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1. 发明授权

US06319692B1 Methods for transferring gene into chromosome 有权
标题翻译：将基因转入染色体的方法
公开(公告)号：US06319692B1
公开(公告)日：2001-11-20
申请号：US09202893
申请日：1998-12-22
申请人： Mariko Kadota , Mayumi Kiwaki , Saeko Sawaki , Yukio Shirasawa , Harue Sone , Tomoyuki Sako
发明人： Mariko Kadota , Mayumi Kiwaki , Saeko Sawaki , Yukio Shirasawa , Harue Sone , Tomoyuki Sako
IPC分类号： C12P1934
CPC分类号： C12N15/907 , C12N15/746 , C12N15/90
摘要： Methods for transferring a foreign gene into a host chromosome by integrating the foreign gene into a vector by utilizing a lysogenic phage, for the purpose of deleting unnecessary genes derived from the vector, except for the foreign gene. One method comprises (a) preparing a vector with a lysogenic phage integration site (such as an attP site) arranged between a first partial sequence of the objective foreign gene to be transferred, but lacking one terminal region thereof and a second partial sequence of the gene, but lacking the other terminal region, the second partial sequence having an overlapping region with a portion of the first partial sequence; (b) integrating the vector obtained in step (a) into the host chromosome; and (c) screening from among the recombinants obtained in step (b) a recombinant from which unnecessary genes originating in the vector have been deleted owing to the homologous recombination mechanism functioning in the overlapping region between the first and second partial sequences.
摘要翻译：通过利用溶源性噬菌体将外源基因整合到载体中，为了除去外来基因以外的来自载体的不必要的基因，将外源基因转移到宿主染色体中的方法。一种方法包括（a）制备载体，该载体具有排列在要转移的目标外源基因的第一部分序列之间，但缺少其一个末端区域和第二部分序列的溶源性噬菌体整合位点（例如attP位点）基因，但缺少另一个末端区域，第二部分序列与第一部分序列的一部分具有重叠区域; （b）将步骤（a）中获得的载体整合到宿主染色体中; 和（c）从步骤（b）中获得的重组体中筛选来自该载体的不必要的基因由于第一和第二部分序列之间的重叠区域中的同源重组机制起作用而被缺失的重组体。

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