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1. 发明申请

WO0187914A3 PLANT GENE TARGETING USING OLIGONUCLEOTIDES 审中-公开
标题翻译：使用寡核苷酸的植物基因定位
公开(公告)号：WO0187914A3
公开(公告)日：2002-06-13
申请号：PCT/US0116152
申请日：2001-05-17
申请人： UNIV DELAWARE , MAY GREGORY D , KMIEC ERIC B , RICE MICHAEL C
发明人： MAY GREGORY D , KMIEC ERIC B , RICE MICHAEL C
IPC分类号： C12N15/82 , C07H21/00 , C12N15/10 , C12N15/11
CPC分类号： C12N15/8216 , C12N15/8201 , C12N15/8213 , C12N15/8241
摘要： Methods and compositions are presented for the generation of targeted alterations in a plant genome using double-stranded homogeneous oligonucleotides containing a single type of nucleotide. These methods can be used to correct mutations, introduce mutations and/or alter gene activity in a plant cell. A cell-free assay system for monitoring genetic alteration using the oligonucleotides of the invention is also presented.
摘要翻译：呈现了使用含有单一类型核苷酸的双链均质寡核苷酸在植物基因组中产生靶向改变的方法和组合物。这些方法可用于校正突变，在植物细胞中引入突变和/或改变基因活性。还提供了使用本发明的寡核苷酸监测遗传改变的无细胞测定系统。

2. 发明申请

WO2005033309A2 PLANT PROMOTERS AND METHODS OF USE THEREOF 审中-公开
标题翻译：植物促进剂及其使用方法
公开(公告)号：WO2005033309A2
公开(公告)日：2005-04-14
申请号：PCT/US2004/032365
申请日：2004-09-30
申请人： THE SAMUEL ROBERTS NOBLE FOUNDATION, INC. , DIXON, Richard, A. , WANG, Liangjiang , MAY, Gregory, D.
发明人： DIXON, Richard, A. , WANG, Liangjiang , MAY, Gregory, D.
IPC分类号： C12N15/10
CPC分类号： C07K14/415 , C12N15/8216
摘要： The current invention provides plant promoter sequences. Compositions comprising these promoter sequence are described, as are plants transformed with such compositions. Further provided are methods for the expression of transgenes in plants comprising the use of these sequences. The methods of the invention include the direct creation of transgenic plants with the promoters by genetic transformation, as well as by plant breeding methods. The sequences of the invention represent valuable new tools for the creation of transgenic plants, preferably having one or more added beneficial characteristics.
摘要翻译：本发明提供植物启动子序列。描述了包含这些启动子序列的组合物，以及用这种组合物转化的植物。还提供了在植物中表达转基因的方法，其中包括使用这些序列。本发明的方法包括通过遗传转化直接产生具有启动子的转基因植物，以及通过植物育种方法。本发明的序列代表了用于产生转基因植物的有价值的新工具，优选具有一个或多个添加的有益特征。

3. 发明申请

WO2002059380A2 CELL-FREE ASSAY AND IN VIVO METHOD FOR PLANT GENETIC REPAIR USING CHLOROPLAST LYSATE 审中-公开
标题翻译：无细胞免疫测定法和植物遗传修复方法使用氯化石蜡
公开(公告)号：WO2002059380A2
公开(公告)日：2002-08-01
申请号：PCT/US2002/000338
申请日：2002-01-07
申请人： THE SAMUEL ROBERTS NOBLE FOUNDATION, INC. , MAY, Gregory, D. , KMIEC, Eric, B.
发明人： MAY, Gregory, D. , KMIEC, Eric, B.
IPC分类号： C12Q1/68
CPC分类号： C12N15/102
摘要： An in vivo or in vitro cell-free method for genetic repair of mutations in plastid genes has been found which consists of (1) reacting a plasmid which contains a specific mutation (point mutation or frameshift mutation) of interest, a chimeric RNA/DNA oligonucleotide or a modified single stranded oligonucleotide which is believed to contain the genetic code for correcting the plastid gene mutation, and a chloroplast extract taken from the plant of interest; and (2) determining the success of gene conversion using a genetic readout system. A cell-free assay is disclosed by which the enzymatic capacity of chloroplast extracts to direct gene repair such as corrections to both point mutations and frameshift mutations can be determined. This assay method also enables the mechanistic study of plastid gene repair and facilitates the direct comparison between plant nuclear and organelle DNA repair pathways.
摘要翻译：已经发现体内或体外无细胞遗传修复质体基因突变的方法，其由（1）使包含特定突变（点突变或移码突变）的质粒，嵌合RNA / DNA 寡核苷酸或被认为含有用于校正质体基因突变的遗传密码的修饰单链寡核苷酸，以及从感兴趣的植物获取的叶绿体提取物; 和（2）使用遗传读出系统确定基因转化的成功。公开了无细胞测定法，通过该测定可以确定叶绿体提取物的指导基因修复的酶能力，例如对两个突变和移码突变的校正。该测定方法还能够进行质体基因修复的机械研究，并促进植物核和细胞器质DNA修复途径之间的直接比较。

4. 发明申请

WO2003053135A1 PLANT XRCC3 GENES AND METHODS OF USE 审中-公开
标题翻译：植物XRCC3基因和使用方法
公开(公告)号：WO2003053135A1
公开(公告)日：2003-07-03
申请号：PCT/US2002/040790
申请日：2002-12-20
申请人： BOYCE THOMPSON INSTITUTE FOR PLANT RESEARCH , MAY, Gregory, D. , BEETHAM, Peter, R.
发明人： MAY, Gregory, D. , BEETHAM, Peter, R.
IPC分类号： A01H5/00
CPC分类号： C07K14/415 , C12N15/8213
摘要： The invention relates to isolated nucleic acid molecules encoding plant XRCC3 proteins. Such XRCC3 proteins are believed to be involved in homologous recombination and DNA-repair processes in organisms, particularly plants. The invention provides isolated nucleic acid molecules comprising XRCC3 nucleotide sequences which encode XRCC3 proteins and XRCC3 nucleotide sequences which encode dominant-negative XRCC3 variants. Such XRCC3 nucleotide sequences find use in altering DNA repair, mutation rates and recombination frequencies in both eukaryotic and prokaryotic orgnasims. Additionally provided are isolated proteins, transformed non-human host cells, and transformed plants, tissues, cells and seeds thereof.
摘要翻译：本发明涉及编码植物XRCC3蛋白质的分离的核酸分子。据信这种XRCC3蛋白参与生物体，特别是植物中的同源重组和DNA修复过程。本发明提供了分离的核酸分子，其包含编码XRCC3蛋白的XRCC3核苷酸序列和编码显性阴性XRCC3变体的XRCC3核苷酸序列。这样的XRCC3核苷酸序列可用于改变真核和原核生物中的DNA修复，突变率和重组频率。另外提供了分离的蛋白质，转化的非人宿主细胞和转化的植物，组织，细胞和种子。

5. 发明申请

WO03054217A2 PLANT MSH2 SEQUENCES AND METHODS OF USE 审中-公开
标题翻译：植物MSH2序列及其使用方法
公开(公告)号：WO03054217A2
公开(公告)日：2003-07-03
申请号：PCT/US0240887
申请日：2002-12-20
申请人： THOMPSON BOYCE PLANT RES , PIONEER HI BRED INT , KIPP PETER B , MAY GREGORY D , MAHAJAN PRADMOD B , BASZCZYNSKI CHRISTOPHER L , ZHU TONG
发明人： KIPP PETER B , MAY GREGORY D , MAHAJAN PRADMOD B , BASZCZYNSKI CHRISTOPHER L , ZHU TONG
IPC分类号： C12N9/90 , C12N15/29 , C12N15/82 , C12Q
CPC分类号： C12N9/90 , C12N15/8213 , C12N15/8222
摘要： The invention relates to isolated nucleic acid molecules encoding MutS homologues (MSHs). Such MSH proteins are involved in DNA mismatch-repair processes in organisms. The invention provides isolated nucleic acid molecules comprising MSH2 nucleotide sequences which encode MSH2 proteins and MSH2 nucloetide sequences which encode dominant-negative MSH2 variants. Such MSH2 nucleotide sequences find use in altering mismatch repair, mutation rates and recombination frequencies in both eukaryotic and prokaryotic organisms. The invention also provides isolated nucleic acid molecules comprising MSH2 promoter nucleotide sequences. Such MSH2 promoter nucleotide sequences find use in regulating the expression of genes of interest in plants. Additionally provided are isolated proteins, transformed host cells, and transformed plants, tissues, cells and seeds thereof.
摘要翻译：本发明涉及编码MutS同系物（MSH）的分离的核酸分子。这样的MSH蛋白参与生物体中的DNA错配修复过程。本发明提供分离的核酸分子，其包含编码MSH2蛋白质的MSH2核苷酸序列和编码显性阴性MSH2变体的MSH2核苷酸序列。这样的MSH2核苷酸序列可用于改变真核和原核生物体中的错配修复，突变率和重组频率。本发明还提供了包含MSH2启动子核苷酸序列的分离的核酸分子。这样的MSH2启动子核苷酸序列可用于调节植物中感兴趣的基因的表达。另外提供了分离的蛋白质，转化的宿主细胞和转化的植物，组织，细胞和种子。

6. 发明申请

WO1995015678A1 AGROBACTERIUM TUMEFACIENS TRANSFORMATION OF MUSA SPECIES 审中-公开
标题翻译：农杆菌转化的MUSA物种
公开(公告)号：WO1995015678A1
公开(公告)日：1995-06-15
申请号：PCT/US1994014210
申请日：1994-12-09
申请人： THE TEXAS A & M UNIVERSITY SYSTEM , ARNTZEN, Charles, J. , MAY, Gregory, D.
发明人： THE TEXAS A & M UNIVERSITY SYSTEM
IPC分类号： A01H05/00
CPC分类号： C12N15/8205 , C12N15/8242
摘要： Methods are provided for transforming Musa plants. In particular, methods for wounding meristematic Musa plant tissue to facilitate access of Agrobacterium tumefaciens comprising genetically-engineered T-DNA is provided. The methods may be used to transform the plant to produce pharmaceutical products or to alter the phenotypic trait of the fruit of the plant.
摘要翻译：提供了用于转化Musa植物的方法。特别地，提供了用于伤害分生孢子植物组织以促进包括遗传工程化T-DNA的根癌农杆菌接种的方法。该方法可用于转化植物以产生药物产品或改变植物果实的表型性状。

7. 发明申请

WO2005033309A3 PLANT PROMOTERS AND METHODS OF USE THEREOF 审中-公开
标题翻译：植物启动子及其使用方法
公开(公告)号：WO2005033309A3
公开(公告)日：2005-07-28
申请号：PCT/US2004032365
申请日：2004-09-30
申请人： SAMUEL ROBERTS NOBLE FOUND INC , DIXON RICHARD A , WANG LIANGJIANG , MAY GREGORY D
发明人： DIXON RICHARD A , WANG LIANGJIANG , MAY GREGORY D
IPC分类号： A01H1/00 , C07K14/415 , C12N15/82 , C12Q1/68 , C12N15/29
CPC分类号： C07K14/415 , C12N15/8216
摘要： The current invention provides plant promoter sequences. Compositions comprising these promoter sequence are described, as are plants transformed with such compositions. Further provided are methods for the expression of transgenes in plants comprising the use of these sequences. The methods of the invention include the direct creation of transgenic plants with the promoters by genetic transformation, as well as by plant breeding methods. The sequences of the invention represent valuable new tools for the creation of transgenic plants, preferably having one or more added beneficial characteristics.
摘要翻译：本发明提供了植物启动子序列。描述了包含这些启动子序列的组合物，以及用这些组合物转化的植物。还提供了用于在植物中表达转基因的方法，包括使用这些序列。本发明的方法包括通过遗传转化以及通过植物育种方法直接产生具有启动子的转基因植物。本发明的序列代表了用于产生转基因植物的有价值的新工具，优选具有一种或多种附加的有益特征。

8. 发明申请

WO03053135A9 PLANT XRCC3 GENES AND METHODS OF USE 审中-公开
标题翻译：植物XRCC3基因和使用方法
公开(公告)号：WO03053135A9
公开(公告)日：2004-05-21
申请号：PCT/US0240790
申请日：2002-12-20
申请人： THOMPSON BOYCE PLANT RES , MAY GREGORY D , BEETHAM PETER R
发明人： MAY GREGORY D , BEETHAM PETER R
IPC分类号： C07K14/415 , C12N1/15 , C12N1/21 , C12N15/29 , C12N15/82 , A01H5/00 , A01H5/10 , C12N5/04 , C12N5/06 , C12N15/81 , C12N15/85
CPC分类号： C07K14/415 , C12N15/8213
摘要： The invention relates to isolated nucleic acid molecules encoding plant XRCC3 proteins. Such XRCC3 proteins are believed to be involved in homologous recombination and DNA-repair processes in organisms, particularly plants. The invention provides isolated nucleic acid molecules comprising XRCC3 nucleotide sequences which encode XRCC3 proteins and XRCC3 nucleotide sequences which encode dominant-negative XRCC3 variants. Such XRCC3 nucleotide sequences find use in altering DNA repair, mutation rates and recombination frequencies in both eukaryotic and prokaryotic orgnasims. Additionally provided are isolated proteins, transformed non-human host cells, and transformed plants, tissues, cells and seeds thereof.
摘要翻译：本发明涉及编码植物XRCC3蛋白质的分离的核酸分子。据信这种XRCC3蛋白参与生物体，特别是植物中的同源重组和DNA修复过程。本发明提供了分离的核酸分子，其包含编码XRCC3蛋白的XRCC3核苷酸序列和编码显性阴性XRCC3变体的XRCC3核苷酸序列。这样的XRCC3核苷酸序列可用于改变真核和原核生物中的DNA修复，突变率和重组频率。另外提供了分离的蛋白质，转化的非人宿主细胞和转化的植物，组织，细胞和种子。

9. 发明申请

WO02053779A3 CELL-FREE ASSAY AND IN VIVO METHOD FOR PLANT GENETIC REPAIR USING CHLOROPLAST LYSATE 审中-公开
标题翻译：无细胞免疫测定法和植物遗传修复方法使用氯化石蜡
公开(公告)号：WO02053779A3
公开(公告)日：2003-10-30
申请号：PCT/US0204583
申请日：2002-01-04
申请人： SAMUEL ROBERTS NOBLE FOUND INC , MAY GREGORY D , KMIEC ERIC B
发明人： MAY GREGORY D , KMIEC ERIC B
IPC分类号： A01H1/00 , C12N15/10 , C12N15/74 , C12N15/82 , C12Q1/68
CPC分类号： C12N15/102
摘要： An in vivo or in vitro cell-free method for genetic repair of mutation in plastid genes has been found which consists of (1) reacting a plasmid which contains a specific mutation (point mutation or frameshift mutation) of interest, a chimeric RNA/DNA oligonucleotide or a modified single stranded oligonucleotide which is believed to contain the genetic code for correcting the plastid gene mutation, and a chloroplast extract taken from the plant of interest, and (2) determining the success of gene conversion using a genetic readout system. A cell-free assay is disclosed by which the enzymatic capacity of chloroplast extracts to direct gene repair such as corrections to both point mutations and frameshift mutations can be determined. This assay method also enables the mechanistic study of plastid gene repair and facilitates the direct comparison between plant nuclear and organelle DNA repair pathways.
摘要翻译：已经发现体内或体外无细胞遗传修饰质体基因突变的方法已经被发现，其包括（1）使包含特定突变（点突变或移码突变）的目标质粒，嵌合RNA / DNA 被认为含有用于校正质体基因突变的遗传密码的寡核苷酸或修饰的单链寡核苷酸，以及从感兴趣的植物获取的叶绿体提取物，和（2）使用遗传读出系统确定基因转化的成功。公开了无细胞测定法，通过该测定可以确定叶绿体提取物的指导基因修复的酶能力，例如对两个突变和移码突变的校正。该测定方法还能够进行质体基因修复的机械研究，并促进植物核和细胞器质DNA修复途径之间的直接比较。

10. 发明申请

WO2003054217A3 PLANT MSH2 SEQUENCES AND METHODS OF USE 审中-公开
公开(公告)号：WO2003054217A3
公开(公告)日：2003-07-03
申请号：PCT/US2002/040887
申请日：2002-12-20
申请人： BOYCE THOMPSON INSTITUTE FOR PLANT RESEARCH , PIONEER HI-BRED INTERNATIONAL, INC. , KIPP, Peter, B. , MAY, Gregory, D. , MAHAJAN, Pradmod, B. , BASZCZYNSKI, Christopher, L. , ZHU, Tong
发明人： KIPP, Peter, B. , MAY, Gregory, D. , MAHAJAN, Pradmod, B. , BASZCZYNSKI, Christopher, L. , ZHU, Tong
IPC分类号： C12N15/29
摘要： The invention relates to isolated nucleic acid molecules encoding MutS homologues (MSHs). Such MSH proteins are involved in DNA mismatch-repair processes in organisms. The invention provides isolated nucleic acid molecules comprising MSH2 nucleotide sequences which encode MSH2 proteins and MSH2 nucloetide sequences which encode dominant-negative MSH2 variants. Such MSH2 nucleotide sequences find use in altering mismatch repair, mutation rates and recombination frequencies in both eukaryotic and prokaryotic organisms. The invention also provides isolated nucleic acid molecules comprising MSH2 promoter nucleotide sequences. Such MSH2 promoter nucleotide sequences find use in regulating the expression of genes of interest in plants. Additionally provided are isolated proteins, transformed host cells, and transformed plants, tissues, cells and seeds thereof.

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