , B01L3/5027 , B01L3/502715 , B01L2200/0605 , B01L2300/087 , B01L2400/0409 , G01N21/6486 , G01N35/025 , G01N35/04 , G01N2035/0441
摘要： Systems and methods for processing sample processing devices. The system can include a sample processing device comprising a detection chamber, a motor configured to rotate the sample processing device about an axis of rotation, and an optical module operatively positioned relative to the sample processing device and configured to determine whether a selected volume of material is present in the detection chamber of the sample processing device. The method can include rotating the sample processing device about an axis of rotation, and determining whether a selected volume of material is present in the detection chamber, while rotating the sample processing device. In some embodiments, determining whether a selected volume of material is present can be performed by optically interrogating the detection chamber for an optical property of the material.
摘要翻译：用于处理样品处理装置的系统和方法。该系统可以包括样品处理装置，该样品处理装置包括检测室，被配置为围绕旋转轴线旋转样品处理装置的马达和相对于样品处理装置可操作地定位的光学模块，并配置成确定所选择的材料体积存在于样品处理装置的检测室中。该方法可以包括围绕旋转轴旋转样品处理装置，并且在旋转样品处理装置的同时确定所选择的材料体积是否存在于检测室中。在一些实施例中，确定所选择的材料体积是否存在可以通过光学询问该检测室以获得该材料的光学特性。

4. 发明申请

US20120293796A1 SYSTEMS AND METHODS FOR DETECTING THE PRESENCE OF A SELECTED VOLUME OF MATERIAL IN A SAMPLE PROCESSING DEVICE 有权
标题翻译：用于检测样品处理装置中选择的材料体积的存在的系统和方法
公开(公告)号：US20120293796A1
公开(公告)日：2012-11-22
申请号：US13474903
申请日：2012-05-18
申请人： Peter D. Ludowise , David A. Whitman , Kyle C. Armantrout , Maurice Exner , Lucien A.E. Jacky , Michelle Tabb
发明人： Peter D. Ludowise , David A. Whitman , Kyle C. Armantrout , Maurice Exner , Lucien A.E. Jacky , Michelle Tabb
IPC分类号： G01N21/01
CPC分类号： C12Q1/686 , B01L3/5027 , B01L3/502715 , B01L2200/0605 , B01L2300/087 , B01L2400/0409 , G01N21/6486 , G01N35/025 , G01N35/04 , G01N2035/0441
摘要： Systems and methods for processing sample processing devices. The system can include a sample processing device comprising a detection chamber, a motor configured to rotate the sample processing device about an axis of rotation, and an optical module operatively positioned relative to the sample processing device and configured to determine whether a selected volume of material is present in the detection chamber of the sample processing device. The method can include rotating the sample processing device about an axis of rotation, and determining whether a selected volume of material is present in the detection chamber, while rotating the sample processing device. In some embodiments, determining whether a selected volume of material is present can be performed by optically interrogating the detection chamber for an optical property of the material.
摘要翻译：用于处理样品处理装置的系统和方法。该系统可以包括样品处理装置，该样品处理装置包括检测室，被配置为围绕旋转轴线旋转样品处理装置的马达和相对于样品处理装置可操作地定位的光学模块，并配置成确定所选择的材料体积存在于样品处理装置的检测室中。该方法可以包括围绕旋转轴旋转样品处理装置，并且在旋转样品处理装置的同时确定所选择的材料体积是否存在于检测室中。在一些实施例中，确定所选择的材料体积是否存在可以通过光学询问该检测室以获得该材料的光学特性。

5. 发明申请

US20110027792A1 ASSAY FOR MYCOBACTERIUM AVIUM/INTRACELLULARE NUCLEIC ACID 有权
标题翻译：测定MYCOBACTERIUM AVIUM /细胞内核酸
公开(公告)号：US20110027792A1
公开(公告)日：2011-02-03
申请号：US12887403
申请日：2010-09-21
申请人： Edgar Ong , Maurice Exner
发明人： Edgar Ong , Maurice Exner
IPC分类号： C12Q1/68
CPC分类号： C12Q1/689 , C12Q2600/16
摘要： Disclosed is a method for determining the presence of Mycobacterium avium complex nucleic acids in a biological sample. In particular, the mig gene of M. avium and the DT1 gene of M. intracellulare are detected, preferably following amplification. In addition, the method distinguishes between species of M. avium and M. intracellulare. Also described are oligonucleotides that can be used as primers to amplify target genes such as mig and DT1 genes and as probes as well as kits containing the oligonucleotides.
摘要翻译：公开了一种用于确定生物样品中鸟分枝杆菌复合物核酸的存在的方法。特别地，优选在扩增后检测鸟分枝杆菌的mig基因和胞内分枝杆菌的DT1基因。此外，该方法区分了鸟分枝杆菌和胞内分枝杆菌的种类。还描述了可用作扩增靶基因如mig和DT1基因以及作为探针以及含有寡核苷酸的试剂盒的寡核苷酸。

6. 发明授权

US07824858B2 Assay for mycobacterium avium/intracellulare nucleic acid 有权
标题翻译：分析鸟分枝杆菌/胞内分枝杆菌核酸
公开(公告)号：US07824858B2
公开(公告)日：2010-11-02
申请号：US11338431
申请日：2006-01-23
申请人： Edgar Ong , Maurice Exner
发明人： Edgar Ong , Maurice Exner
IPC分类号： C12Q1/68 , C12P19/34 , C07H21/04
CPC分类号： C12Q1/689 , C12Q2600/16
摘要： Disclosed is a method for determining the presence of Mycobacterium avium complex nucleic acids in a biological sample. In particular, the mig gene of M. avium and the DT1 gene of M. intracellulare are detected, preferably following amplification. In addition, the method distinguishes between species of M. avium and M. intracellulare. Also described are oligonucleotides that can be used as primers to amplify target genes such as mig and DT1 genes and as probes as well as kits containing the oligonucleotides.
摘要翻译：公开了一种用于确定生物样品中鸟分枝杆菌复合物核酸的存在的方法。特别地，优选在扩增后检测鸟分枝杆菌的mig基因和胞内分枝杆菌的DT1基因。此外，该方法区分了鸟分枝杆菌和胞内分枝杆菌的种类。还描述了可用作扩增靶基因如mig和DT1基因以及作为探针以及含有寡核苷酸的试剂盒的寡核苷酸。

7. 发明授权

US07785786B2 Methods for detecting nucleic acids using multiple signals 有权
标题翻译：使用多种信号检测核酸的方法
公开(公告)号：US07785786B2
公开(公告)日：2010-08-31
申请号：US11337805
申请日：2006-01-23
申请人： Maurice Exner , Amy Rogers
发明人： Maurice Exner , Amy Rogers
IPC分类号： C12Q1/68 , C12P19/34 , C07H21/04
CPC分类号： C12Q1/6818 , C12Q2563/173 , C12Q2527/107
摘要： Disclosed is are methods for identifying a nucleic acid in a sample. In one example, the method includes: (a) contacting the nucleic acid in the sample with an oligonucleotide that is specific for the nucleic acid in the sample and that is labeled with at least a first fluorescent dye; (b) contacting the nucleic acid in the sample with a second fluorescent dye that is different from the first fluorescent dye, such that the second fluorescent dye interacts with the nucleic acid; (c) amplifying the nucleic acid if present in the sample; and (d) detecting the nucleic acid if present in the sample by observing fluorescence from the first fluorescent dye after the oligonucleotide hybridizes to the amplified nucleic acid and determining the melting temperature of the amplified nucleic acid by measuring the fluorescence of the second fluorescent dye. The second fluorescent dye may include a fluorescent intercalating agent.
摘要翻译：公开了用于鉴定样品中核酸的方法。在一个实例中，该方法包括：（a）使样品中的核酸与样品中核酸特异的寡核苷酸接触，并用至少第一荧光染料标记; （b）使样品中的核酸与不同于第一荧光染料的第二荧光染料接触，使得第二荧光染料与核酸相互作用; （c）如果存在于样品中则扩增核酸; 以及（d）通过在寡核苷酸与扩增的核酸杂交后通过观察来自第一荧光染料的荧光检测样品中的核酸，并通过测量第二荧光染料的荧光来测定扩增的核酸的解链温度。第二荧光染料可以包括荧光嵌入剂。

8. 发明授权

US06686162B2 Oligonucleotides and methods for detecting Borrelia burgdorferi 有权
标题翻译：寡核苷酸和检测勃氏疏螺旋体的方法
公开(公告)号：US06686162B2
公开(公告)日：2004-02-03
申请号：US10011340
申请日：2001-12-04
申请人： Maurice Exner , Hasnah Hamdan , Michael Lewinski
发明人： Maurice Exner , Hasnah Hamdan , Michael Lewinski
IPC分类号： C12Q168
CPC分类号： C12Q1/689 , C12Q2600/166 , Y02A50/57
摘要： The present invention provides methods and compositions for determining the presence and/or amount of Borrelia burgdorferi nucleic acids in a test sample related to Lyme disease. In particular, substantially purified oligonucleotide primers and probes are described that can be used for qualitatively and quantitatively detecting Borrelia burgdorferi nucleic acid in a test sample by amplification methods. The present invention also provides primers and probes for generating and detecting control nucleic acid sequences that provide a convenient method for assessing internal quality control of the Borrelia burgdorferi assay.
摘要翻译：本发明提供了用于确定与莱姆病相关的测试样品中伯氏疏螺旋体核酸的存在和/或量的方法和组合物。特别地，描述了基本上纯化的寡核苷酸引物和探针，其可以用于通过扩增方法在测试样品中定性和定量检测勃氏疏螺旋体核酸。本发明还提供用于产生和检测控制核酸序列的引物和探针，其提供用于评估疏螺旋体凝血酶测定的内部质量控制的方便方法。

9. 发明授权

US09464331B2 Direct amplification and detection of viral and bacterial pathogens 有权
标题翻译：直接扩增和检测病毒和细菌病原体
公开(公告)号：US09464331B2
公开(公告)日：2016-10-11
申请号：US14130705
申请日：2012-07-06
申请人： Maurice Exner , Luca Jacky , Yin-Peng Chen , Huong Mai , Michelle M. Tabb , Michael Aye
发明人： Maurice Exner , Luca Jacky , Yin-Peng Chen , Huong Mai , Michelle M. Tabb , Michael Aye
IPC分类号： C12Q1/68 , C12Q1/70 , C07H21/02
CPC分类号： C12Q1/701 , C12Q1/68 , C12Q1/6846 , C12Q1/686 , C12Q1/689 , C12Q1/70 , C12Q2527/101 , C12Q2527/125
摘要： Provided are methods for identifying the presence or absence of a target nucleic acid from a microorganism using direct amplification without a step of extraction of the nucleic acids, but retaining substantially the same specificity and sensitivity of methods assaying extracted nucleic acids. Further provided are reagent mixtures that allow for direct amplification of a sample, without the step of nucleic acid extraction.
摘要翻译：提供的是使用直接扩增从微生物鉴定靶核酸的存在或不存在的方法，而不提取核酸的步骤，但是保留测定提取的核酸的方法的基本相同的特异性和灵敏度。还提供允许直接扩增样品的试剂混合物，而不需要提取核酸步骤。

10. 发明授权

US09194006B2 Direct amplification and detection of viral and bacterial pathogens 有权
标题翻译：直接扩增和检测病毒和细菌病原体
公开(公告)号：US09194006B2
公开(公告)日：2015-11-24
申请号：US13543336
申请日：2012-07-06
申请人： Maurice Exner , Luca Jacky , Yin-Peng Chen , Huong Mai , Jules Chen , Michelle M. Tabb , Michael Aye , Emberlee Eleazar
发明人： Maurice Exner , Luca Jacky , Yin-Peng Chen , Huong Mai , Jules Chen , Michelle M. Tabb , Michael Aye , Emberlee Eleazar
IPC分类号： C12Q1/70 , C12Q1/68
CPC分类号： C12Q1/701 , C12Q1/68 , C12Q1/6846 , C12Q1/686 , C12Q1/689 , C12Q1/70 , C12Q2527/101 , C12Q2527/125
摘要： Provided herein are methods for identifying the presence or absence of a target nucleic acid from a microorganism using direct amplification without a step of extraction of the nucleic acids, but retaining substantially the same specificity and sensitivity of methods assaying extracted nucleic acids.
摘要翻译：本文提供的方法是使用直接扩增从微生物鉴定靶核酸的存在或不存在，而没有提取核酸的步骤，但是保留测定提取的核酸的方法的基本上相同的特异性和灵敏度。

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