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    • 8. 发明申请
    • Novel transferase and amylase, process for producing the enzymes, use thereof, and gene coding for the same
    • 新型转移酶和淀粉酶,用于生产酶的方法,其用途及其编码基因
    • US20070087426A1
    • 2007-04-19
    • US11526632
    • 2006-09-26
    • Masaru KatoYutaka MiuraMasako KettokuAkihiro IwamatsuKazuo KobayashiToshihiro Komeda
    • Masaru KatoYutaka MiuraMasako KettokuAkihiro IwamatsuKazuo KobayashiToshihiro Komeda
    • C12N9/10C07H21/04
    • C12N9/2411C12N9/1048C12N9/2408C12N9/2414C12N9/2417C12P19/14
    • The invention provides a novel transferase that acts on a saccharide, as a substrate, composed of at least three sugar units wherein at least three glucose residues on the reducing end are linked α-1,4 so as to transfer the α-1,4 lingages to a α-1, α-1 linkages; a process for producing the transferase; a gene coding for the same; and a process for producing an oligosaccharide by using the same. Also provided are a novel amylase that has a principal activity of acting on a saccharide, as a substrate, composed of at least three sugar units wherein at least three sugar units on the reducing end side are glucose units and the linkage between the first and the second glucose units is α-1, α-1 while the linkage between the second and the third glucose units is α-1,4 so as to liberate α, α-trehalose by hydrolyzing the α-1,4 linkage and another activity of hydrolyzing the α-1,4 linkage within the molecular chain of the substrate and that liberates disaccharides and/or monosaccharides as the principal final products; a process for producing the amylase; a gene coding for the same; and a process for producing α, α-trehalose by using a combination of the transferase and the amylase.
    • 本发明提供了一种新的转移酶,其作用为由至少三个糖单元组成的作为底物的糖,其中还原端上的至少三个葡萄糖残基与α-1,4连接以转移α-1,4 属于α-1,α-1键; 生产转移酶的方法; 编码相同的基因; 以及使用该寡糖的方法。 还提供了一种新的淀粉酶,其具有作用于由至少三个糖单元组成的糖类作为底物的主要活性,其中还原端侧的至少三个糖单元是葡萄糖单元,第一和第 第二葡萄糖单位是α-1,α-1,而第二和第三葡萄糖单元之间的连接是α-1,4,以便通过水解α-1,4键和另一种活性的α-1,4键来释放α,α-海藻糖 水解基质分子链内的α-1,4键,释放出二糖和/或单糖作为主要的最终产物; 淀粉酶的制造方法; 编码相同的基因; 以及通过使用转移酶和淀粉酶的组合产生α,α-海藻糖的方法。