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1. 发明申请

WO1994008242A1 PROCESS FOR ELIMINATING LABILE GLYCOHAEMOGLOBIN FROM A SAMPLE 审中-公开
标题翻译：从样品中消除胆固醇糖蛋白的方法
公开(公告)号：WO1994008242A1
公开(公告)日：1994-04-14
申请号：PCT/GB1993002019
申请日：1993-09-28
申请人： DREW SCIENTIFIC LTD. , KENNEY, Andrew, Charles , ADEWUNMI, Maria, Dolapo
发明人： DREW SCIENTIFIC LTD.
IPC分类号： G01N33/72
CPC分类号： G01N33/723
摘要： A process for the elimination of labile glycohaemoglobin from a sample comprises adding to the sample a reagent comprising a temperature dependent buffer, and a method for measuring the concentration of stable glycohaemoglobin in a sample containing stable and labile glycohaemoglobin fractions.
摘要翻译：从样品中消除不稳定的糖型血红蛋白的方法包括向样品中加入包含温度依赖性缓冲液的试剂，以及测量含有稳定和不稳定的糖血红蛋白级分的样品中稳定的糖海马血红蛋白的浓度的方法。

2. 发明申请

WO1993017328A1 LIQUID CHROMATOGRAPHY APPARATUS 审中-公开
标题翻译：液相色谱仪
公开(公告)号：WO1993017328A1
公开(公告)日：1993-09-02
申请号：PCT/GB1993000339
申请日：1993-02-18
申请人： DREW SCIENTIFIC LIMITED , DREW, Keith, Raymond , KENNEY, Andrew, Charles
发明人： DREW SCIENTIFIC LIMITED
IPC分类号： G01N30/88
CPC分类号： B01L3/502738 , B01F13/0064 , B01F15/0256 , B01L3/50273 , B01L3/502753 , B01L2200/0605 , B01L2300/0816 , B01L2300/0867 , B01L2400/0406 , B01L2400/0478 , B01L2400/0622 , B01L2400/0644 , G01N30/88 , G01N2030/0095 , G01N2030/202 , G01N2030/324 , G01N2030/326 , G01N2030/347 , G01N2030/8881 , G01N2030/207
摘要： In a liquid chromatography apparatus comprising a reusable reader device for use with disposable chromatography cassettes, each cassette has a capillary (12) for drawing a sample into a valve (14) located between a mixing apparatus (4, 6) and the chromatography separation means (20). The valve has a predetermined internal volume such that on rotation a sample of fixed quantity is placed in line between the separation means (20) and the mixing apparatus (4, 6). The mixing apparatus has elongate chambers (4, 6) with moveable plugs (26) pushed by piston rams of the reader device to provide a combined flow of varying relative concentration. Alternatively, it has resiliently flexible elongate chambers (4, 6) of varying cross-section. When the cassette is inserted into the reader, rollers squeeze the chambers (4, 6) to provide the combined flow. The reader has a pulsed xenon tube and two photodetectors, one for measuring attenuation of sample fractions exiting the separation means (20) and the other the intensity of the light source, the difference between the two yielding the measurement.
摘要翻译：在包括与一次性色谱盒一起使用的可重复使用的读取器装置的液相色谱装置中，每个盒具有毛细管（12），用于将样品抽吸到位于混合装置（4,6）和色谱分离装置（20）。阀具有预定的内部体积，使得在旋转时，固定量的样品被置于分离装置（20）和混合装置（4,6）之间。混合装置具有细长的室（4,6），其具有由读取装置的活塞柱塞推动的可移动的塞子（26），以提供变化的相对浓度的组合流。或者，其具有变化的横截面的弹性柔性细长室（4,6）。当盒式磁带插入读取器中时，辊挤压室（4,6）以提供组合的流。读取器具有脉冲氙气管和两个光电检测器，一个用于测量离开分离装置（20）的样品分数的衰减，另一个用于测量光源的强度，两者之间的差异导致测量。

3. 发明申请

WO1988000615A1 PROCESS FOR PURIFYING A PLASMINOGEN ACTIVATOR 审中-公开
标题翻译：纯化激活剂的方法
公开(公告)号：WO1988000615A1
公开(公告)日：1988-01-28
申请号：PCT/GB1987000498
申请日：1987-07-15
申请人： CELLTECH LIMITED , KENNEY, Andrew, Charles , PERRY, Angela, Joyce , WHITE, Susan , MARSTON, Fiona, Angela, Olinda
发明人： CELLTECH LIMITED
IPC分类号： C12N09/64
CPC分类号： C12N9/6459 , C12Y304/21069
摘要： Process for the purification of tPA comprising: applying a mother solution containing tPA to a highly anionic chromatography medium (HACM) equilibrated with a buffer at 25mS ionic strength or lower and at a pH between 4 and 7; eluting the tPA from the highly anionic chromatography medium with a high ionic strength buffer; applying the eluate from the highly anionic chromatography medium to an affinity chromatography medium (ACM), comprising an anti-tPA antibody covalently bound to a chromatography material, equilibrated with a buffer at pH 5 to 10; and eluting the tPA from the affinity chromatography medium with a buffer which disrupts the binding of the antibody to the tPA. Preferably, the mother solution is the supernatant from a culture of a eukaryotic cell line which has been transformed by recombinant DNA technology so that it is able to express and secrete tPA. Advantageously the mother solution is processed as soon as it is harvested and all the process steps are carried out as quickly as possible, the mother solution contains a serine protease inhibitor, and all the solutions contain a detergent and are kept at as low a pH as possible.
摘要翻译：用于纯化tPA的方法，包括：将含有tPA的母液施加到用25mS离子强度或更低的pH在4至7之间的缓冲液平衡的高度阴离子色谱介质（HACM）上; 用高离子强度缓冲液从高度阴离子色谱介质中洗脱tPA; 将洗脱液从高度阴离子色谱介质施加到亲和层析介质（ACM），其包含与pH 5至10的缓冲液平衡的与色谱材料共价结合的抗tPA抗体; 并用缓冲液从亲和层析介质中洗脱tPA，破坏抗体与tPA的结合。优选地，母液是通过重组DNA技术转化的真核细胞系的培养物的上清液，使得其能够表达和分泌tPA。有利的是，一旦收获母液即可迅速处理母液，母液中含有丝氨酸蛋白酶抑制剂，所有溶液都含有洗涤剂，并保持在pH 可能。

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