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    • 2. 发明申请
    • AN alpha-GALACTOSIDASE ENZYME
    • 阿尔法糖皮质激素酶
    • WO1994023022A1
    • 1994-10-13
    • PCT/DK1994000138
    • 1994-03-30
    • NOVO NORDISK A/SKNAP, Inge, HelmerHJORT, Carsten, M.HALKIER, TorbenKOFOD, Lene, Venke
    • NOVO NORDISK A/S
    • C12N09/40
    • C12N9/2465
    • A DNA construct comprising a DNA sequence encoding a polypeptide having alpha -galactosidase activity, wherein the DNA sequence a) encodes a polypeptide comprising the amino acid sequence shown in the appended SEQ ID No. 3, or b) is an analogue of the DNA sequence of a), which i) hybridizes with the DNA sequence shown in the appended SEQ ID No. 1 or 2 or an oligonucleotide probe prepared on the basis of said DNA sequence or on the basis of the amino acid sequence shown in SEQ ID No. 3 under the conditions defined herein; ii) encodes a polypeptide reactive with an antibody reacting with at least one epitope of a polypeptide comprising the amino acid sequence shown in the appended SEQ ID No. 3, and/or; iii) encodes a polypeptide being at least 50 % identical with the polypeptide having the amino acid sequence shown in the appended SEQ ID No. 3, as well as an alpha -galactosidase enzyme encoded by the DNA construct.
    • 包含编码具有α-半乳糖苷酶活性的多肽的DNA序列的DNA构建体,其中所述DNA序列a)编码包含所附SEQ ID No.3所示氨基酸序列的多肽,或b)是所述DNA序列的类似物 的a),其i)与所附的SEQ ID No.1或2所示的DNA序列或基于所述DNA序列制备的寡核苷酸探针杂交,或者基于SEQ ID NO:1所示的氨基酸序列 3在本文定义的条件下; ii)编码与与包含所附SEQ ID No.3所示的氨基酸序列的多肽的至少一个表位反应的抗体反应的多肽,和/或; iii)编码与所附的SEQ ID No.3所示的氨基酸序列的多肽具有至少50%同一性的多肽,以及由DNA构建体编码的α-半乳糖苷酶。