专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

US20120034653A1 Trophic Conversion of Obligate Phototrophic Algae Through Metabolic Engineering 审中-公开
标题翻译：通过代谢工程营养转化特种光营养藻类
公开(公告)号：US20120034653A1
公开(公告)日：2012-02-09
申请号：US13188806
申请日：2011-07-22
申请人： Kirk Emil APT , F.C. Thomas Allnutt , David J. Kyle , James Casey Lippmeier
发明人： Kirk Emil APT , F.C. Thomas Allnutt , David J. Kyle , James Casey Lippmeier
IPC分类号： C12P21/00 , C12N15/79 , C12N1/13 , C07C29/74 , C11B1/10 , C07C35/21 , C07C7/00 , C07K14/405 , A23K1/00 , C07K1/14
CPC分类号： C12N15/8209 , C12N1/12 , C12N15/65 , C12N15/79 , C12N15/8207 , Y10S435/946
摘要： Most microalgae are obligate photoautotrophs and their growth is strictly dependent on the generation of photosynthetically-derived energy. In this study it is shown that the microalga Phaeodaclylurn tricornutum can be engineered to import glucose and grow in the dark through the introduction of genes encoding glucose transporters. Both the human and Chlorella kessleri glucose transporters facilitated the uptake of glucose by P. tricornutum, allowing the cells to metabolize exogenous organic carbon and thrive, independent of light. This is the first successful trophic conversion of an obligate photoautotroph through metabolic engineering, and it demonstrates that methods of cell nourishment can be fundamentally altered with the introduction of a single gene. Since strains transformed with the glucose transport genes are able to grow non-photosynthetically, they can be exploited for the analysis of photosynthetic processes through mutant generation and characterization. Finally, this work also represents critical progress toward large-scale commercial exploitation of obligate phototrophic algae through the use of microbial fermentation technology, eliminating significant limitations resulting from light-dependent growth.
摘要翻译：大多数微藻是专性光自养体，其生长严格依赖于光合作用衍生能量的产生。在这项研究中，显示微藻Phaeodaclylurn三角褐豆可以通过引入编码葡萄糖转运蛋白的基因进行工程化以导入葡萄糖并在黑暗中生长。人和小球藻凯斯勒葡萄糖转运蛋白都促进了三角褐指藻的葡萄糖摄取，使得细胞代谢外源有机碳，并且独立于光。这是通过代谢工程首次成功地营养专一性自养型营养转化，并且表明通过引入单一基因可以从根本上改变细胞营养的方法。由于用葡萄糖转运基因转化的菌株能够非光合作用生长，因此可以通过突变体的产生和表征来开发光合作用的分析。最后，这项工作也是通过使用微生物发酵技术大规模商业开发专用光营养藻类的重要进展，消除了光依赖性生长造成的重大限制。

2. 发明授权

US09012197B2 Production of hemagglutinin-neuraminidase protein in microalgae 有权
标题翻译：在微藻中生产血凝素 - 神经氨酸酶蛋白
公开(公告)号：US09012197B2
公开(公告)日：2015-04-21
申请号：US12980320
申请日：2010-12-28
申请人： Anne-Cécile V. Bayne , James Casey Lippmeier , Kirk Emil Apt , Xuan Guo , Joyce A. Pritchard
发明人： Anne-Cécile V. Bayne , James Casey Lippmeier , Kirk Emil Apt , Xuan Guo , Joyce A. Pritchard
IPC分类号： C12N9/26 , C12N9/14 , C12N1/12 , C12P21/06 , C07H21/04 , C07K1/00 , C12N7/00 , C12N15/82 , C12N9/24
CPC分类号： C12Y302/01018 , C12N7/00 , C12N9/2402 , C12N15/8257 , C12N2760/18151 , C12N2760/18651
摘要： The present invention is directed to recombinant microalgal cells and their use in production of heterologous hemagglutinin-neuraminidase (HN) polypeptides, as well as compositions and uses thereof.
摘要翻译：本发明涉及重组微藻细胞及其在产生异源血凝素 - 神经氨酸酶（HN）多肽中的用途，以及其组合物和用途。

3. 发明申请

US20110189228A1 Production of Heterologous Polypeptides in Microalgae, Microalgal Extracellular Bodies, Compositions, and Methods of Making and Uses Thereof 审中-公开
标题翻译：在微藻，微藻胞外体，组合物及其制备和使用方法中产生异源多肽
公开(公告)号：US20110189228A1
公开(公告)日：2011-08-04
申请号：US12980319
申请日：2010-12-28
申请人： Anne-Cécíle V. BAYNE , James Casey LIPPMEIER , Kirk Emil APT , Ross Eric ZIRKLE
发明人： Anne-Cécíle V. BAYNE , James Casey LIPPMEIER , Kirk Emil APT , Ross Eric ZIRKLE
IPC分类号： A61K39/12 , C12P21/00 , C12N9/24 , C12N1/13 , C07K14/005 , A61P31/12 , A61P37/04
CPC分类号： C12P21/02 , A61K39/145 , A61K2039/5258 , C07K14/005 , C12N1/12 , C12N7/00 , C12N9/2402 , C12N2760/16123 , C12N2760/16134 , C12N2760/16152 , C12Y302/01018
摘要： The present invention relates to recombinant microalgal cells and their use in heterologous protein production, methods of production of heterologous polypeptides in microalgal extracellular bodies, microalgal extracellular bodies comprising heterologous polypeptides, and compositions comprising the same.
摘要翻译：本发明涉及重组微藻细胞及其在异源蛋白质生产中的应用，在微藻胞外体中产生异源多肽的方法，包含异源多肽的微藻胞外体以及包含其的组合物。

4. 发明授权

US07939710B1 Trophic conversion of obligate phototrophic algae through metabolic engineering 失效
公开(公告)号：US07939710B1
公开(公告)日：2011-05-10
申请号：US09839536
申请日：2001-04-23
申请人： Kirk Emil Apt , F. C. Thomas Allnutt , David J. Kyle , James Casey Lippmeier
发明人： Kirk Emil Apt , F. C. Thomas Allnutt , David J. Kyle , James Casey Lippmeier
IPC分类号： C12N15/82
CPC分类号： C12N15/8209 , C12N1/12 , C12N15/65 , C12N15/79 , C12N15/8207 , Y10S435/946
摘要： Most microalgae are obligate photoautotrophs and their growth is strictly dependent on the generation of photosynthetically-derived energy. In this study it is shown that the microalga Phaeodaclylurn tricornutum can be engineered to import glucose and grow in the dark through the introduction of genes encoding glucose transporters. Both the human and Chlorella kessleri glucose transporters facilitated the uptake of glucose by P. tricornutum, allowing the cells to metabolize exogenous organic carbon and thrive, independent of light. This is the first successful trophic conversion of an obligate photoautotroph through metabolic engineering, and it demonstrates that methods of cell nourishment can be fundamentally altered with the introduction of a single gene. Since strains transformed with the glucose transport genes are able to grow non-photosynthetically, they can be exploited for the analysis of photosynthetic processes through mutant generation and characterization. Finally, this work also represents critical progress toward large-scale commercial exploitation of obligate phototrophic algae through the use of microbial fermentation technology, eliminating significant limitations resulting from light-dependent growth.

5. 发明申请

US20080138851A1 TROPHIC CONVERSION OF OBLIGATE PHOTOTROPHIC ALGAE THROUGH METABOLIC ENGINEERING 失效
标题翻译：通过代谢工程进行的白藜芦醇的热转化
公开(公告)号：US20080138851A1
公开(公告)日：2008-06-12
申请号：US11842898
申请日：2007-08-21
申请人： Kirk Emil Apt , F.C. Thomas Allnutt , David J. Kyle , James Casey Lippmeier
发明人： Kirk Emil Apt , F.C. Thomas Allnutt , David J. Kyle , James Casey Lippmeier
IPC分类号： C12Q1/02 , C12N1/12 , C12N15/74
CPC分类号： C12N15/8209 , C12N1/12 , C12N15/65 , C12N15/79 , C12N15/8207 , Y10S435/946
摘要： Most microalgae are obligate photoautotrophs and their growth is strictly dependent on the generation of photosynthetically-derived energy. In this study it is shown that the microalga Phaeodaclylurn tricornutum can be engineered to import glucose and grow in the dark through the introduction of genes encoding glucose transporters. Both the human and Chlorella kessleri glucose transporters facilitated the uptake of glucose by P. tricornutum, allowing the cells to metabolize exogenous organic carbon and thrive, independent of light. This is the first successful trophic conversion of an obligate photoautotroph through metabolic engineering, and it demonstrates that methods of cell nourishment can be fundamentally altered with the introduction of a single gene. Since strains transformed with the glucose transport genes are able to grow non-photosynthetically, they can be exploited for the analysis of photosynthetic processes through mutant generation and characterization. Finally, this work also represents critical progress toward large-scale commercial exploitation of obligate phototrophic algae through the use of microbial fermentation technology, eliminating significant limitations resulting from light-dependent growth.
摘要翻译：大多数微藻是专性光自养体，其生长严格依赖于光合作用衍生能量的产生。在这项研究中，显示微藻Phaeodaclylurn三角褐豆可以通过引入编码葡萄糖转运蛋白的基因进行工程化以导入葡萄糖并在黑暗中生长。人和小球藻凯斯勒葡萄糖转运蛋白都促进了三角褐指藻的葡萄糖摄取，使得细胞代谢外源有机碳，并且独立于光。这是通过代谢工程首次成功地营养专一性自养型营养转化，并且表明通过引入单一基因可以从根本上改变细胞营养的方法。由于用葡萄糖转运基因转化的菌株能够非光合作用生长，因此可以通过突变体的产生和表征来开发光合作用的分析。最后，这项工作也是通过使用微生物发酵技术大规模商业开发专用光营养藻类的重要进展，消除了光依赖性生长造成的重大限制。

6. 发明申请

US20150110826A1 Production of Heterologous Polypeptides in Microalgae, Microalgal Extracellular Bodies, Compositions, and Methods of Making and Uses Thereof 审中-公开
标题翻译：在微藻，微藻胞外体，组合物及其制备和使用方法中产生异源多肽
公开(公告)号：US20150110826A1
公开(公告)日：2015-04-23
申请号：US14516634
申请日：2014-10-17
申请人： Anne-Cecile V. BAYNE , James Casey LIPPMEIER , Kirk Emil APT , Ross Eric ZIRKLE
发明人： Anne-Cecile V. BAYNE , James Casey LIPPMEIER , Kirk Emil APT , Ross Eric ZIRKLE
IPC分类号： C12P21/02 , C12N7/00 , C07K14/005 , A61K39/145 , C12N9/24
CPC分类号： C12P21/02 , A61K39/145 , A61K2039/5258 , C07K14/005 , C12N1/12 , C12N7/00 , C12N9/2402 , C12N2760/16123 , C12N2760/16134 , C12N2760/16152 , C12Y302/01018
摘要： The present invention relates to recombinant microalgal cells and their use in heterologous protein production, methods of production of heterologous polypeptides in microalgal extracellular bodies, microalgal extracellular bodies comprising heterologous polypeptides, and compositions comprising the same.
摘要翻译：本发明涉及重组微藻细胞及其在异源蛋白质生产中的应用，在微藻胞外体中产生异源多肽的方法，包含异源多肽的微藻胞外体以及包含其的组合物。

7. 发明申请

US20110195480A1 PRODUCTION OF HEMAGGLUTININ-NEURAMINIDASE PROTEIN IN MICROALGAE 有权
标题翻译：在微藻中生产HEMAGGLUTININ-NEURAMINIDASE蛋白
公开(公告)号：US20110195480A1
公开(公告)日：2011-08-11
申请号：US12980320
申请日：2010-12-28
申请人： Anne-Cécile V. Bayne , James Casey Lippmeier , Kirk Emil APT , Xuan Guo , Joyce A. Pritchard
发明人： Anne-Cécile V. Bayne , James Casey Lippmeier , Kirk Emil APT , Xuan Guo , Joyce A. Pritchard
IPC分类号： C12N9/26 , C12N1/13
CPC分类号： C12Y302/01018 , C12N7/00 , C12N9/2402 , C12N15/8257 , C12N2760/18151 , C12N2760/18651
摘要： The present invention is directed to recombinant microalgal cells and their use in production of heterologous hemagglutinin-neuraminidase (HN) polypeptides, as well as compositions and uses thereof.
摘要翻译：本发明涉及重组微藻细胞及其在产生异源血凝素 - 神经氨酸酶（HN）多肽中的用途，以及其组合物和用途。

8. 发明申请

US20200063174A1 Production of Heterologous Polypeptides in Microalgae, Microalgal Extracellular Bodies, Compositions, and Methods of Making and Uses Thereof 审中-公开
公开(公告)号：US20200063174A1
公开(公告)日：2020-02-27
申请号：US16559103
申请日：2019-09-03
申请人： Anne-Cecile V. BAYNE , James Casey LIPPMEIER , Kirk Emil APT , Ross Eric ZIRKLE
发明人： Anne-Cecile V. BAYNE , James Casey LIPPMEIER , Kirk Emil APT , Ross Eric ZIRKLE
IPC分类号： C12P21/02 , A61K39/145 , C07K14/005 , C12N7/00 , C12N9/24 , C12N1/12
摘要： The present invention relates to recombinant microalgal cells and their use in heterologous protein production, methods of production of heterologous polypeptides in microalgal extracellular bodies, microalgal extracellular bodies comprising heterologous polypeptides, and compositions comprising the same.

9. 发明授权

US08008061B2 Trophic conversion of obligate phototrophic algae through metabolic engineering 失效
标题翻译：通过代谢工程营养转化专性光营养藻类
公开(公告)号：US08008061B2
公开(公告)日：2011-08-30
申请号：US11842898
申请日：2007-08-21
申请人： Kirk Emil Apt , F. C. Thomas Allnutt , David J. Kyle , James Casy Lippmeier
发明人： Kirk Emil Apt , F. C. Thomas Allnutt , David J. Kyle , James Casy Lippmeier
IPC分类号： C12N1/12
CPC分类号： C12N15/8209 , C12N1/12 , C12N15/65 , C12N15/79 , C12N15/8207 , Y10S435/946
摘要： Most microalgae are obligate photoautotrophs and their growth is strictly dependent on the generation of photosynthetically-derived energy. In this study it is shown that the microalga Phaeodaclylurn tricornutum can be engineered to import glucose and grow in the dark through the introduction of genes encoding glucose transporters. Both the human and Chlorella kessleri glucose transporters facilitated the uptake of glucose by P. tricornutum, allowing the cells to metabolize exogenous organic carbon and thrive, independent of light. This is the first successful trophic conversion of an obligate photoautotroph through metabolic engineering, and it demonstrates that methods of cell nourishment can be fundamentally altered with the introduction of a single gene. Since strains transformed with the glucose transport genes are able to grow non-photosynthetically, they can be exploited for the analysis of photosynthetic processes through mutant generation and characterization. Finally, this work also represents critical progress toward large-scale commercial exploitation of obligate phototrophic algae through the use of microbial fermentation technology, eliminating significant limitations resulting from light-dependent growth.
摘要翻译：大多数微藻是专性光自养体，其生长严格依赖于光合作用衍生能量的产生。在这项研究中，显示微藻Phaeodaclylurn三角褐豆可以通过引入编码葡萄糖转运蛋白的基因进行工程化以导入葡萄糖并在黑暗中生长。人和小球藻凯斯勒葡萄糖转运蛋白都促进了三角褐指藻的葡萄糖摄取，使得细胞代谢外源有机碳，并且独立于光。这是通过代谢工程首次成功地营养专一性自养型营养转化，并且表明通过引入单一基因可以从根本上改变细胞营养的方法。由于用葡萄糖转运基因转化的菌株能够非光合作用生长，因此可以通过突变体的产生和表征来开发光合作用的分析。最后，这项工作也是通过使用微生物发酵技术大规模商业开发专利性光营养藻类的重要进展，消除了光依赖性生长造成的重大限制。

10. 发明申请

US20110195449A1 Recombinant Thraustochytrids that Grow on Sucrose, and Compositions, Methods of Making, and Uses Thereof 审中-公开
标题翻译：在蔗糖上生长的重组Thraustochytrids及其组合物，制备方法及其用途
公开(公告)号：US20110195449A1
公开(公告)日：2011-08-11
申请号：US12980322
申请日：2010-12-28
申请人： James Casey Lippmeier , Kirk Emil Apt , Jon Milton Hansen
发明人： James Casey Lippmeier , Kirk Emil Apt , Jon Milton Hansen
IPC分类号： C12P1/00 , C12N15/63 , C12N1/13
CPC分类号： C12N15/79 , A23K20/10 , A23K50/80 , A23L2/52 , A23L33/10 , A23V2002/00 , C12N1/10 , C12N1/12 , C12N9/2405 , C12P7/6463 , C12P7/649 , C12Y302/01026 , C12Y302/01048 , Y02E50/13
摘要： The present invention is directed to recombinant thraustochytrids that grow on sucrose and cell cultures comprising the recombinant thraustochytrids as well as methods of producing cell cultures, biomasses, microbial oils, compositions, and biofuels using the recombinant thraustochytrids.
摘要翻译：本发明涉及在蔗糖上生长的重组thraustochytrid和包含重组thraustochytrids的细胞培养物，以及使用重组thraustochytrids生产细胞培养物，生物质，微生物油，组合物和生物燃料的方法。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式