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1. 发明公开

EP1608977A1 NANOPARTICLE FOR BIOAFFINITY ASSAYS 有权
标题翻译：纳米粒子BIOAFFINITÄTSTESTS
公开(公告)号：EP1608977A1
公开(公告)日：2005-12-28
申请号：EP04725404.0
申请日：2004-04-02
申请人： SOUKKA, Tero , Korpimäki, Teemu , Lamminmäki, Urpo , Virta, Marko
发明人： SOUKKA, Tero , Korpimäki, Teemu , Lamminmäki, Urpo , Virta, Marko
IPC分类号： G01N33/543 , G01N33/58
CPC分类号： G01N33/6845 , C07K14/47 , G01N33/543 , G01N33/54346 , G01N33/587 , G01N33/68
摘要： Thus this invention relates to a nanoparticle, useful for bioaffinity assays. The nanoparticle has a self-assembling shell built up of several protein and/or peptide subunits, which protein and/or peptide subunits can be of one or several different types, assembled in an organized manner to form the shell having an inner surface facing the inside and an outer surface facing the outside of said particle. One or several of the types of subunits have one or several first binding moieties per type of subunit with the binding moiety facing the outside of the particle for binding of any specific ligand binding protein; and the particle contains within its shell a marker and/or one or several of the types of subunits have one or several second binding moieties per type of subunit with the binding moiety facing the inside and/or the outside of the particle for binding a marker; and the marker or markers enables detection of the particle. Characteristic for the nanoparticle is that the shell of the nanoparticle is a recombinant apoferritin or an apoferritin-like particle. This invention also relates to a bioaffinity assays using the nanoparticle. This invention further relates to a kit for bioaffinity assays comprising the nanoparticle.

2. 发明申请

WO2005049206A1 NUCLEIC ACID AMPLIFICATION ASSAY AND ARRANGEMENT THEREFOR 审中-公开
标题翻译：核酸放大测定及其安排
公开(公告)号：WO2005049206A1
公开(公告)日：2005-06-02
申请号：PCT/FI2004/000678
申请日：2004-11-15
申请人： NURMI, Jussi , KORPIMÄKI, Teemu
发明人： NURMI, Jussi , KORPIMÄKI, Teemu
IPC分类号： B01L3/00
CPC分类号： B01L3/502 , B01L2200/0647 , B01L2300/0681 , B01L2400/0622 , B01L2400/0644 , G01N1/40 , G01N1/4077
摘要： The present invention relates to a nucleic acid amplification assay for quantitative and/or qualitative analysis of the presence of a specific analyte or specific analytes in a biological sample, which analytes, if present, are contained in particles (4) of said sample (2), in which assay the sample is forced in a first direction through a filter (6) that retains said particles (4). Characteristic for the method is that the particles (4) retained in the filter (6) are flushed, by a flow (8), in a second opposite direction through the filter (6) out of the filter (6) and the flow (8) containing said particles (4) flushed out is analysed for the analyte or analytes. The invention further relates to an arrangement (12) for preparing the sample (2) for analysis according to the assay of the invention and to a kit of parts for analysing the analyte or analytes, which kit comprises the arrangement (12).
摘要翻译：本发明涉及用于定量和/或定性分析生物样品中特定分析物或特异性分析物的存在的核酸扩增测定法，该分析物（如果存在）包含在所述样品（2）的颗粒（4）中），在该测定中，样品通过保持所述颗粒（4）的过滤器（6）沿第一方向被迫。该方法的特征在于，保留在过滤器（6）中的颗粒（4）通过流动（8）在过滤器（6）和过滤器（6）之间的过滤器（6）的第二相反方向上被冲洗 8）含有所述粉碎的颗粒（4）被分析分析物或分析物。本发明还涉及一种用于制备用于根据本发明的测定分析的样品（2）和用于分析分析物或分析物的试剂盒的装置（12），该试剂盒包括该装置（12）。

3. 发明申请

WO2004088313A1 NANOPARTICLE FOR BIOAFFINITY ASSAYS 审中-公开
标题翻译：用于生物测定的纳米颗粒
公开(公告)号：WO2004088313A1
公开(公告)日：2004-10-14
申请号：PCT/FI2004/000204
申请日：2004-04-02
申请人： SOUKKA, Tero , KORPIMÄKI, Teemu , LAMMINMÄKI, Urpo , VIRTA, Marko
发明人： SOUKKA, Tero , KORPIMÄKI, Teemu , LAMMINMÄKI, Urpo , VIRTA, Marko
IPC分类号： G01N33/543
CPC分类号： G01N33/6845 , C07K14/47 , G01N33/543 , G01N33/54346 , G01N33/587 , G01N33/68
摘要： Thus this invention relates to a nanoparticle, useful for bioaffinity assays. The nanoparticle has a self-assembling shell built up of several protein and/or peptide subunits, which protein and/or peptide subunits can be of one or several different types, assembled in an organized manner to form the shell having an inner surface facing the inside and an outer surface facing the outside of said particle. One or several of the types of subunits have one or several first binding moieties per type of subunit with the binding moiety facing the outside of the particle for binding of any specific ligand binding protein; and the particle contains within its shell a marker and/or one or several of the types of subunits have one or several second binding moieties per type of subunit with the binding moiety facing the inside and/or the outside of the particle for binding a marker; and the marker or markers enables detection of the particle. Characteristic for the nanoparticle is that the shell of the nanoparticle is a recombinant apoferritin or an apoferritin-like particle. This invention also relates to a bioaffinity assays using the nanoparticle. This invention further relates to a kit for bioaffinity assays comprising the nanoparticle.
摘要翻译：因此，本发明涉及可用于生物亲和测定的纳米颗粒。纳米颗粒具有由几个蛋白质和/或肽亚基构成的自组装壳，所述蛋白质和/或肽亚基可以是一种或多种不同类型，以有组织的方式组装形成具有面向内部和面向所述颗粒外部的外表面。亚单位中的一种或几种具有每种亚基具有一个或几个第一结合部分，其结合部分面向颗粒外部，用于结合任何特异性配体结合蛋白; 并且所述颗粒在其壳内包含标记物和/或所述亚单位的一种或几种，每种亚单位具有一个或多个第二结合部分，所述结合部分面向颗粒的内部和/或外部，用于结合标记 ; 标记或标记使得能够检测到颗粒。纳米颗粒的特征是纳米颗粒的壳是重组脱铁铁蛋白或脱铁铁蛋白样颗粒。本发明还涉及使用纳米颗粒的生物亲和力测定。本发明还涉及包含纳米颗粒的生物亲和力测定试剂盒。

4. 发明申请

WO2007063174A1 ENRICHMENT UNIT FOR BIOLOGICAL COMPONENTS AND AN ENRICHMENT METHOD 审中-公开
标题翻译：生物组分生长单元和增产方法
公开(公告)号：WO2007063174A1
公开(公告)日：2007-06-07
申请号：PCT/FI2006/000403
申请日：2006-12-04
申请人： OY BIO-NOBILE OY , RAISIO DIAGNOSTICS OY , KORPELA, Matti , MIETTINEN, Tytti , TENHUNEN, Tuija , KORPIMÄKI, Teemu , MATTSSON, Pekka , TUOMOLA, Mika
发明人： KORPELA, Matti , MIETTINEN, Tytti , TENHUNEN, Tuija , KORPIMÄKI, Teemu , MATTSSON, Pekka , TUOMOLA, Mika
IPC分类号： B03C1/28 , C12M1/00 , C12M1/26 , C12M1/42 , C12Q1/24 , C12Q1/68 , G01N1/02 , G01N1/40 , G01N33/543 , G01N35/00
CPC分类号： G01N33/54326 , B03C1/01 , B03C1/286 , C12M47/02 , C12M47/04 , G01N1/40 , G01N35/0098
摘要： A biological component enrichment unit for the isolation, purification and/or determination of a biological component using particles (3) and/or another solid phase, which enrichment unit comprises at least one sample container (1 , 15, 26, 32, 39) for the realisation of the biological sample method. The enrichment unit comprises a lead-through structure (5), such as a bushing (21 , 27) made up of one or several parts or a cover having one or several openings (25, 36). The lead-through structure and one or several sample containers can be brought into connection with one another so that, when the lead-through structure is placed on top of the sample container, a functional unit is formed for the realisation of the biological method, such as for the binding of the component, the growing, isolation, purification, enrichment of the bacteria or cell or for another similar method.
摘要翻译：一种用于使用颗粒（3）和/或另一种固相分离，纯化和/或确定生物组分的生物组分富集单元，所述富集单元包括至少一个样品容器（1,15,26,32,39）用于实现生物样本法。浓缩单元包括一个引导结构（5），例如由一个或多个部分构成的衬套（21,27）或具有一个或多个开口（25,36）的盖子。引线结构和一个或多个样品容器可以彼此连接，使得当引线结构放置在样品容器的顶部时，形成用于实现生物学方法的功能单元，例如组分的结合，细菌或细胞的生长，分离，纯化，富集或另外类似的方法。

5. 发明申请

WO2010059952A1 METHOD FOR DETERMINING THE RISK OF PREECLAMPSIA USING PIGF-2 AND PIGF-3 MARKERS 审中-公开
标题翻译：使用PIGF-2和PIGF-3标记物确定前列腺炎风险的方法
公开(公告)号：WO2010059952A1
公开(公告)日：2010-05-27
申请号：PCT/US2009/065355
申请日：2009-11-20
申请人： PERKINELMER HEALTH SCIENCES, INC. , NTD LABORATORIES, INC. , WALLAC OY , AHOLA, Tarja , FRANG, Heini , KORPIMÄKI, Teemu , HURSKAINEN, Pertti , BOBROW, Mark N. , CARMICHAEL, Jonathan B.
发明人： AHOLA, Tarja , FRANG, Heini , KORPIMÄKI, Teemu , HURSKAINEN, Pertti , BOBROW, Mark N. , CARMICHAEL, Jonathan B.
IPC分类号： G01N33/68
CPC分类号： G01N33/689 , G01N2800/368 , G06F19/28
摘要： The present invention relates to a method for determining the risk of a pregnant woman developing pre-eclampsia. The method comprises i) determining the level of one or more biochemical markers in a sample obtained from a pregnant woman, and ii) comparing the level of the at least one biochemical marker in the sample with the level of the same biochemical marker in a control sample. A difference in the level of the biochemical marker in the sample relative to the control sample is indicative of an increased risk of developing pre-eclampsia. The isoform biochemical markers are preferably PlGF-2 and PlGF-3. The present invention relates also to a method for determining whether a pregnant woman has pre-eclampsia and as well as a kit for assessing the risk or presence of pre-eclampsia. In addition, the invention relates also to a computer program used in these determinations.
摘要翻译：本发明涉及一种确定孕妇发生先兆子痫风险的方法。该方法包括：i）确定从孕妇获得的样品中的一种或多种生物化学标志物的水平，和ii）将样品中的至少一种生化标志物的水平与对照中相同生物化学标记物的水平进行比较样品。相对于对照样品，样品中生化标志物水平的差异表明发生先兆子痫的风险增加。同种型生化标志物优选为PlGF-2和PlGF-3。本发明还涉及一种用于确定孕妇是否具有先兆子痫的方法，以及用于评估先兆子痫的风险或存在的试剂盒。此外，本发明还涉及在这些确定中使用的计算机程序。

6. 发明申请

WO2005118849A1 METHOD FOR STABILIZING ASSAY REAGENTS, REAGENT CONTAINER WITH STABILIZED ASSAY REAGENTS AND USE THEREOF 审中-公开
标题翻译：用于稳定测试试剂的方法，具有稳定的测定试剂的试剂容器及其用途
公开(公告)号：WO2005118849A1
公开(公告)日：2005-12-15
申请号：PCT/FI2005/050194
申请日：2005-06-03
申请人： ABACUS DIAGNOSTICA OY , KORPIMÄKI, Teemu , LÖVGREN, Timo , NURMI, Jussi
发明人： KORPIMÄKI, Teemu , LÖVGREN, Timo , NURMI, Jussi
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6848 , B01L3/50851 , B01L2200/16 , B01L2300/0829 , B01L2300/16 , C12Q1/686 , C12Q2547/101
摘要： This invention relates to a reagent container for detection and/or quantitation of at least one biological or chemical analyte from a sample, said reagent container comprising an inner surface enclosing a volume, wherein volume analytical reactions of at least one analysis for detection and/or quantitation of at least one analyte take place, and at least two reagents of an analysis of an analyte have been dried onto said inner surface and at least a first said reagent has been dried onto a first area of the inner surface distinctly separate, i.e. without any overlap, from a second area of the inner surface onto which at least a second said reagent has been dried. Characteristic for the reagent container is that the first reagent and the second reagent form a pair wherein the first reagent is an enzyme and the second reagent is a substrate of said enzyme, and said pair consists of a nucleic acid polymerase and substrate thereof. This invention also relates to a method for stabilising onto an inner surface of a reagent container dried assay reagents for the detection and/or quantitation of a biological or chemical analyte from a sample, said method comprising the steps of dispensing at least two reagents, a first reagent and a second reagent, needed in the detection of the analyte onto the inner surface of the reagent container; and removing excess water from the reagents, wherein first reagent is dispensed onto a first area of the inner surface distinctly separate, i.e. without any overlap, from a second area of the inner surface onto which the second reagent is dispensed. Characteristic for the method is that the first reagent and the second reagent form a pair wherein the first reagent is an enzyme and the second reagent is a substrate of said enzyme, and said pair consist of a nucleic acid polymerase and substrate thereof. This invention further relates to the use of the reagent container to perform an assay selected from the group consisting of a polymerase chain reaction (PCR) assay, an assay that utilizes a reverse transcriptase, a reverse transriptase polymerase chain reaction, an immuno-PCR assay, a nucleic acid sequence based assay (NASBA), a proximity ligation assay, a ligase chain reaction (LCR) assay, a rolling circle amplification (RCA) assay, and a strand displacement amplification (SDA) assay.
摘要翻译：本发明涉及一种用于检测和/或定量来自样品的至少一种生物或化学分析物的试剂容器，所述试剂容器包括封闭体积的内表面，其中至少一个用于检测的分析物的体积分析反应和/或对至少一种分析物的定量进行，并且分析物的分析的至少两种试剂已经干燥到所述内表面上，并且至少第一种所述试剂已经被干燥到内表面的第一区域上，分离开，即没有从内表面的至少第二所述试剂已被干燥到的第二区域的任何重叠。试剂容器的特征在于第一试剂和第二试剂形成一对，其中第一试剂是酶，第二试剂是所述酶的底物，并且所述对由核酸聚合酶及其底物组成。本发明还涉及一种用于稳定试剂容器内表面的方法，用于从样品中检测和/或定量生物或化学分析物，所述方法包括以下步骤：分配至少两种试剂，第一试剂和第二试剂，用于将分析物检测到试剂容器的内表面上; 以及从所述试剂中除去多余的水，其中第一试剂被分配到所述内表面的第一区域上，所述内表面的第一区域与所述第二试剂被分配到的内表面的第二区域分开，即没有任何重叠。该方法的特征在于，第一试剂和第二试剂形成一对，其中第一试剂是酶，第二试剂是所述酶的底物，并且所述对由核酸聚合酶及其底物组成。本发明还涉及试剂容器用于进行选自聚合酶链反应（PCR）测定，利用逆转录酶的测定，反向转录酶聚合酶链反应，免疫-PCR测定，基于核酸序列的测定（NASBA），接近连接测定，连接酶链反应（LCR）测定，滚环扩增（RCA）测定和链置换扩增（SDA）测定。

7. 发明申请

WO2005118144A1 TEMPERATURE CONTROL OF REACTION VESSEL, SYSTEM WITH REACTION VESSEL, SOFTWARE PRODUCT FOR SYSTEM AND USE OF SYSTEM 审中-公开
标题翻译：反应容器的温度控制，反应容器系统，系统软件产品和系统的使用
公开(公告)号：WO2005118144A1
公开(公告)日：2005-12-15
申请号：PCT/FI2005/050196
申请日：2005-06-03
申请人： ABACUS DIAGNOSTICA OY , NURMI, Jussi , KORPIMÄKI, Teemu , LÖVGREN, Timo , KOJOLA, Hannu , IITIÄ, Antti
发明人： NURMI, Jussi , KORPIMÄKI, Teemu , LÖVGREN, Timo , KOJOLA, Hannu , IITIÄ, Antti
IPC分类号： B01L7/00
CPC分类号： G05D23/192 , B01L3/5027 , B01L7/5255 , B01L2200/142 , B01L2200/147 , B01L2300/0816 , B01L2300/0887 , B01L2300/1805
摘要： The invention relates to a method for rapid thermal control of a reaction volume (4) of a reaction vessel (2) for a change of temperature of the reaction volume (4) from a preceding temperature to a target temperature. Characteristic for the method is that the change of temperature is carried out by first bringing at least the second wall (8) of a second material with a high thermal conductivity of the reaction vessel (2) in direct contact with a first thermal block (14-19), at a temperature higher than the target temperature if the target temperature is higher than the preceding temperature, or correspondingly at a temperature lower than the target temperature if the target temperature is lower than the preceding temperature, for a time needed to bring the temperature of the reaction volume (4) to or close to the target temperature; and then bringing at least the second wall (8) in direct contact with a second thermal block (14-19) at the target temperature to bring the reaction volume (4) to the target temperature and/or keep the reaction volume (4) at the target temperature for the time intended. The first thermal block (14-19) is at a constant predetermined first temperature and the second thermal block (14-19) is at a constant predetermined second temperature. The invention also relates to a system (20) for detecting and/or quantitating a biological and/or chemical analyte or analytes in a sample supposedly containing said analyte or analytes for carrying out the method. The invention further relates to a software product for the system (20) and uses of the system (20).
摘要翻译：本发明涉及一种快速热控制反应容器（2）的反应体积（4）的方法，用于将反应体积（4）的温度从先前温度改变到目标温度。该方法的特征在于，通过首先使具有与第一热块（14）直接接触的反应容器（2）的高热导率的第二材料的至少第二壁（8） -19），如果目标温度高于先前温度，或者如果目标温度低于先前温度，则相应地在比目标温度低的温度下，在高于目标温度的温度下，持续所需的时间反应体积（4）的温度达到或接近目标温度; 然后使至少第二壁（8）与目标温度下的第二热块（14-19）直接接触以使反应体积（4）达到目标温度和/或保持反应体积（4）在目标温度下预期的时间。第一热块（14-19）处于恒定的预定第一温度，第二热块（14-19）处于恒定的预定第二温度。本发明还涉及一种用于检测和/或定量假定含有所述分析物或样品的样品中的生物和/或化学分析物或分析物用于实施该方法的系统（20）。本发明还涉及用于系统（20）的软件产品和系统（20）的使用。

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