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1. 发明授权

US07273749B1 Container for carrying out and monitoring biological processes 有权
标题翻译：用于实施和监测生物过程的容器
公开(公告)号：US07273749B1
公开(公告)日：2007-09-25
申请号：US09631339
申请日：2000-08-03
申请人： Carl T. Wittwer , Kirk M. Ririe
发明人： Carl T. Wittwer , Kirk M. Ririe
IPC分类号： C12M1/34
CPC分类号： G01N21/6452 , B01L3/5082 , B01L7/52 , B01L2300/0838 , B01L2300/1844 , C12Q1/6818 , C12Q1/6823 , C12Q1/686 , G01N21/0303 , G01N21/07 , G01N21/6428 , G01N30/02 , G01N30/06 , G01N30/466 , G01N35/025 , G01N2021/6482 , G01N2035/00237 , Y10S435/808 , Y10S435/809 , C12Q2561/113 , C12Q2531/113 , C12Q2527/107 , C12Q2565/101 , G01N27/447 , B01D15/34
摘要： A thermal cycling method and device is disclosed. The device comprises a sample chamber whose temperature can be rapidly and accurately modulated over a range of temperatures needed to carry out a number of biological procedures, such as the DNA polymerase chain reaction. Biological samples are placed in containers each comprising a reservoir and a reaction portion, wherein the reaction portion has a small volume. The small volume reaction portion permits the rapid and accurate temperature modulation. With an optically transmissible reaction portion, DNA amplification may be monitored by fluorescence during PCR.
摘要翻译：公开了一种热循环方法和装置。该装置包括样品室，其温度可以在进行许多生物学过程（例如DNA聚合酶链式反应）所需的温度范围内快速和准确地调节。将生物样品放置在容器中，每个容器包括储存器和反应部分，其中反应部分具有小的体积。小体积反应部分允许快速和准确的温度调制。通过光学透射的反应部分，可以通过PCR期间的荧光监测DNA扩增。

2. 发明授权

US06232079B1 PCR method for nucleic acid quantification utilizing second or third order rate constants 有权
标题翻译：利用二阶或三阶速率常数进行核酸定量的PCR方法
公开(公告)号：US06232079B1
公开(公告)日：2001-05-15
申请号：US09635344
申请日：2000-08-09
申请人： Carl T. Wittwer , Kirk M. Ririe , Randy P. Rasmussen
发明人： Carl T. Wittwer , Kirk M. Ririe , Randy P. Rasmussen
IPC分类号： C12Q168
CPC分类号： C12Q1/686 , B01L3/5082 , B01L7/52 , B01L2300/0654 , B01L2300/0838 , B01L2300/1844 , C12Q1/6818 , C12Q1/6823 , G01N21/0303 , G01N21/07 , G01N21/253 , G01N21/6428 , G01N21/6452 , G01N35/025 , G01N2021/6417 , G01N2021/6482 , G01N2035/00237 , Y10T436/143333 , C12Q2561/113 , C12Q2531/113 , C12Q2527/107 , C12Q2565/525 , C12Q2563/107 , C12Q2565/101
摘要： The present invention is directed to a method of determining the concentration of a nucleic acid product that had been amplified through polymerase chain reaction (PCR). More particularly, the present invention relates to a method wherein a rate constant is determined for a known concentration of amplified product by monitoring the rate of hybridization of the known concentration, and then the concentration of an unknown concentration of a nucleic acid product can be determined by determining the rate of annealing for the unknown concentration, and calculating the concentration from the rate of annealing and the rate constant.
摘要翻译：本发明涉及通过聚合酶链式反应（PCR）扩增的核酸产物的浓度测定方法。更具体地，本发明涉及一种方法，其中通过监测已知浓度的杂交速率确定已知浓度的扩增产物的速率常数，然后可以确定核酸产物的未知浓度的浓度通过确定未知浓度的退火速率，并从退火速率和速率常数计算浓度。

3. 发明授权

US08394608B2 Self-contained biological analysis 有权
标题翻译：独立生物分析
公开(公告)号：US08394608B2
公开(公告)日：2013-03-12
申请号：US11913120
申请日：2006-05-08
申请人： Kirk M. Ririe , Michael R. Newswander , Randy P. Rasmussen , Mark Aaron Poritz , Stewart Benjamin Smith , David E. Jones , Gary Clark Kessler
发明人： Kirk M. Ririe , Michael R. Newswander , Randy P. Rasmussen , Mark Aaron Poritz , Stewart Benjamin Smith , David E. Jones , Gary Clark Kessler
IPC分类号： C12P19/34
CPC分类号： B01L7/52 , B01L3/502723 , B01L3/50273 , B01L2200/10 , B01L2300/0816 , B01L2300/0867 , B01L2300/087 , B01L2300/123 , B01L2300/1822 , B01L2400/0478 , B01L2400/0481 , B01L2400/049 , B01L2400/0644 , B01L2400/065 , B01L2400/0655 , B01L2400/0677 , B01L2400/0683 , C12Q1/6844
摘要： Devices, containers, and methods are provided for performing biological analysis in a closed environment. Illustrative biological analysis include nucleic acid amplification and detection and immuno-PCR.
摘要翻译：提供设备，容器和方法用于在封闭环境中进行生物分析。说明性的生物学分析包括核酸扩增和检测和免疫PCR。

4. 发明申请

US20090311673A1 NUCLEIC ACID AMPLIFICATION METHODS 审中-公开
标题翻译：核酸扩增方法
公开(公告)号：US20090311673A1
公开(公告)日：2009-12-17
申请号：US11931178
申请日：2007-10-31
申请人： Carl T. Wittwer , Kirk M. Ririe , Randy P. Rasmussen
发明人： Carl T. Wittwer , Kirk M. Ririe , Randy P. Rasmussen
IPC分类号： C12Q1/68 , C12M1/34
CPC分类号： C12Q1/686 , B01L3/5082 , B01L7/52 , B01L2300/0654 , B01L2300/0838 , B01L2300/1844 , C12Q1/6818 , C12Q1/6823 , G01N21/0303 , G01N21/07 , G01N21/253 , G01N21/6428 , G01N21/6452 , G01N35/025 , G01N2021/6417 , G01N2021/6482 , G01N2035/00237 , Y10T436/143333 , C12Q2561/113 , C12Q2531/113 , C12Q2527/107 , C12Q2565/525 , C12Q2563/107 , C12Q2565/101
摘要： The present invention is directed to devices for performing PCR and monitoring the reaction of a sample comprising a nucleic acid and a fluorescent dye. Illustrative devices comprise a heat exchange component for heating and cooling the sample, a control device for repeatedly operating the heat exchange component to subject the sample to thermal cycling, an excitation source for optically exciting the sample to cause the sample to fluoresce, a photodetector for detecting temperature-dependent fluorescence levels from the sample, and a processor configured to record and process emissions from the fluorescent dye.
摘要翻译：本发明涉及用于进行PCR和监测包含核酸和荧光染料的样品的反应的装置。示例性装置包括用于加热和冷却样品的热交换部件，用于重复操作热交换部件以使样品经受热循环的控制装置，用于光学激发样品以引起样品发荧光的激发源，检测来自样品的温度依赖性荧光水平，以及被配置为记录和处理来自荧光染料的发射的处理器。

5. 发明授权

US07081226B1 System and method for fluorescence monitoring 失效
标题翻译：荧光监测系统和方法
公开(公告)号：US07081226B1
公开(公告)日：2006-07-25
申请号：US08869275
申请日：1997-06-04
申请人： Carl T. Wittwer , Kirk M. Ririe , Randy P. Rasmussen , David R. Hillyard
发明人： Carl T. Wittwer , Kirk M. Ririe , Randy P. Rasmussen , David R. Hillyard
IPC分类号： G01N33/50 , C12P19/34
CPC分类号： B01L7/52 , B01L3/5025 , B01L2300/0803 , B01L2300/0838 , B01L2300/1844 , B01L2400/0406 , B01L2400/0409
摘要： A thermal cycling method and device is disclosed. The device comprises a sample chamber whose temperature can be rapidly and accurately modulated over a range of temperatures needed to carry out a number of biological procedures, such as the DNA polymerase chain reaction. Biological samples are placed in glass micro capillary tubes and then located inside the sample chamber. A programmable controller regulates the temperature of the sample inside the sample chamber. Monitoring of the DNA amplification is monitored by fluorescence once per cycle or many times per cycle. The present invention provides that fluorescence monitoring of PCR is a powerful tool for DNA quantification.
摘要翻译：公开了一种热循环方法和装置。该装置包括样品室，其温度可以在进行许多生物学过程（例如DNA聚合酶链式反应）所需的温度范围内快速和准确地调制。将生物样品置于玻璃微毛细管中，然后置于样品室内。可编程控制器调节样品室内样品的温度。 DNA扩增的监测通过每周期荧光一次或每次循环多次监测。本发明提供PCR的荧光监测是DNA定量的有力工具。

6. 发明授权

US5455175A Rapid thermal cycling device 失效
标题翻译：快速热循环装置
公开(公告)号：US5455175A
公开(公告)日：1995-10-03
申请号：US179969
申请日：1994-01-10
申请人： Carl T. Wittwer , David R. Hillyard , Kirk M. Ririe
发明人： Carl T. Wittwer , David R. Hillyard , Kirk M. Ririe
IPC分类号： B01L7/00 , C12M1/38 , G01N30/02 , G01N30/06 , G01N30/30 , G01N30/46 , C12M1/34
CPC分类号： B01L7/52 , G01N2030/3084 , Y10S435/809
摘要： A thermal cycling device having a sample chamber whose temperature can be rapidly and accurately modulated over a range of temperatures needed to carry out a number of biological procedures, such a the DNA polymerase chain reaction. Biological samples are placed in glass microcapillary tubes and then located inside the sample chamber. A programmable controller regulates the temperature of the sample inside the sample chamber. Once a heating cycle is completed, the controller opens a door to the chamber for venting hot air out and cool ambient air is moved in. Temperature versus time profiles corresponding to optimum denaturation, annealing and elongation temperatures for amplification of DNA are achievable by the present invention.
摘要翻译：具有样品室的热循环装置，其温度可以在进行许多生物学过程（例如DNA聚合酶链式反应）所需的温度范围内快速和准确地调制。将生物样品置于玻璃微毛细管中，然后置于样品室内。可编程控制器调节样品室内样品的温度。一旦加热循环完成，控制器打开一个通向室的门，用于排出热空气并冷却环境空气进入。对应于扩增DNA的最佳变性，退火和伸长温度的温度对时间曲线可由本发明实现发明。

7. 发明授权

US09586208B2 Thermal cycling system and method of use 有权
标题翻译：热循环系统及使用方法
公开(公告)号：US09586208B2
公开(公告)日：2017-03-07
申请号：US13936137
申请日：2013-07-05
申请人： Kirk M. Ririe
发明人： Kirk M. Ririe
IPC分类号： C12M1/34 , B01L7/00 , C12Q1/68 , B01L3/00
CPC分类号： B01L7/5255 , B01L3/502 , B01L3/505 , B01L7/52 , B01L7/525 , B01L2300/0654 , B01L2300/1822 , B01L2300/1827 , B01L2400/0481 , C12Q1/686
摘要： A temperature cycling system (10, 110) is provided for repeatedly heating and cooling a reaction mixture (16). The system (10, 110) includes a first heater (27) and a second heater (28) each movable between a first orientation in which the first or second heater (27, 28) affects the temperature of the reaction mixture (16) and a second orientation in which the first or second heater (27, 28) does not substantially affect the temperature of the reaction mixture (16). During temperature cycling, the second heater (28) is in the second orientation when the first heater (27) is in the first orientation, and the second heater (28) is in the first orientation when the first heater (27) is in the second orientation.
摘要翻译：提供温度循环系统（10,110）以反应加热和冷却反应混合物（16）。系统（10,110）包括第一加热器（27）和第二加热器（28），每个第一加热器（27）和第二加热器（28）可在第一方向和第二加热器之间移动，第一方向与第一加热器（27,28）之间影响反应混合物（16）第二取向，其中第一或第二加热器（27,28）基本上不影响反应混合物（16）的温度。在温度循环期间，当第一加热器（27）处于第一方向时，第二加热器（28）处于第二方向，并且当第一加热器（27）处于第一方向时，第二加热器（28）处于第一方向第二个方向。

8. 发明授权

US08343754B2 Annealing curve analysis in PCR 有权
标题翻译： PCR中退火曲线分析
公开(公告)号：US08343754B2
公开(公告)日：2013-01-01
申请号：US13465364
申请日：2012-05-07
申请人： Carl T. Wittwer , Kirk M. Ririe , Randy P. Rasmussen
发明人： Carl T. Wittwer , Kirk M. Ririe , Randy P. Rasmussen
IPC分类号： C12M1/00
CPC分类号： C12Q1/686 , B01L3/5082 , B01L7/52 , B01L2300/0654 , B01L2300/0838 , B01L2300/1844 , C12Q1/6818 , C12Q1/6823 , G01N21/0303 , G01N21/07 , G01N21/253 , G01N21/6428 , G01N21/6452 , G01N35/025 , G01N2021/6417 , G01N2021/6482 , G01N2035/00237 , Y10T436/143333 , C12Q2561/113 , C12Q2531/113 , C12Q2527/107 , C12Q2565/525 , C12Q2563/107 , C12Q2565/101
摘要： The present invention is directed to devices for performing PCR and monitoring the reaction of a sample comprising a nucleic acid and a fluorescent dye. Illustrative devices comprise a heat exchange component for heating and cooling the sample, a control device for repeatedly operating the heat exchange component to subject the sample to thermal cycling, an excitation source for optically exciting the sample to cause the sample to fluoresce, a photodetector for detecting temperature-dependent fluorescence levels from the sample, and a processor configured to record and process emissions from the fluorescent dye.
摘要翻译：本发明涉及用于进行PCR和监测包含核酸和荧光染料的样品的反应的装置。示例性装置包括用于加热和冷却样品的热交换部件，用于重复操作热交换部件以使样品经受热循环的控制装置，用于光学激发样品以引起样品发荧光的激发源，检测来自样品的温度依赖性荧光水平，以及被配置为记录和处理来自荧光染料的发射的处理器。

9. 发明授权

US07745205B2 Container for carrying out and monitoring biological processes 有权
标题翻译：用于实施和监测生物过程的容器
公开(公告)号：US07745205B2
公开(公告)日：2010-06-29
申请号：US10914648
申请日：2004-08-09
申请人： Carl T. Wittwer , Kirk M. Ririe
发明人： Carl T. Wittwer , Kirk M. Ririe
IPC分类号： C12M1/34
CPC分类号： G01N21/6452 , B01L3/5082 , B01L7/52 , B01L2300/0838 , B01L2300/1844 , C12Q1/6818 , C12Q1/6823 , C12Q1/686 , G01N21/0303 , G01N21/07 , G01N21/6428 , G01N30/02 , G01N30/06 , G01N30/466 , G01N35/025 , G01N2021/6482 , G01N2035/00237 , Y10S435/808 , Y10S435/809 , C12Q2561/113 , C12Q2531/113 , C12Q2527/107 , C12Q2565/101 , G01N27/447 , B01D15/34
摘要： A thermal cycling method and device is disclosed. The device comprises a sample chamber whose temperature can be rapidly and accurately modulated over a range of temperatures needed to carry out a number of biological procedures, such as the DNA polymerase chain reaction. Biological samples are placed in containers each comprising a reservoir and a reaction portion, wherein the reaction portion has a small volume. The small volume reaction portion permits the rapid and accurate temperature modulation. With an optically transmissible reaction portion, DNA amplification may be monitored by fluorescence during PCR.
摘要翻译：公开了一种热循环方法和装置。该装置包括样品室，其温度可以在进行许多生物学过程（例如DNA聚合酶链式反应）所需的温度范围内快速和准确地调制。将生物样品放置在容器中，每个容器包括储存器和反应部分，其中反应部分具有小的体积。小体积反应部分允许快速和准确的温度调制。通过光学透射的反应部分，可以通过PCR期间的荧光监测DNA扩增。

10. 发明申请

US20090258414A1 SYSTEM FOR FLUORESCENCE MONITORING 审中-公开
标题翻译：荧光监测系统
公开(公告)号：US20090258414A1
公开(公告)日：2009-10-15
申请号：US11926775
申请日：2007-10-29
申请人： Carl T. Wittwer , Kirk M. Ririe , Randy P. Rasmussen
发明人： Carl T. Wittwer , Kirk M. Ririe , Randy P. Rasmussen
IPC分类号： C12M1/00
CPC分类号： C12Q1/686 , B01L3/5082 , B01L7/52 , B01L2300/0654 , B01L2300/0838 , B01L2300/1844 , C12Q1/6818 , C12Q1/6823 , G01N21/0303 , G01N21/07 , G01N21/253 , G01N21/6428 , G01N21/6452 , G01N35/025 , G01N2021/6417 , G01N2021/6482 , G01N2035/00237 , Y10T436/143333 , C12Q2561/113 , C12Q2531/113 , C12Q2527/107 , C12Q2565/525 , C12Q2563/107 , C12Q2565/101
摘要： The present invention is directed to devices for performing PCR and monitoring the reaction of a sample comprising a nucleic acid and a fluorescent dye. Illustrative devices comprise a heat exchange component for heating and cooling the sample, a control device for repeatedly operating the heat exchange component to subject the sample to thermal cycling, an excitation source for optically exciting the sample to cause the sample to fluoresce, a photodetector for detecting temperature-dependent fluorescence levels from the sample, and a processor configured to record and process emissions from the fluorescent dye.
摘要翻译：本发明涉及用于进行PCR和监测包含核酸和荧光染料的样品的反应的装置。示例性装置包括用于加热和冷却样品的热交换部件，用于重复操作热交换部件以使样品经受热循环的控制装置，用于光学激发样品以引起样品发荧光的激发源，检测来自样品的温度依赖性荧光水平，以及被配置为记录和处理来自荧光染料的发射的处理器。

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