专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明授权

US06573429B1 DNA fragment for stable expression of an exogenous gene in a plant 失效
标题翻译：用于在植物中稳定表达外源基因的DNA片段
公开(公告)号：US06573429B1
公开(公告)日：2003-06-03
申请号：US09711933
申请日：2000-11-15
申请人： Atsuhiko Shinmyo , Kazuya Yoshida , Ko Kato , Shingo Nagaya , Michio Shibata , Ryutaro Aida
发明人： Atsuhiko Shinmyo , Kazuya Yoshida , Ko Kato , Shingo Nagaya , Michio Shibata , Ryutaro Aida
IPC分类号： A01H100
CPC分类号： C12N15/822
摘要： A method useful for stable expression of an exogenous gene, introduced into a plant, is provided. By introducing an exogenous gene and 5′ upstream sequence of tobacco dehydogenase concurrently, stable expression of the exogenous gene introduced into a plant can be achieved.
摘要翻译：提供了可用于稳定表达引入植物中的外源基因的方法。通过同时引入烟草脱氢酶的外源基因和5'上游序列，可以实现引入植物的外源基因的稳定表达。

2. 发明授权

US09163254B2 Recombinant DNA molecule encoding 5′ UTR capable of preventing inhibition of translation under environmental stresses 有权
标题翻译：编码5'UTR的重组DNA分子能够防止环境胁迫下的翻译抑制
公开(公告)号：US09163254B2
公开(公告)日：2015-10-20
申请号：US13391069
申请日：2010-08-19
申请人： Ko Kato , Shigehiko Kanaya , Hideyuki Matsuura , Shinya Takenami , Tatsuya Nosho , Yuhki Kubo , Kiyotaka Ueda
发明人： Ko Kato , Shigehiko Kanaya , Hideyuki Matsuura , Shinya Takenami , Tatsuya Nosho , Yuhki Kubo , Kiyotaka Ueda
IPC分类号： C12N15/82 , C12N15/67 , C12N15/113
CPC分类号： C12N15/8273 , C12N15/113 , C12N15/67 , C12N15/8216
摘要： An object of the present invention is to identify sequence features of the 5′ UTR, which are involved in changes in the translational state of plants under environmental stresses, and to provide a recombinant DNA molecule, an expression vector, a transformant, and the like, having the above sequence features. The present invention provides a recombinant DNA molecule encoding mRNA containing the 5′ UTR (a) or (b) defined below, a vector obtained by ligating the recombinant DNA molecule to a site immediately downstream of the transcription initiation point of a promoter, and a transformant comprising the vector:(a) 5′ UTR in which a sequence of bases 1 to 7 and a sequence of bases 12 to 32 from the 5′ end are, respectively, a sequence of bases 1 to 7 and a sequence of bases 12 to 32 of SEQ ID NO: 4, 6, 20, 36, or 60; and(b) 5′ UTR in which one or more bases in the base sequence of the 5′ UTR of (a) are replaced, and which escapes translational repression induced by at least one environmental stress selected from the group consisting of heat stress and salt stress.
摘要翻译：本发明的一个目的是鉴定参与环境胁迫下植物的翻译状态变化的5'UTR的序列特征，并提供重组DNA分子，表达载体，转化体等具有上述序列特征。本发明提供了编码含有下述定义的5'UTR（a）或（b）的mRNA的重组DNA分子，通过将重组DNA分子连接到启动子的转录起始点的紧邻位点而获得的载体，包含载体的转化体：（a）5'UTR，其中5'端的碱基1至7的序列和碱基12至32的序列分别是碱基序列1至7和碱基序列12 至32，SEQ ID NO：4,6,20,36或60; 和（b）其中（a）的5'UTR的碱基序列中的一个或多个碱基被替换的5'UTR，并且其逃避由至少一种环境应力诱导的平移抑制，所述环境应力选自热应激和盐胁迫

3. 发明授权

US06229070B1 Method of stable gene expression in a transgenic plant utilizing an insulator nucleotide sequence from the sea urchin arylsulfatase gene 失效
标题翻译：利用海胆芳基硫酸酯酶基因的绝缘体核苷酸序列，在转基因植物中稳定表达基因的方法
公开(公告)号：US06229070B1
公开(公告)日：2001-05-08
申请号：US09444570
申请日：1999-11-19
申请人： Atsuhiko Shinmyo , Kazuya Yoshida , Ko Kato , Koji Akasaka , Takaaki Kusumi , Yoshikazu Tanaka
发明人： Atsuhiko Shinmyo , Kazuya Yoshida , Ko Kato , Koji Akasaka , Takaaki Kusumi , Yoshikazu Tanaka
IPC分类号： A01H500
CPC分类号： C12N15/822 , C12N15/8216
摘要： A method for the stable expression of an introduced exogenous gene in a plant or plant cell is provided. Stable expression of an exogenous gene that was introduced was achieved by operably linking an upstream sequence of sea urchin arylsulfatase gene as an insulator.
摘要翻译：提供了在植物或植物细胞中稳定表达引入的外源基因的方法。引入的外源基因的稳定表达是通过可靠地连接上游的海胆芳基硫酸酯酶基因序列作为绝缘体来实现的。

4. 发明申请

US20090114255A1 CLEANING APPARATUS AND AUTOMATIC ANALYZER 审中-公开
标题翻译：清洁装置和自动分析仪
公开(公告)号：US20090114255A1
公开(公告)日：2009-05-07
申请号：US12272397
申请日：2008-11-17
申请人： Ko KATO
发明人： Ko KATO
IPC分类号： B08B3/10 , B08B3/04
CPC分类号： B01L13/02 , B01L3/021 , B01L3/5082 , B01L2200/146 , B01L2400/0487 , G01N2035/00564 , G01N2035/0437 , G01N2035/1025
摘要： A cleaning apparatus includes a plurality of air nozzles that discharge air from above each of the distal ends of a suction nozzle and a discharge nozzle, the suction nozzle sucking in at least some of reaction liquid undergoing a heterogeneous reaction, the discharge nozzle discharging a predetermined B/F cleaning fluid, the air nozzles being provided corresponding respectively to a plurality of pairs of the suction and the discharge nozzles. The apparatus includes an air supply unit that supplies air to the plurality of air nozzles at a time; a plurality of pressure sensors each of which detects a pressure of air which is supplied by the air supply unit and which is in the vicinity of each proximal end of the air nozzles; and a control unit that individually controls the B/F-cleaning-fluid discharging operation of the discharge nozzles based on the respective detected pressures and changes in the pressures.
摘要翻译：清洁装置包括从吸嘴和排出喷嘴的每个远端上方排出空气的多个空气喷嘴，吸入喷嘴吸入至少一些经历不均匀反应的反应液体，排出喷嘴排出预定的 B / F清洗流体，空气喷嘴分别对应于多对吸引和排出喷嘴。该装置包括一次向多个空气喷嘴供给空气的空气供给单元; 多个压力传感器，每个压力传感器检测由空气供应单元供应并且在空气喷嘴的每个近端附近的空气的压力; 以及控制单元，其基于相应的检测压力和压力变化分别控制排出喷嘴的B / F清洗流体排出操作。

5. 发明申请

US20060257988A1 Chlorohydrin and hydroxycarboxylic ester asymmetric hydrolase gene 失效
标题翻译：氯醇和羟基羧酸酯不对称水解酶基因
公开(公告)号：US20060257988A1
公开(公告)日：2006-11-16
申请号：US11490749
申请日：2006-07-20
申请人： Atsushi Nakagawa , Toshio Suzuki , Atsuhiko Shinmyo , Ko Kato , Hideaki Idogaki
发明人： Atsushi Nakagawa , Toshio Suzuki , Atsuhiko Shinmyo , Ko Kato , Hideaki Idogaki
IPC分类号： C12N9/18
CPC分类号： C12P41/005 , C07K2319/02 , C12N9/18 , C12P7/42 , C12P7/62 , C12P17/04
摘要： The present invention is to provide a gene having asymmetric hydrolase activity which is useful for synthesis of an optically active carboxylic acid, its antipode ester, and lactone, and a hydroxycarboxylic ester asymmetric hydrolase enzyme (EnHCH) derived from Enterobacter sp. DS-S-75 strain (FERM BP-5494) which is bacteria belonging to the genus Enterobacter, a EnHCH gene shown by base sequence of SEQ. ID. NO: 1, a gene encoding a protein having an amino acid sequence of SEQ. ID. NO: 2, and E. coli DH5α (pKK-EnHCH) deposited to International Patent Organism Depositary, National Institute of Advanced Industrial Science and Technology as a deposition No. FERM BP-08466.
摘要翻译：本发明提供具有不对称水解酶活性的基因，其可用于合成光学活性羧酸，其对映体酯和内酯，以及衍生自肠杆菌属的羟基羧酸酯不对称水解酶（EnHCH）。属于肠杆菌属的细菌DS-S-75菌株（FERM BP-5494），由SEQ ID NO：1的碱基序列显示的EnHCH基因。 ID。 NO：1，编码具有SEQ ID NO：1的氨基酸序列的蛋白质的基因。 ID。 NO：2和大肠杆菌DH5α（pKK-EnHCH），保藏在国际专利生物保藏中心，国立先进工业科学技术研究所作为FERM BP-08466。

6. 发明授权

US07112428B2 Chlorohydrin and hydroxycarboxylic ester asymmetric hydrolase gene 有权
标题翻译：氯醇和羟基羧酸酯不对称水解酶基因
公开(公告)号：US07112428B2
公开(公告)日：2006-09-26
申请号：US10680024
申请日：2003-10-07
申请人： Atsushi Nakagawa , Toshio Suzuki , Atsuhiko Shinmyo , Ko Kato , Hideaki Idogaki
发明人： Atsushi Nakagawa , Toshio Suzuki , Atsuhiko Shinmyo , Ko Kato , Hideaki Idogaki
IPC分类号： C12N9/18 , C12N5/00 , C12N1/20 , C12P21/06 , C12P7/42 , C07H21/04
CPC分类号： C12P41/005 , C07K2319/02 , C12N9/18 , C12P7/42 , C12P7/62 , C12P17/04
摘要： The present invention is to provide a gene having asymmetric hydrolase activity which is useful for synthesis of an optically active carboxylic acid, its antipode ester, and lactone, and a hydroxycarboxylic ester asymmetric hydrolase enzyme (EnHCH) derived from Enterobacter sp. DS-S-75 strain (FERM BP-5494) which is bacteria belonging to the genus Enterobacter, a EnHCH gene shown by base sequence of SEQ.ID.NO: 1, a gene encoding a protein having an amino acid sequence of SEQ.ID.NO: 2, and E. coli DH5α (pKK-EnHCH) deposited to International Patent Organism Depositary, National Institute of Advanced Industrial Science and Technology as a deposition No. FERM BP-08466.
摘要翻译：本发明提供具有不对称水解酶活性的基因，其可用于合成光学活性羧酸，其对映体酯和内酯，以及衍生自肠杆菌属的羟基羧酸酯不对称水解酶（EnHCH）。属于肠杆菌属的细菌的DS-S-75菌株（FERM BP-5494），SEQ ID NO：1的碱基序列所示的EnHCH基因，编码具有SEQ ID NO：1的氨基酸序列的蛋白质的基因。 ID.NO:2和大肠杆菌DH5α（pKK-EnHCH），保藏在国际专利生物保藏中心，国立先进工业科学技术研究所作为FERM BP-08466。

7. 发明申请

US20120174256A1 RECOMBINANT DNA MOLECULE ENCODING 5' UTR CAPABLE OF PREVENTING INHIBITION OF TRANSLATION UNDER ENVIRONMENTAL STRESSES 有权
标题翻译：重组DNA分子编码5'端能防止环境胁迫下翻译的抑制
公开(公告)号：US20120174256A1
公开(公告)日：2012-07-05
申请号：US13391069
申请日：2010-08-19
申请人： Ko Kato , Shigehiko Kanaya , Hideyuki Matsuura , Shinya Takenami , Tatsuya Nosho , Yuhki Kubo , Kiyotaka Ueda
发明人： Ko Kato , Shigehiko Kanaya , Hideyuki Matsuura , Shinya Takenami , Tatsuya Nosho , Yuhki Kubo , Kiyotaka Ueda
IPC分类号： A01H5/00 , C12N15/82 , G06F17/10 , A01H1/06 , C07H1/00 , C07H21/02 , C12N15/11 , C12P21/00
CPC分类号： C12N15/8273 , C12N15/113 , C12N15/67 , C12N15/8216
摘要： An object of the present invention is to identify sequence features of the 5′ UTR, which are involved in changes in the translational state of plants under environmental stresses, and to provide a recombinant DNA molecule, an expression vector, a transformant, and the like, having the above sequence features. The present invention provides a recombinant DNA molecule encoding mRNA containing the 5′ UTR (a) or (b) defined below, a vector obtained by ligating the recombinant DNA molecule to a site immediately downstream of the transcription initiation point of a promoter, and a transformant comprising the vector:(a) 5′ UTR in which a sequence of bases 1 to 7 and a sequence of bases 12 to 32 from the 5′ end are, respectively, a sequence of bases 1 to 7 and a sequence of bases 12 to 32 of SEQ ID NO: 4, 6, 20, 36, or 60; and (b) 5′ UTR in which one or more bases in the base sequence of the 5′ UTR of (a) are replaced, and which escapes translational repression induced by at least one environmental stress selected from the group consisting of heat stress and salt stress.
摘要翻译：本发明的一个目的是鉴定参与环境胁迫下植物的翻译状态变化的5'UTR的序列特征，并提供重组DNA分子，表达载体，转化体等具有上述序列特征。本发明提供了编码含有下述定义的5'UTR（a）或（b）的mRNA的重组DNA分子，通过将重组DNA分子连接到启动子的转录起始点的紧邻位点而获得的载体，包含载体的转化体：（a）5'UTR，其中5'端的碱基1至7的序列和碱基12至32的序列分别是碱基序列1至7和碱基序列12 至32，SEQ ID NO：4,6,20,36或60; 和（b）其中（a）的5'UTR的碱基序列中的一个或多个碱基被替换的5'UTR，并且其逃避由至少一种环境应力诱导的平移抑制，所述环境应力选自热应激和盐胁迫

8. 发明授权

US07335502B2 Chlorohydrin and hydroxycarboxylic ester asymmetric hydrolase gene 失效
标题翻译：氯醇和羟基羧酸酯不对称水解酶基因
公开(公告)号：US07335502B2
公开(公告)日：2008-02-26
申请号：US11490749
申请日：2006-07-20
申请人： Atsushi Nakagawa , Toshio Suzuki , Atsuhiko Shinmyo , Ko Kato , Hideaki Idogaki
发明人： Atsushi Nakagawa , Toshio Suzuki , Atsuhiko Shinmyo , Ko Kato , Hideaki Idogaki
IPC分类号： C12N9/18 , C12N5/00 , C12N1/20 , C12P21/06 , C07H21/04
CPC分类号： C12P41/005 , C07K2319/02 , C12N9/18 , C12P7/42 , C12P7/62 , C12P17/04
摘要： The present invention is to provide a gene having asymmetric hydrolase activity which is useful for synthesis of an optically active carboxylic acid, its antipode ester, and lactone, and a hydroxycarboxylic ester asymmetric hydrolase enzyme (EnHCH) derived from Enterobacter sp. DS-S-75 strain (FERM BP-5494) which is bacteria belonging to the genus Enterobacter, a EnHCH gene shown by base sequence of SEQ. ID. NO: 1, a gene encoding a protein having an amino acid sequence of SEQ. ID. NO: 2, and E. coli DH5α (pKK-EnHCH) deposited to International Patent Organism Depositary, National Institute of Advanced Industrial Science and Technology as a deposition No. FERM BP-08466.
摘要翻译：本发明提供具有不对称水解酶活性的基因，其可用于合成光学活性羧酸，其对映体酯和内酯，以及衍生自肠杆菌属的羟基羧酸酯不对称水解酶（EnHCH）。属于肠杆菌属的细菌DS-S-75菌株（FERM BP-5494），由SEQ ID NO：1的碱基序列显示的EnHCH基因。 ID。 NO：1，编码具有SEQ ID NO：1的氨基酸序列的蛋白质的基因。 ID。 NO：2和大肠杆菌DH5α（pKK-EnHCH），保藏在国际专利生物保藏中心，国立先进工业科学技术研究所作为FERM BP-08466。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式