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    • 3. 发明授权
    • Cell-culture apparatus
    • 细胞培养装置
    • US4774187A
    • 1988-09-27
    • US35334
    • 1987-04-07
    • Jurgen Lehmann
    • Jurgen Lehmann
    • C12M3/02C12M3/06C12M1/04
    • C12M27/02C12M29/04
    • The invention concerns an apparatus for growing cell cultures wherein the cultures are located in at least one liquid nutrient medium and the nutrient medium or media are introduced through at least one pump-driven feed line, and where a gas (oxygen, nitrogen, air or the like) is introduced into the head room above the liquid level of the closed container, the gas pressure being kept constant in the head room, this apparatus being designed in such a manner as to prevent effectively injury to the free cells perforce ejected when evacuating the nutrient media. To that end the invention provides an overflow line communicating with the liquid in the container and terminating outside this container, its free end being located so much above the liquid level in the container that the pressure generated by the liquid column in the overflow line balances the gas pressure in the container head room.
    • 本发明涉及用于培养细胞培养物的装置,其中培养物位于至少一种液体营养培养基中,并且营养培养基或培养基通过至少一个泵驱动进料管线引入,并且其中气体(氧气,氮气,空气或 类似物)被引入到密封容器的液面之上的头部房间中,气体压力在头部房间中保持恒定,该装置被设计成防止对排空时排出的游离细胞的有效损伤 营养介质。 为此,本发明提供了一种与容器中的液体连通并且终止在该容器外部的溢流管线,其自由端位于容器中的液面之上,使溢流管线中液柱产生的压力平衡 容器头部的气体压力。
    • 5. 发明授权
    • Process and apparatus for gassing liquids
    • 用于放液的工艺和设备
    • US4870018A
    • 1989-09-26
    • US855624
    • 1986-03-17
    • Jurgen Lehmann
    • Jurgen Lehmann
    • B01F3/04C12M1/04C12M1/06C12M3/00C12M3/02C12M3/06C12N5/00C12N5/07C12N5/071
    • C12M29/04B01F3/04531
    • In a process and an apparatus for the bubble-free gassing of liquids, especially of culture media for propagating tissue cultures, in which process and apparatus a gas exchange occurs across an immersed membrane sheet, to achieve simple, effective and as uniform as possible a distribution of the gas introduction and gas desorption without mechanical damage to the liquid constituents, a tubular membrane cage formed by hollow membrane filaments is suspended flexibly in the medium to be gassed and moved in a circular oscillating movement without true rotation. The gassing rate is controlled by altering the angular oscillating movement frequency and/or the radius of the circular path, or alternatively by altering the gas concentration and/or the pressure of the gas flowing into the membrane cage.
    • PCT No.PCT / EP85 / 00387 Sec。 371日期:1986年3月17日 102(e)1986年3月17日PCT PCT于1985年8月2日提交。在用于无气泡气体的方法和装置中,特别是用于传播组织培养物的培养基,其中气体交换 发生在浸没的膜片上,为了尽可能简单,有效和均匀地进行气体引入和气体解吸的分布而不会对液体成分造成机械损伤,由中空膜细丝形成的管状膜笼可以柔性地悬挂在介质中 在没有真正旋转的情况下进行循环振荡运动。 通过改变角振荡运动频率和/或圆形路径的半径,或者通过改变流入膜笼的气体的气体浓度和/或压力来控制放气速率。