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    • 5. 发明授权
    • Immobilization of pseudomonas lipase on surfaces for oil removal
    • 固定假单孢菌脂肪酶在表面去除油
    • US06265191B1
    • 2001-07-24
    • US08110341
    • 1993-08-20
    • Eugene A. MizusawaSusan A. AndersonMaha Y. El-SayedDaniel R. LeiskeRichard J. WiersemaChihae Yang
    • Eugene A. MizusawaSusan A. AndersonMaha Y. El-SayedDaniel R. LeiskeRichard J. WiersemaChihae Yang
    • C12N1102
    • C11D3/38627D06M16/003Y10S435/877
    • Lipase is immobilized on surfaces to facilitate oil removal from the surfaces and to alter wettability of the surfaces. The lipase is isolatable from a Pseudomonas organism such as Pseudomonas putida ATCC 53552 or from an organism expressing a coding region found in or cloned from the Pseudomonas. A particularly preferred lipase has a molecular weight of about 30 to 35 kd and is resolvable as a single band by SDS gel electrophoresis. Lipase sorbed on fabric forms a fabric-lipase complex for oil stain removal. The lipase may be sorbed on fabric before or after an oil stain, and the lipase is active to hydrolyze an oil stain on dry fabric or fabric in laundering solutions. The sorbed lipase has enhanced stability to denaturation by surfactants and to heat deactivation, is resistant to removal from fabric during laundering, retains substantial activity after drying fabric at an elevated temperature, and retains activity during fabric storage or wear. Redeposition of oil and oil hydrolysis by-products during laundering of fabric is retarded by the lipase. Oil hydrolysis by-products are removable during laundering of fabric at a basic pH or in the presence of a surfactant.
    • 将脂肪酶固定在表面上以促进从表面除油并改变表面的润湿性。 所述脂肪酶可从假单胞菌(Pseudomonas)生物体如恶臭假单胞菌ATCC 53552或表达在假单胞菌中发现或克隆的编码区的生物体分离。 特别优选的脂肪酶具有约30至35kd的分子量,并且可通过SDS凝胶电泳作为单一条带分解。 在织物上吸附的脂肪酶形成用于除油的织物 - 脂肪酶复合物。 脂肪酶可以在油渍之前或之后在织物上吸附,并且脂肪酶在洗涤溶液中活性水解干织物或织物上的油渍。 吸附的脂肪酶具有增强的表面活性剂变性和热失活的稳定性,在洗涤期间耐织物除去后,在高温下干燥织物后保留显着的活性,并且在织物储存或磨损期间保持活性。 在织物洗涤过程中油和水解副产物的重新沉积被脂肪酶阻滞。 在碱性pH下或在表面活性剂存在下,织物洗涤期间油水解副产物是可除去的。