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1. 发明申请

WO2004096978A2 PRODUCTION OF HIGH MANNOSE PROTEINS IN PLANT CULTURE 审中-公开
标题翻译：在植物文化中生产高含量的蛋白质
公开(公告)号：WO2004096978A2
公开(公告)日：2004-11-11
申请号：PCT/IL2004/000181
申请日：2004-02-24
申请人： METABOGAL LTD. , SHAALTIEL, Yoseph , BAUM, Gideon , BARTFELD, Daniel
发明人： SHAALTIEL, Yoseph , BAUM, Gideon , BARTFELD, Daniel
IPC分类号： C12N
CPC分类号： C12P21/005 , C07K2319/04 , C12N9/2402 , C12N15/8257 , C12Y302/01045
摘要： A device, system and method for producing glycosylated proteins in plant culture, particularly proteins having a high mannose glycosylation, while targeting such proteins with an ER signal and/or by-passing the Golgi. The invention further relates to vectors and methods for expression and production of enzymatically active high mannose lysosomal enzymes using transgenic plant root, particularly carrot cells. More particularly, the invention relates to host cells, particularly transgenic suspended carrot cells, vectors and methods for high yield expression and production of biologically active high mannose Glucocerebrosidase (GCD). The invention further provides for compositions and methods for the treatment of lysosomal storage diseases.
摘要翻译：用于在植物培养物中生产糖基化蛋白质的装置，系统和方法，特别是具有高甘露糖糖基化的蛋白质，同时用ER信号靶向这种蛋白质和/或旁路高尔基体。本发明进一步涉及使用转基因植物根特别是胡萝卜细胞表达和产生酶活性高甘露糖溶酶体酶的载体和方法。更具体地，本发明涉及宿主细胞，特别是转基因悬浮的胡萝卜细胞，用于高产量表达和生产生物活性高甘露糖葡糖脑苷脂酶（GCD）的载体和方法。本发明还提供了用于治疗溶酶体贮积病的组合物和方法。

2. 发明申请

WO1998013469A1 CELL/TISSUE CULTURING DEVICE AND METHOD 审中-公开
标题翻译：细胞/组织培养装置和方法
公开(公告)号：WO1998013469A1
公开(公告)日：1998-04-02
申请号：PCT/IL1997000316
申请日：1997-09-26
申请人： METABOGAL LTD. , SHAALTIEL, Yoseph
发明人： METABOGAL LTD.
IPC分类号： C12M01/04
CPC分类号： C12M23/14 , C12M23/22 , C12M23/28 , C12M29/00 , C12M33/00 , C12M41/44
摘要： A disposable device and method for axenically culturing and harvesting cells and/or tissue in consecutive cycles. The device consists of a sterilisable transparent and/or translucent disposable container which may be at least partially filled with a suitable sterile biological cell and/or tissue culture medium and/or axenic inoculant and/or sterile air and/or required other sterile additives. The container has means for removing excess air and/or waste gases therefrom, and means for introducing the inoculant and/or culture medium and/or additives therein. The device is characterised by having a reusable harvesting means for enabling harvesting of at least a portion of the medium containing cells and/or tissue when desired, thereby enabling the device to be used continuously for at least one subsequent consecutive culturing/harvesting cycle. The portion of medium containing cells and/or tissue remaining from a previously harvested cycle may serve as inoculant for a next culture and harvest cycle, culture medium and/or additives being provided. The device may thus be used continuously in consecutive cycles, and may be disposed of when it becomes contaminated. In a second aspect of the invention, a battery of these devices, suitably interconnected, enables the scale of production of cells/tissues to be adjusted when required.
摘要翻译：一种用于在连续循环中进行细胞和/或组织的细胞培养和收获的一次性装置和方法。该装置由可灭菌的透明和/或半透明的一次性容器组成，其可以至少部分地填充有合适的无菌生物细胞和/或组织培养基和/或无菌孕育剂和/或无菌空气和/或所需的其它无菌添加剂。容器具有从其中除去多余的空气和/或废气的装置，以及用于将孕育剂和/或培养基和/或添加剂引入其中的装置。该装置的特征在于具有可重复使用的收获装置，用于在需要时能够收集含有细胞和/或组织的至少一部分培养基，从而使得该装置能够连续使用至少一个随后的连续培养/收获循环。从先前收获的循环中残留的含有细胞和/或组织的培养基的一部分可以用作下一培养和收获循环的接种剂，提供培养基和/或添加剂。因此，该装置可以在连续循环中连续使用，并且可以在其被污染时被处理。在本发明的第二方面，这些装置的电池适当互连使得能够在需要时调节细胞/组织的生产规模。

3. 发明申请

US20020110915A1 Cell/tissue culturing device and method 审中-公开
标题翻译：细胞/组织培养装置及方法
公开(公告)号：US20020110915A1
公开(公告)日：2002-08-15
申请号：US10121534
申请日：2002-04-12
申请人： Metabogal Ltd.
发明人： Yoseph Shaaltiel
IPC分类号： C12M001/04 , C12M001/24 , C12N005/02
CPC分类号： C12M23/14 , C12M23/22 , C12M23/28 , C12M29/00 , C12M33/00 , C12M41/44
摘要： A disposable device and method for axenically culturing and harvesting cells and/or tissue in consecutive cycles. The device consist of a sterilizable transparent and/or translucent disposable container which may be at least partially filled with a suitable sterile biological cell and/or tissue culture medium and/or axenic inoculant and/or sterile air and/or required other sterile additives. The container has means for removing excess air and/or waste gases therefrom, and means for introducing the inoculant and/or culture medium and/or additives therein. The device also has at least one air inlet, and preferably a plurality thereof, for introducing sterile air into the device. The device is characterised by having a reusable harvesting means comprising suitable flow control means for enabling harvesting of at least a portion of the medium containing cells and/or tissue when desired, thereby enabling the device to be used continuously for at least one subsequent consecutive culturing/harvesting cycle. The portion of medium containing cells and/or tissue remaining from a previously harvested cycle may serve as inoculant for a next culture and harvest cycle, culture medium and/or additives being provided. The device may thus be used continuously in consecutive cycles, and may be disposed of when it becomes contaminated. In a second aspect of the invention, a battery of these devices, suitably interconnected, enables the scale of production of cells/tissues to be adjusted when required.
摘要翻译：一种用于在连续循环中进行细胞和/或组织的细胞培养和收获的一次性装置和方法。该装置由可灭菌的透明和/或半透明的一次性容器组成，其可以至少部分地填充有合适的无菌生物细胞和/或组织培养基和/或无菌孕育剂和/或无菌空气和/或所需的其它无菌添加剂。容器具有从其中除去多余的空气和/或废气的装置，以及用于将孕育剂和/或培养基和/或添加剂引入其中的装置。该装置还具有用于将无菌空气引入装置中的至少一个空气入口，并且优选地多个。该装置的特征在于具有可重复使用的收获装置，其包括合适的流动控制装置，用于在需要时能够收集至少一部分含有细胞和/或组织的培养基，从而使得该装置能够连续使用至少一个随后的连续培养 /收获周期。从先前收获的循环中残留的含有细胞和/或组织的培养基的一部分可以用作下一培养和收获循环的接种剂，提供培养基和/或添加剂。因此，该装置可以在连续循环中连续使用，并且可以在其被污染时被处理。在本发明的第二方面，这些装置的电池适当互连使得能够在需要时调节细胞/组织的生产规模。

4. 发明专利

CA2266851C CELL/TISSUE CULTURING DEVICE AND METHOD 未知
公开(公告)号：CA2266851C
公开(公告)日：2009-07-07
申请号：CA2266851
申请日：1997-09-26
申请人： METABOGAL LTD
发明人： SHAALTIEL YOSEPH
IPC分类号： C12M3/02 , C12M1/00 , C12M1/04 , C12M1/12 , C12M1/26
摘要： A disposable device and method for axenically culturing and harvesting cells and/or tissue in consecutive cycles. The device consists of a sterilisable transparent and/or translucent disposable container which may be at least partially filled with a suitable sterile biological cell and/or tissue cultu re medium and/or axenic inoculant and/or sterile air and/or required other sterile additives. The container has means for removing excess air and/or waste gases therefrom , and means for introducing the inoculant and/or culture medium and/or additiv es therein. The device is characterised by having a reusable harvesting means for enabling harvesting of at least a portion of the medium containing cells and/or tissue when desired, thereby enabling the device to be used continuously for at least one subsequent consecutive culturing/harvesting cycle. The portion of medium containing cells and/or tissue remaining from a previously harvest ed cycle may serve as inoculant for a next culture and harvest cycle, culture medium and/or additives being provided. The device may thus be used continuously in consecutive cycles, and may be disposed of when it becomes contaminated. In a second aspect of the invention, a battery of these devices, suitably interconnected, enables the scale of production of cells/tissues to be adjusted when required.

5. 发明专利

PL332483A1 METHOD OF AND APPARATUS FOR CARRYING ON CELL/TISSUE CULTURES 未知
公开(公告)号：PL332483A1
公开(公告)日：1999-09-13
申请号：PL33248397
申请日：1997-09-26
申请人： METABOGAL LTD
发明人： SHAALTIEL YOSEPH
IPC分类号： C12M1/00 , C12M1/04 , C12M1/12 , C12M1/26 , C12M3/02
摘要： A disposable device and method for axenically culturing and harvesting cells and/or tissue in consecutive cycles. The device consist of a sterilizable transparent and/or translucent disposable container which may be at least partially filled with a suitable sterile biological cell and/or tissue culture medium and/or axenic inoculant and/or sterile air and/or required other sterile additives. The container has means for removing excess air and/or waste gases therefrom, and means for introducing the inoculant and/or culture medium and/or additives therein. The device also has at least one air inlet, and preferably a plurality thereof, for introducing sterile air into the device. The device is characterised by having a reusable harvesting means comprising suitable flow control means for enabling harvesting of at least a portion of the medium containing cells and/or tissue when desired, thereby enabling the device to be used continuously for at least one subsequent consecutive culturing/harvesting cycle. The portion of medium containing cells and/or tissue remaining from a previously harvested cycle may serve as inoculant for a next culture and harvest cycle, culture medium and/or additives being provided. The device may thus be used continuously in consecutive cycles, and may be disposed of when it becomes contaminated. In a second aspect of the invention, a battery of these devices, suitably interconnected, enables the scale of production of cells/tissues to be adjusted when required.

6. 发明专利

IL119310A Cell/tissue culturing device and method 未知
公开(公告)号：IL119310A
公开(公告)日：1999-07-14
申请号：IL11931096
申请日：1996-09-26
申请人： METABOGAL LTD
IPC分类号： C12M1/00 , C12M1/04 , C12M1/12 , C12M1/26 , C12M3/02 , G01N33/50
摘要： A disposable device and method for axenically culturing and harvesting cells and/or tissue in consecutive cycles. The device consist of a sterilizable transparent and/or translucent disposable container which may be at least partially filled with a suitable sterile biological cell and/or tissue culture medium and/or axenic inoculant and/or sterile air and/or required other sterile additives. The container has means for removing excess air and/or waste gases therefrom, and means for introducing the inoculant and/or culture medium and/or additives therein. The device also has at least one air inlet, and preferably a plurality thereof, for introducing sterile air into the device. The device is characterised by having a reusable harvesting means comprising suitable flow control means for enabling harvesting of at least a portion of the medium containing cells and/or tissue when desired, thereby enabling the device to be used continuously for at least one subsequent consecutive culturing/harvesting cycle. The portion of medium containing cells and/or tissue remaining from a previously harvested cycle may serve as inoculant for a next culture and harvest cycle, culture medium and/or additives being provided. The device may thus be used continuously in consecutive cycles, and may be disposed of when it becomes contaminated. In a second aspect of the invention, a battery of these devices, suitably interconnected, enables the scale of production of cells/tissues to be adjusted when required.

7. 发明专利

AU4318097A Cell/tissue culturing device and method 未知
公开(公告)号：AU4318097A
公开(公告)日：1998-04-17
申请号：AU4318097
申请日：1997-09-26
申请人： METABOGAL LTD
发明人： SHAALTIEL YOSEPH
IPC分类号： C12M1/00 , C12M1/04 , C12M1/12 , C12M1/26 , C12M3/02
摘要： A disposable device and method for axenically culturing and harvesting cells and/or tissue in consecutive cycles. The device consist of a sterilizable transparent and/or translucent disposable container which may be at least partially filled with a suitable sterile biological cell and/or tissue culture medium and/or axenic inoculant and/or sterile air and/or required other sterile additives. The container has means for removing excess air and/or waste gases therefrom, and means for introducing the inoculant and/or culture medium and/or additives therein. The device also has at least one air inlet, and preferably a plurality thereof, for introducing sterile air into the device. The device is characterised by having a reusable harvesting means comprising suitable flow control means for enabling harvesting of at least a portion of the medium containing cells and/or tissue when desired, thereby enabling the device to be used continuously for at least one subsequent consecutive culturing/harvesting cycle. The portion of medium containing cells and/or tissue remaining from a previously harvested cycle may serve as inoculant for a next culture and harvest cycle, culture medium and/or additives being provided. The device may thus be used continuously in consecutive cycles, and may be disposed of when it becomes contaminated. In a second aspect of the invention, a battery of these devices, suitably interconnected, enables the scale of production of cells/tissues to be adjusted when required.

8. 发明专利

IL155588D0 METHODS FOR EXPRESSION OF ENZYMATICALLY ACTIVE RECOMBINANT LYSOSOMAL ENZYMES IN TRANSGENIC PLANT ROOT CELLS AND VECTORS USED THEREBY 未知
公开(公告)号：IL155588D0
公开(公告)日：2003-11-23
申请号：IL15558803
申请日：2003-04-27
申请人： METABOGAL LTD
IPC分类号： C12M1/00 , C12N20060101 , C12N9/24 , C12N15/82 , C12P21/00 , C12N
摘要： The present invention relates to a pharmaceutical composition comprising, as an active ingredient, plant cells expressing a high mannose recombinant protein and a pharmaceutically acceptable carrier.

9. 发明专利

AT231912T 未知
公开(公告)号：AT231912T
公开(公告)日：2003-02-15
申请号：AT97941180
申请日：1997-09-26
申请人： METABOGAL LTD
发明人： SHAALTIEL YOSEPH
IPC分类号： C12M1/00 , C12M1/04 , C12M1/12 , C12M1/26 , C12M3/02
摘要： A disposable device and method for axenically culturing and harvesting cells and/or tissue in consecutive cycles. The device consist of a sterilizable transparent and/or translucent disposable container which may be at least partially filled with a suitable sterile biological cell and/or tissue culture medium and/or axenic inoculant and/or sterile air and/or required other sterile additives. The container has means for removing excess air and/or waste gases therefrom, and means for introducing the inoculant and/or culture medium and/or additives therein. The device also has at least one air inlet, and preferably a plurality thereof, for introducing sterile air into the device. The device is characterised by having a reusable harvesting means comprising suitable flow control means for enabling harvesting of at least a portion of the medium containing cells and/or tissue when desired, thereby enabling the device to be used continuously for at least one subsequent consecutive culturing/harvesting cycle. The portion of medium containing cells and/or tissue remaining from a previously harvested cycle may serve as inoculant for a next culture and harvest cycle, culture medium and/or additives being provided. The device may thus be used continuously in consecutive cycles, and may be disposed of when it becomes contaminated. In a second aspect of the invention, a battery of these devices, suitably interconnected, enables the scale of production of cells/tissues to be adjusted when required.

10. 发明专利

CZ9901047A3 CULTIVATION PROCESS OF CELLS AND TISSUES AS WELL AS APPARATUS FOR MAKING THE SAME 未知
公开(公告)号：CZ9901047A3
公开(公告)日：1999-08-11
申请号：CZ104799
申请日：1997-09-26
申请人： METABOGAL LTD
发明人： SHAALTIEL YOSEPH
IPC分类号： C12M1/00 , C12M1/04 , C12M1/12 , C12M1/26 , C12M3/02
CPC分类号： C12M23/14 , C12M23/22 , C12M23/28 , C12M29/00 , C12M33/00 , C12M41/44

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