专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明授权

US06913679B1 Apparatus and methods for high resolution separation of sample components on microfabricated channel devices 有权
标题翻译：用于在微细化通道器件上高分辨率分离样品组分的装置和方法
公开(公告)号：US06913679B1
公开(公告)日：2005-07-05
申请号：US09249274
申请日：1999-02-11
申请人： Richard A. Mathies , Brian Paegel , Peter C. Simpson , Lester Hutt
发明人： Richard A. Mathies , Brian Paegel , Peter C. Simpson , Lester Hutt
IPC分类号： G01N21/64 , C12M1/00 , C12N15/09 , G01N27/447 , G01N37/00 , B01L3/02 , G01N27/453 , G01N30/02
CPC分类号： G01N27/44791
摘要： Sample component separation apparatus and methods are described. An exemplary sample component separation apparatus includes a separation channel having a turn portion configured to reduce band-broadening caused by passage of a sample through the turn portion. To reduce band broadening caused by passage of a sample through a turn portion, the turn portion may be constructed and arranged to have a sample transport characteristic that is different from the corresponding sample transport characteristic of a substantially straight portion of the separation channel. For example, the turn portion may be configured with an effective channel width that is smaller than the effective channel widths of the substantially straight portion of the separation channel. The actual channel width of the turn portion may be smaller than the channel widths of the substantially straight portion; the effective channel width of the turn portion may be reduced by placing one or more sample transport barriers or constrictions in the turn portion of the channel. Alternatively, the sample velocity through the turn portion may be controlled so as to reduce band broadening. For example, sample transport barriers may be disposed in the turn portion so that sample components of a given band travel through the turn portion at substantially the same effective rate, whereby the band orientation remains substantially aligned along radial directions characteristic of the turn portion. Other a sample transport characteristics, such as electrical resistance or fluid flow resistance, of the turn portion may be adapted to reduce band broadening caused by passage of the sample through the turn portion.
摘要翻译：描述了样品组分分离装置和方法。示例性部件分离装置包括分离通道，该分离通道具有弯曲部分，其被配置为减少由于样品通过转弯部分而导致的带宽变形。为了减少由于样品通过转弯部分而导致的带宽增加，转弯部分可以被构造和布置成具有不同于分离通道的基本直的部分的相应样品输送特性的样品输送特性。例如，转弯部分可以被配置为具有小于分离通道的基本上直的部分的有效通道宽度的有效通道宽度。转弯部分的实际通道宽度可以小于基本上直的部分的通道宽度; 可以通过在通道的转弯部分放置一个或多个样品输送屏障或收缩部来减小转弯部分的有效通道宽度。或者，可以控制通过转弯部分的采样速度，以便减小频带变宽。例如，样品输送屏障可以设置在转弯部分中，使得给定带的样品成分以基本上相同的有效速率行进通过转弯部分，由此带状取向保持基本上与转向部分的径向方向对准。转向部分的其它样品输送特性，例如电阻或流体阻力可以适于减少由于样品通过转弯部分而导致的带宽变宽。

IPRDB

热门服务

关于我们

友情链接

联系方式