专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

US20080214417A1 Lubricant for wear testing of joint replacements and associated materials 审中-公开
公开(公告)号：US20080214417A1
公开(公告)日：2008-09-04
申请号：US12069280
申请日：2008-02-07
申请人： Jan-M Brandt , Kory D. Charron , Lin Zhao , John B. Medley , Steven J. MacDonald
发明人： Jan-M Brandt , Kory D. Charron , Lin Zhao , John B. Medley , Steven J. MacDonald
IPC分类号： C10M125/24
CPC分类号： A61K35/16 , C12N9/6462 , C12Y304/21073
摘要： The present invention relates to an artificial synovial fluid composition that mimics the tribological properties of human, osteoarthritic synovial fluid (SF) for the main purpose of in vitro wear testing. There is described an artificial synovial fluid composition comprising: a) a mammalian serum having i) a total protein concentration of from about 10 to about 45 g/L; ii) an albumin fraction of from about 55% to about 80% w/w %; iii) an α-1 globulin fraction of from about 3% to about 6% w/w %; iv) an α-2 globulin fraction of from about 5% to about 10% w/w %; v) a β-globulin fraction of from about 3% to about 20% w/w %; vi) a γ-globulin fraction of from about 5% to about 20% w/w %; vii) a calcium (Ca) concentration of from about 0.1 mmol/L to about 3 mmol/L; viii) a magnesium (Mg) concentration of from about 0.05 mmol/L to about 0.8 mmol/L; ix) an inorganic phosphate (P) concentration of from about 0.1 mmol/L to about 1.5 mmol/L; x) an iron (Fe) concentration of from about 0.001 mmol/L to about 0.1 mmol/L; xi) a peptide concentration at a 2,000 Da molecular weight cut-off of from about 0.005 g/L to about 10 g/L; xii) a maximal transition midpoint temperature, Tm-cp-max, of from about 330 K to about 350 K at a concentration molality of from about 0.005 to about 0.15 mmol/L measured at a scan rate of from about 20 K/hour to about 100 K/hour; xiii) a total enthalpy change, ΔH, of from about 650 kJ mol−1 to about 1200 kJ mol−1 at a set concentration molality of 0.05 mmol/l measured at a scan rate of 60 K/hour; xiv) a total entropy change, ΔS, of from about 1.5 kJ mol−1 K−1 to about 4 kJ mol−1 K−1 at a concentration molality of from about 0.005 mmol/l to about 0.15 mmol/l and a scan rate from about 20 K/hour to 100 K/hour; wherein the ratio of albumin to total globulin (α-1 globulin+α-2 globulin+β-globulin+γ-globulin fractions) in solution is from about 1.5 to about 3; b) a buffer for maintaining a solution osmolality of from about 200 mmol/kg to about 400 mmol/kg; c) hyaluronic acid (HA) at a concentration of from about 0.1 g/L to about 6 g/L; d) hyaluronic acid (HA) with a molecular weight of from 1 MDa to 4 MDa; and e) a pH level of about 7 to about 8.

IPRDB

热门服务

关于我们

友情链接

联系方式