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1. 发明申请

US20110091965A1 CELL MANIPULATION OBSERVATION APPARATUS 审中-公开
标题翻译：细胞操作观察装置
公开(公告)号：US20110091965A1
公开(公告)日：2011-04-21
申请号：US12979070
申请日：2010-12-27
申请人： Kiyohiko Tateyama
发明人： Kiyohiko Tateyama
IPC分类号： C12M1/34
CPC分类号： G06T1/0014
摘要： An introducing position arrangement unit arranges a position of a cell into which a substance is introduced, of the cells in a culture dish mounted on a stage, specified by a specification unit to a predetermined cell manipulation position in a viewing field of an observation unit by driving the stage. A cell manipulation unit introduces an introduction substance mixed in a culture fluid in the culture dish into the cell by making a hole on a cell membrane of the cell moved to the predetermined cell manipulation position by the introducing position arrangement unit. A display unit displays introduced cell information including positional information of the cell indicative of the specified position and introduction substance information indicative of the introduction substance.
摘要翻译：引入位置排列单元将由规格单元指定的台架上安装的培养皿中的单元格放置在观察单元的视野内的预定单元操作位置，将单元格的单元的位置布置在观察单元的视场中开车舞台细胞处理单元通过在导入位置配置单元上移动到规定的细胞操作位置的细胞的细胞膜上形成孔，将培养皿中培养液中混入的导入物质引入细胞。显示单元显示包括指示指定位置的单元的位置信息和表示导入物质的引入物质信息的介质单元信息。

2. 发明申请

US20110091964A1 CELL MANIPULATION OBSERVATION APPARATUS 审中-公开
标题翻译：细胞操作观察装置
公开(公告)号：US20110091964A1
公开(公告)日：2011-04-21
申请号：US12977232
申请日：2010-12-23
申请人： Kiyohiko TATEYAMA
发明人： Kiyohiko TATEYAMA
IPC分类号： C12M1/34 , C12M1/00
CPC分类号： G06T1/0014
摘要： An introducing position arrangement unit arranges a position of a cell into which a substance is introduced, of the cells in a culture dish mounted on a stage, specified by a specification unit to a predetermined cell manipulation position in a viewing field of an observation unit by stage driving. A cell manipulation unit introduces an introduction substance mixed in a culture fluid in the culture dish into the cell by making a hole on a cell membrane of the cell moved to the predetermined cell manipulation position. A diagram display unit displays a diagram associated with an observation range which is a range of the culture dish observable by the observation unit and also displays a cell manipulation range which is a cell manipulation enabled range and a position currently observed by the observation unit to be superimposed on the diagram associated with the observation range.
摘要翻译：引入位置排列单元将由规格单元指定的台架上安装的培养皿中的单元格放置在观察单元的视野内的预定单元操作位置，将单元格的单元的位置布置在观察单元的视场中舞台驾驶细胞操作单元通过在移动到预定细胞操作位置的细胞膜的细胞膜上形成孔，将混合在培养皿中的培养液中的引入物质引入细胞。图表显示单元显示与观察范围相关联的图，该观察范围是由观察单元可观察到的培养皿的范围，并且还显示作为细胞操作使能范围的细胞操作范围和观察单元目前观察到的位置为叠加在与观察范围相关的图上。

3. 发明申请

US20100248342A1 TIP DRIVE APPARATUS AND CANTILEVER TIP 审中-公开
标题翻译：提示驱动器和CANTILEVER提示
公开(公告)号：US20100248342A1
公开(公告)日：2010-09-30
申请号：US12813077
申请日：2010-06-10
申请人： Kiyohiko TATEYAMA , Yasuo Sasaki , Yuka Imaoka
发明人： Kiyohiko TATEYAMA , Yasuo Sasaki , Yuka Imaoka
IPC分类号： C12M1/00
CPC分类号： C12M35/04 , B01L9/54 , B01L2300/0838 , G02B21/32
摘要： A tip drive apparatus is capable to moving a tip unit toward an object, while holding the tip unit at a prescribed angle. The tip unit is formed on a support unit having flexibility and directed to the object at the prescribed angle. The tip unit includes a contact side in a cross section including the distal end of the support unit and extending in the direction the support unit extends, the contact side configured to contact, at one part at least, the object, and to apply a prescribed pressure to the object. The tip unit further includes a first region including the contact side, and a second region continuous to the support unit.
摘要翻译：尖端驱动装置能够将尖端单元朝向物体移动，同时将尖端单元保持在规定的角度。尖端单元形成在具有柔性并且以规定角度指向物体的支撑单元上。尖端单元包括在包括支撑单元的远端并且在支撑单元延伸的方向上延伸的横截面中的接触侧，接触侧构造成在至少一个物体处接触物体并且施加规定的对物体的压力。尖端单元还包括包括接触侧的第一区域和与支撑单元连续的第二区域。

4. 发明申请

US20100248340A1 NEEDLE 审中-公开
标题翻译：针
公开(公告)号：US20100248340A1
公开(公告)日：2010-09-30
申请号：US12797997
申请日：2010-06-10
申请人： Kiyohiko TATEYAMA , Yasuo Sasaki , Yuka Imaoka
发明人： Kiyohiko TATEYAMA , Yasuo Sasaki , Yuka Imaoka
IPC分类号： C12M1/00
CPC分类号： G02B21/26 , C12M35/04
摘要： A needle designed to be manipulated on a single cell includes a cantilever tip including a support unit and a tip unit formed at a prescribed angle to the support unit, and a shaft connected to the cantilever tip. The needle is attachable, via at least the shaft, to a tip drive apparatus capable of moving the tip unit in a prescribed direction.
摘要翻译：设计成在单个电池单元上操作的针包括悬臂尖端，其包括支撑单元和与支撑单元形成为规定角度的尖端单元，以及连接到悬臂尖端的轴。该针可以通过至少轴连接到能够沿着规定方向移动尖端单元的尖端驱动装置。

5. 发明授权

US08343765B2 Gene injection apparatus and gene injection method 有权
标题翻译：基因注射装置和基因注射法
公开(公告)号：US08343765B2
公开(公告)日：2013-01-01
申请号：US12475023
申请日：2009-05-29
申请人： Kiyohiko Tateyama
发明人： Kiyohiko Tateyama
IPC分类号： C12N15/87 , C12M1/00
CPC分类号： C12M35/00
摘要： A gene injection apparatus for injecting a gene into a cell held on a substrate, includes a needle unit. The needle unit includes a fine needle to be inserted into the cell immersed in a culture medium in which the gene is dispersed, and a flexible support part configured to hold the fine needle, the flexible support part being flexing when the fine needle is pressed onto the substrate. The gene injection apparatus further includes a drive unit configured to push down the flexible support part toward the substrate, further from a position where a tip of the fine needle contacts the substrate.
摘要翻译：用于将基因注入保持在基板上的细胞的基因注射装置包括针单元。针单元包括插入插入其中分散有基因的培养基中的细胞的细针和被配置为保持细针的柔性支撑部，柔性支撑部在细针被按压时弯曲底物。基因注射装置还包括驱动单元，该驱动单元被配置为将柔性支撑部朝向基板向下推动，从而使细针的尖端接触基板的位置。

6. 发明申请

US20100250006A1 TIP DRIVE APPARATUS 审中-公开
标题翻译：提示驱动装置
公开(公告)号：US20100250006A1
公开(公告)日：2010-09-30
申请号：US12797951
申请日：2010-06-10
申请人： Yasuo SASAKI , Yuka IMAOKA , Kiyohiko TATEYAMA
发明人： Yasuo SASAKI , Yuka IMAOKA , Kiyohiko TATEYAMA
IPC分类号： G05B15/00
CPC分类号： G02B21/26 , C12M35/04 , G02B21/0088 , G02B21/32
摘要： A tip drive apparatus is capable to moving a tip unit toward an object, while holding the tip unit at a prescribed angle. The tip unit is formed on a support unit having flexibility and directed to the object at the prescribed angle. The tip drive apparatus includes a main unit configured to move the tip unit toward the object, while holding the tip unit at the prescribed angle, an operation module configured to instruct that the tip unit be moved, and an attachment member extending from the main unit and configured to be secured to a condenser lens of an inverted microscope.
摘要翻译：尖端驱动装置能够将尖端单元朝向物体移动，同时将尖端单元保持在规定的角度。尖端单元形成在具有柔性并且以规定角度指向物体的支撑单元上。尖端驱动装置包括主体，其构造成使尖端单元朝向物体移动，同时将尖端单元保持在规定角度;操作模块，其被配置为指示尖端单元移动;以及附接构件，其从主单元并且被配置为固定到倒置显微镜的聚光透镜。

7. 发明申请

US20100248341A1 TIP DRIVE APPARATUS 审中-公开
标题翻译：提示驱动装置
公开(公告)号：US20100248341A1
公开(公告)日：2010-09-30
申请号：US12813038
申请日：2010-06-10
申请人： Yasuo Sasaki , Yuka Imaoka , Kiyohiko Tateyama
发明人： Yasuo Sasaki , Yuka Imaoka , Kiyohiko Tateyama
IPC分类号： C12M1/00
CPC分类号： G02B21/26 , C12M35/04 , G02B21/0088 , G02B21/32
摘要： A tip drive apparatus includes a shaft to which a support unit on which a tip unit is formed, and a main unit configured to move the shaft, thereby to move the tip unit toward an object while remaining held at a prescribed angle. The shaft is arranged to meet four requirements including a first requirement of being prevented from contacting a condenser lens of an inverted microscope to which the main unit is attached, a second requirement of providing a region in which a distal end of the support unit is recognized, a third requirement of being prevented from contacting a sidewall of a dish for holding the object, and a fourth requirement of providing a region in which the dish moves relative to the tip unit.
摘要翻译：尖端驱动装置包括：轴，其上形成有尖端单元的支撑单元和构造成移动轴的主单元，从而使末端单元朝向物体移动，同时保持保持在规定角度。该轴设置成满足四个要求，包括防止与安装有主单元的倒置显微镜的聚光透镜接触的第一要求，第二要求是提供识别支撑单元的远端的区域防止与用于保持物体的盘的侧壁接触的第三要求，以及提供盘相对于尖端单元移动的区域的第四要求。

8. 发明申请

US20100248339A1 TIP DRIVE APPARATUS 审中-公开
标题翻译：提示驱动装置
公开(公告)号：US20100248339A1
公开(公告)日：2010-09-30
申请号：US12797910
申请日：2010-06-10
申请人： Yuka IMAOKA , Yasuo SASAKI , Kiyohiko TATEYAMA
发明人： Yuka IMAOKA , Yasuo SASAKI , Kiyohiko TATEYAMA
IPC分类号： C12M1/10
CPC分类号： G02B21/26 , C12M35/04
摘要： A tip, drive apparatus is capable to moving a tip unit toward an object, while holding the tip unit at a prescribed angle. The tip unit is formed on a support unit having flexibility and directed to the object at the prescribed angle. The tip drive apparatus includes a needle having a shaft to which the support unit on which the tip unit is formed, and an adaptor configured to hold the needle, enabling the needle to be replaced by another. The adaptor is configured to be able to change a positional relation with respect to the main unit of the tip drive apparatus, while the needle is being attached.
摘要翻译：尖端驱动装置能够将尖端单元朝向物体移动，同时将尖端单元保持在规定角度。尖端单元形成在具有柔性并且以规定角度指向物体的支撑单元上。尖端驱动装置包括具有轴的针，其上形成有尖端单元的支撑单元和被配置为保持针的适配器，使得针能够被另一个代替。适配器构造成能够在针被安装时相对于尖端驱动装置的主单元改变位置关系。

9. 发明申请

US20090291502A1 GENE INJECTION APPARATUS AND GENE INJECTION METHOD 有权
标题翻译：基因注射装置和基因注射方法
公开(公告)号：US20090291502A1
公开(公告)日：2009-11-26
申请号：US12475023
申请日：2009-05-29
申请人： Kiyohiko TATEYAMA
发明人： Kiyohiko TATEYAMA
IPC分类号： C12N15/87 , C12M1/00
CPC分类号： C12M35/00
摘要： A gene injection apparatus for injecting a gene into a cell held on a substrate, includes a needle unit. The needle unit includes a fine needle to be inserted into the cell immersed in a culture medium in which the gene is dispersed, and a flexible support part configured to hold the fine needle, the flexible support part being flexing when the fine needle is pressed onto the substrate. The gene injection apparatus further includes a drive unit configured to push down the flexible support part toward the substrate, further from a position where a tip of the fine needle contacts the substrate.
摘要翻译：用于将基因注入保持在基板上的细胞的基因注射装置包括针单元。针单元包括插入插入其中分散有基因的培养基中的细胞的细针和被配置为保持细针的柔性支撑部，柔性支撑部在细针被按压时弯曲底物。基因注射装置还包括驱动单元，该驱动单元被配置为将柔性支撑部朝向基板向下推动，从而使细针的尖端接触基板的位置。

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