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1. 发明专利

AU2006218625A1 Process and genes for expression and over-expression of active (FeFe) hydrogenases 未知
公开(公告)号：AU2006218625A1
公开(公告)日：2006-09-08
申请号：AU2006218625
申请日：2006-02-28
申请人： MIDWEST RESEARCH INST , SHARON SMOLINSKI , MATTHEW POSEWITZ , MARIA GHIRARDI , PAUL KING
发明人： SEIBERT MICHAEL
IPC分类号： C12P21/00 , C12N1/20 , C12N1/21
摘要： A process for expression of active [FeFe]-hydrogenase in a host organism that does not contain either the structural gene(s) for [FeFe]-hydrogenases and/or homologues for the maturation genes HydE, HydF and HyG, comprising: cloning the structural hydrogenase gene(s) and/or the maturation genes HydE, HydF and HydG from an organisms that contains these genes into expression plasmids; transferring the plasmids into an organism that lacks a native [FeFe]-hydrogenase or that has a disrupted [FeFe]-hydrogenase and culturing it aerobically; and inducing anaerobiosis to provide [FeFe] hydrogenase biosynthesis and H?2#191 production.

2. 发明授权

US08702925B2 Polarographic instrument system for oxygen and hydrogen measurement 有权
标题翻译：用于氧气和氢气测量的极谱仪器系统
公开(公告)号：US08702925B2
公开(公告)日：2014-04-22
申请号：US13108960
申请日：2011-05-16
申请人： Devin Karns , Jonathan Meuser , Matthew Posewitz , Edward Dempsey
发明人： Devin Karns , Jonathan Meuser , Matthew Posewitz , Edward Dempsey
IPC分类号： G01N27/26
CPC分类号： G01N27/48
摘要： A system for measuring hydrogen (H2) and oxygen (O2) including a sample system, a signal processing and system control device, at least one polarograph device and a computing device equipped with software for environmental and biological data analysis incorporating both user-defined and environmental data acquired by the signal processing and system control device. The system measures gas level or exchange (production and/or consumption) in the field or laboratory. In some embodiments, the system uses two Clark-type polarograph devices oppositely polarized with gas-specific polarographic circuits, wherein one polarograph device measures H2 gas and the other measures O2 gas. In some embodiments, the sample system includes an environmentally-controlled sample housing for accepting the polarograph devices and holding a test sample in a reservoir. The system measures H2 or O2 exchange in microbial samples, including microbial phototrophs which couple light harvesting to H2 and/or O2 exchange.
摘要翻译：一种用于测量氢（H2）和氧（O2）的系统，包括样品系统，信号处理和系统控制装置，至少一个极谱仪装置和装备有用于环境和生物数据分析的软件的计算装置，其结合用户定义和由信号处理和系统控制装置采集的环境数据。该系统测量田间或实验室的天然气水平或交换（生产和/或消费）。在一些实施例中，系统使用与气体特异性极谱法电路相反极化的两个Clark型极谱仪装置，其中一个极谱仪装置测量H2气体，另一个测量O2气体。在一些实施方案中，样品系统包括用于接收极谱法装置并将测试样品保持在储存器中的环境受控样品壳体。该系统测量微生物样品中的H2或O2交换，包括将光捕获耦合到H2和/或O2交换的微生物光合营养。

3. 发明申请

US20130102040A1 USE OF ENDOGENOUS PROMOTERS IN GENETIC ENGINEERING OF NANNOCHLOROPSIS GADITANA 有权
标题翻译：内源性促进剂在NANNOCHLOROPSIS GADITANA遗传工程中的应用
公开(公告)号：US20130102040A1
公开(公告)日：2013-04-25
申请号：US13654347
申请日：2012-10-17
申请人： Randor Radakovits , Robert Jinkerson , Matthew Posewitz
发明人： Randor Radakovits , Robert Jinkerson , Matthew Posewitz
IPC分类号： C12N15/79
CPC分类号： C12N15/79 , C07K14/405 , C12N1/12
摘要： The present disclosure is directed to novel polynucleotide sequences for use in Nannochloropsis gaditana. The novel polynucleotide sequences include control sequences and coding sequences. Also disclosed are novel gene expression constructs wherein N. gaditana promoters/control regions are operatively linked to N. gaditana or non-N. gaditana coding sequences. These novel polynucleotide sequences and expression constructs can be introduced into N. gaditana and can recombine into the N. gaditana genome. Expression from these polynucleotide sequences and expression constructs can enhance N. gaditana biomass and/or lipid biosynthesis. Also disclosed are methods for modifying N. gaditana, for example by stably transforming N. gaditana with nucleic acid sequences, growing the modified N. gaditana, and obtaining biomass and biofuels from the modified N. gaditana.
摘要翻译：本公开涉及用于Nannochloropsis gaditana的新型多核苷酸序列。新颖的多核苷酸序列包括控制序列和编码序列。还公开了新颖的基因表达构建体，其中N. gaditana启动子/控制区可操作地连接到N. gaditana或非N。 gad ana编码序列。这些新的多核苷酸序列和表达构建体可以被引入gad ana ana，并可以重组进gad ana基因组。来自这些多核苷酸序列和表达构建体的表达可以增强gad ana生物量和/或脂质生物合成。还公开了用于修饰N.agitana的方法，例如通过用核酸序列稳定转化N. gaditana，生长修饰的N. gaditana，以及从修饰的N. gaditana获得生物质和生物燃料。

4. 发明申请

US20120085642A1 POLAROGRAPHIC INSTRUMENT SYSTEM FOR OXYGEN AND HYDROGEN MEASUREMENT 有权
标题翻译：用于氧气和氢气测量的极化仪器系统
公开(公告)号：US20120085642A1
公开(公告)日：2012-04-12
申请号：US13108960
申请日：2011-05-16
申请人： Devin Karns , Jonathan Meuser , Matthew Posewitz , Edward Dempsey
发明人： Devin Karns , Jonathan Meuser , Matthew Posewitz , Edward Dempsey
IPC分类号： G01N33/50
CPC分类号： G01N27/48
摘要： A system for measuring hydrogen (H2) and oxygen (O2) including a sample system, a signal processing and system control device, at least one polarograph device and a computing device equipped with software for environmental and biological data analysis incorporating both user-defined and environmental data acquired by the signal processing and system control device. The system measures gas level or exchange (production and/or consumption) in the field or laboratory. In some embodiments, the system uses two Clark-type polarograph devices oppositely polarized with gas-specific polarographic circuits, wherein one polarograph device measures H2 gas and the other measures O2 gas. In some embodiments, the sample system includes an environmentally-controlled sample housing for accepting the polarograph devices and holding a test sample in a reservoir. The system measures H2 or O2 exchange in microbial samples, including microbial phototrophs which couple light harvesting to H2 and/or O2 exchange.
摘要翻译：一种用于测量氢（H2）和氧（O2）的系统，包括样品系统，信号处理和系统控制装置，至少一个极谱仪装置和装备有用于环境和生物数据分析的软件的计算装置，其结合用户定义和由信号处理和系统控制装置采集的环境数据。该系统测量田间或实验室的天然气水平或交换（生产和/或消费）。在一些实施例中，系统使用与气体特异性极谱法电路相反极化的两个Clark型极谱仪装置，其中一个极谱仪装置测量H2气体，另一个测量O2气体。在一些实施方案中，样品系统包括用于接收极谱法装置并将测试样品保持在储存器中的环境受控样品壳体。该系统测量微生物样品中的H2或O2交换，包括将光捕获耦合到H2和/或O2交换的微生物光合营养。

5. 发明申请

US20110059863A1 IN VIVO EVOLUTION OF HYDROGENASES USING A HYDROGEN-SENSING SYSTEM 审中-公开
标题翻译：使用氢传感系统进行水力发展
公开(公告)号：US20110059863A1
公开(公告)日：2011-03-10
申请号：US12918182
申请日：2009-03-10
申请人： Maria Lucia Ghirardi , Matt Wecker , Matthew Posewitz
发明人： Maria Lucia Ghirardi , Matt Wecker , Matthew Posewitz
IPC分类号： C40B30/08 , C12Q1/26 , C12N15/63 , C12N1/00
CPC分类号： C12Q1/6897 , C12N9/0067 , C12N9/0095 , C12P3/00 , C12Q1/02 , C12Q1/26 , G01N2333/90238
摘要： Provided herein are methods for measuring H2 production by a hydrogenase. Also provided are methods for evolving a hydrogenase or hydrogen-sensing system by comparing the level of production of H2 by the hydrogenase to the level Of H2 produced by a wild-type hydrogenase cultured under the same conditions and selecting a microorganism with increased H2 production over the H2 production by a microorganism having a wild-type hydrogenase. Further provided herein are microorganisms and plasmids comprising a hupSL promoter coupled with a reporter gene.
摘要翻译：本文提供了通过氢化酶测量H2产生的方法。还提供了通过将氢化酶的H 2生成水平与在相同条件下培养的野生型氢化酶产生的H 2水平进行比较来选择氢化酶或氢感测系统的方法，并选择具有增加的H 2产生的微生物由具有野生型氢化酶的微生物产生H2。本文还提供了微生物和质粒，其包含与报道基因偶联的hupSL启动子。

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