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1. 发明申请

WO2007070726A2 A GENE SYNTHESIS KIT 审中-公开
标题翻译：基因合成工具包
公开(公告)号：WO2007070726A2
公开(公告)日：2007-06-21
申请号：PCT/US2006/048550
申请日：2006-12-18
申请人： GENE ORACLE, INC. , MITCHELL, Kenneth, W. , COX, Anthony, R.
发明人： MITCHELL, Kenneth, W. , COX, Anthony, R.
IPC分类号： G06F19/22
CPC分类号： C12N15/10 , C12N15/1089 , G06F19/20 , G06F19/22
摘要： This disclosure is directed to the field of polynucleotide synthesis, and the embodiments taught herein are generally directed to a kit for use in the design of a desired polynucleotide from information obtained from a polypeptide or another polynucleotide, the generation of a custom set of oligonucleotides that complement the design, and the ordering of the custom set of oligonucleotides. The invention includes systems and methods for producing a desired polynucleotide using the kit.
摘要翻译：本公开涉及多核苷酸合成领域，并且本文教导的实施方案通常涉及用于从多肽或另一多核苷酸获得的信息设计所需多核苷酸的试剂盒，产生定制的寡核苷酸组补充了设计，以及定制的寡核苷酸组的排序。本发明包括使用试剂盒生产所需多核苷酸的系统和方法。

2. 发明申请

WO1996038517A1 A PROCESS FOR DEMETALIZING A FOSSIL FUEL 审中-公开
标题翻译：一种FOSSIL燃料的去除方法
公开(公告)号：WO1996038517A1
公开(公告)日：1996-12-05
申请号：PCT/US1996008371
申请日：1996-06-03
申请人： ENERGY BIOSYSTEMS CORPORATION , XU, Guo-Wei , MITCHELL, Kenneth, W. , MONTICELLO, Daniel, J.
发明人： ENERGY BIOSYSTEMS CORPORATION
IPC分类号： C10G32/00
CPC分类号： C10G1/00 , C10G32/00
摘要： The invention relates to a method for removing metals from a fossil fuel comprising the steps of contacting the fossil fuel with a biocatalyst selected from the group consisting of an enzyme which degrades porphyrin molecules under conditions suitable for the removal of the metals from the fossil fuel; and separating the metals from the fossil fuel. Preferred embodiments of the biocatalyst include heme oxygenase and cytochrome C reductase, such as cytochrome C reductase from Bacillus megaterium, Catharanthus roseuse, Escherichia coli, animal cells, plant cells or yeast cells. The cyctochrome C reductase can be contacted with the fossil fuel in an aqueous medium as a substantially cell-free preparation or cell preparation. In one embodiment of the invention, the metals are recovered from the resulting metal containing stream.
摘要翻译：本发明涉及一种从化石燃料中去除金属的方法，包括以下步骤：使化石燃料与生物催化剂接触，所述生物催化剂选自于在适于从化石燃料中除去金属的条件下降解卟啉分子的酶; 并从化石燃料中分离金属。生物催化剂的优选实施方案包括血红素加氧酶和细胞色素C还原酶，例如来自巨大芽孢杆菌的细胞色素C还原酶，长春花，大肠杆菌，动物细胞，植物细胞或酵母细胞。细胞色素C还原酶可以在水性介质中与化石燃料接触，作为基本上无细胞的制剂或细胞制剂。在本发明的一个实施方案中，从所得到的含金属流中回收金属。

3. 发明申请

WO2006047589A3 IMPROVED ALANINE 2,3-AMINOMUTASES AND RELATED POLYNUCLEOTIDES 审中-公开
公开(公告)号：WO2006047589A3
公开(公告)日：2006-05-04
申请号：PCT/US2005/038552
申请日：2005-10-25
申请人： CODEXIS, INC. , CHATTERJEE, Ranjini , MITCHELL, Kenneth, W. , LOUIE, Susan, Y. , FOX, Richard, J. , CHEN, Michelle
发明人： CHATTERJEE, Ranjini , MITCHELL, Kenneth, W. , LOUIE, Susan, Y. , FOX, Richard, J. , CHEN, Michelle
IPC分类号： C12N9/90 , C12N15/10
摘要： The present invention is directed to polypeptides that have enhanced alanine 2,3-aminomutase (AAM) activity and/or thermostability relative to the wild-type enzymes that have incidental AAM activity as a result of cross reactivity with alanine. In addition, the present invention is directed to a polynucleotides that encodes for the AAM polypeptides of the present invention, to nucleic acid sequences comprising the polynucleotides, to expression vectors comprising the polynucleotides operatively linked to a promoter, to host cells transformed to express the AAM polypeptides, and to a method for producing the AAM polypeptides of the present invention.

4. 发明申请

WO2007070726A3 A GENE SYNTHESIS KIT 审中-公开
标题翻译：基因合成试剂盒
公开(公告)号：WO2007070726A3
公开(公告)日：2007-09-27
申请号：PCT/US2006048550
申请日：2006-12-18
申请人： GENE ORACLE INC , MITCHELL KENNETH W , COX ANTHONY R
发明人： MITCHELL KENNETH W , COX ANTHONY R
IPC分类号： C12N15/10 , C12N15/11 , G06F19/22
CPC分类号： C12N15/10 , C12N15/1089 , G06F19/20 , G06F19/22
摘要： This disclosure is directed to the field of polynucleotide synthesis, and the embodiments taught herein are generally directed to a kit for use in the design of a desired polynucleotide from information obtained from a polypeptide or another polynucleotide, the generation of a custom set of oligonucleotides that complement the design, and the ordering of the custom set of oligonucleotides. The invention includes systems and methods for producing a desired polynucleotide using the kit.
摘要翻译：本公开涉及多核苷酸合成领域，并且本文教导的实施方案通常涉及用于从多肽或另一多核苷酸获得的信息设计所需多核苷酸的试剂盒，产生定制的一组寡核苷酸，补充设计和定制的寡核苷酸组的排序。本发明包括使用该试剂盒生产所需多核苷酸的系统和方法。

6. 发明申请

WO2007047773A2 ALANINE 2,3-AMINOMUTASES AND RELATED POLYNUCLEOTIDES 审中-公开
标题翻译： ALANINE 2,3-AMINOMUTASES AND Related POLYNUCLEOTIDES
公开(公告)号：WO2007047773A2
公开(公告)日：2007-04-26
申请号：PCT/US2006040741
申请日：2006-10-16
申请人： CODEXIS INC , BALATSKAYA SVETLANA , BORUP BIRTHE , CHATTERJEE RANJINI , DHAWAN ISH , MITCHELL KENNETH W , MUNDORFF EMILY , PARTRIDGE LES , TOBIN MATHEW , FOX RICHARD J
发明人： BALATSKAYA SVETLANA , BORUP BIRTHE , CHATTERJEE RANJINI , DHAWAN ISH , MITCHELL KENNETH W , MUNDORFF EMILY , PARTRIDGE LES , TOBIN MATHEW , FOX RICHARD J
IPC分类号： C12N9/88
CPC分类号： C12N9/90 , C12P13/06
摘要： The present disclosure relates generally to polypeptides having improved alanine 2,3- aminomutase (AAM) activity, the polynucleotides encoding the AAM polypeptides, and expression vectors and host cells for expressing the AAM polypeptides.
摘要翻译：本公开一般涉及具有改善的丙氨酸2,3-氨基变位酶（AAM）活性的多肽，编码AAM多肽的多核苷酸，以及用于表达AAM多肽的表达载体和宿主细胞。

7. 发明申请

WO2006047589A2 IMPROVED ALANINE 2,3-AMINOMUTASES AND RELATED POLYNUCLEOTIDES 审中-公开
标题翻译：改进的丙氨酸2,3-氨基丁酸酯和相关的多核苷酸
公开(公告)号：WO2006047589A2
公开(公告)日：2006-05-04
申请号：PCT/US2005038552
申请日：2005-10-25
申请人： CODEXIS INC , CHATTERJEE RANJINI , MITCHELL KENNETH W , LOUIE SUSAN Y , FOX RICHARD J , CHEN MICHELLE
发明人： CHATTERJEE RANJINI , MITCHELL KENNETH W , LOUIE SUSAN Y , FOX RICHARD J , CHEN MICHELLE
IPC分类号： C12N9/90 , C12N15/10
CPC分类号： C12N9/90
摘要： The present invention is directed to polypeptides that have enhanced alanine 2,3-aminomutase (AAM) activity and/or thermostability relative to the wild-type enzymes that have incidental AAM activity as a result of cross reactivity with alanine. In addition, the present invention is directed to a polynucleotides that encodes for the AAM polypeptides of the present invention, to nucleic acid sequences comprising the polynucleotides, to expression vectors comprising the polynucleotides operatively linked to a promoter, to host cells transformed to express the AAM polypeptides, and to a method for producing the AAM polypeptides of the present invention.
摘要翻译：本发明涉及相对于野生型酶具有增强的丙氨酸2,3-氨基变位酶（AAM）活性和/或热稳定性的多肽，所述野生型酶由于与丙氨酸的交叉反应性而具有附带的AAM活性。另外，本发明涉及编码本发明AAM多肽的多核苷酸，包含多核苷酸的核酸序列，包含与启动子可操作地连接的多核苷酸的表达载体，转化以表达AAM的宿主细胞多肽，以及用于生产本发明的AAM多肽的方法。

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