专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明专利

DE10112608A1 New non-naturally occurring variant of glutaryl amidase comprising histidine or glutamate in its substrate binding pocket, binds cephalosporin C (CPC) as substrate and catalyzes conversion of CPC to 7-aminocephalosporanic acid 未知
公开(公告)号：DE10112608A1
公开(公告)日：2002-09-19
申请号：DE10112608
申请日：2001-03-14
申请人： KOLLER KLAUS-PETER
发明人： KOLLER KLAUS-PETER , LANGE GUDRUN , SAUBER KLAUS , FRITZ-WOLF KARIN , KABSCH WOLFGANG
IPC分类号： C07D501/28 , C12N9/80 , C12N15/55 , C12P35/00 , C12N9/14 , C07D519/06 , C12P17/18 , C12P35/06
摘要： Non-naturally occurring variant of a glutaryl amidase (GA) (I) which binds cephalosporin C (CPC) as a substrate and catalyzes conversion of the CPC to 7-aminocephalosporanic acid (7-ACA), where the variant comprises a histidine or glutamate in the substrate binding pocket that binds the alpha -amino adipyl moiety of the CPC, is new. A non-naturally occurring variant of a glutaryl amidase (GA) (I) which binds cephalosporin C (CPC) as a substrate and catalyzes conversion of the CPC to 7-aminocephalosporanic acid (7-ACA), where the variant comprises a histidine or glutamate in the substrate binding pocket that binds the alpha -amino adipyl moiety of the CPC. (I) comprises a combination of mutations chosen from PheB177His, LeuB24Arg, ArgB57His; PheB177His, LeuB24Arg, ArgB57His, GlnB50Val; PheB177His, LeuB24Arg, ArgB57His, GlnB50Ala; PheB177His, LeuB24Arg, ArgB57His, GlnB50Val, ValB70His; PheB177His, LeuB24Arg, ArgB57His, GlnB50Ala, ValB70His; PheB177His, LeuB24Arg, ArgB57His, GlnB50Val, ValB70His, TyrB153Leu; PheB177His, LeuB24Arg, ArgB57His, GlnB50Ala, ValB70His, TyrB153Leu; PheB177His, ThrB176Asp; PheB177His, ThrB176Asp, TyrA150Ala; PheB177His, ThrB176Asp, LeuB24Arg, ArgB57His; PheB177His, ThrB176Asp, LeuB24Arg, ArgB57His, TyrA150Ala; PheB177His, ThrB176Asp, GlnB50Arg, ArgB57His; PheB177His, ThrB176Asp, GlnB50Arg, ArgB57His, TyrA150Ala; TyrA150Glu; TyrA150Glu, PheB177His; TyrA150Glu, LeuB24Arg, ArgB57His; TyrA150Glu, LeuB24Arg, ArgB57His, PheB177His; TyrA150Glu, GlnB50Arg, ArgB57His; TyrA150Glu, GlnB50Arg, ArgB57His, PheB177His; PheB177His, ThrB176Asp, TyrA150Ala, TyrB33Ser; PheB177His, ThrB176Asp, LeuB24Arg, ArgB57His, TyrA150Ala, TyrB33Ser; PheB177His, ThrB176Asp, GlnB50Arg, ArgB57His, TyrA150Ala, TyrB33Ser; TyrA150Glu, PheB177His, TyrB33Ser; TyrA150Glu, LeuB24Arg, ArgB57His, TyrB33Ser; PheB177His, LeuB24Arg, ArgB57His, TyrB33Ser; and PheB177His, LeuB24Arg, ArgB57His, TyrB33Ser, ValB70His. Independent claims are also included for: (1) a polynucleotide (II) encoding (I); (2) a nucleic acid vector (III) comprising (II); (3) a host cell (IV) comprising (II) operably linked to regulatory control sequence which direct expression of the variant GA in the host cell; (4) production of (I); (5) production of 7-aminocephalosporanic acid; and (6) modifying (M1) a GA enzyme to enhance the CPC acylase activity of the enzyme, which involves by introducing a non-naturally occurring histidine or glutamate amino acid residue in the substrate binding pocket of the enzyme which histidine or glutamate binds the alpha -amino adipyl moiety of a CPC substrate molecule.

2. 发明专利

SK279686B6 PROCESS FOR HYDROLYSIS OF AMINO-ACID CHAIN OF PREPROINSULINS 未知
公开(公告)号：SK279686B6
公开(公告)日：1999-02-11
申请号：SK271191
申请日：1991-09-03
申请人： DORSCHUG MICHAEL , KOLLER KLAUS-PETER , MARQUARDT RUDIGER , MEIWES JOHANNES
发明人： DORSCHUG MICHAEL , KOLLER KLAUS-PETER , MARQUARDT RUDIGER , MEIWES JOHANNES
IPC分类号： C12N9/52 , C07K14/62 , C12N9/48 , C12N15/09 , C12P21/02 , C12P21/06
摘要： A process for the specific hydrolysis of the amino-acid chain of preproinsulins to give the corresponding intermediates with the aid of clostripain is described, and these intermediates can be converted where appropriate with carboxypeptidase B into the corresponding insulins.

3. 发明专利

DE10148723A1 New non-naturally occurring variant of glutaryl amidase comprising histidine or glutamate in its substrate binding pocket, binds cephalosporin C (CPC) as substrate and catalyzes conversion of CPC to 7-aminocephalosporanic acid 未知
公开(公告)号：DE10148723A1
公开(公告)日：2003-04-10
申请号：DE10148723
申请日：2001-10-02
申请人： KOLLER KLAUS-PETER
发明人： KOLLER KLAUS-PETER , LANGE GUDRUN , SAUBER KLAUS , FRITZ-WOLF KARIN
IPC分类号： C07D501/02 , C12N9/80 , C12N15/55 , C12P35/00 , C12N9/86
摘要： Non-naturally occurring variant of a glutaryl amidase (GA) (I) which binds cephalosporin C (CPC) as a substrate and catalyzes conversion of the CPC to 7-aminocephalosporanic acid (7-ACA), where the variant comprises a histidine or glutamate in the substrate binding pocket that binds the alpha -amino adipyl moiety of the CPC, is new. A non-naturally occurring variant of a glutaryl amidase (GA) (I) which binds cephalosporin C (CPC) as a substrate and catalyzes conversion of the CPC to 7-aminocephalosporanic acid (7-ACA), where the variant comprises a histidine or glutamate in the substrate binding pocket that binds the alpha -amino adipyl moiety of the CPC. (I) comprises a combination of mutations chosen from PheB177His, LeuB24Arg, ArgB57His; PheB177His, LeuB24Arg, ArgB57His, GlnB50Val; PheB177His, LeuB24Arg, ArgB57His, GlnB50Ala; PheB177His, LeuB24Arg, ArgB57His, GlnB50Val, ValB70His; PheB177His, LeuB24Arg, ArgB57His, GlnB50Ala, ValB70His; PheB177His, LeuB24Arg, ArgB57His, GlnB50Val, ValB70His, TyrB153Leu; PheB177His, LeuB24Arg, ArgB57His, GlnB50Ala, ValB70His, TyrB153Leu; PheB177His, ThrB176Asp; PheB177His, ThrB176Asp, TyrA150Ala; PheB177His, ThrB176Asp, LeuB24Arg, ArgB57His; PheB177His, ThrB176Asp, LeuB24Arg, ArgB57His, TyrA150Ala; PheB177His, ThrB176Asp, GlnB50Arg, ArgB57His; PheB177His, ThrB176Asp, GlnB50Arg, ArgB57His, TyrA150Ala; TyrA150Glu; TyrA150Glu, PheB177His; TyrA150Glu, LeuB24Arg, ArgB57His; TyrA150Glu, LeuB24Arg, ArgB57His, PheB177His; TyrA150Glu, GlnB50Arg, ArgB57His; TyrA150Glu, GlnB50Arg, ArgB57His, PheB177His; PheB177His, ThrB176Asp, TyrA150Ala, TyrB33Ser; PheB177His, ThrB176Asp, LeuB24Arg, ArgB57His, TyrA150Ala, TyrB33Ser; PheB177His, ThrB176Asp, GlnB50Arg, ArgB57His, TyrA150Ala, TyrB33Ser; TyrA150Glu, PheB177His, TyrB33Ser; TyrA150Glu, LeuB24Arg, ArgB57His, TyrB33Ser; PheB177His, LeuB24Arg, ArgB57His, TyrB33Ser; and PheB177His, LeuB24Arg, ArgB57His, TyrB33Ser, ValB70His. Independent claims are also included for: (1) a polynucleotide (II) encoding (I); (2) a nucleic acid vector (III) comprising (II); (3) a host cell (IV) comprising (II) operably linked to regulatory control sequence which direct expression of the variant GA in the host cell; (4) production of (I); (5) production of 7-aminocephalosporanic acid; and (6) modifying (M1) a GA enzyme to enhance the CPC acylase activity of the enzyme, which involves by introducing a non-naturally occurring histidine or glutamate amino acid residue in the substrate binding pocket of the enzyme which histidine or glutamate binds the alpha -amino adipyl moiety of a CPC substrate molecule.

4. 发明专利

AU2002256860A1 Variant glutaryl amidase (cephalosporin acylase) and uses thereof 未知
公开(公告)号：AU2002256860A1
公开(公告)日：2002-09-24
申请号：AU2002256860
申请日：2002-03-12
申请人： MAX PLANCK GESELLSCHAFT , SAUBER KLAUS , LANGE GUDRUN , KOLLER KLAUS PETER
发明人： SAUBER KLAUS , KOLLER KLAUS-PETER , LANGE GUDRUN , FRITZ-WOLF KARIN , KABSCH WOLFGANG
IPC分类号： C12N9/80 , C12P35/00

5. 发明专利

DE8700835U1 未知
公开(公告)号：DE8700835U1
公开(公告)日：1987-03-12
申请号：DE8700835
申请日：1987-01-19
申请人： KOLLER, KLAUS-PETER, DR., 6232 BAD SODEN, DE
IPC分类号： B62H3/12 , E04H6/42

6. 发明专利

DE10153389A1 New non-naturally occurring variant of glutaryl amidase comprising histidine or glutamate in its substrate binding pocket, binds cephalosporin C (CPC) as substrate and catalyzes conversion of CPC to 7-aminocephalosporanic acid 未知
公开(公告)号：DE10153389A1
公开(公告)日：2003-05-15
申请号：DE10153389
申请日：2001-10-31
申请人： KOLLER KLAUS-PETER
发明人： KOLLER KLAUS-PETER , LANGE GUDRUN , SAUBER KLAUS , FRITZ-WOLF KARIN , KABSCH WOLFGANG
IPC分类号： C12N9/80 , C12N15/55 , C12P35/00
摘要： Non-naturally occurring variant of a glutaryl amidase (GA) (I) which binds cephalosporin C (CPC) as a substrate and catalyzes conversion of the CPC to 7-aminocephalosporanic acid (7-ACA), where the variant comprises a histidine or glutamate in the substrate binding pocket that binds the alpha -amino adipyl moiety of the CPC, is new. A non-naturally occurring variant of a glutaryl amidase (GA) (I) which binds cephalosporin C (CPC) as a substrate and catalyzes conversion of the CPC to 7-aminocephalosporanic acid (7-ACA), where the variant comprises a histidine or glutamate in the substrate binding pocket that binds the alpha -amino adipyl moiety of the CPC. (I) comprises a combination of mutations chosen from PheB177His, LeuB24Arg, ArgB57His; PheB177His, LeuB24Arg, ArgB57His, GlnB50Val; PheB177His, LeuB24Arg, ArgB57His, GlnB50Ala; PheB177His, LeuB24Arg, ArgB57His, GlnB50Val, ValB70His; PheB177His, LeuB24Arg, ArgB57His, GlnB50Ala, ValB70His; PheB177His, LeuB24Arg, ArgB57His, GlnB50Val, ValB70His, TyrB153Leu; PheB177His, LeuB24Arg, ArgB57His, GlnB50Ala, ValB70His, TyrB153Leu; PheB177His, ThrB176Asp; PheB177His, ThrB176Asp, TyrA150Ala; PheB177His, ThrB176Asp, LeuB24Arg, ArgB57His; PheB177His, ThrB176Asp, LeuB24Arg, ArgB57His, TyrA150Ala; PheB177His, ThrB176Asp, GlnB50Arg, ArgB57His; PheB177His, ThrB176Asp, GlnB50Arg, ArgB57His, TyrA150Ala; TyrA150Glu; TyrA150Glu, PheB177His; TyrA150Glu, LeuB24Arg, ArgB57His; TyrA150Glu, LeuB24Arg, ArgB57His, PheB177His; TyrA150Glu, GlnB50Arg, ArgB57His; TyrA150Glu, GlnB50Arg, ArgB57His, PheB177His; PheB177His, ThrB176Asp, TyrA150Ala, TyrB33Ser; PheB177His, ThrB176Asp, LeuB24Arg, ArgB57His, TyrA150Ala, TyrB33Ser; PheB177His, ThrB176Asp, GlnB50Arg, ArgB57His, TyrA150Ala, TyrB33Ser; TyrA150Glu, PheB177His, TyrB33Ser; TyrA150Glu, LeuB24Arg, ArgB57His, TyrB33Ser; PheB177His, LeuB24Arg, ArgB57His, TyrB33Ser; and PheB177His, LeuB24Arg, ArgB57His, TyrB33Ser, ValB70His. Independent claims are also included for: (1) a polynucleotide (II) encoding (I); (2) a nucleic acid vector (III) comprising (II); (3) a host cell (IV) comprising (II) operably linked to regulatory control sequence which direct expression of the variant GA in the host cell; (4) production of (I); (5) production of 7-aminocephalosporanic acid; and (6) modifying (M1) a GA enzyme to enhance the CPC acylase activity of the enzyme, which involves by introducing a non-naturally occurring histidine or glutamate amino acid residue in the substrate binding pocket of the enzyme which histidine or glutamate binds the alpha -amino adipyl moiety of a CPC substrate molecule.

7. 发明申请

WO2002072806A2 MUTANT GLUTARYL AMIDASE AND USES THEREOF 审中-公开
标题翻译： MUTANT GLUTARYL AMIDASE及其用途
公开(公告)号：WO2002072806A2
公开(公告)日：2002-09-19
申请号：PCT/IB2002/002119
申请日：2002-03-12
申请人： MAX-PLANCK-GESELLSCHAFT ZUR FÖRDERUNG DER WISSCNSCHAFTEN E.V. , KOLLER, Klaus-Peter , LANGE, Gudrun , SAUBER, Klaus , FRITZ-WOLF, Karin , KABSCH, Wolfgang
发明人： KOLLER, Klaus-Peter , LANGE, Gudrun , SAUBER, Klaus , FRITZ-WOLF, Karin , KABSCH, Wolfgang
IPC分类号： C12N9/00
CPC分类号： C12P35/00 , C12N9/80
摘要： Variants of glutaryl amidase are described and a process for the preparation of 7-aminocephalosporanic acid using the same.
摘要翻译：描述戊二酰胺酶的变体和使用其制备7-氨基头孢烷酸的方法。

8. 发明申请

WO02072806A3 VARIANT GLUTARYL AMIDASE CEPHALOSPORIN ACYLASE AND USES THEREOF 审中-公开
标题翻译：变种葡聚糖氨基磷酸酯酶及其用途
公开(公告)号：WO02072806A3
公开(公告)日：2003-05-30
申请号：PCT/IB0202119
申请日：2002-03-12
申请人： MAX PLANCK GESELLSCHAFT , KOLLER KLAUS-PETER , LANGE GUDRUN , SAUBER KLAUS , FRITZ-WOLF KARIN , KABSCH WOLFGANG
发明人： KOLLER KLAUS-PETER , LANGE GUDRUN , SAUBER KLAUS , FRITZ-WOLF KARIN , KABSCH WOLFGANG
IPC分类号： C12N9/80 , C12P35/00
CPC分类号： C12P35/00 , C12N9/80
摘要： Variants of glutaryl amidase are described and a process for the preparation of 7-aminocephalosporanic acid using the same.
摘要翻译：描述戊二酰胺酶的变体和使用其制备7-氨基头孢烷酸的方法。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式