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1. 发明申请

US20060281906A1 METHODS AND KITS FOR PURIFYING HIS-TAGGED PROTEINS 失效
标题翻译：用于纯化标记蛋白的方法和工具
公开(公告)号：US20060281906A1
公开(公告)日：2006-12-14
申请号：US11466274
申请日：2006-08-22
申请人： Tonny Johnson , Rebecca Godat , Laurie Engel
发明人： Tonny Johnson , Rebecca Godat , Laurie Engel
IPC分类号： C07K14/80
CPC分类号： B01J45/00 , C07K1/22
摘要： Disclosed are methods of separating a target molecule from a non-target molecule using zinc- or cobalt-charged solid supports.
摘要翻译：公开了使用带锌或钴的固体支持物从非靶分子分离靶分子的方法。

2. 发明申请

US20060281124A1 DEVICE AND METHOD FOR PURIFICATION OF BIOLOGICAL MATERIALS 审中-公开
标题翻译：用于净化生物材料的装置和方法
公开(公告)号：US20060281124A1
公开(公告)日：2006-12-14
申请号：US11466945
申请日：2006-08-24
申请人： Steven Ekenberg , Keith Wood , Laurie Engel
发明人： Steven Ekenberg , Keith Wood , Laurie Engel
IPC分类号： C12Q1/68 , C12M1/34 , G01N33/558
CPC分类号： C12N15/1017 , G01N33/54333
摘要： An apparatus, method and kit for isolating a biomolecule from a sample. The sample comprises a complex biological material, which includes insoluble matter. Some embodiments of the apparatus and kit include a reservoir and means for capturing the biomolecule either contained within or coupled to the reservoir. The reservoir can have an inner surface, and can be adapted to contain the sample. The apparatus can further include least one of a filter positioned between the means for capturing the biomolecule and at least a portion of the inner surface of the reservoir, and an aperture defined in the inner surface of the reservoir. Some embodiments of the method include combining the sample with a solid phase that is adapted to capture the biomolecule, removing the insoluble matter from the sample, and removing the biomolecule from the solid phase.
摘要翻译：用于从样品中分离生物分子的装置，方法和试剂盒。样品包括复杂的生物材料，其包括不溶性物质。装置和试剂盒的一些实施方案包括储存器和用于捕获包含在储存器中或耦合到储存器的生物分子的装置。储存器可以具有内表面，并且可以适于容纳样品。设备还可以包括位于用于捕获生物分子的装置和储存器的内表面的至少一部分之间的过滤器中的至少一个以及限定在储存器的内表面中的孔。该方法的一些实施方案包括将样品与适于捕获生物分子的固相组合，从样品中除去不溶物质，以及从固相中除去生物分子。

3. 发明授权

US07339036B2 Methods and kits for purifying his-tagged proteins 失效
标题翻译：用于纯化其标记蛋白质的方法和试剂盒
公开(公告)号：US07339036B2
公开(公告)日：2008-03-04
申请号：US11466274
申请日：2006-08-22
申请人： Tonny Johnson , Rebecca Godat , Laurie Engel
发明人： Tonny Johnson , Rebecca Godat , Laurie Engel
IPC分类号： A23J1/00
CPC分类号： B01J45/00 , C07K1/22
摘要： Disclosed are methods of separating a target molecule from a non-target molecule using zinc- or cobalt-charged solid supports.
摘要翻译：公开了使用带锌或钴的固体支持物从非靶分子分离靶分子的方法。

4. 发明申请

US20060105391A1 Device and method for separating molecules 审中-公开
公开(公告)号：US20060105391A1
公开(公告)日：2006-05-18
申请号：US11029882
申请日：2005-01-05
申请人： Laurie Engel , Tonny Johnson
发明人： Laurie Engel , Tonny Johnson
IPC分类号： G01N33/53 , C12M1/34
CPC分类号： G01N33/5302 , B01L3/0275 , B01L3/50255 , B01L2200/0631 , B01L2300/0829 , B01L2400/049 , C12N15/1017 , G01N30/02 , G01N33/54333 , G01N33/54366 , G01N2030/8813 , B01D15/34
摘要： An apparatus and method for separating a biomolecule from one or more contaminants in a sample. The apparatus can include a unitary device comprising a plurality of fractionation devices, each reservoir comprising a filter, each reservoir adapted to contain a solid phase, the solid phase adapted to separate the biomolecule and the contaminant by fractionation. The filter can have an average pore size that allows the sample to pass therethrough while substantially preventing the solid phase from passing therethrough. The method can include moving the sample past the solid phase in each reservoir to separate the biomolecule from the contaminant by fractionation to obtain an isolated biomolecule.

5. 发明授权

US07319021B2 Cell lysis composition, methods of use, apparatus and kit 失效
标题翻译：细胞裂解组合物，使用方法，仪器和试剂盒
公开(公告)号：US07319021B2
公开(公告)日：2008-01-15
申请号：US10673054
申请日：2003-09-26
申请人： Laurie Engel , John W. Schultz , Tonny M. Johnson , Kristopher Zimmerman , Laura L. Bozek , Judith N. Stevens
发明人： Laurie Engel , John W. Schultz , Tonny M. Johnson , Kristopher Zimmerman , Laura L. Bozek , Judith N. Stevens
IPC分类号： C12P21/06 , C12P21/04 , A23J1/00
CPC分类号： B01D15/3804 , B01D15/361 , B01J20/28009 , C07K1/36 , C11D1/44 , C11D1/662 , C11D1/72 , C11D1/88 , C11D3/3719 , C11D3/381 , C11D3/38636 , C12N1/06
摘要： Cell lysis compositions, methods for extracting and isolating proteins and peptides from a host cells using the compositions, kits and apparatus for extracting and isolating protein and peptide molecules from host cells and for detecting for the presence of a protein or peptide. The composition allows for the extraction and isolation of proteins and peptides from host cells without the need for mechanical disruption and with or without isolation of the cells from cell medium. The composition includes at least one surfactant having a hydrophobic-lipophilic balance value in the range from about 11 to about 16; and at least one cell membrane altering compound.
摘要翻译：细胞裂解组合物，使用用于从宿主细胞中提取和分离蛋白质和肽分子并用于检测蛋白质或肽的存在的组合物，试剂盒和装置从宿主细胞中提取和分离蛋白质和肽的方法。该组合物允许从宿主细胞中提取和分离蛋白质和肽，而不需要机械破坏并且有或没有从细胞培养基中分离细胞。该组合物包括至少一种具有在约11至约16范围内的疏水 - 亲油平衡值的表面活性剂; 和至少一种细胞膜改变化合物。

6. 发明申请

US20070105154A1 Cell lysis composition, methods of use, apparatus, and kit 审中-公开
标题翻译：细胞裂解组合物，使用方法，仪器和试剂盒
公开(公告)号：US20070105154A1
公开(公告)日：2007-05-10
申请号：US11590252
申请日：2006-10-31
申请人： Laurie Engel , John Shultz , Tonny Johnson , Kristopher Zimmerman , Laura Bozek , Judith Stevens
发明人： Laurie Engel , John Shultz , Tonny Johnson , Kristopher Zimmerman , Laura Bozek , Judith Stevens
IPC分类号： C40B40/10 , C40B30/06 , C12P21/06
CPC分类号： B01D15/3804 , B01D15/361 , B01J20/28009 , C07K1/36 , C11D1/44 , C11D1/662 , C11D1/72 , C11D1/88 , C11D3/3719 , C11D3/381 , C11D3/38636 , C12N1/06
摘要： Cell lysis compositions, methods for extracting and isolating proteins and peptides from a host cells using the compositions, kits and apparatus for extracting and isolating protein and peptide molecules from host cells and for detecting for the presence of a protein or peptide. The composition allows for the extraction and isolation of proteins and peptides from host cells without the need for mechanical disruption and with or without isolation of the cells from cell medium. The composition includes at least one surfactant having a hydrophobic-lipophilic balance value in the range from about 11 to about 16; and at least one cell membrane altering compound.
摘要翻译：细胞裂解组合物，使用用于从宿主细胞中提取和分离蛋白质和肽分子并用于检测蛋白质或肽的存在的组合物，试剂盒和装置从宿主细胞中提取和分离蛋白质和肽的方法。该组合物允许从宿主细胞中提取和分离蛋白质和肽，而不需要机械破坏并且有或没有从细胞培养基中分离细胞。该组合物包括至少一种具有在约11至约16范围内的疏水 - 亲油平衡值的表面活性剂; 和至少一种细胞膜改变化合物。

7. 发明申请

US20060141537A1 Device and method for separating molecules 审中-公开
标题翻译：用于分离分子的装置和方法
公开(公告)号：US20060141537A1
公开(公告)日：2006-06-29
申请号：US11358519
申请日：2006-02-21
申请人： Laurie Engel , Tonny Johnson
发明人： Laurie Engel , Tonny Johnson
IPC分类号： G01N33/53
CPC分类号： G01N33/5302 , B01L3/0275 , B01L3/50255 , B01L2200/0631 , B01L2300/0829 , B01L2400/049 , C12N15/1017 , G01N30/02 , G01N33/54333 , G01N33/54366 , G01N2030/8813 , B01D15/34
摘要： An apparatus and method for separating a biomolecule from one or more contaminants in a sample. The apparatus can include a unitary device comprising a plurality of fractionation devices, each reservoir comprising a filter, each reservoir adapted to contain a solid phase, the solid phase adapted to separate the biomolecule and the contaminant by fractionation. The filter can have an average pore size that allows the sample to pass therethrough while substantially preventing the solid phase from passing therethrough. The method can include moving the sample past the solid phase in each reservoir to separate the biomolecule from the contaminant by fractionation to obtain an isolated biomolecule.
摘要翻译：一种用于将生物分子与样品中的一种或多种污染物分离的装置和方法。该装置可以包括包括多个分馏装置的单一装置，每个储存器包括过滤器，每个储存器适于容纳固相，固相适于通过分馏分离生物分子和污染物。过滤器可以具有允许样品通过的平均孔径，同时基本上防止固相通过。该方法可以包括将样品移动通过每个储存器中的固相以通过分馏将生物分子与污染物分离以获得分离的生物分子。

8. 发明申请

US20050209444A1 Methods and kits for purifying his-tagged proteins 失效
公开(公告)号：US20050209444A1
公开(公告)日：2005-09-22
申请号：US11032281
申请日：2005-01-10
申请人： Tonny Johnson , Rebecca Godat , Laurie Engel
发明人： Tonny Johnson , Rebecca Godat , Laurie Engel
IPC分类号： B01D15/38 , B01J45/00 , C07K1/14 , C07K1/22 , C07K1/16
CPC分类号： B01J45/00 , C07K1/22
摘要： Disclosed are methods of separating a target molecule from a non-target molecule using zinc- or cobalt-charged solid supports.

9. 发明授权

US07150973B2 Methods and kits for purifying his-tagged proteins 失效
标题翻译：用于纯化其标记蛋白质的方法和试剂盒
公开(公告)号：US07150973B2
公开(公告)日：2006-12-19
申请号：US11032281
申请日：2005-01-10
申请人： Tonny Johnson , Rebecca Godat , Laurie Engel
发明人： Tonny Johnson , Rebecca Godat , Laurie Engel
IPC分类号： G01N33/53 , C07K1/00 , C12P21/06
CPC分类号： B01J45/00 , C07K1/22
摘要： Disclosed are methods of separating a target molecule from a non-target molecule using zinc- or cobalt-charged solid supports.
摘要翻译：公开了使用带锌或钴的固体支持物从非靶分子分离靶分子的方法。

10. 发明授权

US07115397B2 Methods and kits for purifying his-tagged proteins 失效
公开(公告)号：US07115397B2
公开(公告)日：2006-10-03
申请号：US10840408
申请日：2004-05-06
申请人： Tonny Johnson , Rebecca Godat , Laurie Engel
发明人： Tonny Johnson , Rebecca Godat , Laurie Engel
IPC分类号： C12P21/06 , G01N33/53 , A61K38/00
CPC分类号： B01J45/00 , C07K1/22
摘要： Disclosed are methods of separating a target molecule from a non-target molecule using zinc- or cobalt-charged solid supports.

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