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1. 发明授权

US06613581B1 Microfluidic analytic detection assays, devices, and integrated systems 有权
标题翻译：微流控分析检测，装置和集成系统
公开(公告)号：US06613581B1
公开(公告)日：2003-09-02
申请号：US09641468
申请日：2000-08-17
申请人： H. Garrett Wada , Matthew B. Murphy
发明人： H. Garrett Wada , Matthew B. Murphy
IPC分类号： G01N33543
CPC分类号： G01N33/543 , Y10T436/11
摘要： Methods of detecting a component of interest, such as a protein, in a microfluidic system are provided. The methods include the use of a component-binding moiety specific to the component of interest, such as an antibody, to detect the component of interest. Also included are microfluidic devices and integrated systems for performing such assays, including devices utilizing flowable or fixed particle sets.
摘要翻译：提供了在微流体系统中检测感兴趣的组分，例如蛋白质的方法。所述方法包括使用特异性的组分结合部分（例如抗体）来检测感兴趣的组分。还包括用于进行这种测定的微流体装置和集成系统，包括利用可流动或固定的颗粒组的装置。

2. 发明授权

US07723123B1 Western blot by incorporating an affinity purification zone 有权
标题翻译：通过掺入亲和纯化区的蛋白质印迹
公开(公告)号：US07723123B1
公开(公告)日：2010-05-25
申请号：US10159605
申请日：2002-05-31
申请人： Matthew B. Murphy , Robert S. Dubrow
发明人： Matthew B. Murphy , Robert S. Dubrow
IPC分类号： G01N33/558
CPC分类号： G01N33/54366
摘要： An upstream affinity purification region is used to bind one or more component of interest in a mixture of components prior to separating the mixture of components. Detection of the separated components and a released component of interest provide identification of the component of interest. In addition, post separation dilution is optionally used to improve detection of the mixture of components and the released component of interest. Microfluidic devices and systems suitable for performing such analyses are also provided.
摘要翻译：在分离组分混合物之前，使用上游亲和纯化区域将组分混合物中的一种或多种感兴趣组分结合。检测分离的组分和释放的感兴趣组分提供了感兴趣组分的鉴定。此外，可以使用后分离稀释来改进组分混合物和所释放的感兴趣组分的检测。还提供了适于执行这种分析的微流体装置和系统。

3. 发明申请

US20110306151A1 WESTERN BLOT BY INCORPORATING AN AFFINITY PURIFICATION ZONE 有权
标题翻译：通过加入一个亲属净化区的西部
公开(公告)号：US20110306151A1
公开(公告)日：2011-12-15
申请号：US13216441
申请日：2011-08-24
申请人： Matthew B. Murphy , Robert S. Dubrow
发明人： Matthew B. Murphy , Robert S. Dubrow
IPC分类号： G01N21/00 , G01N30/00
CPC分类号： G01N33/54366
摘要： A mixture of components is flowed through a binding channel region comprising a component-binding moiety, thereby binding at least a portion of a component of interest. The mixture is then flowed through a separation channel region that includes a buffer comprising a detergent, resulting in separated components. Diluent is mixed with the separated components, diluting the detergent, and the separated components are detected. The component of interest is released from the component-binding moiety and flowed through the separation channel region. Diluent is mixed with the released component of interest, diluting the detergent, and the released component of interest is detected.
摘要翻译：组分的混合物流过包含组分结合部分的结合通道区，从而结合目的组分的至少一部分。然后将混合物流过包括包含洗涤剂的缓冲液的分离通道区域，得到分离的组分。将稀释剂与分离的组分混合，稀释洗涤剂，并检测分离的组分。感兴趣的组分从组分结合部分释放并流过分离通道区。将稀释液与所释放的感兴趣组分混合，稀释洗涤剂，并检测释放的感兴趣组分。

4. 发明申请

US20100233030A1 Western Blot by Incorporating an Affinity Purification Zone 有权
标题翻译：通过并入亲和纯化区的Western Blot
公开(公告)号：US20100233030A1
公开(公告)日：2010-09-16
申请号：US12784993
申请日：2010-05-21
申请人： Matthew B. Murphy , Robert S. Dubrow
发明人： Matthew B. Murphy , Robert S. Dubrow
IPC分类号： G01N30/00
CPC分类号： G01N33/54366
摘要： An upstream affinity purification region is used to bind one or more component of interest in a mixture of components prior to separating the mixture of components. Detection of the separated components and a released component of interest provide identification of the component of interest. In addition, post separation dilution is optionally used to improve detection of the mixture of components and the released component of interest. Microfluidic devices and systems suitable for performing such analyses are also provided.
摘要翻译：在分离组分混合物之前，使用上游亲和纯化区域将组分混合物中的一种或多种感兴趣组分结合。检测分离的组分和释放的感兴趣组分提供了感兴趣组分的鉴定。此外，可以使用后分离稀释来改进组分混合物和所释放的感兴趣组分的检测。还提供了适于执行这种分析的微流体装置和系统。

5. 发明授权

US08592141B2 Western blot by incorporating an affinity purification zone 有权
标题翻译：通过掺入亲和纯化区的蛋白质印迹
公开(公告)号：US08592141B2
公开(公告)日：2013-11-26
申请号：US13216441
申请日：2011-08-24
申请人： Matthew B Murphy , Robert S. Dubrow
发明人： Matthew B Murphy , Robert S. Dubrow
IPC分类号： C12Q1/00
CPC分类号： G01N33/54366
摘要： A mixture of components is flowed through a binding channel region comprising a component-binding moiety, thereby binding at least a portion of a component of interest. The mixture is then flowed through a separation channel region that includes a buffer comprising a detergent, resulting in separated components. Diluent is mixed with the separated components, diluting the detergent, and the separated components are detected. The component of interest is released from the component-binding moiety and flowed through the separation channel region. Diluent is mixed with the released component of interest, diluting the detergent, and the released component of interest is detected.
摘要翻译：组分的混合物流过包含组分结合部分的结合通道区，从而结合目的组分的至少一部分。然后将混合物流过包括包含洗涤剂的缓冲液的分离通道区域，得到分离的组分。将稀释剂与分离的组分混合，稀释洗涤剂，并检测分离的组分。感兴趣的组分从组分结合部分释放并流过分离通道区。将稀释液与所释放的感兴趣组分混合，稀释洗涤剂，并检测释放的感兴趣组分。

6. 发明授权

US5215908A Process for recovery and purification of chymosin 失效
标题翻译：凝乳酶的回收和纯化方法
公开(公告)号：US5215908A
公开(公告)日：1993-06-01
申请号：US869838
申请日：1992-04-16
申请人： Henry G. Heinsohn , Matthew B. Murphy
发明人： Henry G. Heinsohn , Matthew B. Murphy
IPC分类号： C12N9/64
CPC分类号： C12N9/6481 , Y10S435/815
摘要： This invention provides a method of recovering and purifying chymosin from fermentation used for microbial production of chymosin. The fermentation mixture is filtered to provide a filtrate containing the chymosin. The filtrate is first prepared by either adjusting it to a low pH, such as about 2 and/or adjusting the salt concentration of the filtrate to at least about 2M. The aqueous filtrate mixture is contacted with a phenyl-sepharose resin, which selectively binds the chymosin from the filtrate. The filtrate remaining after the contact with the phenyl-sepharose resin is discarded, and the phenyl-sepharose resin column is eluted with water or dilute salt solution to obtain substantially pure chymosin in a single elution step.
摘要翻译：本发明提供从用于微生物生产凝乳酶的发酵中回收和纯化凝乳酶的方法。将发酵混合物过滤以提供含有凝乳酶的滤液。首先通过将滤液调节至低pH（如约2）和/或将滤液的盐浓度调节至至少约2M来制备滤液。将含水滤液混合物与苯基 - 琼脂糖树脂接触，苯基 - 琼脂糖树脂从滤液中选择性地结合凝乳酶。在与苯基 - 琼脂糖树脂接触后残留的滤液被丢弃，苯基 - 琼脂糖树脂柱用水或稀盐溶液洗脱，以在单一洗脱步骤中获得基本上纯的凝乳酶。

7. 发明授权

US08007738B2 Western blot by incorporating an affinity purification zone 有权
标题翻译：通过掺入亲和纯化区的蛋白质印迹
公开(公告)号：US08007738B2
公开(公告)日：2011-08-30
申请号：US12784993
申请日：2010-05-21
申请人： Matthew B. Murphy , Robert S. Dubrow
发明人： Matthew B. Murphy , Robert S. Dubrow
IPC分类号： B01L3/00
CPC分类号： G01N33/54366
摘要： An upstream affinity purification region is used to bind one or more component of interest in a mixture of components prior to separating the mixture of components. Detection of the separated components and a released component of interest provide identification of the component of interest. In addition, post separation dilution is optionally used to improve detection of the mixture of components and the released component of interest. Microfluidic devices and systems suitable for performing such analyses are also provided.
摘要翻译：在分离组分混合物之前，使用上游亲和纯化区域将组分混合物中的一种或多种感兴趣组分结合。检测分离的组分和释放的感兴趣组分提供了感兴趣组分的鉴定。此外，可以使用后分离稀释来改进组分混合物和所释放的感兴趣组分的检测。还提供了适于执行这种分析的微流体装置和系统。

8. 发明授权

US07754150B2 Microfluidic analytic detection assays, devices, and integrated systems 有权
标题翻译：微流控分析检测，装置和集成系统
公开(公告)号：US07754150B2
公开(公告)日：2010-07-13
申请号：US10613220
申请日：2003-07-02
申请人： H. Garrett Wada , Matthew B. Murphy
发明人： H. Garrett Wada , Matthew B. Murphy
IPC分类号： G01N33/00 , G01N35/00 , C12Q1/00
CPC分类号： G01N33/543 , Y10T436/11
摘要： Methods of detecting a component of interest, such as a protein, in a microfluidic system are provided. The methods include the use of a component-binding moiety specific to the component of interest, such as an antibody, to detect the component of interest. Also included are microfluidic devices and integrated systems for performing such assays, including devices utilizing flowable or fixed particle sets.
摘要翻译：提供了在微流体系统中检测感兴趣的组分，例如蛋白质的方法。所述方法包括使用特异性的组分结合部分（例如抗体）来检测感兴趣的组分。还包括用于进行这种测定的微流体装置和集成系统，包括利用可流动或固定的颗粒组的装置。

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