专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明专利

CA1216521A LATEX REAGENT 未知
公开(公告)号：CA1216521A
公开(公告)日：1987-01-13
申请号：CA450972
申请日：1984-03-30
申请人： MITSUBISHI CHEM IND , KAZUE UENO
发明人： UENO KASUE , KOHNO HIDEKI , TSUTSUI SATOSHI
IPC分类号： G01N33/569 , G01N33/543 , A61K39/40
摘要： Latex particles are sensitized with Immunoglobulin G obtained from the antiserum collected from a laboratory animal which has been immunized with a toxin extracted from pathogenic enterobacterium. The IgG-sensitized latex is useful for diagnosis of diseases caused by pathogenic enterobacteria.

2. 发明专利

JPS59183369A Latex reagent 失效
标题翻译： LATEX试剂
公开(公告)号：JPS59183369A
公开(公告)日：1984-10-18
申请号：JP5706083
申请日：1983-04-01
申请人： Mitsubishi Chem Ind Ltd , Kazue Ueno
发明人： UENO KAZUE , JINNO HIDEKI , TSUTSUI TOSHIAKI
IPC分类号： G01N33/569 , G01N33/543
CPC分类号： G01N33/54313
摘要： PURPOSE: To obtain a longterm preservable reagent capable of rapidly detecting the toxin in excrements with high accuracy, by a method wherein an IgG-containing immune globulin fraction is obtained from anti-serum obtained by administering an intestinal bacterial toxin to an animal while latex particles formed by sensitizing said fraction are treated with a protein solution.
CONSTITUTION: For example, in performing the diagnosis of pseudomembranous enteritis caused by toxins D-1, D-2 produced by a intestinal anaerobic bacterium Clostridium difficile, an IgG-containing fraction is obtained from immune serum obtained by administering said toxins and org. high-molecular latex particles such as polystyrene particles are sensitized with said fraction. The sensitized latex is treated with a protein solution such as bovine serum albumin and, after centrifugal separation is performed to remove the supernatant liquid, the obtained particles are suspended in a borate buffer solution to prepare a toxin detecting reagent. This reagent is stable even after a half year and provides stable measuring accuracy. In detecting the toxins, excrements are added to a physiological saline solution and subjected to centrifugal separation to take the supernatant liquid while the reagent is added to a part thereof and the presence or absence of agglutination is measured or the generated turbidity is measured at 940nm by photometry.
COPYRIGHT: (C)1984,JPO&Japio
摘要翻译：目的：为了获得能够高精度快速检测排泄物中的毒素的长期保存试剂，可以通过以下方法得到含有IgG的免疫球蛋白部分的方法：通过向动物施用肠细菌毒素而得到的抗血清，而胶乳粒子通过使所述级分致敏形成的蛋白质溶液进行处理。构成：例如，在由肠型厌氧细菌艰难梭菌产生的毒素D-1，D-2引起的假膜性肠炎的诊断中，通过施用所述毒素和组织获得的免疫血清获得含IgG的级分。高分子胶乳颗粒如聚苯乙烯颗粒用所述部分致敏。将敏化胶乳用牛血清白蛋白等蛋白质溶液处理，离心分离除去上清液后，将得到的粒子悬浮于硼酸盐缓冲溶液中，制备毒素检测试剂。该试剂即使在半年后也是稳定的，提供稳定的测量精度。在检测毒素时，将排泄物加入到生理盐水溶液中并进行离心分离以将试剂添加到其一部分中，并测定凝集物的存在或不存在，或者在940nm处测量产生的浊度测光。

3. 发明专利

JPS58216123A Antiserum 失效
标题翻译：反义词
公开(公告)号：JPS58216123A
公开(公告)日：1983-12-15
申请号：JP9956682
申请日：1982-06-10
申请人： Mitsubishi Chem Ind Ltd , Kazue Ueno
发明人： UENO KAZUE , JINNO HIDEKI , TSUTSUI TOSHIAKI
IPC分类号： A61K39/08 , G01N33/569
摘要： PURPOSE: To prepare a toxin antiserum, by attenuating the toxin produced by Clostridium difficile, and immunizing an animal with the toxin.
CONSTITUTION: A toxin is prepared by the anaerobic cultivation of Clostridium difficile strain which is an anaerobic enteric bacterial strain. After cultivation, the product is centrifuged, and the toxin separated from the supernatant liquid is purified to obtain purified toxins D-1 and D-2. The toxins are attenuated by the treatment with formaldehyde, glutaraldehyde, etc. An animal such as rabbit, guinea pig, goat, etc. is immunized with the toxins to obtain an antiserum having high specificity to D-1 and D-2 toxins. A toxin-detection reagent can be prepared by separating immunoglobulin (IgG) from the antiserum, and sensitizing the IgG to a latex particles such as polystyrene.
COPYRIGHT: (C)1983,JPO&Japio
摘要翻译：目的：制备毒素抗血清，减毒艰难梭菌产生的毒素，并用毒素免疫动物。构成：通过厌氧培养艰难梭菌菌株（厌氧菌肠杆菌菌株）制备毒素。培养后，将产物离心，将从上清液中分离的毒素纯化，得到纯化的毒素D-1和D-2。毒素通过用甲醛，戊二醛等处理而减弱。用毒素免疫兔，豚鼠，山羊等动物，得到对D-1和D-2毒素具有高特异性的抗血清。毒素检测试剂可以通过从抗血清中分离免疫球蛋白（IgG）并使IgG对胶乳颗粒如聚苯乙烯进行敏化来制备。

4. 发明专利

JPS58215558A Latex reagent 失效
标题翻译： LATEX试剂
公开(公告)号：JPS58215558A
公开(公告)日：1983-12-15
申请号：JP9956582
申请日：1982-06-10
申请人： Mitsubishi Chem Ind Ltd , Kazue Ueno
发明人： UENO KAZUE , JINNO HIDEKI , TSUTSUI TOSHIAKI
IPC分类号： A61K39/00 , G01N33/569
CPC分类号： G01N33/56916
摘要： PURPOSE:To obtain a latex reagent for detecting toxins, by fractionating immunoglobulin (IgG) from antiserum against intestinal bacterial toxins, and sensitizing latex particles to IgG. CONSTITUTION:Solid and watery feces from patients of pseudomembranous enteritis are extracted with 0.9% NaCl on solution, and the extract is used as a clindical sample. When IgG the surfaces of latex particles and toxins D-1 and D-2 in the said extract are allowed to be reacted with each other to assay the said toxins, the toxins in patients of enteritis are semiquantitatively detected by observing macroscopically the extent of the aggregation messes of latex on a slide glass by means of a multivalent antibody reaction. Owing to rapidity of the above procedure, judgement of pseudomembranous enteritis caused by Clostridium diflicile is made much more rapidly with higher accuracy in comparison with conventional procedures. When the aggregation reaction of the latex reagent with the toxins in feces from the patients diagnosed with this procedure is compared with the reaction of extract of feces from normal human, the toxins are clearly detected according to the existence of the aggregation masses of the latex reagent, and thus the judgement of pseudomembranous enteritis can be made in the manner clinical examination.
摘要翻译：目的：通过从抗血清中分离免疫球蛋白（IgG）以获得用于检测毒素的胶乳试剂，对肠道细菌毒素进行分级，并将胶乳颗粒对IgG进行敏化。构成：假性膜性肠炎患者的固体和水样粪便用溶液中的0.9％NaCl萃取，提取物用作clindical样品。当IgG使所述提取物中的胶乳颗粒和毒素D-1和D-2的表面彼此反应以测定所述毒素时，通过宏观观察来检测肠炎患者中的毒素，通过多价抗体反应在载玻片上的胶乳聚集混合物。由于上述方法的快速性，与传统方法相比，由梭菌引起的假膜性肠炎的判断更快，准确度更高。当将来自诊断为本方法的患者的乳液试剂与粪便中的毒素的聚集反应与来自正常人的粪便提取物的反应进行比较时，根据胶乳试剂的聚集体的存在可以清楚地检测毒素，因此可以以临床检查的方式进行假膜性肠炎的判断。

5. 发明授权

US4937201A Latex reagent for detection of the toxin of clostridium difficile 失效
标题翻译：用于检测艰难梭菌毒素的乳胶试剂
公开(公告)号：US4937201A
公开(公告)日：1990-06-26
申请号：US495487
申请日：1983-05-17
申请人： Kazue Ueno , Hideki Kohno , Satoshi Tsutsui
发明人： Kazue Ueno , Hideki Kohno , Satoshi Tsutsui
IPC分类号： G01N33/543 , G01N33/569
CPC分类号： G01N33/54393 , G01N33/54313 , G01N33/56916 , Y10S435/842
摘要： Latex particles are sensitized with Immunoglobulin G obtained from the antiserum collected from a laboratory animal which has been immunized with a toxin extracted from pathogenic enterobacterium. The IgG-sensitized latex is useful for diagnosis of diseases caused by pathogenic enterobacteria.
摘要翻译：用从用从病原性肠杆菌提取的毒素免疫的实验室动物中收集的抗血清获得的免疫球蛋白G使乳胶颗粒致敏。 IgG致敏乳胶可用于诊断由致病肠杆菌引起的疾病。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式