专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

US20130041145A1 METHOD FOR ISOLATING RNA FROM WHOLE BLOOD SAMPLES 审中-公开
标题翻译：从全血样品中分离RNA的方法
公开(公告)号：US20130041145A1
公开(公告)日：2013-02-14
申请号：US13572072
申请日：2012-08-10
申请人： Christoph KIRSCH , Claudia Beyard , Markus Meusel , Thomas Zinn , Carolin Wagner , Klaus Moeller
发明人： Christoph KIRSCH , Claudia Beyard , Markus Meusel , Thomas Zinn , Carolin Wagner , Klaus Moeller
IPC分类号： C07H1/08 , C12N9/50
CPC分类号： C12N15/1006 , C12N1/06 , C12N15/1003
摘要： The present invention relates to a method for isolating RNA from a whole blood sample, comprising the steps bringing the sample into contact with an aqueous lysis solution containing at least one lysis substance in a concentration of 1.5 mol/1 to 7 mol/1 and at least one detergent, simultaneously or subsequently adding a proteinase, in particular proteinase K, then incubating the solution for at least partial enzymatic digestion and lysis of the sample so that a lysate is obtained, and isolating the RNA from the lysate.
摘要翻译：本发明涉及从全血样品中分离RNA的方法，包括使样品与含有至少一种浓度为1.5mol / l至7mol / l的裂解物质的水溶液溶液接触的步骤，以及至少一种洗涤剂，同时或随后加入蛋白酶，特别是蛋白酶K，然后将溶液温育至少部分酶消化和裂解样品，从而获得裂解物，并从裂解物中分离RNA。

2. 发明授权

US09845488B2 Method for the deparaffinisation of biological specimens 有权
公开(公告)号：US09845488B2
公开(公告)日：2017-12-19
申请号：US12827210
申请日：2010-06-30
申请人： Christoph Kirsch , Claudia Beyard , Markus Meusel , Klaus Moeller
发明人： Christoph Kirsch , Claudia Beyard , Markus Meusel , Klaus Moeller
IPC分类号： C07H1/08 , C12Q1/68 , C12N15/10
CPC分类号： C12Q1/6806 , C12N15/1003
摘要： A method for isolating biomolecules from a fixed biological specimen embedded in paraffin, comprises the steps of bringing the specimen into contact a) with a solvent non-miscible with water and dissolving the paraffin in this solvent such as to form a liquid organic phase, and b) with an aqueous solution of at least one lysis substance and/or with water and at least one lysis substance such as to form an aqueous phase; isolation of the biomolecules from the aqueous phase. The invention further relates to a corresponding kit as well as to the use of such a kit for isolating biomolecules.

3. 发明申请

US20110003369A1 METHOD FOR THE DEPARAFFINISATION OF BIOLOGICAL SPECIMENS 有权
标题翻译：生物样本减毒方法
公开(公告)号：US20110003369A1
公开(公告)日：2011-01-06
申请号：US12827210
申请日：2010-06-30
申请人： Christoph KIRSCH , Claudia Beyard , Markus Meusel , Klaus Moeller
发明人： Christoph KIRSCH , Claudia Beyard , Markus Meusel , Klaus Moeller
IPC分类号： C07H1/08
CPC分类号： C12Q1/6806 , C12N15/1003
摘要： A method for isolating biomolecules from a fixed biological specimen embedded in paraffin, comprises the steps of bringing the specimen into contact a) with a solvent non-miscible with water and dissolving the paraffin in this solvent such as to form a liquid organic phase, and b) with an aqueous solution of at least one lysis substance and/or with water and at least one lysis substance such as to form an aqueous phase; isolation of the biomolecules from the aqueous phase. The invention further relates to a corresponding kit as well as to the use of such a kit for isolating biomolecules.
摘要翻译：从石蜡包埋的固定生物样品中分离生物分子的方法包括以下步骤：使样品与不与水混溶的溶剂接触，并将石蜡溶解在该溶剂中以形成液态有机相， b）与至少一种裂解物质和/或与水和至少一种裂解物质的水溶液形成水相; 从水相中分离生物分子。本发明还涉及相应的试剂盒以及这种试剂盒用于分离生物分子的用途。

4. 发明授权

US06686208B2 Device and method for carrying out fluoresence immunotests 失效
标题翻译：用于进行荧光免疫测定的装置和方法
公开(公告)号：US06686208B2
公开(公告)日：2004-02-03
申请号：US10212954
申请日：2002-08-06
申请人： Markus Meusel , Dieter Trau , Andreas Katerkamp
发明人： Markus Meusel , Dieter Trau , Andreas Katerkamp
IPC分类号： G01N3353
CPC分类号： G01N21/648 , G01N21/6428 , G01N33/54373 , Y10S435/808 , Y10S435/968 , Y10S435/969 , Y10S436/805 , Y10S436/807
摘要： The invention relates to devices and methods for carrying out quantitative fluorescence immunoassays using evanescent field excitation. Light from at least one light source is directed onto the boundary between two media which have differing refractive indices. The light source emits practically monochromatic light with a wavelength suitable for exciting a marking substance. The light is directed onto a boundary surface disposed between an optically transparent base plate, the refractive index of which is greater than that of the material above the boundary surface, and a receiving region for the sample. The receiving region is covered with a covering plate on the side disposed opposite the base plate, there being arranged between the base plate and covering plate at least one functional layer. A detector for detecting the fluorescent light is disposed on the same side of the base plate as the light source.
摘要翻译：本发明涉及使用ev逝场激发进行定量荧光免疫测定的装置和方法。来自至少一个光源的光被引导到具有不同折射率的两个介质之间的边界上。光源发射具有适于激发标记物质的波长的实际单色光。光被引导到设置在光学透明基板之间的边界表面，其折射率大于边界表面上方的材料的折射率，以及用于样品的接收区域。接收区域在与基板相对设置的一侧覆盖有覆盖板，在基板和覆盖板之间布置有至少一个功能层。用于检测荧光的检测器设置在与光源相同的基板的一侧。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式