专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明授权

US07585625B2 Storing and detecting nucleic acid administered to a solid carrier 有权
标题翻译：存储和检测施用于固体载体的核酸
公开(公告)号：US07585625B2
公开(公告)日：2009-09-08
申请号：US10817164
申请日：2004-04-01
申请人： Esther Regina de Rooij , Marinus Petrus de Baar
发明人： Esther Regina de Rooij , Marinus Petrus de Baar
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6806 , C12Q1/703 , C12Q2545/114 , C12Q2565/518
摘要： The invention discloses a method for detecting a nucleic acid of interest in at least one sample comprising administering the sample to a solid carrier capable of at least in part absorbing the sample, drying the carrier, providing at least a representative part of the carrier to a nucleic acid isolation solution so that a representative amount of the nucleic acid is extracted from the carrier, and detecting the representative amount of the nucleic acid. With a method of the invention, a sample, such as a body fluid sample, is stabilized in such a way that it can be shipped from the site of taking (for instance local hospital or lab in a less-developed country) and be sent to a service testing laboratory elsewhere in the world by normal logistics means. At least 100 μl or at least 250 μl of sample is administered to the carrier in order to detect low titers of nucleic acid of interest. A kit for detecting, identifying and/or quantifying a nucleic acid of interest in a sample, comprising a solid carrier capable of at least in part absorbing the sample, and a nucleic acid isolation solution, is also described.
摘要翻译：本发明公开了一种用于在至少一个样品中检测感兴趣的核酸的方法，包括将样品施用于至少部分吸收样品的固体载体，干燥载体，至少提供载体的代表性部分至核酸分离溶液，使得从载体中提取代表量的核酸，并检测核酸的代表量。通过本发明的方法，使诸如体液样品的样品稳定，使得其可以从服用部位（例如在较不发达国家的当地医院或实验室）运送并被发送通过正常的物流手段到世界其他地方的服务测试实验室。为了检测目的核酸的低滴度，向载体施用至少100个或至少250个样品的样品。还描述了用于检测，鉴定和/或定量样品中目标核酸的试剂盒，其包含至少部分吸收样品的固体载体和核酸分离溶液。

2. 发明授权

US06750014B2 Reducing background in hybridization reactions 失效
标题翻译：减少杂交反应的背景
公开(公告)号：US06750014B2
公开(公告)日：2004-06-15
申请号：US09785881
申请日：2001-02-16
申请人： Marinus Petrus de Baar , Eveline Catherina A. C. Timmermans , Bob van Gemen
发明人： Marinus Petrus de Baar , Eveline Catherina A. C. Timmermans , Bob van Gemen
IPC分类号： C12Q168
CPC分类号： C12Q1/6827 , C12Q1/6832
摘要： The invention provides a method for reducing background in hybridization reactions of nucleic acids involving at least two homologous probes, wherein at least one of the probes is non-linear, or two homologous target sequences and a non-linear probe. Background is reduced by introducing an intended mismatch with a target sequence in at least one of the probes. The presence of the mismatch reduces the specificity of probes not entirely complementary to a target sequence to such an extent that the background signal is reduced. A set of mixed homologous probes, wherein at least one of the probes is non-linear, comprising such specific mismatch is also provided. The set can be used for the detection of variants of a family of nucleic acids, for instance a number of HIV variants. The invention also provides kits for carrying out the methods according to the invention.
摘要翻译：本发明提供了一种减少涉及至少两种同源探针的核酸的杂交反应背景的方法，其中至少一种探针是非线性的，或两个同源靶序列和非线性探针。通过在至少一个探针中引入目标序列的预期错配来减少背景。不匹配的存在降低了探针与靶序列不完全互补的特异性，使得背景信号降低的程度。还提供了一组混合同源探针，其中至少一个探针是非线性的，包括这种特异性错配。该组可用于检测核酸家族的变体，例如许多HIV变体。本发明还提供了用于实施根据本发明的方法的试剂盒。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式