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    • 5. 发明授权
    • Cell culturing method using biomechanical stimulation loading and system therefor
    • 使用生物力学刺激装载的细胞培养方法及其系统
    • US07968335B2
    • 2011-06-28
    • US11629619
    • 2005-06-16
    • Ken Nakata
    • Ken Nakata
    • C12N5/00
    • C12M35/04C12M23/50C12M41/14
    • A cell culturing method employing biomechanical stimulation loading and a system for applying, in in vitro culture, stimulation similar to vertical weight load stimulation or sideward shear stress stimulation acting on tissues in an aboveground living body as repeated biomechanical stimulation to culture cells, culture tissues or a construct including the culture cells. The system includes a mechanical stimulation loading device and a culture receptacle that are placed in a carbon dioxide incubator, and a control computer. The device includes a weight loading piston, a piston raising and lowering stage supporting piston to allow vertical movement of a piston within a designated range, and a stage lifting and lowering mechanism for vertically moving the stage. A computer drives the mechanism in a specified cycle to effect the vertical movement of the stage. When the stage is lowered, a culture in the receptacle is weighted with the piston.
    • 使用生物力学刺激加载的细胞培养方法和用于在体外培养中应用类似于在地上生物体中的组织上作用的垂直重量负荷刺激或侧向剪切应力刺激的刺激的系统,作为对培养细胞,培养组织或重复的生物力学刺激的系统 包括培养细胞的构建体。 该系统包括放置在二氧化碳培养箱中的机械刺激加载装置和培养容器以及控制计算机。 该装置包括重量装载活塞,活塞升降台支撑活塞以允许活塞在指定范围内的垂直运动,以及用于垂直移动平台的台升降机构。 计算机在指定的周期内驱动机构以实现平台的垂直运动。 当舞台下降时,容器中的培养物用活塞加重。