专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明授权

US08445283B2 Methods for producing biological substances in enzyme-deficient mutants of Aspergillus niger 有权
标题翻译：在黑曲霉酶缺陷突变体中生产生物物质的方法
公开(公告)号：US08445283B2
公开(公告)日：2013-05-21
申请号：US12823046
申请日：2010-06-24
申请人： Mariah Connelly , Howard Brody
发明人： Mariah Connelly , Howard Brody
IPC分类号： C12N15/74 , C12N1/14
CPC分类号： C07K14/37 , C07K14/38 , C07K14/40 , C12N9/00 , C12N15/52 , C12P1/02 , C12P21/02
摘要： The present invention relates to methods of producing a heterologous biological substance, comprising: (a) cultivating a mutant of a parent Aspergillus niger strain in a medium suitable for the production of the heterologous biological substance, wherein (i) the mutant strain comprises a first nucleotide sequence encoding the heterologous biological substance and one or more second nucleotide sequences comprising a modification of glaA and at least one of the genes selected from the group consisting of asa, amyA, amyB, prtT, and oah, and (ii) the mutant strain is deficient in the production of glucoamylase and at least one enzyme selected from the group consisting of acid stable alpha-amylase, neutral alpha-amylase A, and neutral alpha-amylase B, protease, and oxalic acid hydrolase compared to the parent Aspergillus niger strain when cultivated under identical conditions; and (b) recovering the heterologous biological substance from the cultivation medium. The present invention also relates to enzyme-deficient mutants of Aspergillus niger strains and methods for producing such mutants.
摘要翻译：本发明涉及产生异源生物物质的方法，包括：（a）在适于生产异源生物物质的培养基中培养母本黑曲霉菌株的突变体，其中（i）突变株包含第一编码异源生物物质的核苷酸序列和一个或多个第二核苷酸序列，其包含glaA的修饰和至少一种选自asa，amyA，amyB，prtT和oah的基因，以及（ii）突变株与母本黑曲霉菌株相比，生产葡糖淀粉酶和至少一种选自酸稳定的α-淀粉酶，中性α-淀粉酶A和中性α-淀粉酶B，蛋白酶和草酸水解酶的酶不足在相同的条件下耕种; 和（b）从培养基中回收异源生物物质。本发明还涉及黑曲霉菌株的酶缺陷型突变体及其生产方法。

2. 发明申请

US20080044858A1 METHODS FOR PRODUCING BIOLOGICAL SUBSTANCES IN ENZYME-DEFICIENT MUTANTS OF ASPERGILLUS NIGER 有权
标题翻译：生物化学物质生物物质的生物学方法在苦参碱的缺乏突变体
公开(公告)号：US20080044858A1
公开(公告)日：2008-02-21
申请号：US11837279
申请日：2007-08-10
申请人： Mariah Connelly , Howard Brody
发明人： Mariah Connelly , Howard Brody
IPC分类号： C12N1/00 , C12P21/04
CPC分类号： C07K14/37 , C07K14/38 , C07K14/40 , C12N9/00 , C12N15/52 , C12P1/02 , C12P21/02
摘要： The present invention relates to methods of producing a heterologous biological substance, comprising: (a) cultivating a mutant of a parent Aspergillus niger strain in a medium suitable for the production of the heterologous biological substance, wherein (i) the mutant strain comprises a first nucleotide sequence encoding the heterologous biological substance and one or more second nucleotide sequences comprising a modification of glaA and at least one of the genes selected from the group consisting of asa, amyA, amyB, prtT, and oah, and (ii) the mutant strain is deficient in the production of glucoamylase and at least one enzyme selected from the group consisting of acid stable alpha-amylase, neutral alpha-amylase A, and neutral alpha-amylase B, protease, and oxalic acid hydrolase compared to the parent Aspergillus niger strain when cultivated under identical conditions: and (b) recovering the heterologous biological substance from the cultivation medium. The present invention also relates to enzyme-deficient mutants of Aspergillus niger strains and methods for producing such mutants.
摘要翻译：本发明涉及产生异源生物物质的方法，包括：（a）在适于生产异源生物物质的培养基中培养母本黑曲霉菌株的突变体，其中（i）突变株包含第一编码异源生物物质的核苷酸序列和一个或多个第二核苷酸序列，其包含glaA的修饰和至少一种选自asa，amyA，amyB，prtT和oah的基因，以及（ii）突变株与母本黑曲霉菌株相比，生产葡糖淀粉酶和至少一种选自酸稳定的α-淀粉酶，中性α-淀粉酶A和中性α-淀粉酶B，蛋白酶和草酸水解酶的酶不足当在相同条件下培养时：（b）从培养基中回收异源生物物质。本发明还涉及黑曲霉菌株的酶缺陷型突变体及其生产方法。

3. 发明申请

US20100323448A1 Methods For Producing Biological Substances In Enzyme-Deficient Mutants Of Aspergillus niger 有权
标题翻译：在黑曲霉的酶缺陷突变体中生产生物物质的方法
公开(公告)号：US20100323448A1
公开(公告)日：2010-12-23
申请号：US12823046
申请日：2010-06-24
申请人： Mariah Connelly , Howard Brody
发明人： Mariah Connelly , Howard Brody
IPC分类号： C12N15/74 , C12N1/15
CPC分类号： C07K14/37 , C07K14/38 , C07K14/40 , C12N9/00 , C12N15/52 , C12P1/02 , C12P21/02
摘要： The present invention relates to methods of producing a heterologous biological substance, comprising: (a) cultivating a mutant of a parent Aspergillus niger strain in a medium suitable for the production of the heterologous biological substance, wherein (i) the mutant strain comprises a first nucleotide sequence encoding the heterologous biological substance and one or more second nucleotide sequences comprising a modification of glaA and at least one of the genes selected from the group consisting of asa, amyA, amyB, prtT, and oah, and (ii) the mutant strain is deficient in the production of glucoamylase and at least one enzyme selected from the group consisting of acid stable alpha-amylase, neutral alpha-amylase A, and neutral alpha-amylase B, protease, and oxalic acid hydrolase compared to the parent Aspergillus niger strain when cultivated under identical conditions; and (b) recovering the heterologous biological substance from the cultivation medium. The present invention also relates to enzyme-deficient mutants of Aspergillus niger strains and methods for producing such mutants.
摘要翻译：本发明涉及产生异源生物物质的方法，包括：（a）在适于生产异源生物物质的培养基中培养母本黑曲霉菌株的突变体，其中（i）突变株包含第一编码异源生物物质的核苷酸序列和一个或多个第二核苷酸序列，其包含glaA的修饰和至少一种选自asa，amyA，amyB，prtT和oah的基因，以及（ii）突变株与母本黑曲霉菌株相比，生产葡糖淀粉酶和至少一种选自酸稳定的α-淀粉酶，中性α-淀粉酶A和中性α-淀粉酶B，蛋白酶和草酸水解酶的酶不足在相同的条件下耕种; 和（b）从培养基中回收异源生物物质。本发明还涉及黑曲霉菌株的酶缺陷型突变体及其生产方法。

4. 发明授权

US07771971B2 Methods for producing biological substances in enzyme-deficient mutants of Aspergillus niger 有权
标题翻译：在黑曲霉酶缺陷突变体中生产生物物质的方法
公开(公告)号：US07771971B2
公开(公告)日：2010-08-10
申请号：US11837279
申请日：2007-08-10
申请人： Mariah Connelly , Howard Brody
发明人： Mariah Connelly , Howard Brody
IPC分类号： C12P21/04
CPC分类号： C07K14/37 , C07K14/38 , C07K14/40 , C12N9/00 , C12N15/52 , C12P1/02 , C12P21/02
摘要： The present invention relates to methods of producing a heterologous biological substance, comprising: (a) cultivating a mutant of a parent Aspergillus niger strain in a medium suitable for the production of the heterologous biological substance, wherein (i) the mutant strain comprises a first nucleotide sequence encoding the heterologous biological substance and one or more second nucleotide sequences comprising a modification of glaA and at least one of the genes selected from the group consisting of asa, amyA, amyB, prtT, and oah, and (ii) the mutant strain is deficient in the production of glucoamylase and at least one enzyme selected from the group consisting of acid stable alpha-amylase, neutral alpha-amylase A, and neutral alpha-amylase B, protease, and oxalic acid hydrolase compared to the parent Aspergillus niger strain when cultivated under identical conditions; and (b) recovering the heterologous biological substance from the cultivation medium. The present invention also relates to enzyme-deficient mutants of Aspergillus niger strains and methods for producing such mutants.
摘要翻译：本发明涉及产生异源生物物质的方法，包括：（a）在适于生产异源生物物质的培养基中培养母本黑曲霉菌株的突变体，其中（i）突变株包含第一编码异源生物物质的核苷酸序列和一个或多个第二核苷酸序列，其包含glaA的修饰和至少一种选自asa，amyA，amyB，prtT和oah的基因，以及（ii）突变株与母本黑曲霉菌株相比，生产葡糖淀粉酶和至少一种选自酸稳定的α-淀粉酶，中性α-淀粉酶A和中性α-淀粉酶B，蛋白酶和草酸水解酶的酶不足在相同的条件下耕种; 和（b）从培养基中回收异源生物物质。本发明还涉及黑曲霉菌株的酶缺陷型突变体及其生产方法。

5. 发明授权

US07303877B2 Methods for producing biological substances in enzyme-deficient mutants of Aspergillus 有权
标题翻译：在曲霉菌酶缺陷突变体中生产生物物质的方法
公开(公告)号：US07303877B2
公开(公告)日：2007-12-04
申请号：US10815495
申请日：2004-03-31
申请人： Mariah Connelly , Howard Brody
发明人： Mariah Connelly , Howard Brody
IPC分类号： C12Q1/68
CPC分类号： C07K14/37 , C07K14/38 , C07K14/40 , C12N9/00 , C12N15/52 , C12P1/02 , C12P21/02
摘要： The present invention relates to methods of producing a heterologous biological substance, comprising: (a) cultivating a mutant of a parent Aspergillus niger strain in a medium suitable for the production of the heterologous biological substance, wherein (i) the mutant strain comprises a first nucleotide sequence encoding the heterologous biological substance and one or more second nucleotide sequences comprising a modification of glaA and at least one of the genes selected from the group consisting of asa, amyA, amyB, prtT, and oah, and (ii) the mutant strain is deficient in the production of glucoamylase and at least one enzyme selected from the group consisting of acid stable alpha-amylase, neutral alpha-amylase A, and neutral alpha-amylase B, protease, and oxalic acid hydrolase compared to the parent Aspergillus niger strain when cultivated under identical conditions; and (b) recovering the heterologous biological substance from the cultivation medium. The present invention also relates to enzyme-deficient mutants of Aspergillus niger strains and methods for producing such mutants.
摘要翻译：本发明涉及产生异源生物物质的方法，包括：（a）在适于生产异源生物物质的培养基中培养母本黑曲霉菌株的突变体，其中（i）突变株包含第一编码异源生物物质的核苷酸序列和一个或多个第二核苷酸序列，其包含glaA的修饰和至少一种选自asa，amyA，amyB，prtT和oah的基因，以及（ii）突变株与母本黑曲霉菌株相比，生产葡糖淀粉酶和至少一种选自酸稳定的α-淀粉酶，中性α-淀粉酶A和中性α-淀粉酶B，蛋白酶和草酸水解酶的酶不足在相同的条件下耕种; 和（b）从培养基中回收异源生物物质。本发明还涉及黑曲霉菌株的酶缺陷型突变体及其生产方法。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式