专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

2. 发明申请

WO1998029730A1 METHOD AND APPARATUS FOR SELECTIVE DEPLETION OF BIOLOGICAL PARTICLES 审中-公开
标题翻译：选择性去除生物颗粒的方法和装置
公开(公告)号：WO1998029730A1
公开(公告)日：1998-07-09
申请号：PCT/US1997023290
申请日：1997-12-18
申请人： RORER PHARMACEUTICAL PRODUCTS INC. , FAMA, Lawrence, J. , LAMONS, Donald , SCHOENBERG, Stephen
发明人： RORER PHARMACEUTICAL PRODUCTS INC.
IPC分类号： G01N15/02
CPC分类号： C12N5/0081 , G01N1/405 , G01N33/54366 , G01N33/56966
摘要： A method and apparatus for effecting depletion of selected cells from a mixture of cells is disclosed. The device includes a plurality of vertically oriented binding chambers, each capable of containing biologically active receptor material such as polystyrene-fiber pads having "receptor" antibodies covalently bound to the fibers. A valve system is employed which allows the user of the device to route the cell mixture and rinsing fluids into and out of the binding chambers according to a preselected sequence. The flow of the cell mixture is such that it flows from the bottom of each chamber to the top of the chamber, pushed upward by hydrostatic pressure. Automatic cut-off means, such as hydrophobic valves, automatically terminate the flow of the cell mixture into the binding chambers when the level of the cell mixture reaches a predetermined point in the binding chambers. A rinse material, such as phosphate buffered saline, is selectively routed into the binding chambers from the top of the chambers. Optimal results are achieved by introducing the rinse fluid to the chambers at a specific flow rate. The first binding step is performed on cellular material of relatively high concentration and the second binding step is performed after substantially diluting the depleted cellular material produced during the first binding step.
摘要翻译：公开了一种用于从细胞混合物中消耗所选择的细胞的方法和装置。该装置包括多个垂直定向的结合室，每个结合室能够包含生物活性受体材料，例如具有与纤维共价结合的“受体”抗体的聚苯乙烯纤维垫。使用阀系统，其允许装置的使用者根据预先选择的顺序将细胞混合物和冲洗液体进入和离开装订室。电池混合物的流动使得其从每个室的底部流到室的顶部，由静水压力向上推动。当细胞混合物的水平达到装订室中的预定点时，诸如疏水阀的自动切断装置自动终止细胞混合物流入结合室中。诸如磷酸盐缓冲盐水的冲洗材料从室的顶部选择性地引导到装订室中。通过以特定流速将冲洗流体引入腔室来实现最佳结果。第一结合步骤在相对高浓度的细胞材料上进行，第二结合步骤在基本上稀释在第一结合步骤期间产生的耗尽的细胞材料之后进行。

IPRDB

热门服务

关于我们

友情链接

联系方式