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1. 发明授权

US09096872B2 Recombinant microorganisms having increased ability to produce butanol and method of producing butanol using the same 有权
标题翻译：具有提高丁醇生产能力的重组微生物和使用其生产丁醇的方法
公开(公告)号：US09096872B2
公开(公告)日：2015-08-04
申请号：US13497743
申请日：2010-09-21
申请人： Sang Yup Lee , Yu-Sin Jang , Jin Young Lee , Joung Min Lee , Jin Hwan Park
发明人： Sang Yup Lee , Yu-Sin Jang , Jin Young Lee , Joung Min Lee , Jin Hwan Park
IPC分类号： C12N1/21 , C12P7/16 , C12N9/04 , C12N9/10
CPC分类号： C12P7/16 , C12N9/0006 , C12N9/1029 , C12Y101/01001 , C12Y203/01008 , C12Y203/01019 , Y02E50/10
摘要： The present invention relates to recombinant microorganisms having an increased ability to produce butanol, and a method of producing butanol using the same. More specifically, the invention relates to recombinant microorganisms whose ability to produce butanol was increased by manipulation of their metabolic networks, and a method of producing butanol using the same. The recombinant microorganisms having an increased ability to produce butanol comprise a deletion of a gene, which encodes an enzyme that converts acetyl CoA to acetate, in host microorganisms having genes that encode enzymes involved in acetyl CoA and butyryl CoA biosynthetic pathway. The recombinant microorganisms obtained by manipulating the metabolic flux of microorganisms are able to selectively produce butanol with high efficiency, and thus are useful as microorganisms for producing industrial solvents and transportation fuels.
摘要翻译：本发明涉及生产丁醇的能力提高的重组微生物，以及使用其制备丁醇的方法。更具体地，本发明涉及通过操作其代谢网络来增加生产丁醇的能力的重组微生物，以及使用其生产丁醇的方法。在具有编码参与乙酰辅酶A和丁酰辅酶A生物合成途径的酶的基因的宿主微生物中，具有增加的产生丁醇能力的重组微生物包括编码转化乙酰CoA至乙酸的酶的基因的缺失。通过操作微生物的代谢通过获得的重组微生物能够以高效率选择性地生产丁醇，因此可用作生产工业溶剂和运输燃料的微生物。

2. 发明授权

US08765446B2 Recombinant mutant microorganisms having increased ability to produce alcohols and method of producing alcohols using the same 有权
标题翻译：具有增加生产醇的能力的重组突变体微生物和使用其制备醇的方法
公开(公告)号：US08765446B2
公开(公告)日：2014-07-01
申请号：US13497736
申请日：2010-09-21
申请人： Sang Yup Lee , Yu-Sin Jang , Joung Min Lee , Jung Ae Im , Hyohak Song
发明人： Sang Yup Lee , Yu-Sin Jang , Joung Min Lee , Jung Ae Im , Hyohak Song
IPC分类号： C12N1/20 , C12N15/74 , C12N9/02 , C12N15/00 , C12P7/16 , C12Q1/26 , C07H21/04
CPC分类号： C12N9/13 , C12N9/0006 , C12N9/88 , C12N15/74 , C12P3/00 , C12P7/04 , C12P7/06 , C12P7/16 , C12P7/26 , C12P7/28 , C12P7/52 , C12P7/54 , C12P7/56 , Y02E50/10 , Y02E50/17
摘要： The present invention relates to recombinant mutant microorganisms having an increased ability to produce alcohol and a method of producing alcohol using the same, and more particularly to recombinant mutant microorganisms which have an increased ability to produce butanol, ethanol, isopropanol or mixed alcohols, which can be used as fuel, while producing little or no producing acetone as a byproduct, and to a method of producing butanol, ethanol, isopropanol or mixed alcohols using the same. The inventive recombinant mutant microorganisms having an increased ability to produce butanol or mixed alcohols and to remove acetone are those in which genes that encode enzymes involved in producing butanol from butyryl-CoA or butylaldehyde and in producing isopropanol from acetone were amplified or introduced in host microorganisms. The recombinant mutant microorganisms produce little or no byproducts such as acetone and can have an increased ability to produce alcohols, as a result of manipulating metabolic pathways. Thus, the recombinant mutant microorganisms are useful for industrial production of butanol or mixed alcohols comprising butanol and isopropanol.
摘要翻译：本发明涉及具有增加的产生醇的能力的重组突变微生物和使用其的醇的制备方法，更具体地说涉及生产丁醇，乙醇，异丙醇或混合醇的能力增加的重组突变微生物，其可以作为燃料使用，同时产生很少或不产生丙酮作为副产物，以及使用其制备丁醇，乙醇，异丙醇或混合醇的方法。生产丁醇或混合醇并除去丙酮的能力增强的本发明的重组突变微生物是那些编码参与从丁酰辅酶A或丁醛生产丁醇和从丙酮生产异丙醇的酶的基因被扩增或引入宿主微生物。由于操纵代谢途径，重组突变体微生物产生很少或没有副产物如丙酮，并且可以具有增加的产生醇的能力。因此，重组突变微生物可用于工业生产丁醇或包含丁醇和异丙醇的混合醇。

3. 发明授权

US08835663B2 Lubricity improver 有权
标题翻译：润滑改进剂
公开(公告)号：US08835663B2
公开(公告)日：2014-09-16
申请号：US13548501
申请日：2012-07-13
申请人： Young-Kwan Lim , Joung Min Lee , Choong-Sub Jung , Jong-Ryeol Kim , Eui Soon Yim
发明人： Young-Kwan Lim , Joung Min Lee , Choong-Sub Jung , Jong-Ryeol Kim , Eui Soon Yim
IPC分类号： C07C51/00 , C07C59/00 , C10M129/02 , C10L1/19 , C07C69/708
CPC分类号： C07C69/708 , C10L1/19 , C10L2270/026 , C10M129/02 , C10M2207/04 , C10N2230/06 , C10N2230/64 , Y02P30/20 , C10M2207/281
摘要： Provided is a lubricity improver capable of improving lubricity and storage stability due to anti-oxidation, the lubricity improver containing a saturated fatty acid methyl ester derivative including at least one 1,2-dimethoxy ethylene structural unit represented by Chemical Formula a below, obtained by converting a double bond (olefin) of biodiesel, which is used as a lubricity improver of fuel, that is, the existing fatty acid methyl ester (FAME), into a dimethoxy group.
摘要翻译：提供一种润滑性改进剂，其能够提高由于抗氧化引起的润滑性和储存稳定性，所述润滑性改进剂含有饱和脂肪酸甲酯衍生物，所述饱和脂肪酸甲酯衍生物包含至少一种由以下化学式表示的1,2-二甲氧基乙烯结构单元，将用作燃料的润滑性改进剂即现有的脂肪酸甲酯（FAME）的生物柴油的双键（烯烃）转化成二甲氧基。

4. 发明申请

US20130053590A1 LUBRICITY IMPROVER 有权
标题翻译：润滑改进
公开(公告)号：US20130053590A1
公开(公告)日：2013-02-28
申请号：US13548501
申请日：2012-07-13
申请人： Young-Kwan Lim , Joung Min Lee , Choong-Sub Jung , Jong-Ryeol Kim , Eui Soon Yim
发明人： Young-Kwan Lim , Joung Min Lee , Choong-Sub Jung , Jong-Ryeol Kim , Eui Soon Yim
IPC分类号： C07C69/675 , C07C67/31
CPC分类号： C07C69/708 , C10L1/19 , C10L2270/026 , C10M129/02 , C10M2207/04 , C10N2230/06 , C10N2230/64 , Y02P30/20 , C10M2207/281
摘要： Provided is a lubricity improver capable of improving lubricity and storage stability due to anti-oxidation, the lubricity improver containing a saturated fatty acid methyl ester derivative including at least one 1,2-dimethoxy ethylene structural unit represented by Chemical Formula a below, obtained by converting a double bond (olefin) of biodiesel, which is used as a lubricity improver of fuel, that is, the existing fatty acid methyl ester (FAME), into a dimethoxy group.
摘要翻译：提供一种润滑性改进剂，其能够提高由于抗氧化引起的润滑性和储存稳定性，所述润滑性改进剂含有饱和脂肪酸甲酯衍生物，所述饱和脂肪酸甲酯衍生物包含至少一种由以下化学式表示的1,2-二甲氧基乙烯结构单元，将用作燃料的润滑性改进剂即现有的脂肪酸甲酯（FAME）的生物柴油的双键（烯烃）转化成二甲氧基。

5. 发明申请

US20130017588A1 RECOMBINANT MICROORGANISMS HAVING INCREASED ABILITY TO PRODUCE BUTANOL AND METHOD OF PRODUCING BUTANOL USING THE SAME 有权
标题翻译：具有生产丁醇的能力提高的重组微生物和使用其的丁醇的生产方法
公开(公告)号：US20130017588A1
公开(公告)日：2013-01-17
申请号：US13497743
申请日：2010-09-21
申请人： Sang Yup Lee , Yu-Sin Jang , Jin Young Lee , Joung Min Lee , Jin Hwan Park
发明人： Sang Yup Lee , Yu-Sin Jang , Jin Young Lee , Joung Min Lee , Jin Hwan Park
IPC分类号： C12N1/21 , C12P7/16 , C12N15/01
CPC分类号： C12P7/16 , C12N9/0006 , C12N9/1029 , C12Y101/01001 , C12Y203/01008 , C12Y203/01019 , Y02E50/10
摘要： The present invention relates to recombinant microorganisms having an increased ability to produce butanol, and a method of producing butanol using the same. More specifically, the invention relates to recombinant microorganisms whose ability to produce butanol was increased by manipulation of their metabolic networks, and a method of producing butanol using the same. The recombinant microorganisms having an increased ability to produce butanol comprise a deletion of a gene, which encodes an enzyme that converts acetyl CoA to acetate, in host microorganisms having genes that encode enzymes involved in acetyl CoA and butyryl CoA biosynthetic pathway. The recombinant microorganisms obtained by manipulating the metabolic flux of microorganisms are able to selectively produce butanol with high efficiency, and thus are useful as microorganisms for producing industrial solvents and transportation fuels.
摘要翻译：本发明涉及生产丁醇的能力提高的重组微生物，以及使用其制备丁醇的方法。更具体地，本发明涉及通过操作其代谢网络来增加生产丁醇的能力的重组微生物，以及使用其生产丁醇的方法。在具有编码参与乙酰辅酶A和丁酰辅酶A生物合成途径的酶的基因的宿主微生物中，具有增加的产生丁醇能力的重组微生物包括编码转化乙酰CoA至乙酸的酶的基因的缺失。通过操作微生物的代谢通过获得的重组微生物能够高效地选择性地生产丁醇，因此可用作生产工业溶剂和运输燃料的微生物。

6. 发明申请

US20120301936A1 RECOMBINANT MUTANT MICROORGANISMS HAVING INCREASED ABILITY TO PRODUCE ALCOHOLS AND METHOD OF PRODUCING ALCOHOLS USING THE SAME 有权
标题翻译：具有增加生产酒精的能力的重组突变体微生物和使用该醇的生产醇的方法
公开(公告)号：US20120301936A1
公开(公告)日：2012-11-29
申请号：US13497736
申请日：2010-09-21
申请人： Sang Yup Lee , Yu-Sin Jang , Joung Min Lee , Jung Ae Im , Hyohak Song
发明人： Sang Yup Lee , Yu-Sin Jang , Joung Min Lee , Jung Ae Im , Hyohak Song
IPC分类号： C12P7/16 , C12N1/21 , C12N15/74
CPC分类号： C12N9/13 , C12N9/0006 , C12N9/88 , C12N15/74 , C12P3/00 , C12P7/04 , C12P7/06 , C12P7/16 , C12P7/26 , C12P7/28 , C12P7/52 , C12P7/54 , C12P7/56 , Y02E50/10 , Y02E50/17
摘要： The present invention relates to recombinant mutant microorganisms having an increased ability to produce alcohol and a method of producing alcohol using the same, and more particularly to recombinant mutant microorganisms which have an increased ability to produce butanol, ethanol, isopropanol or mixed alcohols, which can be used as fuel, while producing little or no producing acetone as a byproduct, and to a method of producing butanol, ethanol, isopropanol or mixed alcohols using the same. The inventive recombinant mutant microorganisms having an increased ability to produce butanol or mixed alcohols and to remove acetone are those in which genes that encode enzymes involved in producing butanol from butyryl-CoA or butylaldehyde and in producing isopropanol from acetone were amplified or introduced in host microorganisms. The recombinant mutant microorganisms produce little or no byproducts such as acetone and can have an increased ability to produce alcohols, as a result of manipulating metabolic pathways. Thus, the recombinant mutant microorganisms are useful for industrial production of butanol or mixed alcohols comprising butanol and isopropanol.
摘要翻译：本发明涉及具有增加的产生醇的能力的重组突变微生物和使用其的醇的制备方法，更具体地说涉及生产丁醇，乙醇，异丙醇或混合醇的能力增加的重组突变微生物，其可以作为燃料使用，同时产生很少或不产生丙酮作为副产物，以及使用其制备丁醇，乙醇，异丙醇或混合醇的方法。生产丁醇或混合醇并除去丙酮的能力增强的本发明的重组突变微生物是那些编码参与从丁酰辅酶A或丁醛生产丁醇和从丙酮生产异丙醇的酶的基因被扩增或引入宿主微生物。由于操纵代谢途径，重组突变体微生物产生很少或没有副产物如丙酮，并且可以具有增加的产生醇的能力。因此，重组突变微生物可用于工业生产丁醇或包含丁醇和异丙醇的混合醇。

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