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1. 发明专利

AU5932301A Derivation of midbrain dopaminergic neurons from embryonic stem cells 未知
公开(公告)号：AU5932301A
公开(公告)日：2001-11-12
申请号：AU5932301
申请日：2001-05-01
申请人： US HEALTH , LEE SANG HUN , LUMELSKY NADYA , STUDER LORENZ , MCKAY RON D G
发明人： LEE SANG-HUN , LUMELSKY NADYA , STUDER LORENZ , MCKAY RON D G
IPC分类号： A61K35/12 , A61K48/00 , A61P25/00 , C12N5/0793 , C12N5/06 , A61K35/30 , C12N5/08 , C12N5/10 , C12Q1/02

2. 发明申请

WO02059278A3 DIFFERENTIATION OF STEM CELLS TO PANCREATIC ENDOCRINE CELLS 审中-公开
标题翻译：干细胞对胰腺内皮细胞的分化
公开(公告)号：WO02059278A3
公开(公告)日：2003-07-10
申请号：PCT/US0202361
申请日：2002-01-24
申请人： US GOV HEALTH & HUMAN SERV , LUMELSKY NADYA L , BLONDEL OLIVER , MC KAY RONALD D , KIM JONG-HOON
发明人： LUMELSKY NADYA L , BLONDEL OLIVER , MC KAY RONALD D , KIM JONG-HOON
IPC分类号： C12N15/09 , A61K35/12 , A61L27/00 , A61P3/10 , C12N5/071 , C12Q1/02 , C12N5/06 , C12N5/08
CPC分类号： C12N5/0677 , A61K35/12 , C12N2500/38 , C12N2501/115 , C12N2501/41 , C12N2503/02 , C12N2506/02 , C12N2510/02
摘要： A method is provided for differentiating embryonic stem cells to endocrine cells. The method includes generating embryoid bodies from a culture of undifferentiated embryonic stem cells, selecting endocrine precursor cells, expanding the endocrine precursor cells by culturing endocrine cells in an expansion medium that comprises a growth factor, and differentiating the expanded endocrine precursor cells in a differentiation media to differentiated endocrine cells produced by this method are also provided. Artificial islets are disclosed, as well as method for using the pancreatic endocrine cells and the artificial islets.
摘要翻译：提供了一种将胚胎干细胞分化为内分泌细胞的方法。该方法包括从未分化的胚胎干细胞的培养物中产生胚状体，选择内分泌前体细胞，通过在包含生长因子的扩张培养基中培养内分泌细胞来扩增内分泌前体细胞，并将扩增的内分泌前体细胞分化为分化培养基还提供了通过该方法产生的分化的内分泌细胞。公开了人造胰岛，以及使用胰内分泌细胞和人造胰岛的方法。

3. 发明申请

WO0183715A3 DERIVATION OF MIDBRAIN DOPAMINERGIC NEURONS FROM EMBRYONIC STEM CELLS 审中-公开
标题翻译：胚胎干细胞衍化中脑多巴胺能神经元
公开(公告)号：WO0183715A3
公开(公告)日：2002-05-23
申请号：PCT/US0114051
申请日：2001-05-01
申请人： US GOV HEALTH & HUMAN SERV , LEE SANG HUN , LUMELSKY NADYA , STUDER LORENZ , MCKAY RON D G
发明人： LEE SANG-HUN , LUMELSKY NADYA , STUDER LORENZ , MCKAY RON D G
IPC分类号： A61K35/12 , A61K48/00 , A61P25/00 , C12N5/0793 , C12N5/06 , A61K35/30 , C12N5/08 , C12N5/10 , C12Q1/02
CPC分类号： C12N5/0619 , A61K35/12 , A61K48/00 , C12N2501/115 , C12N2501/119 , C12N2501/41 , C12N2506/02
摘要： The invention provides a method of culturing cells. The method generally includes five stages: (1) expansion of ES cells; (2) generation of embryoid bodies; (3) selection of CNS precursor cells; (4) expansion of CNS precursor cells; and (5) differentiation of CNS precursor cells. During the expansion phase, the CNS precursor cells are cultured in a media which includes at least one neurologic agent such as bFGF, SHH, and FGF8. The expanded CNS precursors are differentiated by withdrawal of at least one neurologic agent, typically, bFGF. Preferably, the differentiation media includes ascorbic acid. The method of the invention can be used to culture a variety of cells, preferably neuronal cells, including, but not limited to dopaminergic neuron cells, cholinergic neuronal cells and serotonergic neuron cells. The invention also provides a method for treating a neurological disorder, such as Parkinson's disease, a method of introducing a gene product into a brain of a patient, and an assay for neurologically active substances. The invention further provides a cell culture which includes differentiated neuron cells, of which at least about 20 % of the differentiated neurons are dopaminergic neurons.
摘要翻译：本发明提供了培养细胞的方法。该方法通常包括五个阶段：（1）ES细胞的扩增; （2）胚状体的产生; （3）选择CNS前体细胞; （4）CNS前体细胞的扩增; 和（5）CNS前体细胞的分化。在扩展阶段期间，将CNS前体细胞在包含至少一种神经药剂如bFGF，SHH和FGF8的培养基中培养。扩大的CNS前体通过撤出至少一种神经药剂（通常为bFGF）而分化。优选地，分化培养基包含抗坏血酸。本发明的方法可用于培养各种细胞，优选神经元细胞，包括但不限于多巴胺能神经元细胞，胆碱能神经元细胞和5-羟色胺能神经元细胞。本发明还提供治疗神经障碍如帕金森病的方法，将基因产物导入患者脑内的方法和神经活性物质的检测方法。本发明进一步提供了包括分化的神经元细胞的细胞培养物，其中至少约20％的分化的神经元是多巴胺能神经元。

4. 发明申请

WO0183715A8 DERIVATION OF MIDBRAIN DOPAMINERGIC NEURONS FROM EMBRYONIC STEM CELLS 审中-公开
标题翻译：从胚胎干细胞衍生的中性多巴胺神经元
公开(公告)号：WO0183715A8
公开(公告)日：2002-12-27
申请号：PCT/US0114051
申请日：2001-05-01
申请人： US GOV HEALTH & HUMAN SERV , LEE SANG-HUN , LUMELSKY NADYA , STUDER LORENZ , MCKAY RON D G , AUERBACH JONATHAN , KIM JONG-HOON
发明人： LEE SANG-HUN , LUMELSKY NADYA , STUDER LORENZ , MCKAY RON D G , AUERBACH JONATHAN , KIM JONG-HOON
IPC分类号： A61K35/12 , A61K48/00 , A61P25/00 , C12N5/0793 , C12N5/06 , A61K35/30 , C12N5/08 , C12N5/10 , C12Q1/02
CPC分类号： C12N5/0619 , A61K35/12 , A61K48/00 , C12N2501/115 , C12N2501/119 , C12N2501/41 , C12N2506/02
摘要： The invention provides a method of culturing cells. The method generally includes five stages: (1) expansion of ES cells; (2) generation of embryoid bodies; (3) selection of CNS precursor cells; (4) expansion of CNS precursor cells; and (5) differentiation of CNS precursor cells. During the expansion phase, the CNS precursor cells are cultured in a media which includes at least one neurologic agent such as bFGF, SHH, and FGF8. The expanded CNS precursors are differentiated by withdrawal of at least one neurologic agent, typically, bFGF. Preferably, the differentiation media includes ascorbic acid. The method of the invention can be used to culture a variety of cells, preferably neuronal cells, including, but not limited to dopaminergic neuron cells, cholinergic neuronal cells and serotonergic neuron cells. The invention also provides a method for treating a neurological disorder, such as Parkinson's disease, a method of introducing a gene product into a brain of a patient, and an assay for neurologically active substances. The invention further provides a cell culture which includes differentiated neuron cells, of which at least about 20 % of the differentiated neurons are dopaminergic neurons.
摘要翻译：本发明提供培养细胞的方法。该方法一般包括五个阶段：（1）ES细胞扩增; （2）胚状体的产生; （3）CNS前体细胞的选择; （4）CNS前体细胞的扩增; 和（5）CNS前体细胞的分化。在扩增阶段期间，CNS前体细胞在包含至少一种神经病学试剂如bFGF，SHH和FGF8的培养基中培养。扩展的CNS前体通过至少一种神经病药物（通常为bFGF）的撤出来区分。优选地，分化培养基包括抗坏血酸。本发明的方法可用于培养各种细胞，优选神经元细胞，包括但不限于多巴胺能神经元细胞，胆碱能神经元细胞和血清素能神经元细胞。本发明还提供了治疗诸如帕金森病的神经障碍的方法，将基因产物引入患者脑中的方法和神经活性物质的测定法。本发明还提供了包含分化的神经元细胞的细胞培养物，其中至少约20％的分化的神经元是多巴胺能神经元。

5. 发明申请

WO2002059278A2 DIFFERENTIATION OF STEM CELLS TO PANCREATIC ENDOCRINE CELLS 审中-公开
标题翻译：干细胞对胰腺内皮细胞的分化
公开(公告)号：WO2002059278A2
公开(公告)日：2002-08-01
申请号：PCT/US2002/002361
申请日：2002-01-24
申请人： THE GOVERNMENT OF THE UNITED STATES OF AMERICA, as represented by THE SECRETARY OF DEPARTMENT OF HEALTH & HUMAN SERVICES , LUMELSKY, Nadya, L. , BLONDEL, Oliver , MC KAY, Ronald, D. , KIM, Jong-Hoon
发明人： LUMELSKY, Nadya, L. , BLONDEL, Oliver , MC KAY, Ronald, D. , KIM, Jong-Hoon
IPC分类号： C12N5/00
CPC分类号： C12N5/0677 , A61K35/12 , C12N2500/38 , C12N2501/115 , C12N2501/41 , C12N2503/02 , C12N2506/02 , C12N2510/02
摘要： A method is provided for differentiating embryonic stem cells to endocrine cells. The method includes generating embryoid bodies from a culture of undifferentiated embryonic stem cells, selecting endocrine precursor cells, expanding the endocrine precursor cells by culturing endocrine cells in an expansion medium that comprises a growth factor, and differentiating the expanded endocrine precursor cells in a differentiation media to differentiated endocrine cells produced by this method are also provided. Artificial islets are disclosed, as well as method for using the pancreatic endocrine cells and the artificial islets.
摘要翻译：提供了一种将胚胎干细胞分化为内分泌细胞的方法。该方法包括从未分化的胚胎干细胞的培养物中产生胚状体，选择内分泌前体细胞，通过在包含生长因子的扩张培养基中培养内分泌细胞来扩增内分泌前体细胞，并将扩增的内分泌前体细胞分化为分化培养基还提供了通过该方法产生的分化的内分泌细胞。公开了人造胰岛，以及使用胰内分泌细胞和人造胰岛的方法。

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