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1. 发明授权

US08859241B2 Process for production of L-amino acid 有权
标题翻译：生产L-氨基酸的方法
公开(公告)号：US08859241B2
公开(公告)日：2014-10-14
申请号：US13520954
申请日：2011-01-07
申请人： Mikiro Hayashi , Kazuhiko Tabata
发明人： Mikiro Hayashi , Kazuhiko Tabata
IPC分类号： C12P13/04 , C12N9/00 , C12N1/20 , C12N15/00 , C07H21/02 , C12P13/14 , C12R1/19
CPC分类号： C12P13/04 , C12P13/14 , C12R1/19 , C12Y301/02012
摘要： Provided is a novel process for producing an L-amino acid using a microorganism belonging to the genus Escherichia. According to the present invention, a process for producing an L-amino acid comprising; culturing a microorganism in which an activity of the protein of any of the following [1]-[3] is increased compared with that of the parent strain in a medium, producing and accumulating the L-amino acid in the medium, and recovering the L-amino acid from the medium: [1] a protein comprising the amino acid sequence shown in SEQ ID NO: 2 [2] a protein consisting of an amino acid sequence in which one or more amino acids are deleted, substituted or added in the amino acid sequence shown in SEQ ID NO: 2, and having YeiG activity [3] a protein consisting of an amino acid sequence having 80% or more homology to the amino acid sequence shown in SEQ ID NO: 2, and having YeiG activity.
摘要翻译：提供了使用属于埃希氏菌属的微生物来生产L-氨基酸的新方法。根据本发明，制备L-氨基酸的方法包括：培养下述[1] - [3]中任一项所述的蛋白质的活性与培养基中的亲本菌株相比增加的微生物，培养基中产生和积累L-氨基酸，并回收来自培养基的L-氨基酸：[1]包含SEQ ID NO：2所示氨基酸序列的蛋白质[2]一种蛋白质，其由一个或多个氨基酸缺失，取代或加入的氨基酸序列组成 SEQ ID NO：2所示的氨基酸序列，并且具有YeiG活性[3]由与SEQ ID NO：2所示的氨基酸序列具有80％以上同源性的氨基酸序列组成的蛋白质，并具有YeiG活性。

2. 发明授权

US08623619B2 Process for producing L-amino acid 有权
标题翻译：生产L-氨基酸的方法
公开(公告)号：US08623619B2
公开(公告)日：2014-01-07
申请号：US13148564
申请日：2010-02-09
申请人： Kazuhiko Tabata , Akihiro Senoo
发明人： Kazuhiko Tabata , Akihiro Senoo
IPC分类号： C12P13/04 , C12P13/22 , C12N9/00 , C12N1/20 , C12N15/00 , C07H21/04
CPC分类号： C12P13/06 , C07K14/195 , C12P13/08 , C12P13/12 , C12P13/14 , C12P13/222
摘要： The present invention provides a method of improving efficiency of a fermentative production of an L-amino acid. To be specific, the present invention provides a process for producing a L-amino acid, comprising culturing a microorganism wherein the activity of the protein described in any one of (1) to (3) below is higher than that of the parent strain in a medium to produce the L-amino acid and accumulate the L-amino acid in the medium, and then collecting the L-amino acid from the medium: (1) a protein comprising the amino acid sequence shown by any one of SEQ ID NOS:2, 4, 6 and 8 (2) a protein consisting of the amino acid sequence resulting from deletion, substitution or addition of one or more amino acids in the amino acid sequence shown in any one of SEQ ID NOS:2, 4, 6 and 8, and having L-amino acid transport activity (3) a protein consisting of the amino acid sequence having 80% or more homology to the amino acid sequence shown in any one of SEQ ID NOS:2, 4, 6 and 8, and having L-amino acid transport activity.
摘要翻译：本发明提供了提高L-氨基酸的发酵生产效率的方法。具体而言，本发明提供了L-氨基酸的制造方法，其特征在于，含有下述（1）〜（3）中任一项所述的蛋白质的活性高于母体菌株的活性的微生物产生L-氨基酸并在培养基中积累L-氨基酸的培养基，然后从培养基中收集L-氨基酸：（1）包含SEQ ID NOS中任一个所示的氨基酸序列的蛋白质 2,4,6和8（2）由SEQ ID NO：2,4,4,7所示的氨基酸序列中的一个或多个氨基酸的缺失，取代或添加得到的氨基酸序列组成的蛋白质， 6和8，并且具有L-氨基酸转运活性（3）由与SEQ ID NO：2,4,6和8中任一个所示的氨基酸序列具有80％或更高同源性的氨基酸序列组成的蛋白质并具有L-氨基酸转运活性。

3. 发明申请

US20090191589A1 PROCESS FOR PRODUCING DIPEPTIDES 有权
标题翻译：生产皂苷的方法
公开(公告)号：US20090191589A1
公开(公告)日：2009-07-30
申请号：US12406330
申请日：2009-03-18
申请人： Shin-ichi Hashimoto , Kazuhiko Tabata , Aya Kubota , Hajime Ikeda
发明人： Shin-ichi Hashimoto , Kazuhiko Tabata , Aya Kubota , Hajime Ikeda
IPC分类号： C12P21/02
CPC分类号： C12P21/02 , C12N9/00 , C12N9/54
摘要： The present invention provides a protein which catalyzes the synthesis of a dipeptide different from L-Ala-L-Ala, a process for producing the protein which catalyzes the synthesis of a dipeptide, a process for producing a dipeptide using the protein which catalyzes the synthesis of a dipeptide, and a process for producing the dipeptide using a culture of a microorganism producing the protein which catalyzes the synthesis of a dipeptide or the like as an enzyme source.
摘要翻译：本发明提供了催化不同于L-Ala-L-Ala的二肽的合成的蛋白质，用于生产催化二肽合成的蛋白质的方法，使用催化合成的蛋白质生产二肽的方法的二肽的方法，以及使用产生作为酶源的二肽等的合成的蛋白质的微生物的培养物来生成二肽的方法。

4. 发明申请

US20080213827A1 Process For Producing Dipeptides or Dipeptide Derivatives 审中-公开
标题翻译：产生二肽或二肽衍生物的方法
公开(公告)号：US20080213827A1
公开(公告)日：2008-09-04
申请号：US11630397
申请日：2005-06-24
申请人： Shin-ichi Hashimoto , Hajime Ikeda , Kazuhiko Tabata
发明人： Shin-ichi Hashimoto , Hajime Ikeda , Kazuhiko Tabata
IPC分类号： C12P21/04 , C07K7/00
CPC分类号： C12P21/02
摘要： The present invention provides a process for producing a dipeptide or a dipeptide derivative by using a protein having the activity to form the dipeptide or dipeptide derivative from one or more kinds of amino acids or amino acid derivatives, or a culture of cells having the ability to produce the protein or a treated matter of the culture as a enzyme source, which comprise;allowing the enzyme source, one or more kinds of amino acids or amino acid derivatives and ATP to be present in an aqueous medium; allowing the dipeptide or dipeptide derivative to form and accumulate in the medium; and recovering the dipeptide or dipeptide derivative from the medium.
摘要翻译：本发明提供了通过使用具有从一种或多种氨基酸或氨基酸衍生物形成二肽或二肽衍生物的活性的蛋白质或具有以下能力的细胞培养物来生产二肽或二肽衍生物的方法：产生蛋白质或培养物的处理物质作为酶源，其包含：允许酶源，一种或多种氨基酸或氨基酸衍生物和ATP存在于水性介质中; 允许二肽或二肽衍生物在培养基中形成和积累; 并从培养基中回收二肽或二肽衍生物。

5. 发明授权

US08211688B2 Process for producing L-glutamine using Escherichia coli with deficient glnB and glnE function 有权
标题翻译：使用具有缺失glnB和glnE功能的大肠杆菌生产L-谷氨酰胺的方法
公开(公告)号：US08211688B2
公开(公告)日：2012-07-03
申请号：US11630396
申请日：2005-06-24
申请人： Shin-ichi Hashimoto , Kazuhiko Tabata
发明人： Shin-ichi Hashimoto , Kazuhiko Tabata
IPC分类号： C12P13/14 , C12N1/20
CPC分类号： C12P13/14 , C12P13/227
摘要： The present invention provides a microorganism belonging to the genus Escherichia in which the activities of glutamine-synthetase adenylyltransferase (GlnE protein) and PII regulatory protein for glutamine synthetase are reduced or lost and which has the ability to form and accumulate L-glutamine or a substance biosynthesized utilizing nitrogen supplied by L-glutamine, and a process for producing L-glutamine or the substance biosynthesized utilizing nitrogen supplied by L-glutamine using the microorganism.
摘要翻译：本发明提供了属于埃希氏杆菌属的微生物，其中谷氨酰胺合成酶腺苷酰转移酶（GlnE蛋白）和谷氨酰胺合成酶的PII调节蛋白的活性降低或丧失，并且具有形成和积累L-谷氨酰胺或物质的能力使用由L-谷氨酰胺提供的氮进行生物合成，以及使用该微生物生产L-谷氨酰胺的方法或利用由L-谷氨酰胺提供的氮生物合成的物质。

6. 发明授权

US07939302B2 Process for producing dipeptides 有权
标题翻译：二肽生产方法
公开(公告)号：US07939302B2
公开(公告)日：2011-05-10
申请号：US12400996
申请日：2009-03-10
申请人： Shin-ichi Hashimoto , Kazuhiko Tabata
发明人： Shin-ichi Hashimoto , Kazuhiko Tabata
IPC分类号： C12P1/00 , C12P13/00 , C12P7/40
CPC分类号： C12N9/93 , C07K5/06026 , C07K5/06034 , C07K5/0606 , C07K5/06069 , C12P21/02
摘要： The present invention provides a process for producing a dipeptide which comprises culturing in a medium a microorganism which has the ability to produce a protein having the activity to form the dipeptide from one or more kinds of amino acids and which has the ability to produce at least one of said one or more kinds of amino acids, allowing the dipeptide to form and accumulate in the medium, and recovering the dipeptide from the medium.
摘要翻译：本发明提供一种生产二肽的方法，其包括在培养基中培养一种微生物，该微生物具有从一种或多种氨基酸产生具有形成二肽的活性的蛋白质的能力，并且其具有至少产生所述一种或多种氨基酸之一，允许二肽在培养基中形成和积累，并从培养基中回收二肽。

7. 发明申请

US20100129867A1 METHOD FOR PRODUCING DIPEPTIDE 有权
标题翻译：生产二肽的方法
公开(公告)号：US20100129867A1
公开(公告)日：2010-05-27
申请号：US12594903
申请日：2008-04-04
申请人： Mikiro Hayashi , Kazuhiko Tabata , Makoto Yagasaki , Yoshiyuki Yonetani
发明人： Mikiro Hayashi , Kazuhiko Tabata , Makoto Yagasaki , Yoshiyuki Yonetani
IPC分类号： C12P21/00 , C12N1/21
CPC分类号： C07K14/195 , C07K5/06026 , C12P21/02
摘要： The invention provides a microorganism having an ability to produce a protein having a dipeptide synthesizing activity and in which an activity of the protein to transport a dipeptide in a microbial cell to the outside of the microbial cell is higher than that of a parental strain.
摘要翻译：本发明提供了具有产生具有二肽合成活性的蛋白质的能力的微生物，其中将微生物细胞中的二肽转运至微生物细胞外的蛋白质的活性高于亲本菌株的活性。

8. 发明授权

US07579175B2 Process for producing N-acetylneuraminic acid 有权
标题翻译： N-乙酰神经氨酸生产方法
公开(公告)号：US07579175B2
公开(公告)日：2009-08-25
申请号：US10338872
申请日：2003-01-09
申请人： Satoshi Koizumi , Kazuhiko Tabata , Tetsuo Endo , Akio Ozaki
发明人： Satoshi Koizumi , Kazuhiko Tabata , Tetsuo Endo , Akio Ozaki
IPC分类号： C12P7/58 , C12P1/00 , C12Q1/00 , C12N9/24 , C12N1/20 , C02F3/34 , C07K14/00 , C07H21/04
CPC分类号： C12P19/26
摘要： The present invention provides a process for economically producing N-acetylneuraminic acid without using expensive materials such as pyruvic acid and phosphoenolpyruvic acid. The process comprises: allowing (i) a culture of a microorganism having N-acetylneuraminic acid aldolase activity or N-acetylneuraminic acid synthetase activity, or a treated matter of the culture, (ii) a culture of a microorganism capable of producing pyruvic acid or a treated matter of the culture, or a culture of a microorganism capable of producing phosphoenolpyruvic acid or a treated matter of the culture, (iii) N-acetylmannosamine, and (iv) an energy source which is necessary for the formation of pyruvic acid or phosphoenolpyruvic acid to be present in an aqueous medium to form and accumulate N-acetylneuraminic acid in the aqueous medium; and recovering N-acetylneuraminic acid from the aqueous medium.
摘要翻译：本发明提供了经济地生产N-乙酰神经氨酸的方法，而不使用昂贵的材料如丙酮酸和磷酸烯醇丙酮酸。该方法包括：（i）具有N-乙酰神经氨酸醛缩酶活性或N-乙酰神经氨酸合成酶活性的微生物的培养物或培养物的处理物质，（ii）能够产生丙酮酸的微生物培养物或培养物的处理物质，或能够产生磷酸烯醇丙酮酸或培养物处理物质的微生物的培养物，（iii）N-乙酰甘露糖胺，和（iv）形成丙酮酸所必需的能量源或磷酸烯醇式丙酮酸存在于水性介质中以在水性介质中形成并积聚N-乙酰神经氨酸; 并从水性介质中回收N-乙酰神经氨酸。

9. 发明授权

US06964858B2 Processes for producing sugar nucleotides and complex carbohydrates 有权
标题翻译：生产糖核苷酸和复合碳水化合物的方法
公开(公告)号：US06964858B2
公开(公告)日：2005-11-15
申请号：US09907574
申请日：2001-07-19
申请人： Satoshi Koizumi , Katsutoshi Sasaki , Tetsuo Endo , Kazuhiko Tabata , Akio Ozaki
发明人： Satoshi Koizumi , Katsutoshi Sasaki , Tetsuo Endo , Kazuhiko Tabata , Akio Ozaki
IPC分类号： C12P19/30 , C12P1/00 , C07H21/04 , C12N9/00
CPC分类号： C12P19/30
摘要： This invention relates to a process for producing a sugar nucleotide, in which a) a culture broth of a microorganism capable of producing NTP from a nucleotide precursor, or a treated product of the culture broth, and b) a culture broth of a microorganism capable of producing a sugar nucleotide from a sugar and NTP, or a treated product of the culture broth, are used as enzyme sources; a process for producing a complex carbohydrate, in which the above-described a) and b) and c) a culture broth of a microorganism, an animal cell or an insect cell capable of producing a complex carbohydrate from a sugar nucleotide and a complex carbohydrate precursor, or a treated product of the culture broth, are used as enzyme sources; a process for producing a complex carbohydrate, in which a culture broth of a microorganism, an animal cell or an insect cell capable of producing a complex carbohydrate from a sugar nucleotide and a complex carbohydrate precursor, or a treated product of the culture broth, is as an enzyme source; and a process for producing N-acetylglucosamine-1-phosphate, in which a culture broth of a microorganism having strong galactokinase activity, or a treated product of the culture broth, is used as the enzyme source.
摘要翻译：本发明涉及一种制备糖核苷酸的方法，其中a）从核苷酸前体产生NTP的微生物的培养液或培养液的处理产物，和b）能够培养的微生物的培养液从糖和NTP生产糖核苷酸或培养液的处理产物用作酶源; 复合碳水化合物的制造方法，其中上述a）和b）和c）能够从糖核苷酸和复合碳水化合物制备复合碳水化合物的微生物，动物细胞或昆虫细胞的培养液前体或培养液的处理产物用作酶源; 复合碳水化合物的制造方法，其中可以从糖核苷酸和复合碳水化合物前体生产复合碳水化合物的微生物，动物细胞或昆虫细胞的培养液或培养液的处理产物是作为酶源; 使用其中具有强烈的半乳糖激酶活性的微生物的培养液或培养液的处理产物的N-乙酰葡糖胺-1-磷酸的生产方法作为酶源。

10. 发明申请

US20050148048A1 Process for producing dipeptides 审中-公开
标题翻译：二肽生产方法
公开(公告)号：US20050148048A1
公开(公告)日：2005-07-07
申请号：US10995266
申请日：2004-11-24
申请人： Shin-ichi Hashimoto , Kazuhiko Tabata , Ayako Noguchi
发明人： Shin-ichi Hashimoto , Kazuhiko Tabata , Ayako Noguchi
IPC分类号： C12P21/02 , C12P21/06 , C12N9/54
CPC分类号： C12P21/02
摘要： The present invention provides a process for producing a dipeptide which comprises: allowing an enzyme source and one or more kinds of substances selected from the group consisting of amino acid amides, amino acid esters and amino acids to be present in an aqueous medium, said enzyme source being a culture of a microorganism having the ability to produce a dipeptide or a treated matter of the culture, and having been subjected to heat treatment at a temperature of 41 to 65° C. for 30 seconds to one hour so as to produce and accumulate the dipeptide in the aqueous medium; and recovering the dipeptide from the aqueous medium.
摘要翻译：本发明提供了一种制备二肽的方法，其包括：允许酶源和一种或多种选自氨基酸酰胺，氨基酸酯和氨基酸的物质存在于水性介质中，所述酶来源是具有产生培养物的二肽或处理物质的能力的微生物的培养物，并且已经在41至65℃的温度下进行了30秒至1小时的热处理，以便生产和在水性介质中积聚二肽; 并从水性介质中回收二肽。

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