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    • 3. 发明授权
    • Preparation of a hydrophobic polymer matrix containing immobilized
enzymes
    • 制备含有固定化酶的疏水性聚合物基质
    • US5928918A
    • 1999-07-27
    • US843295
    • 1997-04-14
    • Helmut OffenbacherBernhard SchaffarMassoud Ghahramani
    • Helmut OffenbacherBernhard SchaffarMassoud Ghahramani
    • G01N27/327C12N11/04C12N11/08C12N11/18C12Q1/00C12M1/34C12Q1/26C12Q1/34
    • C12Q1/003C12N11/04C12N11/08C12N11/18C12Q1/001C12Q1/005Y10S435/817
    • Biological components such as enzymes are immobilized in a three-dimensional cross-linked hydrophobic polymer. An enzyme is mixed with an aqueous solvent and a non-crosslinked prepolymer having an essentially nonpolar main chain with attached polar hydrophilic groups and cross-linking groups. The resultant mixture is exposed to cross-linking temperatures to react the prepolymer via the cross-linking groups without additional catalyst or cross-linking agents, and the aqueous solvent is evaporated to form a three-dimensional cross-linked hydrophobic polymer matrix containing the enzyme. The prepolymer can be an oil alkyl resin containing the main chain with attached polar hydrophilic groups and cross-linking groups. The oil alkyl resin is cross-linked by autoxidation, and aqueous solvent is evaporated to form the polymer matrix. A biosensor is prepared having the enzyme-containing polymer matrix on the surface of a supporting layer which contains cross-linking groups that improve adhesion between the supporting layer and the polymer matrix. Catalytic particles or charge conducting particles may be in the polymer matrix, and multiple polymer matrixes with different enzymes may be formed.
    • 诸如酶的生物成分被固定在三维交联的疏水性聚合物中。 将酶与水性溶剂和具有基本上非极性主链的非交联预聚物混合,具有连接的极性亲水基团和交联基团。 将所得混合物暴露于交联温度以通过交联基团使预聚物反应而无需另外的催化剂或交联剂,并将水性溶剂蒸发以形成含有该酶的三维交联疏水性聚合物基质 。 预聚物可以是含有带有极性亲水基团和交联基团的主链的油状烷基树脂。 油状烷基树脂通过自氧化进行交联,并蒸发水性溶剂以形成聚合物基质。 制备生物传感器,其在支撑层的表面上含有含酶聚合物基质,其含有改善支持层和聚合物基质之间的粘附性的交联基团。 催化颗粒或电荷导电颗粒可以在聚合物基质中,并且可以形成具有不同酶的多个聚合物基质。