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    • 5. 发明授权
    • Method for electroporation of biological cells
    • 生物细胞电穿孔方法
    • US08465955B2
    • 2013-06-18
    • US12072016
    • 2008-02-22
    • Ulrich ZimmermannVladimir SukhorukovKurt Lucas
    • Ulrich ZimmermannVladimir SukhorukovKurt Lucas
    • C12M1/42C12N13/00
    • C12M35/02C12N13/00
    • Described are methods for electrical treatment of biological cells, in particular using electrical field pulses, involving the steps: arrangement of the cells (1) on apertures (2) of a solid planar carrier element (3) which divides a measuring chamber (10) into two compartments (11, 12); and temporary formation of an electrical treatment field which permeates the cells, wherein an alternating-current impedance measurement takes place on the carrier element (3), and from the result of the alternating-current impedance measurement, a degree of coverage of the carrier element and/or healing of the cells after electrical treatment are/is acquired. Also described are devices for implementing the methods.
    • 描述了用于电处理生物细胞的方法,特别是使用电场脉冲,涉及以下步骤:将细胞(1)布置在分隔测量室(10)的实心平面载体元件(3)的孔(2)上, 分成两个隔间(11,12); 并且临时形成渗透到电池的电处理场,其中在载体元件(3)上进行交流阻抗测量,并且根据交流阻抗测量的结果,载体元件的覆盖度 和/或获得电处理后的细胞愈合。 还描述了用于实现该方法的装置。