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1. 发明申请

US20080216199A1 Molybdenum Transporter and Gene Thereof 有权
标题翻译：钼转运体及其基因
公开(公告)号：US20080216199A1
公开(公告)日：2008-09-04
申请号：US11908537
申请日：2006-03-14
申请人： Toru Fujiwara , Junpei Takano , Hajime Tomatsu
发明人： Toru Fujiwara , Junpei Takano , Hajime Tomatsu
IPC分类号： A01H5/00 , C07H21/04 , C07K14/415 , C12Q1/02 , C12N15/63 , C12N1/19
CPC分类号： G01N33/5097 , C07K14/415 , C12N15/8261 , G01N2333/415 , Y02A40/146
摘要： It is to provide a novel molybdenum ion transporter MoTR1 gene responsible for molybdenum transport for the first time in plants, enabling to promote effectively molybdenum absorption from the environment or molybdenum transport in vivo. By QTL analysis of Arabidopsis thaliana accessions Col-0 and Ler, it was found that QTL which dominates Mo concentration in leaves was present on the chromosome No: 2. In the present invention, the region in which the causal gene is present was limited to 172 kb, by a genetic analysis. In the region, the gene At2g25680 having a domain common to sulfate ion transporter, while its function is not revealed was present. Thus, knockout strains of 2 separate lines in which a foreign gene fragment (T-DNA) was introduced at At2g25680 were obtained, Mo concentration in leaves was measured, and MoTR1 gene was identified.
摘要翻译：它是在植物中首次提供负责钼运输的新型钼离子转运体MoTR1基因，能够有效地促进来自环境的钼吸收或体内钼转运。通过拟南芥参考Col-0和Ler的QTL分析，发现叶片中Mo浓度主导的QTL存在于染色体No.2上。在本发明中，存在致病基因的区域限于 172 kb，通过遗传分析。在该区域中，存在具有与硫酸根离子转运体共同的结构域的At2g25680基因，而其功能尚未显露。因此，获得其中在At2g25680引入外源基因片段（T-DNA）的2条分离株的敲除菌株，测定叶中的Mo浓度，并鉴定出MoTR1基因。

2. 发明授权

US07772461B2 Molybdenum transporter gene and use thereof 有权
标题翻译：钼转运蛋白基因及其用途
公开(公告)号：US07772461B2
公开(公告)日：2010-08-10
申请号：US11908537
申请日：2006-03-14
申请人： Toru Fujiwara , Junpei Takano , Hajime Tomatsu
发明人： Toru Fujiwara , Junpei Takano , Hajime Tomatsu
IPC分类号： C12N15/09 , C12N15/82 , A01H5/00
CPC分类号： G01N33/5097 , C07K14/415 , C12N15/8261 , G01N2333/415 , Y02A40/146
摘要： It is to provide a novel molybdenum ion transporter MoTR1 gene responsible for molybdenum transport for the first time in plants, enabling to promote effectively molybdenum absorption from the environment or molybdenum transport in vivo. By QTL analysis of Arabidopsis thaliana accessions Col-0 and Ler, it was found that QTL which dominates Mo concentration in leaves was present on the chromosome No: 2. In the present invention, the region in which the causal gene is present was limited to 172 kb, by a genetic analysis. In the region, the gene At2g25680 having a domain common to sulfate ion transporter, while its function is not revealed was present. Thus, knockout strains of 2 separate lines in which a foreign gene fragment (T-DNA) was introduced at At2g25680 were obtained, Mo concentration in leaves was measured, and MoTR1 gene was identified.
摘要翻译：它是在植物中首次提供负责钼运输的新型钼离子转运体MoTR1基因，能够有效地促进来自环境的钼吸收或体内钼转运。通过拟南芥参考Col-0和Ler的QTL分析，发现叶片中Mo浓度主导的QTL存在于染色体No.2上。在本发明中，存在致病基因的区域限于 172 kb，通过遗传分析。在该区域中，存在具有与硫酸根离子转运体共同的结构域的At2g25680基因，而其功能尚未显露。因此，获得其中在At2g25680引入外源基因片段（T-DNA）的2条分离株的敲除菌株，测定叶中的Mo浓度，并鉴定出MoTR1基因。

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