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1. 发明申请

WO9835053A2 ELECTROCHEMICAL ANALYTE SENSORS USING THERMOSTABLE SOYBEAN PEROXIDASE 审中-公开
标题翻译：电化学分析仪使用热稳定的大豆过氧化物
公开(公告)号：WO9835053A2
公开(公告)日：1998-08-13
申请号：PCT/US9802403
申请日：1998-02-11
申请人： HELLER E & CO , HELLER ADAM , KENAUSIS GREGG L , CHEN QIANG , VREEKE MARK S
发明人： HELLER ADAM , KENAUSIS GREGG L , CHEN QIANG , VREEKE MARK S
IPC分类号： C12N15/09 , C12Q1/00 , C12Q1/28 , C12Q1/68 , G01N27/327
CPC分类号： C12Q1/6825 , C12Q1/005 , Y10S435/817
摘要： A sensor for the detection and measurement of an analyte in a biofluid. The sensor includes two enzymes. One type of sensor measures the concentration of hydrogen peroxide using a thermostable peroxidase enzyme that is immobilized in a redox hydrogel to form a sensing layer on a working electrode. This sensor also includes a hydrogen peroxide-generating second enzyme which is insulated from the redox hydrogel and electrode. This second enzyme generates hydrogen peroxide in response to the presence of an analyte or analyte-generated compound. The second enzyme may be insulated from the electrode by placement of an electrically insulating layer between the sensing layer and the second enzyme layer. Alternatively, the second enzyme is immobilized in an inorganic polymeric matrix, preferably made using a sol-gel polymerization process. Such matrices include those made of silica. Often, the second enzyme is stabilized by immobilization in a sol-gel. Further stabilization of polyelectrolytic enzymes can be obtained by immobilizing the enzyme with a polyelectrolytic polymer in the sol-gel matrix.
摘要翻译：用于检测和测量生物流体中分析物的传感器。传感器包括两种酶。一种传感器使用固定在氧化还原水凝胶中的热稳定过氧化物酶测量过氧化氢的浓度，以在工作电极上形成感测层。该传感器还包括与氧化还原水凝胶和电极绝缘的产生过氧化氢的第二酶。该第二酶响应于分析物或分析物产生的化合物的存在而产生过氧化氢。第二酶可以通过在感测层和第二酶层之间放置电绝缘层而与电极绝缘。或者，将第二酶固定在无机聚合物基质中，优选使用溶胶 - 凝胶聚合方法制备。这些基质包括由二氧化硅制成的那些。通常，通过固定在溶胶 - 凝胶中来稳定第二种酶。聚电解酶的进一步稳定可以通过在溶胶 - 凝胶基质中用聚电解质聚合物固定酶来获得。

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