专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

WO1995018220A1 DNA CHAIN USEFUL FOR XANTHOPHYLL SYNTHESIS AND PROCESS FOR PRODUCING XANTHOPHYLLS 审中-公开
标题翻译：用于XANTHOPHYLL合成的DNA链和用于生产XANTHOPHYLLS的方法
公开(公告)号：WO1995018220A1
公开(公告)日：1995-07-06
申请号：PCT/JP1994002220
申请日：1994-12-26
申请人： KIRIN BEER KABUSHIKI KAISHA , MARINE BIOTECHNOLOGY INSTITUTE CO., LTD. , MISAWA, Norihiko , KONDO, Keiji , KAJIWARA, Susumu , YOKOYAMA, Akihiro
发明人： KIRIN BEER KABUSHIKI KAISHA , MARINE BIOTECHNOLOGY INSTITUTE CO., LTD.
IPC分类号： C12N15/00
CPC分类号： C12N9/0093 , C12N9/0004 , C12P23/00
摘要： The following DNA chains relate to xanthophylls having a keto group, represented by astaxanthin, and the following technique relates to a genetically engineered production of xanthophylls: a DNA chain having a base sequence coding for a polypeptide having an enzymatic activity of converting the 4-methylene group of a beta -ionone ring into a keto group; a DNA chain having a base sequence coding for a polypeptide having an enzymatic activity of converting the 4-methylene group of a 3-hydroxy- beta -ionone ring into a keto group; a DNA chain having a base sequence coding for a polypeptide having an enzymatic activity of adding a hydroxyl group to the 3-carbon atom of a 4-keto- beta -ionone ring; and a process for producing various xanthophylls, such as canthaxanthin and astaxanthin, by introducing the above DNA chain(s) into a suitable microorganism, e.g., Escherichia coli , followed by expression thereof.
摘要翻译：以下DNA链涉及由虾青素代表的酮基的叶黄素，以下技术涉及叶黄素的遗传工程改造的生产：具有编码具有转化4-亚甲基的酶活性的多肽的碱基序列的DNA链一组β-噻吩酮环成酮基; 具有编码具有将3-羟基-β-雄酮环的4-亚甲基转化为酮基的酶活性的多肽的碱基序列的DNA链; 具有编码具有向4-酮-β-呋喃酮环的3-碳原子添加羟基的酶活性的多肽的碱基序列的DNA链; 以及通过将上述DNA链引入合适的微生物例如大肠杆菌中而生成各种叶黄素的方法，例如角黄素和虾青素，然后进行表达。

2. 发明申请

WO1996028545A1 DNA STRAND USEFUL IN INCREASING CAROTENOID YIELD 审中-公开
标题翻译： DNA有利于增加卡培他滨的生长
公开(公告)号：WO1996028545A1
公开(公告)日：1996-09-19
申请号：PCT/JP1996000574
申请日：1996-03-08
申请人： KIRIN BEER KABUSHIKI KAISHA , KAJIWARA, Susumu , MISAWA, Norihiko , KONDO, Keiji
发明人： KIRIN BEER KABUSHIKI KAISHA
IPC分类号： C12N15/00
CPC分类号： C12P23/00 , C12N9/90
摘要： A DNA strand having a characteristic of increasing the yield of carotenoid and containing a base sequence which encodes a polypeptide substantially having an amino acid sequence represented by SEQ ID NO: 1 or SEQ ID NO: 2; a DNA strand hybridizable with the above DNA strand; and a process for producing carotenoid which comprises introducing the above-mentioned DNA strand into a carotenoid-producing microorganism and incubating the obtained transformant in a medium to thereby increase the carotenoid content in the culture. This process makes it possible to significantly increase the yield of carotenoid in the microbial biosynthesis of the same.
摘要翻译：具有增加类胡萝卜素产率并含有编码基本上具有由SEQ ID NO：1或SEQ ID NO：2表示的氨基酸序列的多肽的碱基序列的特征的DNA链; 可与上述DNA链杂交的DNA链; 以及生产类胡萝卜素的方法，其包括将上述DNA链引入产生类胡萝卜素的微生物中，并将所得转化体在培养基中培养，从而增加培养物中的类胡萝卜素含量。该方法可以显着提高类胡萝卜素在微生物生物合成中的产量。

3. 发明申请

WO1996006172A1 KETO GROUP INTRODUCING ENZYME, DNA CODING FOR THE SAME, AND PROCESS FOR PRODUCING KETOCAROTENOID 审中-公开
标题翻译： KETO集团介绍ENZYME，DNA编码及其生产方法KETOCAROTENOID
公开(公告)号：WO1996006172A1
公开(公告)日：1996-02-29
申请号：PCT/JP1995001640
申请日：1995-08-18
申请人： KIRIN BEER KABUSHIKI KAISHA , KAJIWARA, Susumu , MISAWA, Norihiko , KONDO, Keiji
发明人： KIRIN BEER KABUSHIKI KAISHA
IPC分类号： C12N15/53
CPC分类号： C12N9/0004 , C12P7/26 , C12P23/00
摘要： A polypeptide having the enzymatic activity of converting the 4-methylene group of a beta -ionone compound into a keto group; a DNA containing the base sequence coding for the above polypeptide; another DNA which hybridizes with the above DNA and contains the base sequence coding for the above polypeptide; still another DNA which has been inserted into plasmid pHP51 and contains the base sequence coding for the above polypeptide; a recombinant vector containing the above DNA(s); a microbe having the above DNA(s) introduced thereinto; and a process for producing a ketocarotenoid which comprises culturing the above microbe in a medium and separating the formed ketocarotenoid from the product of culture. The introduction of the above DNAs as foreign genes into microbes, such as E. coli, followed by expression thereof makes it possible to impart to the microbes the capability of biosynthesis of astaxanthin, 4-ketozeaxanthin, canthaxanthin, echinenone and other ketocarotenoids. The use of such microbes makes it possible to mass-produce ketocarotenoids at reduced cost and labor.
摘要翻译：具有将β-戊酮化合物的4-亚甲基转化为酮基的酶活性的多肽; 含有编码上述多肽的碱基序列的DNA; 与上述DNA杂交并含有编码上述多肽的碱基序列的另一种DNA; 仍然是已经插入质粒pHP51并且含有编码上述多肽的碱基序列的另一个DNA; 含有上述DNA的重组载体; 将上述DNA引入其中的微生物; 以及生产酮类胡萝卜素的方法，其包括在培养基中培养上述微生物，并将所形成的酮类胡萝卜素与培养产物分离。将上述DNA作为外源基因导入微生物，如大肠杆菌，随后进行表达，可以使微生物赋予虾青素，4-酮基黄豆素，角黄素，海参酮等酮类胡萝卜素的生物合成能力。使用这样的微生物使得可以以降低的成本和劳动量大量生产酮类胡萝卜素。

4. 发明申请

WO1995032289A1 TRANSFORMANT LINE OF CANDIDA UTILIS YEAST AND EXPRESSION OF HETEROGENE THEREWITH 审中-公开
标题翻译：坎迪达酵母菌的转化体系及其异构体的表达
公开(公告)号：WO1995032289A1
公开(公告)日：1995-11-30
申请号：PCT/JP1995001005
申请日：1995-05-25
申请人： KIRIN BEER KABUSHIKI KAISHA , KONDO, Keiji , KAJIWARA, Susumu , MISAWA, Norihiko
发明人： KIRIN BEER KABUSHIKI KAISHA
IPC分类号： C12N15/31
CPC分类号： C12N9/14 , C07K14/40 , C12N9/88 , C12N15/815
摘要： A reproducible transformant line of a Candida utilis yeast; a method of expressing a heterogene in the above line; a vector utilizable in the above line and method of expression; and a novel DNA group. The method of expressing a heterogene in a Candida utilis yeast comprises transforming Candida utilis with a vector containing a drug-resistant marker, a sequence homologous with that of a chromosomal (DNA) of a Candida utilis yeast and a heterogene, culturing the resultant transformant, and isolating the product of expression of the heterogene.
摘要翻译：产朊假丝酵母的可重现的转化体系; 在上述行中表达异质的方法; 上述可利用的载体和表达方法; 和一个新的DNA组。在产朊假丝酵母中表达异源的方法包括用含有药物抗性标记的载体转化产朊假丝酵母，与产朊假丝酵母和异源的染色体（DNA）同源的序列，培养所得的转化体，并分离异源基因的表达产物。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式