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1. 发明授权

US5370996A Fatty acyl reductases 失效
标题翻译：脂肪酰基还原酶
公开(公告)号：US5370996A
公开(公告)日：1994-12-06
申请号：US920430
申请日：1992-07-31
申请人： James G. Metz , Michael R. Pollard , Michael W. Lassner
发明人： James G. Metz , Michael R. Pollard , Michael W. Lassner
IPC分类号： C12N9/02 , C12N9/10 , C12N15/54 , C12N15/82 , C12P7/64 , C12P21/00 , C07H21/04 , C12N5/00
CPC分类号： C12N9/0008 , C12N15/8247 , C12N9/1029 , C12Y102/01042
摘要： By this invention, a partially purified seed-plant fatty acyl reductase protein is provided, wherein said protein is active in the formation of a fatty alcohol from a fatty acyl substrate. Of special interest are jojoba embryo reductase proteins having molecular mass of about 54 and 52 kD and sequences obtainable therefrom. Also considered are amino acid and nucleic acid sequences obtainable from such fatty acyl reductases, which sequences may be used for preparation of recombinant constructs useful for expression of reductase in host cells, which results in the production of fatty alcohols in said cells.
摘要翻译：通过本发明，提供了部分纯化的种子脂肪酰基还原酶蛋白质，其中所述蛋白质从脂肪酰基底物形成脂肪醇是有活性的。特别感兴趣的是具有约54和52kD的分子量的霍霍巴胚胎还原酶蛋白和可从其获得的序列。还考虑了可从这样的脂肪酰基还原酶获得的氨基酸和核酸序列，该序列可用于制备用于在宿主细胞中表达还原酶的重组构建体，其导致在所述细胞中产生脂肪醇。

2. 发明授权

US07932433B2 Plant cyclopropane fatty acid synthase genes, proteins, and uses thereof 有权
标题翻译：植物环丙烷脂肪酸合成酶基因，蛋白质及其用途
公开(公告)号：US07932433B2
公开(公告)日：2011-04-26
申请号：US12246731
申请日：2008-10-07
申请人： Xiaoming Bao , John B. Ohlrogge , Michael R. Pollard
发明人： Xiaoming Bao , John B. Ohlrogge , Michael R. Pollard
IPC分类号： C12N15/82 , C07H21/04
CPC分类号： C12Q1/6888 , A01K2227/40 , C07K14/705 , C12Q2600/124 , C12Q2600/156
摘要： Cyclopropane fatty acid synthase genes and polypeptides are described. Plants are transformed with such genes to produce such polypeptides.
摘要翻译：描述了环丙烷脂肪酸合成酶基因和多肽。用这些基因转化植物以产生这样的多肽。

3. 发明授权

US07122367B2 Diacylglycerol acyltransferase genes, proteins, and uses thereof 失效
标题翻译：二酰基甘油酰基转移酶基因，蛋白质及其用途
公开(公告)号：US07122367B2
公开(公告)日：2006-10-17
申请号：US10859247
申请日：2004-06-02
申请人： Anne Milcamps , David A. Pan , Michael R. Pollard
发明人： Anne Milcamps , David A. Pan , Michael R. Pollard
IPC分类号： C12N1/20 , C12N9/12 , C12N1/00 , C12N5/08 , C07H21/02 , C07H21/04
CPC分类号： C12N9/1029 , C12N15/8247
摘要： The present invention relates to diacylglycerol acyltransferase genes and proteins, and methods of their use. In particular, the invention describes genes and proteins that exhibit both long-chain acyltransferase and acetyltransferase activity. The present invention encompasses both native and recombinant wild-type forms of the transferase, as well as mutants and variant forms, some of which possess altered characteristics relative to the wild-type transferase. The present invention also relates to methods of using diacylglycerol acyltransferase genes and proteins, including in their expression in transgenic organisms and in the production of acetyl-glycerides in plant oils, and in particular seed oils.
摘要翻译：本发明涉及二酰基甘油酰基转移酶基因和蛋白质及其使用方法。特别地，本发明描述了展现长链酰基转移酶和乙酰转移酶活性的基因和蛋白质。本发明包括天然和重组野生型形式的转移酶，以及突变体和变体形式，其中一些相对于野生型转移酶具有改变的特征。本发明还涉及使用二酰基甘油酰基转移酶基因和蛋白质的方法，包括在转基因生物体中的表达和在植物油中特别是种子油中生产乙酰甘油酯。

4. 发明授权

US6127180A Pesticidal toxins 失效
公开(公告)号：US6127180A
公开(公告)日：2000-10-03
申请号：US844188
申请日：1997-04-18
申请人： Kenneth E. Narva , H. Ernest Schnepf , Mark Knuth , Michael R. Pollard , Guy Cardineau , George E. Schwab , Tracy Ellis Michaels
发明人： Kenneth E. Narva , H. Ernest Schnepf , Mark Knuth , Michael R. Pollard , Guy Cardineau , George E. Schwab , Tracy Ellis Michaels
IPC分类号： C12N15/09 , A01N37/18 , A01N63/00 , A01N63/02 , C07K14/325 , C12N1/20 , C12N1/21 , C12N5/04 , C12N5/10 , C12N15/00 , C12N15/32 , C12N15/82 , C12Q1/68 , C12R1/07
CPC分类号： C07K14/325 , C12N2799/026
摘要： The subject invention concerns new classes of pesticidal toxins and the polynucleotide sequences which encode these toxins. Also described are novel pesticidal isolates of Bacillus thuringiensis.

5. 发明授权

US07446188B2 Plant cyclopropane fatty acid synthase genes, proteins, and uses thereof 失效
标题翻译：植物环丙烷脂肪酸合成酶基因，蛋白质及其用途
公开(公告)号：US07446188B2
公开(公告)日：2008-11-04
申请号：US10499246
申请日：2002-12-20
申请人： Xiaoming Bao , John B. Ohlrogge , Michael R. Pollard
发明人： Xiaoming Bao , John B. Ohlrogge , Michael R. Pollard
IPC分类号： C07H21/04 , A61K36/00
CPC分类号： C12N9/1007 , C12N15/8247
摘要： The present invention provides cyclopropane fatty acid synthase genes and proteins, and methods of their use. The present invention encompasses both native and recombinant wild-type forms of the synthase, as well as mutants and variant forms, some of which possess altered characteristics relative to the wild-type synthase. The present invention also provides methods of using cyclopropane fatty acid synthase genes and proteins, including in their expression in transgenic organisms and in the production of cyclopropane fatty acids in plant oils, and in particular seed oils.
摘要翻译：本发明提供了环丙烷脂肪酸合酶基因和蛋白质及其使用方法。本发明包括合成酶的天然和重组野生型形式，以及突变体和变体形式，其中一些相对于野生型合酶具有改变的特征。本发明还提供了使用环丙烷脂肪酸合酶基因和蛋白质的方法，包括在转基因生物体中的表达和在植物油中特别是种子油中的环丙烷脂肪酸的生产。

6. 发明授权

US07429473B2 Diacylglycerol acyltransferase genes, proteins, and uses thereof 有权
标题翻译：二酰基甘油酰基转移酶基因，蛋白质及其用途
公开(公告)号：US07429473B2
公开(公告)日：2008-09-30
申请号：US11541881
申请日：2006-10-02
申请人： Anne Milcamps , David A. Pan , Michael R. Pollard
发明人： Anne Milcamps , David A. Pan , Michael R. Pollard
IPC分类号： C12N9/10 , C12N15/82 , C07H21/04
CPC分类号： C12N9/1029 , C12N15/8247
摘要： The present invention relates to diacylglycerol acyltransferase genes and proteins, and methods of their use. In particular, the invention describes genes and proteins that exhibit both long-chain acyltransferase and acetyltransferase activity. The present invention encompasses both native and recombinant wild-type forms of the transferase, as well as mutants and variant forms, some of which possess altered characteristics relative to the wild-type transferase. The present invention also relates to methods of using diacylglycerol acyltransferase genes and proteins, including in their expression in transgenic organisms and in the production of acetyl-glycerides in plant oils, and in particular seed oils.
摘要翻译：本发明涉及二酰基甘油酰基转移酶基因和蛋白质及其使用方法。特别地，本发明描述了展现长链酰基转移酶和乙酰转移酶活性的基因和蛋白质。本发明包括天然和重组野生型形式的转移酶，以及突变体和变体形式，其中一些相对于野生型转移酶具有改变的特征。本发明还涉及使用二酰基甘油酰基转移酶基因和蛋白质的方法，包括在转基因生物体中的表达和在植物油中特别是种子油中生产乙酰甘油酯。

7. 发明授权

US07355003B2 Pesticidal proteins 有权
标题翻译：杀虫蛋白
公开(公告)号：US07355003B2
公开(公告)日：2008-04-08
申请号：US11037780
申请日：2005-01-18
申请人： Kenneth E. Narva , H. Ernest Schnepf , Mark Knuth , Michael R. Pollard , Guy A. Cardineau , George E. Schwab , Tracy Ellis Michaels
发明人： Kenneth E. Narva , H. Ernest Schnepf , Mark Knuth , Michael R. Pollard , Guy A. Cardineau , George E. Schwab , Tracy Ellis Michaels
IPC分类号： C07K14/325 , A01N63/02 , C12N15/32
CPC分类号： C12R1/075 , C07K14/325
摘要： The subject invention concerns new classes of pesticidally active proteins and the polynucleotide sequences which encode these proteins. More specifically, in preferred embodiments, pesticidal proteins of approximately 40-50 kDa and of approximately 10-15 kDa are used for controlling corn rootworms. Also described are novel pesticidal isolates of Bacillus thuringiensis.
摘要翻译：本发明涉及新类型的杀虫活性蛋白质和编码这些蛋白质的多核苷酸序列。更具体地，在优选的实施方案中，使用约40-50kDa和约10-15kDa的杀虫蛋白来控制玉米根虫。还描述了苏云金芽孢杆菌的新型杀虫分离物。

8. 发明授权

US06900371B2 Pesticidal proteins 失效
公开(公告)号：US06900371B2
公开(公告)日：2005-05-31
申请号：US10099278
申请日：2002-03-15
申请人： Kenneth E. Narva , H. Ernest Schnepf , Mark Knuth , Michael R. Pollard , Guy A. Cardineau , George E. Schwab , Tracy Ellis Michaels , Stacey Finstad Lee , Paula Burmeister , Joanna Dojillo
发明人： Kenneth E. Narva , H. Ernest Schnepf , Mark Knuth , Michael R. Pollard , Guy A. Cardineau , George E. Schwab , Tracy Ellis Michaels , Stacey Finstad Lee , Paula Burmeister , Joanna Dojillo
IPC分类号： C12N15/09 , A01H1/00 , A01H5/00 , A01H5/10 , A01N37/18 , A01N63/00 , A01N63/02 , C07H21/04 , C07K14/325 , C12N1/15 , C12N1/19 , C12N1/20 , C12N1/21 , C12N5/04 , C12N5/10 , C12N9/00 , C12N15/32 , C12N15/62 , C12N15/82 , C12P21/02 , C12R1/07 , C12R1/39
CPC分类号： C12R1/075 , C07K14/325
摘要： The subject invention concerns new classes of pesticidally active proteins and the polynucleotide sequences which encode these proteins. More specifically, in preferred embodiments, pesticidal proteins of approximately 40-50 kDa and of approximately 10-15 kDa are used for controlling corn rootworms. Also described are novel pesticidal isolates of Bacillus thuringiensis.

9. 发明授权

US06372480B1 Pesticidal proteins 有权
标题翻译：杀虫蛋白
公开(公告)号：US06372480B1
公开(公告)日：2002-04-16
申请号：US09378088
申请日：1999-08-20
申请人： Kenneth E. Narva , H. Ernest Schnepf , Mark Knuth , Michael R. Pollard , Guy A. Cardineau , George E. Schwab , Tracy Ellis Michaels , Stacey Finstad Lee , Paula Burmeister , Joanna Dojillo
发明人： Kenneth E. Narva , H. Ernest Schnepf , Mark Knuth , Michael R. Pollard , Guy A. Cardineau , George E. Schwab , Tracy Ellis Michaels , Stacey Finstad Lee , Paula Burmeister , Joanna Dojillo
IPC分类号： C07K14325
CPC分类号： C12R1/075 , C07K14/325
摘要： The subject invention concerns new classes of pesticidally active proteins and the polynucleotide sequences which encode these proteins. More specifically, in preferred embodiments, pesticidal proteins of approximately 40-50 kDa and of approximately 10-15 kDa are used for controlling corn rootworms. Also described are novel pesticidal isolates of Bacillus thuringiensis.
摘要翻译：本发明涉及新类型的杀虫活性蛋白质和编码这些蛋白质的多核苷酸序列。更具体地，在优选的实施方案中，使用约40-50kDa和约10-15kDa的杀虫蛋白来控制玉米根虫。还描述了苏云金芽孢杆菌的新型杀虫分离物。

10. 发明授权

US5603978A Esterified propylene oxide-extended glycerols useful as fat substitutes 失效
标题翻译：用作脂肪替代物的酯化环氧丙烷扩展的甘油
公开(公告)号：US5603978A
公开(公告)日：1997-02-18
申请号：US476815
申请日：1995-06-07
申请人： John F. White , Michael R. Pollard
发明人： John F. White , Michael R. Pollard
IPC分类号： A23L1/307 , A23L1/30 , A23L1/308 , C07D303/24 , A23D3/00 , A23D5/00
CPC分类号： A23L33/26 , A23L33/115 , A23L33/25 , Y10S426/804
摘要： Low calorie food compositions containing fat-type organoleptic ingredients and non-fat ingredients, wherein natural fats and synthetic fat mimetics are blended in proportion to provide any predetermined amount of fat caloric value, said synthetic fat-type mimetic ingredients comprising esterified epoxide-extended polyols (EEEPs) of the formula P(OH).sub.a+c (EPO).sub.n (FE).sub.b where P is a polyol having a=2-8 primary hydroxyls, c=0-8 secondary and tertiary hydroxyls, a+c is in the range of 3-8, EPO is a C.sub.3 -C.sub.6 epoxide, FE is a fatty acid acyl moiety, n is the minimum epoxylation index average number having a value generally equal to or greater than a and is a number sufficient that greater than 95% of the primary hydroxyls of the polyol are converted to secondary or tertiary hydroxyls, and 2
摘要翻译：含有脂肪型感官成分和非脂肪成分的低热量食物组合物，其中天然脂肪和合成脂肪模拟物按比例混合以提供任何预定量的脂肪热值，所述合成脂肪型模拟成分包含酯化的环氧化物延长的多元醇（EPO）n（FE）b的式（EEEP），其中P是具有= 2-8个伯羟基的多元醇，c = 0-8仲羟基和叔羟基，a + c在 3-8的范围，EPO是C3-C6环氧化物，FE是脂肪酸酰基部分，n是具有通常等于或大于a的值的最小环氧化指数平均数，并且是足以大于95的数多元醇的伯羟基的％转化为仲羟基或叔羟基，并且2

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